Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3314 and Pf1N1B4_3315 are separated by 18 nucleotides Pf1N1B4_3315 and Pf1N1B4_3316 are separated by 50 nucleotides Pf1N1B4_3316 and Pf1N1B4_3317 overlap by 8 nucleotides
Pf1N1B4_3314 - Tricarboxylate porin OpdH, at 3,703,010 to 3,704,302
_3314
Pf1N1B4_3315 - hypothetical protein, at 3,704,321 to 3,704,473
_3315
Pf1N1B4_3316 - Tricarboxylate transport transcriptional regulator TctD, at 3,704,524 to 3,705,195
_3316
Pf1N1B4_3317 - Tricarboxylate transport sensor protein TctE, at 3,705,188 to 3,706,576
_3317
Position (kb)
3704
3705
3706 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.570 kb on - strand, within Pf1N1B4_3314 at 3703.626 kb on + strand, within Pf1N1B4_3314 at 3703.923 kb on + strand, within Pf1N1B4_3314 at 3704.001 kb on - strand, within Pf1N1B4_3314 at 3704.145 kb on + strand, within Pf1N1B4_3314 at 3704.145 kb on + strand, within Pf1N1B4_3314 at 3704.145 kb on + strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.174 kb on - strand at 3704.310 kb on + strand at 3704.310 kb on + strand at 3704.319 kb on + strand at 3704.515 kb on + strand at 3704.515 kb on + strand at 3704.516 kb on - strand at 3704.656 kb on + strand, within Pf1N1B4_3316 at 3704.924 kb on - strand, within Pf1N1B4_3316 at 3705.100 kb on + strand, within Pf1N1B4_3316 at 3705.100 kb on + strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.170 kb on - strand at 3705.194 kb on - strand at 3705.311 kb on + strand at 3705.395 kb on + strand, within Pf1N1B4_3317 at 3705.409 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.563 kb on + strand, within Pf1N1B4_3317 at 3705.563 kb on + strand, within Pf1N1B4_3317 at 3705.564 kb on - strand, within Pf1N1B4_3317 at 3705.564 kb on - strand, within Pf1N1B4_3317 at 3705.564 kb on - strand at 3705.958 kb on + strand, within Pf1N1B4_3317 at 3705.959 kb on - strand, within Pf1N1B4_3317 at 3705.959 kb on - strand, within Pf1N1B4_3317
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,703,569 + Pf1N1B4_3314 0.43 +3.9 3,703,569 + Pf1N1B4_3314 0.43 -0.5 3,703,569 + Pf1N1B4_3314 0.43 -0.5 3,703,569 + +0.2 3,703,569 + Pf1N1B4_3314 0.43 +0.2 3,703,570 - Pf1N1B4_3314 0.43 -1.8 3,703,626 + Pf1N1B4_3314 0.48 -1.8 3,703,923 + Pf1N1B4_3314 0.71 +1.2 3,704,001 - Pf1N1B4_3314 0.77 -2.0 3,704,145 + Pf1N1B4_3314 0.88 +1.3 3,704,145 + Pf1N1B4_3314 0.88 -0.4 3,704,145 + Pf1N1B4_3314 0.88 -1.2 3,704,146 - Pf1N1B4_3314 0.88 -1.2 3,704,146 - Pf1N1B4_3314 0.88 +1.6 3,704,146 - Pf1N1B4_3314 0.88 +1.1 3,704,146 - Pf1N1B4_3314 0.88 +1.3 3,704,146 - Pf1N1B4_3314 0.88 +1.5 3,704,174 - +0.6 3,704,310 + -0.2 3,704,310 + +1.2 3,704,319 + -1.8 3,704,515 + +0.0 3,704,515 + -0.2 3,704,516 - +2.1 3,704,656 + Pf1N1B4_3316 0.20 -0.8 3,704,924 - Pf1N1B4_3316 0.60 -1.8 3,705,100 + Pf1N1B4_3316 0.86 -0.5 3,705,100 + Pf1N1B4_3316 0.86 -0.9 3,705,101 - Pf1N1B4_3316 0.86 +0.1 3,705,101 - Pf1N1B4_3316 0.86 -1.4 3,705,101 - Pf1N1B4_3316 0.86 -0.8 3,705,101 - Pf1N1B4_3316 0.86 -0.4 3,705,101 - Pf1N1B4_3316 0.86 -1.2 3,705,101 - Pf1N1B4_3316 0.86 +0.8 3,705,101 - Pf1N1B4_3316 0.86 -2.7 3,705,101 - Pf1N1B4_3316 0.86 +0.2 3,705,170 - -0.5 3,705,194 - +0.3 3,705,311 + +0.0 3,705,395 + Pf1N1B4_3317 0.15 -0.2 3,705,409 + Pf1N1B4_3317 0.16 -0.0 3,705,551 + Pf1N1B4_3317 0.26 +0.5 3,705,551 + Pf1N1B4_3317 0.26 -0.8 3,705,551 + Pf1N1B4_3317 0.26 -1.1 3,705,551 + Pf1N1B4_3317 0.26 -0.5 3,705,551 + Pf1N1B4_3317 0.26 +0.5 3,705,552 - Pf1N1B4_3317 0.26 +0.7 3,705,552 - Pf1N1B4_3317 0.26 -0.1 3,705,552 - Pf1N1B4_3317 0.26 +0.5 3,705,552 - Pf1N1B4_3317 0.26 -1.0 3,705,563 + Pf1N1B4_3317 0.27 -0.6 3,705,563 + Pf1N1B4_3317 0.27 +0.4 3,705,564 - Pf1N1B4_3317 0.27 +0.8 3,705,564 - Pf1N1B4_3317 0.27 +1.2 3,705,564 - +0.8 3,705,958 + Pf1N1B4_3317 0.55 -0.1 3,705,959 - Pf1N1B4_3317 0.56 -2.4 3,705,959 - Pf1N1B4_3317 0.56 +0.5
Or see this region's nucleotide sequence