Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3118 and Pf1N1B4_3119 are separated by 521 nucleotides Pf1N1B4_3119 and Pf1N1B4_3120 are separated by 29 nucleotides Pf1N1B4_3120 and Pf1N1B4_3121 overlap by 35 nucleotides Pf1N1B4_3121 and Pf1N1B4_3122 overlap by 4 nucleotides Pf1N1B4_3122 and Pf1N1B4_3123 overlap by 1 nucleotides
Pf1N1B4_3118 - Transcriptional regulator, Cro/CI family, at 3,506,083 to 3,506,826
_3118
Pf1N1B4_3119 - Holin, at 3,507,348 to 3,507,695
_3119
Pf1N1B4_3120 - hypothetical protein, at 3,507,725 to 3,507,859
_3120
Pf1N1B4_3121 - FIG111678: IS, phage, Tn; Transposon-related functions, at 3,507,825 to 3,508,409
_3121
Pf1N1B4_3122 - Mu-like prophage FluMu protein GP38, at 3,508,406 to 3,508,588
_3122
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Position (kb)
3507
3508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3506.825 kb on - strand at 3506.843 kb on + strand at 3506.843 kb on + strand at 3506.843 kb on + strand at 3506.844 kb on - strand at 3506.844 kb on - strand at 3506.844 kb on - strand at 3507.030 kb on + strand at 3507.030 kb on + strand at 3507.031 kb on - strand at 3507.105 kb on + strand at 3507.105 kb on + strand at 3507.106 kb on - strand at 3507.106 kb on - strand at 3507.106 kb on - strand at 3507.213 kb on - strand at 3507.245 kb on + strand at 3507.305 kb on + strand at 3507.306 kb on - strand at 3507.541 kb on - strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.625 kb on - strand at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.825 kb on - strand at 3507.913 kb on + strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.948 kb on + strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.137 kb on + strand, within Pf1N1B4_3121 at 3508.152 kb on + strand, within Pf1N1B4_3121 at 3508.153 kb on - strand, within Pf1N1B4_3121 at 3508.399 kb on + strand at 3508.399 kb on + strand at 3508.587 kb on - strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.640 kb on - strand at 3508.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,506,825 - +0.5 3,506,843 + -1.1 3,506,843 + +1.1 3,506,843 + -0.6 3,506,844 - +1.1 3,506,844 - +0.0 3,506,844 - +0.3 3,507,030 + -0.7 3,507,030 + +0.9 3,507,031 - -0.9 3,507,105 + +0.6 3,507,105 + -1.9 3,507,106 - +0.6 3,507,106 - -0.4 3,507,106 - +2.1 3,507,213 - +1.3 3,507,245 + +0.3 3,507,305 + +0.3 3,507,306 - +0.8 3,507,541 - Pf1N1B4_3119 0.55 -1.3 3,507,624 + Pf1N1B4_3119 0.79 -1.1 3,507,624 + Pf1N1B4_3119 0.79 +0.3 3,507,625 - +0.3 3,507,824 + Pf1N1B4_3120 0.73 +0.6 3,507,824 + Pf1N1B4_3120 0.73 -0.1 3,507,824 + Pf1N1B4_3120 0.73 -0.1 3,507,825 - +2.5 3,507,913 + Pf1N1B4_3121 0.15 +1.4 3,507,914 - Pf1N1B4_3121 0.15 -1.3 3,507,914 - Pf1N1B4_3121 0.15 -0.6 3,507,914 - Pf1N1B4_3121 0.15 +1.9 3,507,948 + Pf1N1B4_3121 0.21 +0.5 3,507,949 - Pf1N1B4_3121 0.21 +1.2 3,507,949 - Pf1N1B4_3121 0.21 -0.4 3,507,949 - Pf1N1B4_3121 0.21 -0.7 3,507,949 - Pf1N1B4_3121 0.21 -0.6 3,507,949 - Pf1N1B4_3121 0.21 -0.7 3,508,100 - Pf1N1B4_3121 0.47 +0.9 3,508,100 - Pf1N1B4_3121 0.47 -0.2 3,508,137 + Pf1N1B4_3121 0.53 +0.3 3,508,152 + Pf1N1B4_3121 0.56 -0.1 3,508,153 - Pf1N1B4_3121 0.56 +0.0 3,508,399 + -0.3 3,508,399 + +0.9 3,508,587 - +0.5 3,508,639 + -2.1 3,508,639 + +0.4 3,508,639 + +0.5 3,508,640 - +0.1 3,508,640 - +1.1
Or see this region's nucleotide sequence