Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1836 and Pf1N1B4_1837 are separated by 238 nucleotides Pf1N1B4_1837 and Pf1N1B4_1838 are separated by 171 nucleotides Pf1N1B4_1838 and Pf1N1B4_1839 are separated by 6 nucleotides Pf1N1B4_1839 and Pf1N1B4_1840 are separated by 40 nucleotides
Pf1N1B4_1836 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,110,659 to 2,111,660
_1836
Pf1N1B4_1837 - Osmotically inducible protein C, at 2,111,899 to 2,112,330
_1837
Pf1N1B4_1838 - FIG00955509: hypothetical protein, at 2,112,502 to 2,112,735
_1838
Pf1N1B4_1839 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,112,742 to 2,113,161
_1839
Pf1N1B4_1840 - lipoprotein, putative, at 2,113,202 to 2,113,618
_1840
Position (kb)
2111
2112
2113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2111.002 kb on + strand, within Pf1N1B4_1836 at 2111.002 kb on + strand, within Pf1N1B4_1836 at 2111.002 kb on + strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.224 kb on + strand, within Pf1N1B4_1836 at 2111.461 kb on + strand, within Pf1N1B4_1836 at 2111.535 kb on - strand, within Pf1N1B4_1836 at 2111.536 kb on + strand, within Pf1N1B4_1836 at 2111.536 kb on + strand, within Pf1N1B4_1836 at 2111.537 kb on - strand, within Pf1N1B4_1836 at 2111.650 kb on - strand at 2111.650 kb on - strand at 2111.660 kb on + strand at 2111.660 kb on + strand at 2111.660 kb on + strand at 2111.682 kb on - strand at 2111.718 kb on + strand at 2111.718 kb on + strand at 2111.718 kb on + strand at 2111.718 kb on + strand at 2111.719 kb on - strand at 2111.719 kb on - strand at 2111.719 kb on - strand at 2111.719 kb on - strand at 2111.720 kb on + strand at 2111.720 kb on + strand at 2111.721 kb on - strand at 2111.721 kb on - strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.731 kb on + strand at 2111.732 kb on - strand at 2111.732 kb on - strand at 2111.732 kb on - strand at 2111.746 kb on + strand at 2111.747 kb on - strand at 2111.747 kb on - strand at 2111.748 kb on + strand at 2111.780 kb on + strand at 2111.828 kb on + strand at 2111.829 kb on - strand at 2111.905 kb on - strand at 2111.905 kb on - strand at 2112.118 kb on - strand, within Pf1N1B4_1837 at 2112.164 kb on + strand, within Pf1N1B4_1837 at 2112.191 kb on + strand, within Pf1N1B4_1837 at 2112.331 kb on + strand at 2112.343 kb on - strand at 2112.343 kb on - strand at 2112.343 kb on - strand at 2112.347 kb on + strand at 2112.348 kb on - strand at 2112.348 kb on - strand at 2112.446 kb on - strand at 2112.526 kb on - strand, within Pf1N1B4_1838 at 2112.628 kb on + strand, within Pf1N1B4_1838 at 2112.628 kb on + strand, within Pf1N1B4_1838 at 2112.628 kb on + strand, within Pf1N1B4_1838 at 2112.629 kb on - strand, within Pf1N1B4_1838 at 2112.715 kb on + strand at 2112.715 kb on + strand at 2112.716 kb on - strand at 2112.716 kb on - strand at 2112.716 kb on - strand at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.823 kb on + strand, within Pf1N1B4_1839 at 2112.823 kb on + strand, within Pf1N1B4_1839 at 2112.824 kb on - strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.845 kb on + strand, within Pf1N1B4_1839 at 2112.846 kb on - strand, within Pf1N1B4_1839 at 2112.872 kb on + strand, within Pf1N1B4_1839 at 2112.872 kb on + strand, within Pf1N1B4_1839 at 2112.872 kb on + strand, within Pf1N1B4_1839 at 2112.873 kb on - strand, within Pf1N1B4_1839 at 2112.873 kb on - strand, within Pf1N1B4_1839 at 2112.873 kb on - strand, within Pf1N1B4_1839 at 2112.888 kb on - strand, within Pf1N1B4_1839 at 2112.888 kb on - strand, within Pf1N1B4_1839 at 2112.955 kb on + strand, within Pf1N1B4_1839 at 2112.956 kb on - strand, within Pf1N1B4_1839 at 2112.956 kb on - strand, within Pf1N1B4_1839 at 2112.956 kb on - strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.992 kb on + strand, within Pf1N1B4_1839 at 2112.992 kb on + strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2113.183 kb on + strand at 2113.207 kb on + strand at 2113.207 kb on + strand at 2113.207 kb on + strand at 2113.207 kb on + strand at 2113.208 kb on + strand at 2113.208 kb on - strand at 2113.208 kb on - strand at 2113.260 kb on + strand, within Pf1N1B4_1840 at 2113.260 kb on + strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.288 kb on + strand, within Pf1N1B4_1840 at 2113.290 kb on + strand, within Pf1N1B4_1840 at 2113.290 kb on + strand, within Pf1N1B4_1840 at 2113.291 kb on - strand, within Pf1N1B4_1840 at 2113.291 kb on - strand, within Pf1N1B4_1840
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,111,002 + Pf1N1B4_1836 0.34 +0.2 2,111,002 + Pf1N1B4_1836 0.34 +0.5 2,111,002 + Pf1N1B4_1836 0.34 -1.1 2,111,003 - Pf1N1B4_1836 0.34 -0.2 2,111,003 - Pf1N1B4_1836 0.34 -0.2 2,111,003 - Pf1N1B4_1836 0.34 +0.5 2,111,003 - Pf1N1B4_1836 0.34 -1.1 2,111,003 - Pf1N1B4_1836 0.34 +0.4 2,111,224 + Pf1N1B4_1836 0.56 -0.3 2,111,461 + Pf1N1B4_1836 0.80 +0.7 2,111,535 - Pf1N1B4_1836 0.87 -0.8 2,111,536 + Pf1N1B4_1836 0.88 +0.9 2,111,536 + Pf1N1B4_1836 0.88 -0.6 2,111,537 - Pf1N1B4_1836 0.88 +0.4 2,111,650 - -0.8 2,111,650 - +1.3 2,111,660 + +0.3 2,111,660 + -0.5 2,111,660 + +2.6 2,111,682 - -1.1 2,111,718 + -0.6 2,111,718 + +0.2 2,111,718 + +0.2 2,111,718 + +1.7 2,111,719 - -0.6 2,111,719 - -1.0 2,111,719 - -0.3 2,111,719 - -1.1 2,111,720 + -0.5 2,111,720 + -0.3 2,111,721 - +0.9 2,111,721 - -0.2 2,111,731 + +1.2 2,111,731 + +1.5 2,111,731 + +1.0 2,111,731 + +0.2 2,111,731 + -1.8 2,111,731 + -0.9 2,111,731 + +0.9 2,111,731 + -1.1 2,111,731 + -0.8 2,111,732 - -0.3 2,111,732 - +0.9 2,111,732 - +1.2 2,111,746 + +0.8 2,111,747 - -0.2 2,111,747 - -0.3 2,111,748 + +0.9 2,111,780 + -2.1 2,111,828 + +0.6 2,111,829 - +0.6 2,111,905 - -1.1 2,111,905 - -0.3 2,112,118 - Pf1N1B4_1837 0.51 -1.3 2,112,164 + Pf1N1B4_1837 0.61 -0.9 2,112,191 + Pf1N1B4_1837 0.68 -0.1 2,112,331 + +0.2 2,112,343 - -0.3 2,112,343 - -1.0 2,112,343 - +0.8 2,112,347 + +2.2 2,112,348 - +0.2 2,112,348 - +1.2 2,112,446 - -0.3 2,112,526 - Pf1N1B4_1838 0.10 +0.4 2,112,628 + Pf1N1B4_1838 0.54 +0.7 2,112,628 + Pf1N1B4_1838 0.54 -0.3 2,112,628 + Pf1N1B4_1838 0.54 +0.2 2,112,629 - Pf1N1B4_1838 0.54 -2.6 2,112,715 + -1.8 2,112,715 + +0.2 2,112,716 - +0.2 2,112,716 - -1.3 2,112,716 - +0.6 2,112,797 + Pf1N1B4_1839 0.13 +0.3 2,112,797 + Pf1N1B4_1839 0.13 -0.7 2,112,797 + Pf1N1B4_1839 0.13 +0.1 2,112,797 + Pf1N1B4_1839 0.13 +0.2 2,112,798 - Pf1N1B4_1839 0.13 +0.2 2,112,798 - Pf1N1B4_1839 0.13 +0.2 2,112,798 - Pf1N1B4_1839 0.13 -0.9 2,112,798 - Pf1N1B4_1839 0.13 +1.1 2,112,823 + Pf1N1B4_1839 0.19 +0.4 2,112,823 + Pf1N1B4_1839 0.19 +1.2 2,112,824 - Pf1N1B4_1839 0.20 +0.1 2,112,825 + Pf1N1B4_1839 0.20 -1.1 2,112,825 + Pf1N1B4_1839 0.20 +1.2 2,112,825 + Pf1N1B4_1839 0.20 -0.8 2,112,825 + Pf1N1B4_1839 0.20 -1.3 2,112,825 + Pf1N1B4_1839 0.20 +0.5 2,112,825 + Pf1N1B4_1839 0.20 -1.2 2,112,825 + Pf1N1B4_1839 0.20 -0.5 2,112,825 + Pf1N1B4_1839 0.20 -0.8 2,112,826 - Pf1N1B4_1839 0.20 +0.2 2,112,826 - Pf1N1B4_1839 0.20 -1.6 2,112,826 - Pf1N1B4_1839 0.20 +0.2 2,112,826 - Pf1N1B4_1839 0.20 +1.0 2,112,826 - Pf1N1B4_1839 0.20 +1.2 2,112,845 + Pf1N1B4_1839 0.25 -1.1 2,112,846 - Pf1N1B4_1839 0.25 +0.5 2,112,872 + Pf1N1B4_1839 0.31 +0.9 2,112,872 + Pf1N1B4_1839 0.31 +1.6 2,112,872 + Pf1N1B4_1839 0.31 -0.8 2,112,873 - Pf1N1B4_1839 0.31 -0.1 2,112,873 - Pf1N1B4_1839 0.31 -2.7 2,112,873 - Pf1N1B4_1839 0.31 -0.2 2,112,888 - Pf1N1B4_1839 0.35 +0.7 2,112,888 - Pf1N1B4_1839 0.35 -0.5 2,112,955 + Pf1N1B4_1839 0.51 +1.2 2,112,956 - Pf1N1B4_1839 0.51 -1.9 2,112,956 - Pf1N1B4_1839 0.51 +1.0 2,112,956 - Pf1N1B4_1839 0.51 -0.8 2,112,961 + Pf1N1B4_1839 0.52 -1.1 2,112,961 + Pf1N1B4_1839 0.52 -0.1 2,112,961 + Pf1N1B4_1839 0.52 -0.6 2,112,961 + Pf1N1B4_1839 0.52 +0.8 2,112,961 + Pf1N1B4_1839 0.52 +0.1 2,112,961 + Pf1N1B4_1839 0.52 -1.3 2,112,962 - Pf1N1B4_1839 0.52 +1.2 2,112,962 - Pf1N1B4_1839 0.52 -0.3 2,112,962 - Pf1N1B4_1839 0.52 -1.6 2,112,962 - Pf1N1B4_1839 0.52 -0.6 2,112,962 - Pf1N1B4_1839 0.52 +0.2 2,112,962 - Pf1N1B4_1839 0.52 +0.2 2,112,992 + Pf1N1B4_1839 0.60 -0.2 2,112,992 + Pf1N1B4_1839 0.60 +0.8 2,112,993 - Pf1N1B4_1839 0.60 -0.1 2,112,993 - Pf1N1B4_1839 0.60 -0.6 2,112,993 - Pf1N1B4_1839 0.60 +0.7 2,112,993 - Pf1N1B4_1839 0.60 -0.3 2,113,183 + -0.5 2,113,207 + +0.7 2,113,207 + +0.2 2,113,207 + +1.5 2,113,207 + -2.1 2,113,208 + +0.6 2,113,208 - +1.0 2,113,208 - -0.1 2,113,260 + Pf1N1B4_1840 0.14 -0.1 2,113,260 + Pf1N1B4_1840 0.14 -0.2 2,113,261 - Pf1N1B4_1840 0.14 -1.6 2,113,261 - Pf1N1B4_1840 0.14 -0.8 2,113,261 - Pf1N1B4_1840 0.14 -0.5 2,113,261 - Pf1N1B4_1840 0.14 -1.8 2,113,261 - Pf1N1B4_1840 0.14 +0.8 2,113,261 - Pf1N1B4_1840 0.14 -1.6 2,113,288 + Pf1N1B4_1840 0.21 -0.6 2,113,290 + Pf1N1B4_1840 0.21 -1.3 2,113,290 + Pf1N1B4_1840 0.21 -0.3 2,113,291 - Pf1N1B4_1840 0.21 +0.2 2,113,291 - Pf1N1B4_1840 0.21 +1.2
Or see this region's nucleotide sequence