Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2431 and Pf1N1B4_2432 are separated by 224 nucleotides Pf1N1B4_2432 and Pf1N1B4_2433 are separated by 137 nucleotides Pf1N1B4_2433 and Pf1N1B4_2434 are separated by 32 nucleotides Pf1N1B4_2434 and Pf1N1B4_2435 are separated by 40 nucleotides
Pf1N1B4_2431 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,756,236 to 2,757,180
_2431
Pf1N1B4_2432 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,757,405 to 2,758,349
_2432
Pf1N1B4_2433 - FIG00953108: hypothetical protein, at 2,758,487 to 2,758,903
_2433
Pf1N1B4_2434 - putative DNA binding protein, at 2,758,936 to 2,759,202
_2434
Pf1N1B4_2435 - hypothetical protein, at 2,759,243 to 2,759,395
_2435
Position (kb)
2757
2758
2759 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2756.411 kb on + strand, within Pf1N1B4_2431 at 2756.411 kb on + strand, within Pf1N1B4_2431 at 2756.412 kb on - strand, within Pf1N1B4_2431 at 2756.534 kb on + strand, within Pf1N1B4_2431 at 2756.534 kb on + strand, within Pf1N1B4_2431 at 2756.535 kb on - strand, within Pf1N1B4_2431 at 2756.535 kb on - strand, within Pf1N1B4_2431 at 2756.535 kb on - strand, within Pf1N1B4_2431 at 2756.535 kb on - strand, within Pf1N1B4_2431 at 2756.535 kb on - strand, within Pf1N1B4_2431 at 2756.739 kb on - strand, within Pf1N1B4_2431 at 2756.810 kb on + strand, within Pf1N1B4_2431 at 2756.810 kb on + strand, within Pf1N1B4_2431 at 2756.811 kb on - strand, within Pf1N1B4_2431 at 2757.006 kb on - strand, within Pf1N1B4_2431 at 2757.006 kb on - strand, within Pf1N1B4_2431 at 2757.245 kb on + strand at 2757.389 kb on + strand at 2757.420 kb on + strand at 2757.420 kb on + strand at 2757.421 kb on - strand at 2757.510 kb on + strand, within Pf1N1B4_2432 at 2757.619 kb on + strand, within Pf1N1B4_2432 at 2757.811 kb on - strand, within Pf1N1B4_2432 at 2758.088 kb on + strand, within Pf1N1B4_2432 at 2758.132 kb on + strand, within Pf1N1B4_2432 at 2758.132 kb on + strand, within Pf1N1B4_2432 at 2758.180 kb on - strand, within Pf1N1B4_2432 at 2758.279 kb on - strand at 2758.348 kb on - strand at 2758.488 kb on + strand at 2758.489 kb on - strand at 2758.521 kb on + strand at 2758.522 kb on - strand at 2758.522 kb on - strand at 2758.522 kb on - strand at 2758.537 kb on - strand, within Pf1N1B4_2433 at 2758.537 kb on - strand, within Pf1N1B4_2433 at 2758.660 kb on + strand, within Pf1N1B4_2433 at 2758.660 kb on + strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.938 kb on - strand at 2758.938 kb on - strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2759.076 kb on + strand, within Pf1N1B4_2434 at 2759.077 kb on - strand, within Pf1N1B4_2434 at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.247 kb on + strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.281 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.329 kb on + strand, within Pf1N1B4_2435
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,756,411 + Pf1N1B4_2431 0.19 +0.6 2,756,411 + Pf1N1B4_2431 0.19 +0.0 2,756,412 - Pf1N1B4_2431 0.19 +0.8 2,756,534 + Pf1N1B4_2431 0.32 -0.9 2,756,534 + Pf1N1B4_2431 0.32 -1.9 2,756,535 - Pf1N1B4_2431 0.32 +1.1 2,756,535 - Pf1N1B4_2431 0.32 -0.9 2,756,535 - Pf1N1B4_2431 0.32 +0.5 2,756,535 - Pf1N1B4_2431 0.32 +0.3 2,756,535 - Pf1N1B4_2431 0.32 +1.4 2,756,739 - Pf1N1B4_2431 0.53 -1.5 2,756,810 + Pf1N1B4_2431 0.61 +0.4 2,756,810 + Pf1N1B4_2431 0.61 +0.5 2,756,811 - Pf1N1B4_2431 0.61 +0.5 2,757,006 - Pf1N1B4_2431 0.81 +0.6 2,757,006 - Pf1N1B4_2431 0.81 -0.1 2,757,245 + -1.1 2,757,389 + -1.0 2,757,420 + +1.2 2,757,420 + -1.2 2,757,421 - +1.4 2,757,510 + Pf1N1B4_2432 0.11 -1.7 2,757,619 + Pf1N1B4_2432 0.23 -0.4 2,757,811 - Pf1N1B4_2432 0.43 +0.6 2,758,088 + Pf1N1B4_2432 0.72 -1.5 2,758,132 + Pf1N1B4_2432 0.77 -0.9 2,758,132 + Pf1N1B4_2432 0.77 -0.9 2,758,180 - Pf1N1B4_2432 0.82 -0.6 2,758,279 - +0.8 2,758,348 - +0.5 2,758,488 + -0.1 2,758,489 - -2.8 2,758,521 + +1.1 2,758,522 - +1.4 2,758,522 - -0.2 2,758,522 - -0.3 2,758,537 - Pf1N1B4_2433 0.12 +0.5 2,758,537 - Pf1N1B4_2433 0.12 +0.1 2,758,660 + Pf1N1B4_2433 0.41 +0.4 2,758,660 + Pf1N1B4_2433 0.41 +0.1 2,758,661 - Pf1N1B4_2433 0.42 +1.5 2,758,661 - Pf1N1B4_2433 0.42 +1.1 2,758,661 - Pf1N1B4_2433 0.42 +0.9 2,758,661 - Pf1N1B4_2433 0.42 -0.4 2,758,937 + -0.0 2,758,937 + +0.4 2,758,937 + -1.1 2,758,938 - +1.5 2,758,938 - +0.5 2,758,945 + +0.3 2,758,945 + -0.1 2,758,945 + -0.2 2,759,076 + Pf1N1B4_2434 0.52 +0.2 2,759,077 - Pf1N1B4_2434 0.53 -0.2 2,759,180 + +0.7 2,759,180 + +0.5 2,759,180 + -2.5 2,759,181 - +0.7 2,759,181 - +0.4 2,759,181 - -0.1 2,759,181 - -0.2 2,759,181 - -1.9 2,759,247 + +0.7 2,759,248 - -0.9 2,759,248 - -0.3 2,759,248 - -0.0 2,759,248 - -0.1 2,759,281 + Pf1N1B4_2435 0.25 -0.5 2,759,317 + Pf1N1B4_2435 0.48 -0.0 2,759,317 + Pf1N1B4_2435 0.48 -0.0 2,759,329 + Pf1N1B4_2435 0.56 -0.6
Or see this region's nucleotide sequence