Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_274 and Pf1N1B4_275 are separated by 69 nucleotides Pf1N1B4_275 and Pf1N1B4_276 are separated by 350 nucleotides Pf1N1B4_276 and Pf1N1B4_277 are separated by 32 nucleotides
Pf1N1B4_274 - Transcriptional regulator, LysR family, at 333,631 to 334,623
_274
Pf1N1B4_275 - Organic hydroperoxide resistance transcriptional regulator, at 334,693 to 335,154
_275
Pf1N1B4_276 - Organic hydroperoxide resistance protein, at 335,505 to 335,924
_276
Pf1N1B4_277 - Lipase, at 335,957 to 336,973
_277
Position (kb) 334
335
336 Strain fitness (log2 ratio) -2
-1
0
1
2 at 333.933 kb on + strand at 333.934 kb on - strand, within Pf1N1B4_274 at 334.109 kb on + strand, within Pf1N1B4_274 at 334.109 kb on + strand, within Pf1N1B4_274 at 334.109 kb on + strand, within Pf1N1B4_274 at 334.110 kb on - strand, within Pf1N1B4_274 at 334.112 kb on + strand, within Pf1N1B4_274 at 334.112 kb on + strand, within Pf1N1B4_274 at 334.112 kb on + strand, within Pf1N1B4_274 at 334.112 kb on + strand, within Pf1N1B4_274 at 334.112 kb on + strand, within Pf1N1B4_274 at 334.113 kb on - strand, within Pf1N1B4_274 at 334.113 kb on - strand, within Pf1N1B4_274 at 334.430 kb on + strand, within Pf1N1B4_274 at 334.430 kb on + strand, within Pf1N1B4_274 at 334.430 kb on + strand, within Pf1N1B4_274 at 334.430 kb on + strand, within Pf1N1B4_274 at 334.431 kb on - strand, within Pf1N1B4_274 at 334.431 kb on - strand at 334.431 kb on - strand at 334.431 kb on - strand, within Pf1N1B4_274 at 334.433 kb on + strand, within Pf1N1B4_274 at 334.498 kb on + strand at 334.498 kb on + strand, within Pf1N1B4_274 at 334.498 kb on + strand, within Pf1N1B4_274 at 334.499 kb on - strand, within Pf1N1B4_274 at 334.499 kb on - strand, within Pf1N1B4_274 at 334.499 kb on - strand, within Pf1N1B4_274 at 334.499 kb on - strand, within Pf1N1B4_274 at 334.552 kb on + strand at 334.622 kb on - strand at 334.795 kb on + strand, within Pf1N1B4_275 at 334.796 kb on - strand, within Pf1N1B4_275 at 334.796 kb on - strand, within Pf1N1B4_275 at 334.828 kb on + strand, within Pf1N1B4_275 at 334.994 kb on + strand, within Pf1N1B4_275 at 334.994 kb on + strand, within Pf1N1B4_275 at 334.995 kb on - strand at 335.130 kb on + strand at 335.131 kb on - strand at 335.131 kb on - strand at 335.145 kb on + strand at 335.348 kb on + strand at 335.348 kb on + strand at 335.348 kb on + strand at 335.349 kb on - strand at 335.349 kb on - strand at 335.356 kb on + strand at 335.357 kb on - strand at 335.421 kb on - strand at 335.518 kb on - strand at 335.845 kb on + strand, within Pf1N1B4_276 at 335.845 kb on + strand, within Pf1N1B4_276 at 335.846 kb on - strand, within Pf1N1B4_276 at 335.846 kb on - strand, within Pf1N1B4_276 at 335.846 kb on - strand, within Pf1N1B4_276 at 335.922 kb on + strand at 335.922 kb on + strand at 335.923 kb on - strand at 335.923 kb on - strand at 335.923 kb on - strand at 335.923 kb on - strand at 335.956 kb on + strand at 335.956 kb on + strand at 335.956 kb on + strand at 335.957 kb on - strand at 335.957 kb on - strand at 335.957 kb on - strand at 335.957 kb on - strand at 336.063 kb on + strand, within Pf1N1B4_277 at 336.063 kb on + strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand at 336.065 kb on - strand, within Pf1N1B4_277 at 336.147 kb on + strand, within Pf1N1B4_277
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 333,933 + +1.1 333,934 - Pf1N1B4_274 0.31 +0.5 334,109 + Pf1N1B4_274 0.48 -0.3 334,109 + Pf1N1B4_274 0.48 +0.9 334,109 + Pf1N1B4_274 0.48 -0.8 334,110 - Pf1N1B4_274 0.48 +0.0 334,112 + Pf1N1B4_274 0.48 -1.7 334,112 + Pf1N1B4_274 0.48 -0.8 334,112 + Pf1N1B4_274 0.48 +1.1 334,112 + Pf1N1B4_274 0.48 +0.7 334,112 + Pf1N1B4_274 0.48 +1.1 334,113 - Pf1N1B4_274 0.49 +0.0 334,113 - Pf1N1B4_274 0.49 -0.5 334,430 + Pf1N1B4_274 0.80 +2.2 334,430 + Pf1N1B4_274 0.80 +0.3 334,430 + Pf1N1B4_274 0.80 +0.8 334,430 + Pf1N1B4_274 0.80 +2.1 334,431 - Pf1N1B4_274 0.81 +0.2 334,431 - +1.0 334,431 - +1.7 334,431 - Pf1N1B4_274 0.81 +1.1 334,433 + Pf1N1B4_274 0.81 +1.7 334,498 + +1.1 334,498 + Pf1N1B4_274 0.87 +0.3 334,498 + Pf1N1B4_274 0.87 -0.3 334,499 - Pf1N1B4_274 0.87 +0.5 334,499 - Pf1N1B4_274 0.87 +1.2 334,499 - Pf1N1B4_274 0.87 +1.3 334,499 - Pf1N1B4_274 0.87 +1.2 334,552 + +1.1 334,622 - +1.4 334,795 + Pf1N1B4_275 0.22 +1.1 334,796 - Pf1N1B4_275 0.22 -1.9 334,796 - Pf1N1B4_275 0.22 -1.3 334,828 + Pf1N1B4_275 0.29 -1.0 334,994 + Pf1N1B4_275 0.65 -0.5 334,994 + Pf1N1B4_275 0.65 -1.2 334,995 - -1.3 335,130 + -0.7 335,131 - -1.0 335,131 - -0.3 335,145 + -1.3 335,348 + -0.5 335,348 + -0.5 335,348 + -2.3 335,349 - -1.9 335,349 - +0.4 335,356 + -1.8 335,357 - -1.3 335,421 - -0.5 335,518 - -0.2 335,845 + Pf1N1B4_276 0.81 +0.0 335,845 + Pf1N1B4_276 0.81 +0.3 335,846 - Pf1N1B4_276 0.81 -0.5 335,846 - Pf1N1B4_276 0.81 +0.6 335,846 - Pf1N1B4_276 0.81 +1.8 335,922 + +0.7 335,922 + -0.3 335,923 - -0.7 335,923 - +0.9 335,923 - +0.0 335,923 - +1.5 335,956 + -1.7 335,956 + +0.9 335,956 + +0.7 335,957 - +0.7 335,957 - -0.2 335,957 - -1.7 335,957 - -0.4 336,063 + Pf1N1B4_277 0.10 -1.5 336,063 + Pf1N1B4_277 0.10 -0.0 336,064 - Pf1N1B4_277 0.11 +0.7 336,064 - Pf1N1B4_277 0.11 -1.1 336,064 - Pf1N1B4_277 0.11 -0.7 336,064 - Pf1N1B4_277 0.11 -1.1 336,064 - Pf1N1B4_277 0.11 +1.7 336,064 - +2.3 336,065 - Pf1N1B4_277 0.11 +0.2 336,147 + Pf1N1B4_277 0.19 -0.7
Or see this region's nucleotide sequence