Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1824 and Pf1N1B4_1825 are separated by 221 nucleotides Pf1N1B4_1825 and Pf1N1B4_1826 are separated by 28 nucleotides Pf1N1B4_1826 and Pf1N1B4_1827 overlap by 4 nucleotides Pf1N1B4_1827 and Pf1N1B4_1828 are separated by 281 nucleotides
Pf1N1B4_1824 - Outer membrane receptor proteins, mostly Fe transport, at 2,101,810 to 2,102,127
_1824
Pf1N1B4_1825 - Holliday junction resolvasome, helicase subunit, at 2,102,349 to 2,102,666
_1825
Pf1N1B4_1826 - FIG00953248: hypothetical protein, at 2,102,695 to 2,103,015
_1826
Pf1N1B4_1827 - FIG00953482: hypothetical protein, at 2,103,012 to 2,104,973
_1827
Pf1N1B4_1828 - Mobile element protein, at 2,105,255 to 2,106,046
_1828
Position (kb)
2103
2104
2105 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2102.125 kb on + strand at 2102.125 kb on + strand at 2102.125 kb on + strand at 2102.125 kb on + strand at 2102.126 kb on - strand at 2102.126 kb on - strand at 2102.126 kb on - strand at 2102.126 kb on - strand at 2102.126 kb on - strand at 2102.126 kb on - strand at 2102.155 kb on + strand at 2102.155 kb on + strand at 2102.267 kb on + strand at 2102.267 kb on + strand at 2102.267 kb on + strand at 2102.267 kb on + strand at 2102.268 kb on - strand at 2102.268 kb on - strand at 2102.268 kb on - strand at 2102.334 kb on + strand at 2102.334 kb on + strand at 2102.334 kb on + strand at 2102.334 kb on + strand at 2102.334 kb on + strand at 2102.334 kb on + strand at 2102.334 kb on + strand at 2102.335 kb on - strand at 2102.366 kb on + strand at 2102.367 kb on - strand at 2102.505 kb on - strand, within Pf1N1B4_1825 at 2102.664 kb on + strand at 2102.664 kb on + strand at 2102.664 kb on + strand at 2102.664 kb on + strand at 2102.665 kb on - strand at 2102.665 kb on - strand at 2102.668 kb on + strand at 2102.700 kb on + strand at 2102.779 kb on - strand, within Pf1N1B4_1826 at 2103.088 kb on + strand at 2103.089 kb on - strand at 2103.459 kb on - strand, within Pf1N1B4_1827 at 2103.571 kb on - strand, within Pf1N1B4_1827 at 2103.634 kb on - strand, within Pf1N1B4_1827 at 2103.679 kb on - strand, within Pf1N1B4_1827 at 2103.679 kb on - strand at 2103.679 kb on - strand at 2103.679 kb on - strand, within Pf1N1B4_1827 at 2103.679 kb on - strand, within Pf1N1B4_1827 at 2103.699 kb on + strand, within Pf1N1B4_1827 at 2103.724 kb on - strand, within Pf1N1B4_1827 at 2103.729 kb on + strand, within Pf1N1B4_1827 at 2103.733 kb on - strand, within Pf1N1B4_1827 at 2103.871 kb on + strand, within Pf1N1B4_1827 at 2103.872 kb on - strand, within Pf1N1B4_1827 at 2104.024 kb on + strand, within Pf1N1B4_1827 at 2104.024 kb on + strand, within Pf1N1B4_1827 at 2104.024 kb on + strand, within Pf1N1B4_1827 at 2104.025 kb on - strand, within Pf1N1B4_1827 at 2104.025 kb on - strand, within Pf1N1B4_1827 at 2104.056 kb on - strand, within Pf1N1B4_1827 at 2104.060 kb on - strand, within Pf1N1B4_1827 at 2104.107 kb on + strand, within Pf1N1B4_1827 at 2104.108 kb on - strand, within Pf1N1B4_1827 at 2104.108 kb on - strand, within Pf1N1B4_1827 at 2105.005 kb on - strand at 2105.005 kb on - strand at 2105.005 kb on - strand at 2105.005 kb on - strand at 2105.005 kb on - strand at 2105.005 kb on - strand at 2105.005 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand at 2105.011 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,102,125 + -1.1 2,102,125 + -0.5 2,102,125 + -0.9 2,102,125 + +0.8 2,102,126 - +0.3 2,102,126 - +0.1 2,102,126 - +1.0 2,102,126 - +0.5 2,102,126 - -0.3 2,102,126 - -0.9 2,102,155 + +0.2 2,102,155 + +0.9 2,102,267 + +0.2 2,102,267 + +1.5 2,102,267 + +1.1 2,102,267 + -0.1 2,102,268 - +1.2 2,102,268 - +0.0 2,102,268 - +0.2 2,102,334 + +0.0 2,102,334 + +0.4 2,102,334 + -0.0 2,102,334 + -0.4 2,102,334 + +0.0 2,102,334 + +0.3 2,102,334 + +1.0 2,102,335 - +1.2 2,102,366 + -1.3 2,102,367 - +0.7 2,102,505 - Pf1N1B4_1825 0.49 -1.8 2,102,664 + +0.2 2,102,664 + -0.6 2,102,664 + -0.7 2,102,664 + -0.2 2,102,665 - +2.6 2,102,665 - -1.6 2,102,668 + +0.0 2,102,700 + -0.6 2,102,779 - Pf1N1B4_1826 0.26 -1.3 2,103,088 + +0.2 2,103,089 - +0.2 2,103,459 - Pf1N1B4_1827 0.23 +0.2 2,103,571 - Pf1N1B4_1827 0.28 +0.5 2,103,634 - Pf1N1B4_1827 0.32 -0.8 2,103,679 - Pf1N1B4_1827 0.34 +1.2 2,103,679 - +0.2 2,103,679 - -0.8 2,103,679 - Pf1N1B4_1827 0.34 -1.8 2,103,679 - Pf1N1B4_1827 0.34 -1.8 2,103,699 + Pf1N1B4_1827 0.35 -0.9 2,103,724 - Pf1N1B4_1827 0.36 +0.1 2,103,729 + Pf1N1B4_1827 0.37 -2.8 2,103,733 - Pf1N1B4_1827 0.37 +1.2 2,103,871 + Pf1N1B4_1827 0.44 -0.3 2,103,872 - Pf1N1B4_1827 0.44 +0.7 2,104,024 + Pf1N1B4_1827 0.52 -2.3 2,104,024 + Pf1N1B4_1827 0.52 -1.8 2,104,024 + Pf1N1B4_1827 0.52 -1.1 2,104,025 - Pf1N1B4_1827 0.52 +0.5 2,104,025 - Pf1N1B4_1827 0.52 -1.6 2,104,056 - Pf1N1B4_1827 0.53 +1.2 2,104,060 - Pf1N1B4_1827 0.53 -0.8 2,104,107 + Pf1N1B4_1827 0.56 +1.6 2,104,108 - Pf1N1B4_1827 0.56 -1.6 2,104,108 - Pf1N1B4_1827 0.56 -0.8 2,105,005 - +2.4 2,105,005 - -0.8 2,105,005 - -1.1 2,105,005 - +1.7 2,105,005 - +0.1 2,105,005 - +0.9 2,105,005 - -1.6 2,105,011 - -1.1 2,105,011 - +0.2 2,105,011 - +0.0 2,105,011 - -0.8 2,105,011 - +0.0 2,105,011 - +1.0 2,105,011 - +2.2 2,105,011 - -1.3 2,105,011 - -0.3 2,105,011 - +0.7 2,105,011 - +0.8 2,105,011 - +1.6 2,105,011 - -0.6 2,105,011 - -1.9
Or see this region's nucleotide sequence