Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3189 and Pf1N1B4_3190 are separated by 114 nucleotides Pf1N1B4_3190 and Pf1N1B4_3191 are separated by 148 nucleotides Pf1N1B4_3191 and Pf1N1B4_3192 overlap by 8 nucleotides
Pf1N1B4_3189 - Outer membrane protein A precursor, at 3,571,112 to 3,571,807
_3189
Pf1N1B4_3190 - Outer membrane protein A precursor, at 3,571,922 to 3,572,611
_3190
Pf1N1B4_3191 - FIG00953505: hypothetical protein, at 3,572,760 to 3,573,038
_3191
Pf1N1B4_3192 - Collagen pro alpha-chain precursor, at 3,573,031 to 3,573,789
_3192
Position (kb)
3571
3572
3573 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3570.938 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.962 kb on - strand at 3571.085 kb on - strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.135 kb on - strand at 3571.135 kb on - strand at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.382 kb on - strand, within Pf1N1B4_3189 at 3571.382 kb on - strand at 3571.382 kb on - strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand at 3571.533 kb on - strand, within Pf1N1B4_3189 at 3571.534 kb on - strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.626 kb on - strand at 3571.626 kb on - strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.671 kb on - strand, within Pf1N1B4_3189 at 3571.671 kb on - strand at 3571.671 kb on - strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.805 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.852 kb on + strand at 3571.852 kb on + strand at 3571.852 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.910 kb on - strand at 3571.910 kb on - strand at 3571.910 kb on - strand at 3571.910 kb on - strand at 3571.910 kb on - strand at 3571.910 kb on - strand at 3571.910 kb on - strand at 3572.063 kb on - strand at 3572.063 kb on - strand, within Pf1N1B4_3190 at 3572.182 kb on + strand, within Pf1N1B4_3190 at 3572.306 kb on + strand, within Pf1N1B4_3190 at 3572.306 kb on + strand, within Pf1N1B4_3190 at 3572.306 kb on + strand, within Pf1N1B4_3190 at 3572.306 kb on + strand, within Pf1N1B4_3190 at 3572.307 kb on - strand, within Pf1N1B4_3190 at 3572.307 kb on - strand, within Pf1N1B4_3190 at 3572.307 kb on - strand, within Pf1N1B4_3190 at 3572.307 kb on - strand, within Pf1N1B4_3190 at 3572.392 kb on + strand, within Pf1N1B4_3190 at 3572.392 kb on + strand, within Pf1N1B4_3190 at 3572.392 kb on + strand, within Pf1N1B4_3190 at 3572.392 kb on + strand, within Pf1N1B4_3190 at 3572.393 kb on - strand, within Pf1N1B4_3190 at 3572.393 kb on - strand, within Pf1N1B4_3190 at 3572.432 kb on + strand, within Pf1N1B4_3190 at 3572.433 kb on - strand, within Pf1N1B4_3190 at 3572.433 kb on - strand, within Pf1N1B4_3190 at 3572.433 kb on - strand, within Pf1N1B4_3190 at 3572.433 kb on - strand, within Pf1N1B4_3190 at 3572.478 kb on - strand, within Pf1N1B4_3190 at 3572.618 kb on + strand at 3572.618 kb on + strand at 3572.618 kb on + strand at 3572.618 kb on + strand at 3572.618 kb on + strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.619 kb on - strand at 3572.621 kb on + strand at 3572.621 kb on + strand at 3572.621 kb on + strand at 3572.622 kb on - strand at 3572.622 kb on - strand at 3572.622 kb on - strand at 3572.713 kb on + strand at 3572.714 kb on - strand at 3572.714 kb on - strand at 3572.721 kb on + strand at 3572.740 kb on - strand at 3572.740 kb on - strand at 3572.740 kb on - strand at 3572.759 kb on + strand at 3572.759 kb on + strand at 3572.759 kb on + strand at 3572.759 kb on + strand at 3572.759 kb on + strand at 3572.759 kb on + strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.760 kb on - strand at 3572.833 kb on + strand, within Pf1N1B4_3191 at 3572.834 kb on - strand, within Pf1N1B4_3191 at 3572.834 kb on - strand, within Pf1N1B4_3191 at 3573.036 kb on + strand at 3573.037 kb on - strand at 3573.037 kb on - strand at 3573.047 kb on + strand at 3573.048 kb on - strand at 3573.048 kb on - strand at 3573.060 kb on + strand at 3573.060 kb on + strand at 3573.060 kb on + strand at 3573.135 kb on + strand at 3573.135 kb on + strand at 3573.135 kb on + strand, within Pf1N1B4_3192 at 3573.136 kb on - strand, within Pf1N1B4_3192 at 3573.343 kb on + strand, within Pf1N1B4_3192 at 3573.343 kb on + strand, within Pf1N1B4_3192 at 3573.343 kb on + strand, within Pf1N1B4_3192 at 3573.343 kb on + strand, within Pf1N1B4_3192 at 3573.343 kb on + strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.344 kb on - strand, within Pf1N1B4_3192 at 3573.489 kb on + strand, within Pf1N1B4_3192 at 3573.489 kb on + strand, within Pf1N1B4_3192 at 3573.490 kb on - strand, within Pf1N1B4_3192 at 3573.490 kb on - strand, within Pf1N1B4_3192 at 3573.525 kb on + strand, within Pf1N1B4_3192 at 3573.590 kb on - strand, within Pf1N1B4_3192
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,570,938 + +2.2 3,570,961 + -1.2 3,570,961 + +2.9 3,570,961 + +0.7 3,570,961 + +0.9 3,570,961 + -1.1 3,570,962 - -1.9 3,571,085 - -0.1 3,571,109 + +1.1 3,571,109 + -0.5 3,571,109 + -0.7 3,571,109 + +0.2 3,571,109 + -0.5 3,571,109 + -1.7 3,571,110 - +0.4 3,571,110 - +0.3 3,571,110 - +1.6 3,571,110 - -1.1 3,571,110 - +0.1 3,571,110 - +1.3 3,571,110 - -0.1 3,571,110 - -0.5 3,571,119 + -0.5 3,571,119 + -1.7 3,571,119 + +0.4 3,571,119 + +2.0 3,571,119 + +0.3 3,571,119 + +0.7 3,571,119 + +0.9 3,571,119 + +1.1 3,571,135 - +0.4 3,571,135 - +0.9 3,571,381 + Pf1N1B4_3189 0.39 +2.2 3,571,381 + Pf1N1B4_3189 0.39 -1.5 3,571,381 + Pf1N1B4_3189 0.39 +0.7 3,571,382 - Pf1N1B4_3189 0.39 +0.7 3,571,382 - -1.5 3,571,382 - Pf1N1B4_3189 0.39 -1.1 3,571,533 + Pf1N1B4_3189 0.60 -1.0 3,571,533 + Pf1N1B4_3189 0.60 +0.9 3,571,533 + Pf1N1B4_3189 0.60 -1.7 3,571,533 + Pf1N1B4_3189 0.60 -0.9 3,571,533 + Pf1N1B4_3189 0.60 +0.9 3,571,533 + +0.3 3,571,533 - Pf1N1B4_3189 0.60 +1.1 3,571,534 - Pf1N1B4_3189 0.61 -0.1 3,571,625 + Pf1N1B4_3189 0.74 +0.1 3,571,625 + Pf1N1B4_3189 0.74 -0.1 3,571,625 + Pf1N1B4_3189 0.74 +1.7 3,571,625 + Pf1N1B4_3189 0.74 +2.0 3,571,625 + Pf1N1B4_3189 0.74 +0.3 3,571,625 + Pf1N1B4_3189 0.74 +1.2 3,571,626 - +1.3 3,571,626 - Pf1N1B4_3189 0.74 +0.0 3,571,670 + Pf1N1B4_3189 0.80 +1.0 3,571,670 + Pf1N1B4_3189 0.80 +1.7 3,571,670 + Pf1N1B4_3189 0.80 -1.6 3,571,671 - Pf1N1B4_3189 0.80 +0.5 3,571,671 - -0.1 3,571,671 - Pf1N1B4_3189 0.80 -0.6 3,571,699 + Pf1N1B4_3189 0.84 -0.5 3,571,699 + Pf1N1B4_3189 0.84 +2.3 3,571,699 + Pf1N1B4_3189 0.84 +0.5 3,571,700 - Pf1N1B4_3189 0.84 -0.7 3,571,700 - Pf1N1B4_3189 0.84 -1.5 3,571,700 - Pf1N1B4_3189 0.84 +0.3 3,571,700 - Pf1N1B4_3189 0.84 +0.5 3,571,805 + -0.4 3,571,830 + -1.7 3,571,830 + +1.5 3,571,830 + -0.1 3,571,830 + +1.5 3,571,830 + +0.3 3,571,830 + -0.1 3,571,830 + -1.5 3,571,831 - -0.5 3,571,831 - +0.5 3,571,831 - +1.3 3,571,831 - +0.5 3,571,852 + +0.1 3,571,852 + -1.8 3,571,852 + +0.6 3,571,909 + +0.2 3,571,909 + -0.7 3,571,909 + +0.5 3,571,909 + -1.0 3,571,909 + +0.9 3,571,909 + -2.6 3,571,909 + -0.9 3,571,910 - -1.7 3,571,910 - +0.3 3,571,910 - +0.4 3,571,910 - +1.4 3,571,910 - -0.6 3,571,910 - -1.1 3,571,910 - -0.1 3,572,063 - -0.7 3,572,063 - Pf1N1B4_3190 0.20 -0.5 3,572,182 + Pf1N1B4_3190 0.38 -0.7 3,572,306 + Pf1N1B4_3190 0.56 -2.5 3,572,306 + Pf1N1B4_3190 0.56 +0.4 3,572,306 + Pf1N1B4_3190 0.56 -1.9 3,572,306 + Pf1N1B4_3190 0.56 -1.1 3,572,307 - Pf1N1B4_3190 0.56 -0.1 3,572,307 - Pf1N1B4_3190 0.56 -1.0 3,572,307 - Pf1N1B4_3190 0.56 +0.5 3,572,307 - Pf1N1B4_3190 0.56 +0.3 3,572,392 + Pf1N1B4_3190 0.68 -0.5 3,572,392 + Pf1N1B4_3190 0.68 +0.3 3,572,392 + Pf1N1B4_3190 0.68 +1.9 3,572,392 + Pf1N1B4_3190 0.68 +1.5 3,572,393 - Pf1N1B4_3190 0.68 -1.9 3,572,393 - Pf1N1B4_3190 0.68 +0.9 3,572,432 + Pf1N1B4_3190 0.74 -1.9 3,572,433 - Pf1N1B4_3190 0.74 -1.5 3,572,433 - Pf1N1B4_3190 0.74 -0.7 3,572,433 - Pf1N1B4_3190 0.74 -1.7 3,572,433 - Pf1N1B4_3190 0.74 -1.1 3,572,478 - Pf1N1B4_3190 0.81 -1.2 3,572,618 + -1.3 3,572,618 + -0.3 3,572,618 + +0.5 3,572,618 + -0.3 3,572,618 + +0.0 3,572,619 - +0.5 3,572,619 - -2.1 3,572,619 - -0.7 3,572,619 - +1.2 3,572,619 - -1.1 3,572,619 - -0.2 3,572,619 - -0.1 3,572,619 - -1.5 3,572,619 - +0.0 3,572,619 - -1.1 3,572,621 + -1.5 3,572,621 + +0.5 3,572,621 + +0.9 3,572,622 - -0.9 3,572,622 - +1.8 3,572,622 - -0.1 3,572,713 + -0.8 3,572,714 - +0.6 3,572,714 - +0.9 3,572,721 + +1.5 3,572,740 - -0.3 3,572,740 - -0.1 3,572,740 - +1.5 3,572,759 + +1.6 3,572,759 + -0.6 3,572,759 + +1.6 3,572,759 + -0.1 3,572,759 + +0.2 3,572,759 + -0.1 3,572,760 - -2.1 3,572,760 - +0.9 3,572,760 - -0.1 3,572,760 - +1.2 3,572,760 - -1.5 3,572,760 - -1.3 3,572,760 - +0.9 3,572,760 - +0.5 3,572,760 - -1.5 3,572,760 - -1.0 3,572,760 - +1.1 3,572,760 - +1.0 3,572,833 + Pf1N1B4_3191 0.26 -0.1 3,572,834 - Pf1N1B4_3191 0.27 -1.5 3,572,834 - Pf1N1B4_3191 0.27 +0.8 3,573,036 + +1.6 3,573,037 - +0.3 3,573,037 - +0.9 3,573,047 + -0.7 3,573,048 - +0.9 3,573,048 - -0.5 3,573,060 + -0.7 3,573,060 + -0.1 3,573,060 + +0.2 3,573,135 + -1.1 3,573,135 + +0.3 3,573,135 + Pf1N1B4_3192 0.14 -0.1 3,573,136 - Pf1N1B4_3192 0.14 +0.3 3,573,343 + Pf1N1B4_3192 0.41 -0.7 3,573,343 + Pf1N1B4_3192 0.41 -0.6 3,573,343 + Pf1N1B4_3192 0.41 +0.9 3,573,343 + Pf1N1B4_3192 0.41 +0.7 3,573,343 + Pf1N1B4_3192 0.41 +0.0 3,573,344 - Pf1N1B4_3192 0.41 +0.1 3,573,344 - Pf1N1B4_3192 0.41 -0.1 3,573,344 - Pf1N1B4_3192 0.41 +0.1 3,573,344 - Pf1N1B4_3192 0.41 -0.0 3,573,344 - Pf1N1B4_3192 0.41 +0.4 3,573,344 - Pf1N1B4_3192 0.41 +0.2 3,573,344 - Pf1N1B4_3192 0.41 +1.4 3,573,489 + Pf1N1B4_3192 0.60 -0.9 3,573,489 + Pf1N1B4_3192 0.60 +1.1 3,573,490 - Pf1N1B4_3192 0.60 +0.9 3,573,490 - Pf1N1B4_3192 0.60 +0.3 3,573,525 + Pf1N1B4_3192 0.65 -0.9 3,573,590 - Pf1N1B4_3192 0.74 +0.9
Or see this region's nucleotide sequence