Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1786 and Pf1N1B4_1787 are separated by 228 nucleotides Pf1N1B4_1787 and Pf1N1B4_1788 are separated by 177 nucleotides Pf1N1B4_1788 and Pf1N1B4_1789 are separated by 69 nucleotides
Pf1N1B4_1786 - ATP-dependent DNA helicase, RecQ family, at 2,038,340 to 2,040,274
_1786
Pf1N1B4_1787 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,040,503 to 2,041,729
_1787
Pf1N1B4_1788 - putative 4-hydroxybenzoyl-CoA thioesterase, at 2,041,907 to 2,042,338
_1788
Pf1N1B4_1789 - FIG01270322: hypothetical protein, at 2,042,408 to 2,042,896
_1789
Position (kb)
2040
2041
2042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2039.546 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.577 kb on - strand at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.700 kb on - strand, within Pf1N1B4_1786 at 2039.708 kb on + strand, within Pf1N1B4_1786 at 2039.709 kb on - strand, within Pf1N1B4_1786 at 2039.747 kb on + strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.858 kb on + strand, within Pf1N1B4_1786 at 2039.859 kb on - strand, within Pf1N1B4_1786 at 2039.954 kb on + strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.175 kb on + strand at 2040.176 kb on - strand at 2040.225 kb on - strand at 2040.401 kb on - strand at 2040.401 kb on - strand at 2040.462 kb on - strand at 2040.462 kb on - strand at 2040.488 kb on - strand at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2041.021 kb on - strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.104 kb on - strand, within Pf1N1B4_1787 at 2041.155 kb on + strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.337 kb on + strand, within Pf1N1B4_1787 at 2041.338 kb on - strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.341 kb on - strand, within Pf1N1B4_1787 at 2041.547 kb on + strand, within Pf1N1B4_1787 at 2041.547 kb on + strand, within Pf1N1B4_1787 at 2041.548 kb on - strand, within Pf1N1B4_1787 at 2041.622 kb on - strand at 2041.734 kb on - strand at 2041.734 kb on - strand at 2041.734 kb on - strand at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.042 kb on + strand, within Pf1N1B4_1788 at 2042.042 kb on + strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.196 kb on - strand, within Pf1N1B4_1788 at 2042.383 kb on - strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.641 kb on + strand, within Pf1N1B4_1789
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,039,546 + Pf1N1B4_1786 0.62 +2.6 2,039,552 + Pf1N1B4_1786 0.63 +2.8 2,039,552 + Pf1N1B4_1786 0.63 +0.9 2,039,553 - Pf1N1B4_1786 0.63 -1.1 2,039,553 - Pf1N1B4_1786 0.63 -0.8 2,039,553 - Pf1N1B4_1786 0.63 -0.4 2,039,576 + Pf1N1B4_1786 0.64 -0.1 2,039,576 + Pf1N1B4_1786 0.64 -0.5 2,039,576 + Pf1N1B4_1786 0.64 +0.5 2,039,576 + Pf1N1B4_1786 0.64 +0.4 2,039,576 + Pf1N1B4_1786 0.64 +1.2 2,039,577 - +0.2 2,039,577 - Pf1N1B4_1786 0.64 +0.4 2,039,577 - Pf1N1B4_1786 0.64 +0.1 2,039,577 - Pf1N1B4_1786 0.64 +2.1 2,039,699 + Pf1N1B4_1786 0.70 -1.3 2,039,699 + Pf1N1B4_1786 0.70 +0.2 2,039,700 - Pf1N1B4_1786 0.70 +0.2 2,039,708 + Pf1N1B4_1786 0.71 +1.1 2,039,709 - Pf1N1B4_1786 0.71 -1.1 2,039,747 + Pf1N1B4_1786 0.73 -0.8 2,039,748 - Pf1N1B4_1786 0.73 -1.2 2,039,748 - Pf1N1B4_1786 0.73 +0.8 2,039,802 - Pf1N1B4_1786 0.76 -0.8 2,039,802 - Pf1N1B4_1786 0.76 +0.2 2,039,802 - Pf1N1B4_1786 0.76 -2.1 2,039,858 + Pf1N1B4_1786 0.78 -0.3 2,039,859 - Pf1N1B4_1786 0.79 +0.8 2,039,954 + Pf1N1B4_1786 0.83 +0.2 2,039,955 - Pf1N1B4_1786 0.83 +0.2 2,039,955 - Pf1N1B4_1786 0.83 +0.4 2,039,955 - Pf1N1B4_1786 0.83 +1.7 2,040,075 + Pf1N1B4_1786 0.90 -1.1 2,040,075 + +1.2 2,040,075 + Pf1N1B4_1786 0.90 -2.1 2,040,075 + Pf1N1B4_1786 0.90 +0.8 2,040,075 + Pf1N1B4_1786 0.90 -0.6 2,040,075 + Pf1N1B4_1786 0.90 +0.2 2,040,076 - Pf1N1B4_1786 0.90 +1.2 2,040,076 - Pf1N1B4_1786 0.90 +1.0 2,040,076 - Pf1N1B4_1786 0.90 +0.5 2,040,175 + -1.3 2,040,176 - -0.3 2,040,225 - +1.8 2,040,401 - +1.2 2,040,401 - -0.2 2,040,462 - +1.2 2,040,462 - -1.3 2,040,488 - +2.8 2,040,665 + Pf1N1B4_1787 0.13 +0.5 2,040,665 + Pf1N1B4_1787 0.13 +0.2 2,040,666 - Pf1N1B4_1787 0.13 -1.1 2,040,666 - Pf1N1B4_1787 0.13 +0.5 2,040,666 - Pf1N1B4_1787 0.13 +0.5 2,040,666 - Pf1N1B4_1787 0.13 +0.4 2,040,868 + Pf1N1B4_1787 0.30 -0.8 2,040,868 + Pf1N1B4_1787 0.30 +0.4 2,040,868 + Pf1N1B4_1787 0.30 -1.3 2,040,868 + Pf1N1B4_1787 0.30 +1.2 2,040,868 + Pf1N1B4_1787 0.30 -0.3 2,040,869 - Pf1N1B4_1787 0.30 +0.2 2,040,869 - Pf1N1B4_1787 0.30 -0.3 2,041,021 - Pf1N1B4_1787 0.42 +0.2 2,041,103 + Pf1N1B4_1787 0.49 +0.2 2,041,103 + Pf1N1B4_1787 0.49 +0.1 2,041,103 + Pf1N1B4_1787 0.49 -0.1 2,041,104 - Pf1N1B4_1787 0.49 +0.7 2,041,155 + Pf1N1B4_1787 0.53 +1.2 2,041,156 - Pf1N1B4_1787 0.53 -0.8 2,041,156 - Pf1N1B4_1787 0.53 +1.4 2,041,156 - Pf1N1B4_1787 0.53 -1.8 2,041,156 - Pf1N1B4_1787 0.53 +0.2 2,041,205 + Pf1N1B4_1787 0.57 +1.8 2,041,205 + Pf1N1B4_1787 0.57 +0.2 2,041,205 + Pf1N1B4_1787 0.57 +1.7 2,041,205 + Pf1N1B4_1787 0.57 -0.7 2,041,205 + Pf1N1B4_1787 0.57 +1.2 2,041,205 + Pf1N1B4_1787 0.57 -2.2 2,041,206 - Pf1N1B4_1787 0.57 +0.7 2,041,206 - Pf1N1B4_1787 0.57 +0.9 2,041,337 + Pf1N1B4_1787 0.68 +1.0 2,041,338 - Pf1N1B4_1787 0.68 +0.5 2,041,340 + Pf1N1B4_1787 0.68 +0.1 2,041,340 + Pf1N1B4_1787 0.68 -0.2 2,041,340 + Pf1N1B4_1787 0.68 +1.2 2,041,341 - Pf1N1B4_1787 0.68 +0.2 2,041,547 + Pf1N1B4_1787 0.85 +2.2 2,041,547 + Pf1N1B4_1787 0.85 +0.8 2,041,548 - Pf1N1B4_1787 0.85 +0.2 2,041,622 - -1.3 2,041,734 - -0.3 2,041,734 - -1.2 2,041,734 - +0.5 2,041,985 + Pf1N1B4_1788 0.18 -0.3 2,041,985 + Pf1N1B4_1788 0.18 -0.1 2,041,985 + Pf1N1B4_1788 0.18 -0.1 2,041,985 + Pf1N1B4_1788 0.18 +1.6 2,041,985 + Pf1N1B4_1788 0.18 +1.7 2,041,985 + Pf1N1B4_1788 0.18 +0.7 2,041,985 + Pf1N1B4_1788 0.18 -0.8 2,041,985 + Pf1N1B4_1788 0.18 -1.8 2,041,986 - Pf1N1B4_1788 0.18 +0.7 2,041,986 - Pf1N1B4_1788 0.18 -0.8 2,041,986 - +0.2 2,041,986 - Pf1N1B4_1788 0.18 -1.2 2,041,986 - Pf1N1B4_1788 0.18 -0.3 2,042,009 + Pf1N1B4_1788 0.24 +0.6 2,042,009 + Pf1N1B4_1788 0.24 +0.8 2,042,009 + Pf1N1B4_1788 0.24 -0.3 2,042,009 + Pf1N1B4_1788 0.24 +2.1 2,042,010 - Pf1N1B4_1788 0.24 -0.3 2,042,010 - Pf1N1B4_1788 0.24 +2.8 2,042,010 - Pf1N1B4_1788 0.24 -1.1 2,042,010 - Pf1N1B4_1788 0.24 -1.3 2,042,010 - Pf1N1B4_1788 0.24 -0.6 2,042,010 - Pf1N1B4_1788 0.24 -0.8 2,042,012 + Pf1N1B4_1788 0.24 +1.5 2,042,012 + Pf1N1B4_1788 0.24 -2.8 2,042,012 + Pf1N1B4_1788 0.24 -0.1 2,042,012 + Pf1N1B4_1788 0.24 +0.7 2,042,013 - Pf1N1B4_1788 0.25 -0.7 2,042,013 - Pf1N1B4_1788 0.25 +0.8 2,042,013 - Pf1N1B4_1788 0.25 -0.8 2,042,042 + Pf1N1B4_1788 0.31 +0.4 2,042,042 + Pf1N1B4_1788 0.31 +0.1 2,042,043 - Pf1N1B4_1788 0.31 +0.6 2,042,043 - Pf1N1B4_1788 0.31 -0.9 2,042,043 - Pf1N1B4_1788 0.31 -1.1 2,042,043 - Pf1N1B4_1788 0.31 +0.5 2,042,117 + Pf1N1B4_1788 0.49 -1.1 2,042,117 + Pf1N1B4_1788 0.49 +0.5 2,042,117 + Pf1N1B4_1788 0.49 -0.5 2,042,118 - Pf1N1B4_1788 0.49 -0.2 2,042,118 - Pf1N1B4_1788 0.49 -1.2 2,042,118 - Pf1N1B4_1788 0.49 -0.1 2,042,118 - Pf1N1B4_1788 0.49 -1.8 2,042,196 - Pf1N1B4_1788 0.67 +0.9 2,042,383 - -1.9 2,042,428 + +0.9 2,042,428 + +0.3 2,042,428 + +2.1 2,042,428 + +0.2 2,042,429 - +1.0 2,042,429 - -0.5 2,042,429 - -2.3 2,042,429 - -0.9 2,042,429 - -0.0 2,042,429 - -0.3 2,042,503 - Pf1N1B4_1789 0.19 +0.7 2,042,503 - Pf1N1B4_1789 0.19 +0.3 2,042,503 - Pf1N1B4_1789 0.19 +0.7 2,042,553 + Pf1N1B4_1789 0.30 -1.3 2,042,553 + Pf1N1B4_1789 0.30 +1.0 2,042,553 + Pf1N1B4_1789 0.30 +0.2 2,042,553 + Pf1N1B4_1789 0.30 +0.7 2,042,553 + Pf1N1B4_1789 0.30 -0.9 2,042,553 + Pf1N1B4_1789 0.30 +0.1 2,042,553 + Pf1N1B4_1789 0.30 -1.1 2,042,554 - Pf1N1B4_1789 0.30 +0.5 2,042,554 - Pf1N1B4_1789 0.30 +1.5 2,042,554 - Pf1N1B4_1789 0.30 -1.2 2,042,554 - Pf1N1B4_1789 0.30 -1.0 2,042,554 - Pf1N1B4_1789 0.30 -2.8 2,042,554 - Pf1N1B4_1789 0.30 +0.8 2,042,554 - Pf1N1B4_1789 0.30 -1.1 2,042,554 - Pf1N1B4_1789 0.30 +0.2 2,042,554 - Pf1N1B4_1789 0.30 -0.5 2,042,554 - Pf1N1B4_1789 0.30 +0.4 2,042,554 - Pf1N1B4_1789 0.30 +0.2 2,042,554 - Pf1N1B4_1789 0.30 -0.7 2,042,554 - Pf1N1B4_1789 0.30 -0.9 2,042,554 - Pf1N1B4_1789 0.30 -1.3 2,042,641 + Pf1N1B4_1789 0.48 +1.2
Or see this region's nucleotide sequence