Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1827

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1824 and Pf1N1B4_1825 are separated by 221 nucleotidesPf1N1B4_1825 and Pf1N1B4_1826 are separated by 28 nucleotidesPf1N1B4_1826 and Pf1N1B4_1827 overlap by 4 nucleotidesPf1N1B4_1827 and Pf1N1B4_1828 are separated by 281 nucleotides Pf1N1B4_1824 - Outer membrane receptor proteins, mostly Fe transport, at 2,101,810 to 2,102,127 _1824 Pf1N1B4_1825 - Holliday junction resolvasome, helicase subunit, at 2,102,349 to 2,102,666 _1825 Pf1N1B4_1826 - FIG00953248: hypothetical protein, at 2,102,695 to 2,103,015 _1826 Pf1N1B4_1827 - FIG00953482: hypothetical protein, at 2,103,012 to 2,104,973 _1827 Pf1N1B4_1828 - Mobile element protein, at 2,105,255 to 2,106,046 _1828 Position (kb) 2103 2104 2105Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2102.125 kb on + strandat 2102.125 kb on + strandat 2102.125 kb on + strandat 2102.125 kb on + strandat 2102.126 kb on - strandat 2102.126 kb on - strandat 2102.126 kb on - strandat 2102.126 kb on - strandat 2102.126 kb on - strandat 2102.126 kb on - strandat 2102.155 kb on + strandat 2102.155 kb on + strandat 2102.267 kb on + strandat 2102.267 kb on + strandat 2102.267 kb on + strandat 2102.267 kb on + strandat 2102.268 kb on - strandat 2102.268 kb on - strandat 2102.268 kb on - strandat 2102.334 kb on + strandat 2102.334 kb on + strandat 2102.334 kb on + strandat 2102.334 kb on + strandat 2102.334 kb on + strandat 2102.334 kb on + strandat 2102.334 kb on + strandat 2102.335 kb on - strandat 2102.366 kb on + strandat 2102.367 kb on - strandat 2102.505 kb on - strand, within Pf1N1B4_1825at 2102.664 kb on + strandat 2102.664 kb on + strandat 2102.664 kb on + strandat 2102.664 kb on + strandat 2102.665 kb on - strandat 2102.665 kb on - strandat 2102.668 kb on + strandat 2102.700 kb on + strandat 2102.779 kb on - strand, within Pf1N1B4_1826at 2103.088 kb on + strandat 2103.089 kb on - strandat 2103.459 kb on - strand, within Pf1N1B4_1827at 2103.571 kb on - strand, within Pf1N1B4_1827at 2103.634 kb on - strand, within Pf1N1B4_1827at 2103.679 kb on - strand, within Pf1N1B4_1827at 2103.679 kb on - strandat 2103.679 kb on - strandat 2103.679 kb on - strand, within Pf1N1B4_1827at 2103.679 kb on - strand, within Pf1N1B4_1827at 2103.699 kb on + strand, within Pf1N1B4_1827at 2103.724 kb on - strand, within Pf1N1B4_1827at 2103.729 kb on + strand, within Pf1N1B4_1827at 2103.733 kb on - strand, within Pf1N1B4_1827at 2103.871 kb on + strand, within Pf1N1B4_1827at 2103.872 kb on - strand, within Pf1N1B4_1827at 2104.024 kb on + strand, within Pf1N1B4_1827at 2104.024 kb on + strand, within Pf1N1B4_1827at 2104.024 kb on + strand, within Pf1N1B4_1827at 2104.025 kb on - strand, within Pf1N1B4_1827at 2104.025 kb on - strand, within Pf1N1B4_1827at 2104.056 kb on - strand, within Pf1N1B4_1827at 2104.060 kb on - strand, within Pf1N1B4_1827at 2104.107 kb on + strand, within Pf1N1B4_1827at 2104.108 kb on - strand, within Pf1N1B4_1827at 2104.108 kb on - strand, within Pf1N1B4_1827at 2105.005 kb on - strandat 2105.005 kb on - strandat 2105.005 kb on - strandat 2105.005 kb on - strandat 2105.005 kb on - strandat 2105.005 kb on - strandat 2105.005 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strandat 2105.011 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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2,102,125 + -1.1
2,102,125 + -0.5
2,102,125 + -0.9
2,102,125 + +0.8
2,102,126 - +0.3
2,102,126 - +0.1
2,102,126 - +1.0
2,102,126 - +0.5
2,102,126 - -0.3
2,102,126 - -0.9
2,102,155 + +0.2
2,102,155 + +0.9
2,102,267 + +0.2
2,102,267 + +1.5
2,102,267 + +1.1
2,102,267 + -0.1
2,102,268 - +1.2
2,102,268 - +0.0
2,102,268 - +0.2
2,102,334 + +0.0
2,102,334 + +0.4
2,102,334 + -0.0
2,102,334 + -0.4
2,102,334 + +0.0
2,102,334 + +0.3
2,102,334 + +1.0
2,102,335 - +1.2
2,102,366 + -1.3
2,102,367 - +0.7
2,102,505 - Pf1N1B4_1825 0.49 -1.8
2,102,664 + +0.2
2,102,664 + -0.6
2,102,664 + -0.7
2,102,664 + -0.2
2,102,665 - +2.6
2,102,665 - -1.6
2,102,668 + +0.0
2,102,700 + -0.6
2,102,779 - Pf1N1B4_1826 0.26 -1.3
2,103,088 + +0.2
2,103,089 - +0.2
2,103,459 - Pf1N1B4_1827 0.23 +0.2
2,103,571 - Pf1N1B4_1827 0.28 +0.5
2,103,634 - Pf1N1B4_1827 0.32 -0.8
2,103,679 - Pf1N1B4_1827 0.34 +1.2
2,103,679 - +0.2
2,103,679 - -0.8
2,103,679 - Pf1N1B4_1827 0.34 -1.8
2,103,679 - Pf1N1B4_1827 0.34 -1.8
2,103,699 + Pf1N1B4_1827 0.35 -0.9
2,103,724 - Pf1N1B4_1827 0.36 +0.1
2,103,729 + Pf1N1B4_1827 0.37 -2.8
2,103,733 - Pf1N1B4_1827 0.37 +1.2
2,103,871 + Pf1N1B4_1827 0.44 -0.3
2,103,872 - Pf1N1B4_1827 0.44 +0.7
2,104,024 + Pf1N1B4_1827 0.52 -2.3
2,104,024 + Pf1N1B4_1827 0.52 -1.8
2,104,024 + Pf1N1B4_1827 0.52 -1.1
2,104,025 - Pf1N1B4_1827 0.52 +0.5
2,104,025 - Pf1N1B4_1827 0.52 -1.6
2,104,056 - Pf1N1B4_1827 0.53 +1.2
2,104,060 - Pf1N1B4_1827 0.53 -0.8
2,104,107 + Pf1N1B4_1827 0.56 +1.6
2,104,108 - Pf1N1B4_1827 0.56 -1.6
2,104,108 - Pf1N1B4_1827 0.56 -0.8
2,105,005 - +2.4
2,105,005 - -0.8
2,105,005 - -1.1
2,105,005 - +1.7
2,105,005 - +0.1
2,105,005 - +0.9
2,105,005 - -1.6
2,105,011 - -1.1
2,105,011 - +0.2
2,105,011 - +0.0
2,105,011 - -0.8
2,105,011 - +0.0
2,105,011 - +1.0
2,105,011 - +2.2
2,105,011 - -1.3
2,105,011 - -0.3
2,105,011 - +0.7
2,105,011 - +0.8
2,105,011 - +1.6
2,105,011 - -0.6
2,105,011 - -1.9

Or see this region's nucleotide sequence