Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3316

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3314 and Pf1N1B4_3315 are separated by 18 nucleotidesPf1N1B4_3315 and Pf1N1B4_3316 are separated by 50 nucleotidesPf1N1B4_3316 and Pf1N1B4_3317 overlap by 8 nucleotides Pf1N1B4_3314 - Tricarboxylate porin OpdH, at 3,703,010 to 3,704,302 _3314 Pf1N1B4_3315 - hypothetical protein, at 3,704,321 to 3,704,473 _3315 Pf1N1B4_3316 - Tricarboxylate transport transcriptional regulator TctD, at 3,704,524 to 3,705,195 _3316 Pf1N1B4_3317 - Tricarboxylate transport sensor protein TctE, at 3,705,188 to 3,706,576 _3317 Position (kb) 3704 3705 3706Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3703.569 kb on + strand, within Pf1N1B4_3314at 3703.569 kb on + strand, within Pf1N1B4_3314at 3703.569 kb on + strand, within Pf1N1B4_3314at 3703.569 kb on + strandat 3703.569 kb on + strand, within Pf1N1B4_3314at 3703.570 kb on - strand, within Pf1N1B4_3314at 3703.626 kb on + strand, within Pf1N1B4_3314at 3703.923 kb on + strand, within Pf1N1B4_3314at 3704.001 kb on - strand, within Pf1N1B4_3314at 3704.145 kb on + strand, within Pf1N1B4_3314at 3704.145 kb on + strand, within Pf1N1B4_3314at 3704.145 kb on + strand, within Pf1N1B4_3314at 3704.146 kb on - strand, within Pf1N1B4_3314at 3704.146 kb on - strand, within Pf1N1B4_3314at 3704.146 kb on - strand, within Pf1N1B4_3314at 3704.146 kb on - strand, within Pf1N1B4_3314at 3704.146 kb on - strand, within Pf1N1B4_3314at 3704.174 kb on - strandat 3704.310 kb on + strandat 3704.310 kb on + strandat 3704.319 kb on + strandat 3704.515 kb on + strandat 3704.515 kb on + strandat 3704.516 kb on - strandat 3704.656 kb on + strand, within Pf1N1B4_3316at 3704.924 kb on - strand, within Pf1N1B4_3316at 3705.100 kb on + strand, within Pf1N1B4_3316at 3705.100 kb on + strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.170 kb on - strandat 3705.194 kb on - strandat 3705.311 kb on + strandat 3705.395 kb on + strand, within Pf1N1B4_3317at 3705.409 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.563 kb on + strand, within Pf1N1B4_3317at 3705.563 kb on + strand, within Pf1N1B4_3317at 3705.564 kb on - strand, within Pf1N1B4_3317at 3705.564 kb on - strand, within Pf1N1B4_3317at 3705.564 kb on - strandat 3705.958 kb on + strand, within Pf1N1B4_3317at 3705.959 kb on - strand, within Pf1N1B4_3317at 3705.959 kb on - strand, within Pf1N1B4_3317

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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3,703,569 + Pf1N1B4_3314 0.43 +3.9
3,703,569 + Pf1N1B4_3314 0.43 -0.5
3,703,569 + Pf1N1B4_3314 0.43 -0.5
3,703,569 + +0.2
3,703,569 + Pf1N1B4_3314 0.43 +0.2
3,703,570 - Pf1N1B4_3314 0.43 -1.8
3,703,626 + Pf1N1B4_3314 0.48 -1.8
3,703,923 + Pf1N1B4_3314 0.71 +1.2
3,704,001 - Pf1N1B4_3314 0.77 -2.0
3,704,145 + Pf1N1B4_3314 0.88 +1.3
3,704,145 + Pf1N1B4_3314 0.88 -0.4
3,704,145 + Pf1N1B4_3314 0.88 -1.2
3,704,146 - Pf1N1B4_3314 0.88 -1.2
3,704,146 - Pf1N1B4_3314 0.88 +1.6
3,704,146 - Pf1N1B4_3314 0.88 +1.1
3,704,146 - Pf1N1B4_3314 0.88 +1.3
3,704,146 - Pf1N1B4_3314 0.88 +1.5
3,704,174 - +0.6
3,704,310 + -0.2
3,704,310 + +1.2
3,704,319 + -1.8
3,704,515 + +0.0
3,704,515 + -0.2
3,704,516 - +2.1
3,704,656 + Pf1N1B4_3316 0.20 -0.8
3,704,924 - Pf1N1B4_3316 0.60 -1.8
3,705,100 + Pf1N1B4_3316 0.86 -0.5
3,705,100 + Pf1N1B4_3316 0.86 -0.9
3,705,101 - Pf1N1B4_3316 0.86 +0.1
3,705,101 - Pf1N1B4_3316 0.86 -1.4
3,705,101 - Pf1N1B4_3316 0.86 -0.8
3,705,101 - Pf1N1B4_3316 0.86 -0.4
3,705,101 - Pf1N1B4_3316 0.86 -1.2
3,705,101 - Pf1N1B4_3316 0.86 +0.8
3,705,101 - Pf1N1B4_3316 0.86 -2.7
3,705,101 - Pf1N1B4_3316 0.86 +0.2
3,705,170 - -0.5
3,705,194 - +0.3
3,705,311 + +0.0
3,705,395 + Pf1N1B4_3317 0.15 -0.2
3,705,409 + Pf1N1B4_3317 0.16 -0.0
3,705,551 + Pf1N1B4_3317 0.26 +0.5
3,705,551 + Pf1N1B4_3317 0.26 -0.8
3,705,551 + Pf1N1B4_3317 0.26 -1.1
3,705,551 + Pf1N1B4_3317 0.26 -0.5
3,705,551 + Pf1N1B4_3317 0.26 +0.5
3,705,552 - Pf1N1B4_3317 0.26 +0.7
3,705,552 - Pf1N1B4_3317 0.26 -0.1
3,705,552 - Pf1N1B4_3317 0.26 +0.5
3,705,552 - Pf1N1B4_3317 0.26 -1.0
3,705,563 + Pf1N1B4_3317 0.27 -0.6
3,705,563 + Pf1N1B4_3317 0.27 +0.4
3,705,564 - Pf1N1B4_3317 0.27 +0.8
3,705,564 - Pf1N1B4_3317 0.27 +1.2
3,705,564 - +0.8
3,705,958 + Pf1N1B4_3317 0.55 -0.1
3,705,959 - Pf1N1B4_3317 0.56 -2.4
3,705,959 - Pf1N1B4_3317 0.56 +0.5

Or see this region's nucleotide sequence