Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1905 and Pf1N1B4_1906 are separated by 106 nucleotides Pf1N1B4_1906 and Pf1N1B4_1907 are separated by 81 nucleotides
Pf1N1B4_1905 - Sulfate permease, at 2,178,981 to 2,180,549
_1905
Pf1N1B4_1906 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 2,180,656 to 2,181,477
_1906
Pf1N1B4_1907 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 2,181,559 to 2,182,473
_1907
Position (kb)
2180
2181
2182 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2179.944 kb on + strand, within Pf1N1B4_1905 at 2179.944 kb on + strand, within Pf1N1B4_1905 at 2179.944 kb on + strand, within Pf1N1B4_1905 at 2179.944 kb on + strand, within Pf1N1B4_1905 at 2179.944 kb on + strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2179.945 kb on - strand, within Pf1N1B4_1905 at 2180.023 kb on + strand, within Pf1N1B4_1905 at 2180.196 kb on + strand, within Pf1N1B4_1905 at 2180.196 kb on + strand, within Pf1N1B4_1905 at 2180.226 kb on + strand, within Pf1N1B4_1905 at 2180.226 kb on + strand, within Pf1N1B4_1905 at 2180.227 kb on - strand, within Pf1N1B4_1905 at 2180.325 kb on + strand, within Pf1N1B4_1905 at 2180.325 kb on + strand, within Pf1N1B4_1905 at 2180.325 kb on + strand, within Pf1N1B4_1905 at 2180.564 kb on + strand at 2180.564 kb on + strand at 2180.565 kb on - strand at 2180.649 kb on - strand at 2180.649 kb on - strand at 2180.726 kb on + strand at 2180.727 kb on - strand at 2180.831 kb on + strand, within Pf1N1B4_1906 at 2180.831 kb on + strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.844 kb on - strand, within Pf1N1B4_1906 at 2180.844 kb on - strand, within Pf1N1B4_1906 at 2180.890 kb on + strand, within Pf1N1B4_1906 at 2180.890 kb on + strand, within Pf1N1B4_1906 at 2180.891 kb on - strand, within Pf1N1B4_1906 at 2181.121 kb on - strand, within Pf1N1B4_1906 at 2181.380 kb on + strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.460 kb on + strand at 2181.461 kb on - strand at 2181.468 kb on - strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,179,944 + Pf1N1B4_1905 0.61 -1.2 2,179,944 + Pf1N1B4_1905 0.61 -1.1 2,179,944 + Pf1N1B4_1905 0.61 -0.3 2,179,944 + Pf1N1B4_1905 0.61 +0.0 2,179,944 + Pf1N1B4_1905 0.61 +1.3 2,179,945 - Pf1N1B4_1905 0.61 -1.1 2,179,945 - Pf1N1B4_1905 0.61 -0.3 2,179,945 - Pf1N1B4_1905 0.61 +0.5 2,179,945 - Pf1N1B4_1905 0.61 +0.6 2,179,945 - Pf1N1B4_1905 0.61 -0.4 2,179,945 - Pf1N1B4_1905 0.61 +0.5 2,179,945 - Pf1N1B4_1905 0.61 -0.6 2,179,945 - Pf1N1B4_1905 0.61 +0.1 2,179,945 - Pf1N1B4_1905 0.61 -1.7 2,180,023 + Pf1N1B4_1905 0.66 +1.7 2,180,196 + Pf1N1B4_1905 0.77 +2.0 2,180,196 + Pf1N1B4_1905 0.77 +0.3 2,180,226 + Pf1N1B4_1905 0.79 -2.7 2,180,226 + Pf1N1B4_1905 0.79 -0.1 2,180,227 - Pf1N1B4_1905 0.79 -1.9 2,180,325 + Pf1N1B4_1905 0.86 -0.2 2,180,325 + Pf1N1B4_1905 0.86 +0.4 2,180,325 + Pf1N1B4_1905 0.86 +1.6 2,180,564 + +0.5 2,180,564 + +0.1 2,180,565 - +0.3 2,180,649 - -0.2 2,180,649 - -1.1 2,180,726 + -1.3 2,180,727 - +2.3 2,180,831 + Pf1N1B4_1906 0.21 -0.3 2,180,831 + Pf1N1B4_1906 0.21 -1.7 2,180,832 - Pf1N1B4_1906 0.21 -1.6 2,180,832 - Pf1N1B4_1906 0.21 -0.1 2,180,832 - Pf1N1B4_1906 0.21 +1.7 2,180,844 - Pf1N1B4_1906 0.23 -1.2 2,180,844 - Pf1N1B4_1906 0.23 -1.6 2,180,890 + Pf1N1B4_1906 0.28 -0.3 2,180,890 + Pf1N1B4_1906 0.28 -1.9 2,180,891 - Pf1N1B4_1906 0.29 -1.1 2,181,121 - Pf1N1B4_1906 0.57 -0.9 2,181,380 + Pf1N1B4_1906 0.88 -1.6 2,181,381 - Pf1N1B4_1906 0.88 -1.1 2,181,381 - Pf1N1B4_1906 0.88 -1.3 2,181,381 - Pf1N1B4_1906 0.88 -0.7 2,181,460 + -0.3 2,181,461 - +1.8 2,181,468 - -1.2 2,181,479 + +0.8 2,181,479 + -0.1 2,181,479 + -0.3 2,181,479 + +0.1 2,181,480 - +1.3 2,181,480 - +0.3 2,181,480 - +0.0 2,181,480 - -0.6 2,181,480 - +1.3 2,181,480 - +1.3 2,181,480 - +0.8 2,181,480 - +0.3 2,181,480 - +0.7 2,181,480 - -0.1 2,181,480 - +0.1 2,181,480 - -0.5 2,181,495 - +1.3 2,181,495 - +0.7 2,181,495 - +0.3 2,181,495 - -1.3
Or see this region's nucleotide sequence