Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3244 and Pf1N1B4_3245 are separated by 146 nucleotides Pf1N1B4_3245 and Pf1N1B4_3246 overlap by 1 nucleotides
Pf1N1B4_3244 - NADH oxidase, at 3,630,193 to 3,632,148
_3244
Pf1N1B4_3245 - RND efflux system, outer membrane lipoprotein, NodT family, at 3,632,295 to 3,633,755
_3245
Pf1N1B4_3246 - RND efflux system, inner membrane transporter CmeB, at 3,633,755 to 3,636,916
_3246
Position (kb)
3632
3633
3634 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3631.390 kb on + strand, within Pf1N1B4_3244 at 3631.423 kb on + strand, within Pf1N1B4_3244 at 3631.424 kb on - strand, within Pf1N1B4_3244 at 3631.424 kb on - strand, within Pf1N1B4_3244 at 3631.424 kb on - strand, within Pf1N1B4_3244 at 3631.501 kb on + strand, within Pf1N1B4_3244 at 3631.501 kb on + strand, within Pf1N1B4_3244 at 3631.501 kb on + strand, within Pf1N1B4_3244 at 3631.502 kb on - strand, within Pf1N1B4_3244 at 3631.657 kb on + strand, within Pf1N1B4_3244 at 3631.658 kb on - strand, within Pf1N1B4_3244 at 3631.658 kb on - strand, within Pf1N1B4_3244 at 3631.660 kb on + strand, within Pf1N1B4_3244 at 3631.660 kb on + strand at 3631.660 kb on + strand, within Pf1N1B4_3244 at 3631.660 kb on + strand, within Pf1N1B4_3244 at 3631.660 kb on + strand, within Pf1N1B4_3244 at 3631.660 kb on + strand, within Pf1N1B4_3244 at 3631.660 kb on + strand, within Pf1N1B4_3244 at 3631.661 kb on - strand at 3631.661 kb on - strand, within Pf1N1B4_3244 at 3631.661 kb on - strand, within Pf1N1B4_3244 at 3631.759 kb on + strand, within Pf1N1B4_3244 at 3631.759 kb on + strand, within Pf1N1B4_3244 at 3631.760 kb on - strand, within Pf1N1B4_3244 at 3631.760 kb on - strand, within Pf1N1B4_3244 at 3631.838 kb on + strand, within Pf1N1B4_3244 at 3631.839 kb on - strand, within Pf1N1B4_3244 at 3631.839 kb on - strand, within Pf1N1B4_3244 at 3631.923 kb on + strand, within Pf1N1B4_3244 at 3631.923 kb on + strand, within Pf1N1B4_3244 at 3631.923 kb on + strand, within Pf1N1B4_3244 at 3631.924 kb on - strand, within Pf1N1B4_3244 at 3631.955 kb on - strand at 3632.190 kb on + strand at 3632.190 kb on + strand at 3632.190 kb on + strand at 3632.190 kb on + strand at 3632.193 kb on + strand at 3632.193 kb on + strand at 3632.193 kb on + strand at 3632.193 kb on + strand at 3632.381 kb on - strand at 3632.522 kb on - strand, within Pf1N1B4_3245 at 3633.143 kb on - strand, within Pf1N1B4_3245 at 3633.215 kb on - strand, within Pf1N1B4_3245 at 3633.281 kb on - strand, within Pf1N1B4_3245 at 3633.281 kb on - strand, within Pf1N1B4_3245 at 3633.338 kb on - strand, within Pf1N1B4_3245 at 3633.770 kb on - strand at 3633.770 kb on - strand at 3633.770 kb on - strand at 3634.037 kb on - strand at 3634.132 kb on - strand, within Pf1N1B4_3246 at 3634.318 kb on - strand, within Pf1N1B4_3246 at 3634.442 kb on + strand, within Pf1N1B4_3246 at 3634.594 kb on - strand, within Pf1N1B4_3246 at 3634.594 kb on - strand, within Pf1N1B4_3246 at 3634.635 kb on + strand, within Pf1N1B4_3246 at 3634.636 kb on - strand, within Pf1N1B4_3246 at 3634.640 kb on - strand at 3634.640 kb on - strand, within Pf1N1B4_3246
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,631,390 + Pf1N1B4_3244 0.61 -1.8 3,631,423 + Pf1N1B4_3244 0.63 +1.0 3,631,424 - Pf1N1B4_3244 0.63 +0.2 3,631,424 - Pf1N1B4_3244 0.63 -0.5 3,631,424 - Pf1N1B4_3244 0.63 -0.5 3,631,501 + Pf1N1B4_3244 0.67 -0.6 3,631,501 + Pf1N1B4_3244 0.67 +0.2 3,631,501 + Pf1N1B4_3244 0.67 +1.3 3,631,502 - Pf1N1B4_3244 0.67 -2.3 3,631,657 + Pf1N1B4_3244 0.75 +1.2 3,631,658 - Pf1N1B4_3244 0.75 -0.2 3,631,658 - Pf1N1B4_3244 0.75 +0.4 3,631,660 + Pf1N1B4_3244 0.75 +0.8 3,631,660 + -0.2 3,631,660 + Pf1N1B4_3244 0.75 +0.4 3,631,660 + Pf1N1B4_3244 0.75 +1.1 3,631,660 + Pf1N1B4_3244 0.75 -0.6 3,631,660 + Pf1N1B4_3244 0.75 -0.2 3,631,660 + Pf1N1B4_3244 0.75 -0.8 3,631,661 - +2.4 3,631,661 - Pf1N1B4_3244 0.75 +1.6 3,631,661 - Pf1N1B4_3244 0.75 -1.5 3,631,759 + Pf1N1B4_3244 0.80 +1.8 3,631,759 + Pf1N1B4_3244 0.80 -1.2 3,631,760 - Pf1N1B4_3244 0.80 -1.2 3,631,760 - Pf1N1B4_3244 0.80 -0.0 3,631,838 + Pf1N1B4_3244 0.84 +1.1 3,631,839 - Pf1N1B4_3244 0.84 +0.3 3,631,839 - Pf1N1B4_3244 0.84 +0.8 3,631,923 + Pf1N1B4_3244 0.88 -1.5 3,631,923 + Pf1N1B4_3244 0.88 +2.4 3,631,923 + Pf1N1B4_3244 0.88 -0.5 3,631,924 - Pf1N1B4_3244 0.88 -0.8 3,631,955 - -1.0 3,632,190 + -1.2 3,632,190 + +0.4 3,632,190 + +0.2 3,632,190 + -2.2 3,632,193 + +0.1 3,632,193 + +0.7 3,632,193 + -1.2 3,632,193 + +0.1 3,632,381 - -1.2 3,632,522 - Pf1N1B4_3245 0.16 -1.8 3,633,143 - Pf1N1B4_3245 0.58 -2.3 3,633,215 - Pf1N1B4_3245 0.63 -1.5 3,633,281 - Pf1N1B4_3245 0.67 +0.8 3,633,281 - Pf1N1B4_3245 0.67 -2.8 3,633,338 - Pf1N1B4_3245 0.71 -0.5 3,633,770 - -1.2 3,633,770 - -2.5 3,633,770 - -0.8 3,634,037 - +1.6 3,634,132 - Pf1N1B4_3246 0.12 +0.1 3,634,318 - Pf1N1B4_3246 0.18 -1.3 3,634,442 + Pf1N1B4_3246 0.22 -3.6 3,634,594 - Pf1N1B4_3246 0.27 +2.8 3,634,594 - Pf1N1B4_3246 0.27 -0.8 3,634,635 + Pf1N1B4_3246 0.28 -2.0 3,634,636 - Pf1N1B4_3246 0.28 -2.0 3,634,640 - +0.5 3,634,640 - Pf1N1B4_3246 0.28 -1.8
Or see this region's nucleotide sequence