Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3335 and Pf1N1B4_5220 are separated by 83 nucleotides Pf1N1B4_5220 and Pf1N1B4_3336 are separated by 333 nucleotides Pf1N1B4_3336 and Pf1N1B4_3337 are separated by 163 nucleotides Pf1N1B4_3337 and Pf1N1B4_3338 are separated by 53 nucleotides Pf1N1B4_3338 and Pf1N1B4_3339 are separated by 3 nucleotides
Pf1N1B4_3335 - Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 3,722,596 to 3,723,309
_3335
Pf1N1B4_5220 - tRNA-Pseudo-CGA, at 3,723,393 to 3,723,479
_5220
Pf1N1B4_3336 - UPF0339 protein NMA1193/NMA1859, at 3,723,813 to 3,724,004
_3336
Pf1N1B4_3337 - Transcriptional regulator, LysR family, at 3,724,168 to 3,725,097
_3337
Pf1N1B4_3338 - Probable 5-carboxymethyl-2-hydroxymuconate delta isomerase, at 3,725,151 to 3,725,516
_3338
Pf1N1B4_3339 - Transcriptional regulator, LysR family, at 3,725,520 to 3,726,455
_3339
Position (kb)
3724
3725
3726 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3723.327 kb on + strand at 3723.327 kb on + strand at 3723.377 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.499 kb on + strand at 3723.511 kb on + strand at 3723.606 kb on + strand at 3723.615 kb on + strand at 3723.636 kb on + strand at 3723.655 kb on + strand at 3723.655 kb on + strand at 3723.656 kb on - strand at 3723.656 kb on - strand at 3723.671 kb on + strand at 3723.671 kb on + strand at 3723.672 kb on - strand at 3723.672 kb on - strand at 3723.672 kb on - strand at 3723.672 kb on - strand at 3723.703 kb on - strand at 3723.703 kb on - strand at 3723.713 kb on + strand at 3723.756 kb on + strand at 3723.756 kb on + strand at 3723.756 kb on + strand at 3723.757 kb on - strand at 3723.757 kb on - strand at 3723.757 kb on - strand at 3723.816 kb on + strand at 3723.816 kb on + strand at 3723.817 kb on - strand at 3723.817 kb on - strand at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand at 3723.924 kb on + strand at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.001 kb on - strand at 3724.001 kb on - strand at 3724.001 kb on - strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.063 kb on - strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.109 kb on + strand at 3724.109 kb on + strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.580 kb on - strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.692 kb on + strand, within Pf1N1B4_3337 at 3724.692 kb on + strand, within Pf1N1B4_3337 at 3724.693 kb on - strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.821 kb on + strand, within Pf1N1B4_3337 at 3724.821 kb on + strand, within Pf1N1B4_3337 at 3724.821 kb on + strand at 3724.833 kb on - strand, within Pf1N1B4_3337 at 3724.833 kb on - strand, within Pf1N1B4_3337 at 3724.909 kb on + strand, within Pf1N1B4_3337 at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.117 kb on + strand at 3725.117 kb on + strand at 3725.118 kb on - strand at 3725.172 kb on + strand at 3725.172 kb on + strand at 3725.172 kb on + strand at 3725.172 kb on + strand at 3725.173 kb on - strand at 3725.490 kb on + strand at 3725.490 kb on + strand at 3725.491 kb on - strand at 3725.491 kb on - strand at 3725.491 kb on - strand at 3725.747 kb on + strand, within Pf1N1B4_3339 at 3725.749 kb on + strand, within Pf1N1B4_3339 at 3725.750 kb on - strand, within Pf1N1B4_3339 at 3725.750 kb on - strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.770 kb on - strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.814 kb on - strand, within Pf1N1B4_3339
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,723,327 + +0.8 3,723,327 + -0.6 3,723,377 + +1.4 3,723,485 + +0.2 3,723,485 + -0.5 3,723,485 + +0.5 3,723,485 + -0.8 3,723,485 + -2.2 3,723,485 + +0.1 3,723,485 + +0.3 3,723,487 + +0.8 3,723,487 + -1.6 3,723,487 + -1.4 3,723,487 + +1.0 3,723,499 + -1.1 3,723,511 + +2.4 3,723,606 + +0.2 3,723,615 + +0.8 3,723,636 + -1.4 3,723,655 + +0.2 3,723,655 + +1.0 3,723,656 - -0.2 3,723,656 - -0.2 3,723,671 + -1.2 3,723,671 + -0.0 3,723,672 - -2.4 3,723,672 - -0.1 3,723,672 - +1.2 3,723,672 - -0.4 3,723,703 - -0.3 3,723,703 - +0.2 3,723,713 + +1.5 3,723,756 + +1.2 3,723,756 + +0.5 3,723,756 + -0.7 3,723,757 - +0.2 3,723,757 - +2.1 3,723,757 - -0.2 3,723,816 + -1.7 3,723,816 + +2.4 3,723,817 - +0.2 3,723,817 - +0.6 3,723,924 + Pf1N1B4_3336 0.58 +0.2 3,723,924 + Pf1N1B4_3336 0.58 +1.0 3,723,924 + Pf1N1B4_3336 0.58 -0.2 3,723,924 + Pf1N1B4_3336 0.58 -0.0 3,723,924 + -1.2 3,723,924 + -0.2 3,723,924 + Pf1N1B4_3336 0.58 -0.1 3,723,925 - Pf1N1B4_3336 0.58 -1.2 3,723,925 - Pf1N1B4_3336 0.58 +1.5 3,723,925 - Pf1N1B4_3336 0.58 -0.9 3,723,925 - Pf1N1B4_3336 0.58 +0.8 3,723,925 - +1.8 3,723,925 - Pf1N1B4_3336 0.58 +1.4 3,723,925 - Pf1N1B4_3336 0.58 +0.4 3,723,925 - Pf1N1B4_3336 0.58 +0.6 3,723,925 - Pf1N1B4_3336 0.58 +1.5 3,724,000 + -1.8 3,724,000 + +0.5 3,724,000 + -0.2 3,724,000 + -0.2 3,724,000 + -0.2 3,724,001 - +1.1 3,724,001 - +0.8 3,724,001 - -1.0 3,724,002 + -1.0 3,724,002 + +0.2 3,724,002 + +0.5 3,724,002 + +1.6 3,724,002 + -0.0 3,724,002 + -0.2 3,724,002 + -0.2 3,724,002 + -0.3 3,724,002 + -2.2 3,724,002 + +0.5 3,724,002 + -1.0 3,724,002 + -0.4 3,724,002 + -0.2 3,724,002 + -0.4 3,724,002 + -0.2 3,724,002 + -0.3 3,724,003 - -0.2 3,724,003 - -0.2 3,724,003 - +0.0 3,724,003 - -0.2 3,724,003 - +0.4 3,724,003 - +0.2 3,724,003 - -0.4 3,724,003 - +0.2 3,724,003 - +0.7 3,724,003 - -1.5 3,724,063 - -0.0 3,724,064 + +1.2 3,724,064 + +0.3 3,724,064 + +0.5 3,724,064 + +0.4 3,724,064 + -0.4 3,724,064 + +0.8 3,724,064 + +0.2 3,724,065 - +0.4 3,724,065 - +0.3 3,724,065 - -0.8 3,724,065 - +1.3 3,724,065 - +0.8 3,724,065 - -0.0 3,724,065 - -1.2 3,724,065 - -0.1 3,724,065 - +2.1 3,724,109 + -1.5 3,724,109 + +1.4 3,724,110 - +1.1 3,724,110 - +0.2 3,724,110 - +1.8 3,724,110 - +0.4 3,724,110 - -1.2 3,724,580 - Pf1N1B4_3337 0.44 +0.2 3,724,626 + Pf1N1B4_3337 0.49 +1.1 3,724,626 + Pf1N1B4_3337 0.49 +0.2 3,724,626 + Pf1N1B4_3337 0.49 +0.5 3,724,626 + Pf1N1B4_3337 0.49 +0.6 3,724,692 + Pf1N1B4_3337 0.56 +2.0 3,724,692 + Pf1N1B4_3337 0.56 -0.5 3,724,693 - Pf1N1B4_3337 0.56 -0.0 3,724,738 + Pf1N1B4_3337 0.61 +1.7 3,724,738 + Pf1N1B4_3337 0.61 -0.1 3,724,738 + Pf1N1B4_3337 0.61 +0.8 3,724,738 + Pf1N1B4_3337 0.61 +0.0 3,724,738 + Pf1N1B4_3337 0.61 -0.8 3,724,821 + Pf1N1B4_3337 0.70 -0.2 3,724,821 + Pf1N1B4_3337 0.70 +0.5 3,724,821 + +0.8 3,724,833 - Pf1N1B4_3337 0.72 +1.8 3,724,833 - Pf1N1B4_3337 0.72 -0.4 3,724,909 + Pf1N1B4_3337 0.80 +0.7 3,725,111 + -1.5 3,725,111 + -0.8 3,725,111 + +1.5 3,725,111 + -0.7 3,725,111 + -0.8 3,725,111 + -0.2 3,725,111 + -0.4 3,725,112 - -0.8 3,725,112 - -0.8 3,725,112 - -1.0 3,725,112 - +1.4 3,725,112 - +0.5 3,725,112 - +0.4 3,725,113 + -0.2 3,725,113 + -0.5 3,725,113 + +0.3 3,725,113 + +0.2 3,725,113 + +0.5 3,725,113 + +0.2 3,725,113 + +0.5 3,725,113 + +1.4 3,725,113 + -0.3 3,725,113 + +0.6 3,725,113 + -2.0 3,725,113 + -0.4 3,725,113 + +0.3 3,725,113 + -1.5 3,725,113 + +0.8 3,725,113 + -0.3 3,725,113 + +2.4 3,725,113 + -1.2 3,725,113 + +0.8 3,725,113 + +0.4 3,725,113 + -0.5 3,725,113 + -0.2 3,725,113 + +0.5 3,725,113 + +0.6 3,725,113 + +1.2 3,725,113 + -2.8 3,725,113 + -0.0 3,725,113 + +0.4 3,725,113 + +0.4 3,725,113 + -0.2 3,725,114 - -0.2 3,725,114 - +0.2 3,725,114 - -1.2 3,725,114 - +0.8 3,725,114 - -0.5 3,725,114 - +1.6 3,725,114 - -1.0 3,725,114 - +1.5 3,725,114 - -0.0 3,725,114 - -0.5 3,725,114 - -0.6 3,725,114 - -0.8 3,725,114 - +2.4 3,725,114 - +0.6 3,725,114 - +0.8 3,725,117 + -0.5 3,725,117 + -0.5 3,725,118 - +1.5 3,725,172 + +0.5 3,725,172 + +0.0 3,725,172 + -0.1 3,725,172 + +0.8 3,725,173 - -0.3 3,725,490 + -0.8 3,725,490 + +0.8 3,725,491 - +1.1 3,725,491 - +1.8 3,725,491 - -0.8 3,725,747 + Pf1N1B4_3339 0.24 -1.5 3,725,749 + Pf1N1B4_3339 0.24 -0.9 3,725,750 - Pf1N1B4_3339 0.25 +2.0 3,725,750 - Pf1N1B4_3339 0.25 -0.9 3,725,769 + Pf1N1B4_3339 0.27 -0.2 3,725,769 + Pf1N1B4_3339 0.27 -0.5 3,725,769 + Pf1N1B4_3339 0.27 -1.0 3,725,770 - Pf1N1B4_3339 0.27 +0.2 3,725,813 + Pf1N1B4_3339 0.31 -0.2 3,725,813 + Pf1N1B4_3339 0.31 +0.2 3,725,813 + Pf1N1B4_3339 0.31 +1.2 3,725,814 - Pf1N1B4_3339 0.31 +0.6
Or see this region's nucleotide sequence