Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3486 and Pf1N1B4_3487 are separated by 340 nucleotides Pf1N1B4_3487 and Pf1N1B4_3488 are separated by 145 nucleotides
Pf1N1B4_3486 - Transcriptional regulator, AraC family, at 3,893,011 to 3,894,024
_3486
Pf1N1B4_3487 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate, at 3,894,365 to 3,895,699
_3487
Pf1N1B4_3488 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 3,895,845 to 3,897,026
_3488
Position (kb)
3894
3895
3896 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3893.399 kb on - strand, within Pf1N1B4_3486 at 3893.501 kb on + strand, within Pf1N1B4_3486 at 3893.718 kb on - strand, within Pf1N1B4_3486 at 3893.828 kb on + strand, within Pf1N1B4_3486 at 3893.828 kb on + strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3894.024 kb on + strand at 3894.025 kb on - strand at 3894.177 kb on + strand at 3894.338 kb on + strand at 3894.338 kb on + strand at 3894.362 kb on - strand at 3894.362 kb on - strand at 3894.362 kb on - strand at 3894.386 kb on + strand at 3894.387 kb on - strand at 3894.388 kb on + strand at 3894.389 kb on - strand at 3894.399 kb on - strand at 3894.536 kb on - strand, within Pf1N1B4_3487 at 3894.608 kb on + strand at 3894.609 kb on - strand, within Pf1N1B4_3487 at 3894.609 kb on - strand, within Pf1N1B4_3487 at 3895.001 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.050 kb on - strand at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.133 kb on + strand at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.333 kb on + strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.650 kb on + strand at 3895.651 kb on - strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.701 kb on + strand at 3895.702 kb on - strand at 3895.702 kb on - strand at 3895.814 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3896.025 kb on + strand at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.268 kb on + strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.295 kb on + strand, within Pf1N1B4_3488 at 3896.296 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand at 3896.554 kb on - strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,893,399 - Pf1N1B4_3486 0.38 +0.1 3,893,501 + Pf1N1B4_3486 0.48 -0.2 3,893,718 - Pf1N1B4_3486 0.70 -0.5 3,893,828 + Pf1N1B4_3486 0.81 +0.4 3,893,828 + Pf1N1B4_3486 0.81 +1.4 3,893,829 - Pf1N1B4_3486 0.81 -1.7 3,893,829 - Pf1N1B4_3486 0.81 -0.9 3,893,829 - Pf1N1B4_3486 0.81 -0.9 3,894,024 + +1.3 3,894,025 - +0.7 3,894,177 + +0.3 3,894,338 + +0.3 3,894,338 + +1.0 3,894,362 - -0.5 3,894,362 - +0.4 3,894,362 - -0.5 3,894,386 + +0.2 3,894,387 - -0.9 3,894,388 + -0.6 3,894,389 - -0.4 3,894,399 - +0.4 3,894,536 - Pf1N1B4_3487 0.13 +1.4 3,894,608 + -0.9 3,894,609 - Pf1N1B4_3487 0.18 +0.1 3,894,609 - Pf1N1B4_3487 0.18 -2.6 3,895,001 + Pf1N1B4_3487 0.48 -0.5 3,895,049 + Pf1N1B4_3487 0.51 +1.4 3,895,049 + Pf1N1B4_3487 0.51 +1.1 3,895,049 + Pf1N1B4_3487 0.51 -1.6 3,895,049 + Pf1N1B4_3487 0.51 +0.2 3,895,049 + Pf1N1B4_3487 0.51 +0.4 3,895,049 + Pf1N1B4_3487 0.51 +0.2 3,895,049 + Pf1N1B4_3487 0.51 +1.5 3,895,049 + Pf1N1B4_3487 0.51 +1.0 3,895,049 + Pf1N1B4_3487 0.51 -1.3 3,895,049 + Pf1N1B4_3487 0.51 +0.3 3,895,049 + Pf1N1B4_3487 0.51 +1.7 3,895,049 + Pf1N1B4_3487 0.51 +0.4 3,895,049 + Pf1N1B4_3487 0.51 +1.5 3,895,049 + Pf1N1B4_3487 0.51 +1.7 3,895,049 + Pf1N1B4_3487 0.51 +0.7 3,895,049 + Pf1N1B4_3487 0.51 -0.3 3,895,050 - -1.3 3,895,050 - Pf1N1B4_3487 0.51 +1.7 3,895,050 - Pf1N1B4_3487 0.51 -0.1 3,895,050 - Pf1N1B4_3487 0.51 +1.4 3,895,133 + +0.1 3,895,283 - Pf1N1B4_3487 0.69 -0.0 3,895,283 - Pf1N1B4_3487 0.69 -0.6 3,895,292 + Pf1N1B4_3487 0.69 -2.5 3,895,292 + Pf1N1B4_3487 0.69 +1.3 3,895,293 - Pf1N1B4_3487 0.70 -1.6 3,895,293 - Pf1N1B4_3487 0.70 -1.9 3,895,333 + Pf1N1B4_3487 0.73 -0.6 3,895,334 - Pf1N1B4_3487 0.73 -0.3 3,895,334 - Pf1N1B4_3487 0.73 +0.1 3,895,650 + -0.9 3,895,651 - +0.7 3,895,697 + +0.0 3,895,697 + -0.3 3,895,697 + -1.6 3,895,697 + -0.9 3,895,697 + -1.9 3,895,697 + +0.9 3,895,697 + -0.9 3,895,697 + +0.0 3,895,697 + +0.2 3,895,697 + -0.5 3,895,697 + -2.3 3,895,698 - +0.7 3,895,698 - +0.7 3,895,698 - -0.6 3,895,698 - +0.4 3,895,698 - -0.3 3,895,698 - +0.7 3,895,701 + +1.7 3,895,702 - +1.0 3,895,702 - -0.9 3,895,814 + +2.9 3,895,844 + -0.3 3,895,844 + +1.9 3,895,844 + -0.0 3,895,845 - -0.9 3,895,845 - +1.2 3,895,845 - +0.6 3,895,845 - -0.9 3,895,845 - -0.3 3,895,845 - -0.9 3,895,845 - +0.7 3,895,845 - +1.0 3,895,845 - -1.3 3,895,845 - -0.4 3,895,845 - +0.7 3,895,845 - +1.7 3,895,845 - +0.9 3,895,845 - +0.5 3,895,845 - -0.3 3,896,025 + +0.7 3,896,025 + Pf1N1B4_3488 0.15 +0.7 3,896,025 + Pf1N1B4_3488 0.15 -1.1 3,896,202 + Pf1N1B4_3488 0.30 +0.3 3,896,202 + Pf1N1B4_3488 0.30 -1.9 3,896,202 + Pf1N1B4_3488 0.30 +0.1 3,896,202 + Pf1N1B4_3488 0.30 +0.7 3,896,202 + Pf1N1B4_3488 0.30 -0.9 3,896,202 + -0.3 3,896,202 + Pf1N1B4_3488 0.30 +0.4 3,896,202 + Pf1N1B4_3488 0.30 -1.1 3,896,202 + Pf1N1B4_3488 0.30 +1.4 3,896,203 - Pf1N1B4_3488 0.30 -0.9 3,896,203 - Pf1N1B4_3488 0.30 +1.7 3,896,203 - Pf1N1B4_3488 0.30 -0.3 3,896,238 + Pf1N1B4_3488 0.33 -1.3 3,896,238 + Pf1N1B4_3488 0.33 -0.9 3,896,239 - Pf1N1B4_3488 0.33 -0.5 3,896,239 - Pf1N1B4_3488 0.33 -1.5 3,896,268 + Pf1N1B4_3488 0.36 +0.4 3,896,269 - Pf1N1B4_3488 0.36 -0.9 3,896,269 - Pf1N1B4_3488 0.36 +1.4 3,896,295 + Pf1N1B4_3488 0.38 +0.1 3,896,296 - Pf1N1B4_3488 0.38 -0.0 3,896,548 - Pf1N1B4_3488 0.59 -1.6 3,896,548 - Pf1N1B4_3488 0.59 +0.7 3,896,548 - Pf1N1B4_3488 0.59 -2.0 3,896,553 + Pf1N1B4_3488 0.60 +1.2 3,896,553 + Pf1N1B4_3488 0.60 -0.5 3,896,553 + -0.9 3,896,554 - Pf1N1B4_3488 0.60 -0.5 3,896,556 + Pf1N1B4_3488 0.60 +0.4 3,896,556 + Pf1N1B4_3488 0.60 +0.7
Or see this region's nucleotide sequence