Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3450 and Pf1N1B4_3451 are separated by 90 nucleotides Pf1N1B4_3451 and Pf1N1B4_3452 are separated by 186 nucleotides Pf1N1B4_3452 and Pf1N1B4_5221 are separated by 62 nucleotides Pf1N1B4_5221 and Pf1N1B4_5222 are separated by 110 nucleotides Pf1N1B4_5222 and Pf1N1B4_5223 are separated by 74 nucleotides Pf1N1B4_5223 and Pf1N1B4_3453 are separated by 22 nucleotides
Pf1N1B4_3450 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 3,845,825 to 3,848,446
_3450
Pf1N1B4_3451 - Aspartokinase (EC 2.7.2.4), at 3,848,537 to 3,849,778
_3451
Pf1N1B4_3452 - Carbon storage regulator, at 3,849,965 to 3,850,153
_3452
Pf1N1B4_5221 - tRNA-Ser-GCT, at 3,850,216 to 3,850,303
_5221
Pf1N1B4_5222 - tRNA-Arg-ACG, at 3,850,414 to 3,850,487
_5222
Pf1N1B4_5223 - tRNA-Arg-ACG, at 3,850,562 to 3,850,635
_5223
Pf1N1B4_3453 - hypothetical protein, at 3,850,658 to 3,850,834
_3453
Position (kb)
3848
3849
3850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.501 kb on + strand at 3848.501 kb on + strand at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.776 kb on + strand at 3849.776 kb on + strand at 3849.776 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.880 kb on - strand at 3849.964 kb on + strand at 3850.109 kb on + strand, within Pf1N1B4_3452 at 3850.109 kb on + strand, within Pf1N1B4_3452 at 3850.308 kb on + strand at 3850.308 kb on + strand at 3850.316 kb on + strand at 3850.493 kb on + strand at 3850.493 kb on + strand at 3850.493 kb on + strand at 3850.547 kb on + strand at 3850.549 kb on + strand at 3850.549 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,848,430 + -0.1 3,848,430 + +0.2 3,848,430 + +0.1 3,848,430 + +0.2 3,848,442 + +0.1 3,848,442 + -0.9 3,848,442 + +1.5 3,848,442 + -0.1 3,848,442 + -0.5 3,848,442 + -0.6 3,848,442 + -1.1 3,848,442 + -0.8 3,848,442 + +0.3 3,848,444 + -0.6 3,848,444 + +1.7 3,848,444 + +1.3 3,848,444 + -0.3 3,848,444 + +0.2 3,848,444 + +0.5 3,848,501 + -0.5 3,848,501 + -0.5 3,849,262 + Pf1N1B4_3451 0.58 -0.6 3,849,262 + Pf1N1B4_3451 0.58 +1.8 3,849,262 + Pf1N1B4_3451 0.58 -0.9 3,849,262 + Pf1N1B4_3451 0.58 -0.9 3,849,262 + Pf1N1B4_3451 0.58 -2.1 3,849,265 + Pf1N1B4_3451 0.59 -0.4 3,849,265 + Pf1N1B4_3451 0.59 -1.0 3,849,265 + Pf1N1B4_3451 0.59 -0.5 3,849,265 + Pf1N1B4_3451 0.59 -0.9 3,849,265 + Pf1N1B4_3451 0.59 -0.9 3,849,265 + Pf1N1B4_3451 0.59 -1.9 3,849,776 + -0.5 3,849,776 + +0.1 3,849,776 + +0.3 3,849,879 + -0.3 3,849,879 + +0.5 3,849,879 + -0.7 3,849,879 + +0.1 3,849,879 + -0.5 3,849,879 + -0.3 3,849,880 - -0.3 3,849,964 + -0.9 3,850,109 + Pf1N1B4_3452 0.76 +1.7 3,850,109 + Pf1N1B4_3452 0.76 -1.3 3,850,308 + +0.7 3,850,308 + -2.9 3,850,316 + -0.0 3,850,493 + -0.3 3,850,493 + -0.1 3,850,493 + +1.5 3,850,547 + +1.6 3,850,549 + -2.1 3,850,549 + -0.6
Or see this region's nucleotide sequence