Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1956 and Pf1N1B4_1957 overlap by 4 nucleotides Pf1N1B4_1957 and Pf1N1B4_1958 are separated by 435 nucleotides Pf1N1B4_1958 and Pf1N1B4_1959 are separated by 99 nucleotides
Pf1N1B4_1956 - Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17), at 2,237,024 to 2,237,890
_1956
Pf1N1B4_1957 - hypothetical protein, at 2,237,887 to 2,238,009
_1957
Pf1N1B4_1958 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 2,238,445 to 2,238,822
_1958
Pf1N1B4_1959 - Glycosyltransferase, at 2,238,922 to 2,240,052
_1959
Position (kb)
2238
2239 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2237.489 kb on + strand, within Pf1N1B4_1956 at 2237.489 kb on + strand, within Pf1N1B4_1956 at 2237.489 kb on + strand, within Pf1N1B4_1956 at 2237.489 kb on + strand, within Pf1N1B4_1956 at 2237.489 kb on + strand, within Pf1N1B4_1956 at 2237.489 kb on + strand, within Pf1N1B4_1956 at 2237.490 kb on - strand, within Pf1N1B4_1956 at 2237.490 kb on - strand at 2237.490 kb on - strand, within Pf1N1B4_1956 at 2237.490 kb on - strand, within Pf1N1B4_1956 at 2237.560 kb on - strand, within Pf1N1B4_1956 at 2237.579 kb on - strand, within Pf1N1B4_1956 at 2237.611 kb on + strand, within Pf1N1B4_1956 at 2237.611 kb on + strand, within Pf1N1B4_1956 at 2237.611 kb on + strand, within Pf1N1B4_1956 at 2237.611 kb on + strand, within Pf1N1B4_1956 at 2237.611 kb on + strand at 2237.611 kb on + strand, within Pf1N1B4_1956 at 2237.612 kb on - strand, within Pf1N1B4_1956 at 2237.612 kb on - strand, within Pf1N1B4_1956 at 2237.612 kb on - strand, within Pf1N1B4_1956 at 2237.908 kb on + strand, within Pf1N1B4_1957 at 2238.003 kb on + strand at 2238.003 kb on + strand at 2238.003 kb on + strand at 2238.004 kb on - strand at 2238.004 kb on - strand at 2238.004 kb on - strand at 2238.090 kb on + strand at 2238.091 kb on - strand at 2238.091 kb on - strand at 2238.156 kb on + strand at 2238.157 kb on - strand at 2238.161 kb on + strand at 2238.231 kb on + strand at 2238.284 kb on - strand at 2238.284 kb on - strand at 2238.284 kb on - strand at 2238.299 kb on + strand at 2238.299 kb on + strand at 2238.299 kb on + strand at 2238.300 kb on - strand at 2238.300 kb on - strand at 2238.300 kb on - strand at 2238.300 kb on - strand at 2238.300 kb on - strand at 2238.300 kb on - strand at 2238.550 kb on + strand, within Pf1N1B4_1958 at 2238.605 kb on - strand at 2238.605 kb on - strand, within Pf1N1B4_1958 at 2238.605 kb on - strand, within Pf1N1B4_1958 at 2238.605 kb on - strand, within Pf1N1B4_1958 at 2238.825 kb on + strand at 2238.825 kb on + strand at 2238.825 kb on + strand at 2238.826 kb on - strand at 2238.826 kb on - strand at 2238.826 kb on - strand at 2238.885 kb on + strand at 2238.886 kb on - strand at 2238.886 kb on - strand at 2238.886 kb on - strand at 2238.923 kb on + strand at 2238.923 kb on + strand at 2238.923 kb on + strand at 2238.929 kb on + strand at 2238.929 kb on + strand at 2238.929 kb on + strand at 2238.930 kb on - strand at 2238.952 kb on + strand at 2238.952 kb on + strand at 2238.952 kb on + strand at 2238.952 kb on + strand at 2238.953 kb on - strand at 2238.953 kb on - strand at 2238.962 kb on + strand at 2238.962 kb on + strand at 2238.962 kb on + strand at 2238.962 kb on + strand at 2238.962 kb on + strand at 2238.962 kb on + strand at 2238.962 kb on + strand at 2238.963 kb on - strand at 2238.963 kb on - strand at 2238.963 kb on - strand at 2238.963 kb on - strand at 2238.963 kb on - strand at 2239.145 kb on + strand, within Pf1N1B4_1959 at 2239.145 kb on + strand, within Pf1N1B4_1959 at 2239.146 kb on - strand, within Pf1N1B4_1959 at 2239.146 kb on - strand, within Pf1N1B4_1959 at 2239.146 kb on - strand, within Pf1N1B4_1959 at 2239.146 kb on - strand, within Pf1N1B4_1959 at 2239.146 kb on - strand, within Pf1N1B4_1959 at 2239.146 kb on - strand, within Pf1N1B4_1959 at 2239.190 kb on + strand, within Pf1N1B4_1959 at 2239.191 kb on - strand, within Pf1N1B4_1959 at 2239.249 kb on + strand, within Pf1N1B4_1959 at 2239.249 kb on + strand at 2239.249 kb on + strand, within Pf1N1B4_1959 at 2239.249 kb on + strand, within Pf1N1B4_1959 at 2239.250 kb on - strand, within Pf1N1B4_1959 at 2239.250 kb on - strand, within Pf1N1B4_1959 at 2239.250 kb on - strand, within Pf1N1B4_1959 at 2239.250 kb on - strand at 2239.259 kb on + strand, within Pf1N1B4_1959 at 2239.317 kb on + strand, within Pf1N1B4_1959 at 2239.318 kb on - strand, within Pf1N1B4_1959 at 2239.395 kb on - strand at 2239.439 kb on + strand, within Pf1N1B4_1959 at 2239.439 kb on + strand, within Pf1N1B4_1959 at 2239.439 kb on + strand, within Pf1N1B4_1959 at 2239.439 kb on + strand, within Pf1N1B4_1959 at 2239.439 kb on + strand, within Pf1N1B4_1959 at 2239.440 kb on - strand, within Pf1N1B4_1959 at 2239.440 kb on - strand, within Pf1N1B4_1959 at 2239.440 kb on - strand, within Pf1N1B4_1959 at 2239.440 kb on - strand, within Pf1N1B4_1959 at 2239.440 kb on - strand, within Pf1N1B4_1959 at 2239.440 kb on - strand, within Pf1N1B4_1959 at 2239.473 kb on + strand, within Pf1N1B4_1959 at 2239.473 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.604 kb on + strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.605 kb on - strand at 2239.605 kb on - strand, within Pf1N1B4_1959 at 2239.608 kb on + strand, within Pf1N1B4_1959 at 2239.608 kb on + strand, within Pf1N1B4_1959 at 2239.608 kb on + strand, within Pf1N1B4_1959 at 2239.608 kb on + strand, within Pf1N1B4_1959 at 2239.608 kb on + strand, within Pf1N1B4_1959 at 2239.609 kb on - strand, within Pf1N1B4_1959 at 2239.609 kb on - strand, within Pf1N1B4_1959 at 2239.609 kb on - strand, within Pf1N1B4_1959 at 2239.709 kb on + strand, within Pf1N1B4_1959 at 2239.709 kb on + strand, within Pf1N1B4_1959 at 2239.709 kb on + strand, within Pf1N1B4_1959 at 2239.710 kb on - strand, within Pf1N1B4_1959 at 2239.710 kb on - strand, within Pf1N1B4_1959 at 2239.717 kb on + strand, within Pf1N1B4_1959 at 2239.717 kb on + strand, within Pf1N1B4_1959 at 2239.717 kb on + strand, within Pf1N1B4_1959 at 2239.781 kb on + strand, within Pf1N1B4_1959 at 2239.781 kb on + strand, within Pf1N1B4_1959 at 2239.781 kb on + strand, within Pf1N1B4_1959 at 2239.782 kb on - strand, within Pf1N1B4_1959 at 2239.782 kb on - strand, within Pf1N1B4_1959
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,237,489 + Pf1N1B4_1956 0.54 -0.5 2,237,489 + Pf1N1B4_1956 0.54 -0.2 2,237,489 + Pf1N1B4_1956 0.54 -0.6 2,237,489 + Pf1N1B4_1956 0.54 +0.6 2,237,489 + Pf1N1B4_1956 0.54 +0.3 2,237,489 + Pf1N1B4_1956 0.54 -1.2 2,237,490 - Pf1N1B4_1956 0.54 -0.8 2,237,490 - -0.3 2,237,490 - Pf1N1B4_1956 0.54 +0.0 2,237,490 - Pf1N1B4_1956 0.54 -0.7 2,237,560 - Pf1N1B4_1956 0.62 +1.0 2,237,579 - Pf1N1B4_1956 0.64 -1.4 2,237,611 + Pf1N1B4_1956 0.68 +0.3 2,237,611 + Pf1N1B4_1956 0.68 +1.8 2,237,611 + Pf1N1B4_1956 0.68 +0.0 2,237,611 + Pf1N1B4_1956 0.68 +1.0 2,237,611 + +1.3 2,237,611 + Pf1N1B4_1956 0.68 -0.9 2,237,612 - Pf1N1B4_1956 0.68 +0.1 2,237,612 - Pf1N1B4_1956 0.68 -1.5 2,237,612 - Pf1N1B4_1956 0.68 +0.8 2,237,908 + Pf1N1B4_1957 0.17 +0.3 2,238,003 + +0.4 2,238,003 + +0.3 2,238,003 + -1.1 2,238,004 - +0.6 2,238,004 - +0.3 2,238,004 - +1.3 2,238,090 + +1.6 2,238,091 - +1.0 2,238,091 - +1.0 2,238,156 + -0.1 2,238,157 - -0.7 2,238,161 + +0.1 2,238,231 + +0.1 2,238,284 - -0.7 2,238,284 - +0.6 2,238,284 - -0.7 2,238,299 + +1.2 2,238,299 + -0.2 2,238,299 + +1.3 2,238,300 - -1.1 2,238,300 - +1.5 2,238,300 - +1.0 2,238,300 - +1.1 2,238,300 - -0.5 2,238,300 - -0.1 2,238,550 + Pf1N1B4_1958 0.28 -0.2 2,238,605 - +1.3 2,238,605 - Pf1N1B4_1958 0.42 -1.7 2,238,605 - Pf1N1B4_1958 0.42 -0.1 2,238,605 - Pf1N1B4_1958 0.42 -0.2 2,238,825 + +0.6 2,238,825 + +1.7 2,238,825 + +0.3 2,238,826 - +0.6 2,238,826 - +0.8 2,238,826 - -0.5 2,238,885 + -1.3 2,238,886 - -0.1 2,238,886 - +0.7 2,238,886 - -0.7 2,238,923 + +0.4 2,238,923 + +0.5 2,238,923 + +0.3 2,238,929 + -0.5 2,238,929 + +1.3 2,238,929 + -2.2 2,238,930 - +1.6 2,238,952 + -1.8 2,238,952 + +0.9 2,238,952 + -0.7 2,238,952 + -2.3 2,238,953 - -0.7 2,238,953 - -0.3 2,238,962 + +0.9 2,238,962 + +1.3 2,238,962 + +0.1 2,238,962 + +2.3 2,238,962 + +2.0 2,238,962 + -0.6 2,238,962 + -0.2 2,238,963 - +0.5 2,238,963 - +0.5 2,238,963 - +1.9 2,238,963 - +0.7 2,238,963 - -0.5 2,239,145 + Pf1N1B4_1959 0.20 +0.1 2,239,145 + Pf1N1B4_1959 0.20 -0.0 2,239,146 - Pf1N1B4_1959 0.20 -0.5 2,239,146 - Pf1N1B4_1959 0.20 +0.8 2,239,146 - Pf1N1B4_1959 0.20 +0.0 2,239,146 - Pf1N1B4_1959 0.20 +0.1 2,239,146 - Pf1N1B4_1959 0.20 +1.6 2,239,146 - Pf1N1B4_1959 0.20 -1.1 2,239,190 + Pf1N1B4_1959 0.24 +0.7 2,239,191 - Pf1N1B4_1959 0.24 +0.8 2,239,249 + Pf1N1B4_1959 0.29 -1.9 2,239,249 + +1.3 2,239,249 + Pf1N1B4_1959 0.29 +0.3 2,239,249 + Pf1N1B4_1959 0.29 +1.3 2,239,250 - Pf1N1B4_1959 0.29 -0.5 2,239,250 - Pf1N1B4_1959 0.29 +1.3 2,239,250 - Pf1N1B4_1959 0.29 +0.7 2,239,250 - +2.3 2,239,259 + Pf1N1B4_1959 0.30 +0.6 2,239,317 + Pf1N1B4_1959 0.35 +0.6 2,239,318 - Pf1N1B4_1959 0.35 +1.3 2,239,395 - +0.3 2,239,439 + Pf1N1B4_1959 0.46 -0.3 2,239,439 + Pf1N1B4_1959 0.46 +0.6 2,239,439 + Pf1N1B4_1959 0.46 -0.7 2,239,439 + Pf1N1B4_1959 0.46 -0.7 2,239,439 + Pf1N1B4_1959 0.46 +0.1 2,239,440 - Pf1N1B4_1959 0.46 +0.4 2,239,440 - Pf1N1B4_1959 0.46 +0.9 2,239,440 - Pf1N1B4_1959 0.46 +0.7 2,239,440 - Pf1N1B4_1959 0.46 +0.7 2,239,440 - Pf1N1B4_1959 0.46 -0.5 2,239,440 - Pf1N1B4_1959 0.46 -0.3 2,239,473 + Pf1N1B4_1959 0.49 +0.6 2,239,473 + Pf1N1B4_1959 0.49 -0.3 2,239,604 + Pf1N1B4_1959 0.60 +0.7 2,239,604 + Pf1N1B4_1959 0.60 +1.2 2,239,604 + Pf1N1B4_1959 0.60 -0.8 2,239,604 + Pf1N1B4_1959 0.60 -1.0 2,239,604 + Pf1N1B4_1959 0.60 +1.3 2,239,604 + Pf1N1B4_1959 0.60 +0.5 2,239,604 + Pf1N1B4_1959 0.60 -1.5 2,239,604 + Pf1N1B4_1959 0.60 +0.2 2,239,604 + Pf1N1B4_1959 0.60 +1.1 2,239,604 + Pf1N1B4_1959 0.60 +0.9 2,239,604 + Pf1N1B4_1959 0.60 +0.3 2,239,604 + Pf1N1B4_1959 0.60 -1.1 2,239,604 + Pf1N1B4_1959 0.60 +0.6 2,239,604 + Pf1N1B4_1959 0.60 +1.1 2,239,605 - Pf1N1B4_1959 0.60 -0.4 2,239,605 - Pf1N1B4_1959 0.60 -0.6 2,239,605 - Pf1N1B4_1959 0.60 -0.9 2,239,605 - Pf1N1B4_1959 0.60 +1.6 2,239,605 - Pf1N1B4_1959 0.60 -2.3 2,239,605 - Pf1N1B4_1959 0.60 +0.5 2,239,605 - -0.1 2,239,605 - Pf1N1B4_1959 0.60 +0.0 2,239,605 - Pf1N1B4_1959 0.60 +1.4 2,239,605 - -0.3 2,239,605 - Pf1N1B4_1959 0.60 -0.5 2,239,608 + Pf1N1B4_1959 0.61 +0.7 2,239,608 + Pf1N1B4_1959 0.61 +0.7 2,239,608 + Pf1N1B4_1959 0.61 +1.4 2,239,608 + Pf1N1B4_1959 0.61 +0.4 2,239,608 + Pf1N1B4_1959 0.61 -1.7 2,239,609 - Pf1N1B4_1959 0.61 -0.1 2,239,609 - Pf1N1B4_1959 0.61 +0.8 2,239,609 - Pf1N1B4_1959 0.61 -0.7 2,239,709 + Pf1N1B4_1959 0.70 -0.1 2,239,709 + Pf1N1B4_1959 0.70 +0.6 2,239,709 + Pf1N1B4_1959 0.70 +0.3 2,239,710 - Pf1N1B4_1959 0.70 -1.5 2,239,710 - Pf1N1B4_1959 0.70 -0.2 2,239,717 + Pf1N1B4_1959 0.70 -0.3 2,239,717 + Pf1N1B4_1959 0.70 +1.6 2,239,717 + Pf1N1B4_1959 0.70 +1.1 2,239,781 + Pf1N1B4_1959 0.76 +1.0 2,239,781 + Pf1N1B4_1959 0.76 -0.9 2,239,781 + Pf1N1B4_1959 0.76 +1.3 2,239,782 - Pf1N1B4_1959 0.76 +0.4 2,239,782 - Pf1N1B4_1959 0.76 -2.4
Or see this region's nucleotide sequence