Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1385 and Pf1N1B4_1386 are separated by 230 nucleotides Pf1N1B4_1386 and Pf1N1B4_1387 are separated by 4 nucleotides
Pf1N1B4_1385 - Transcriptional regulator, LysR family, at 1,585,421 to 1,586,332
_1385
Pf1N1B4_1386 - Nicotinate phosphoribosyltransferase (EC 2.4.2.11), at 1,586,563 to 1,587,789
_1386
Pf1N1B4_1387 - NAD synthetase (EC 6.3.1.5), at 1,587,794 to 1,588,621
_1387
Position (kb)
1586
1587
1588 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1585.631 kb on + strand, within Pf1N1B4_1385 at 1585.631 kb on + strand, within Pf1N1B4_1385 at 1585.632 kb on - strand, within Pf1N1B4_1385 at 1585.632 kb on - strand, within Pf1N1B4_1385 at 1585.632 kb on - strand, within Pf1N1B4_1385 at 1585.632 kb on - strand, within Pf1N1B4_1385 at 1585.632 kb on - strand, within Pf1N1B4_1385 at 1585.682 kb on + strand, within Pf1N1B4_1385 at 1585.682 kb on + strand, within Pf1N1B4_1385 at 1585.683 kb on - strand, within Pf1N1B4_1385 at 1585.683 kb on - strand, within Pf1N1B4_1385 at 1585.687 kb on - strand, within Pf1N1B4_1385 at 1585.687 kb on - strand, within Pf1N1B4_1385 at 1585.687 kb on - strand, within Pf1N1B4_1385 at 1585.865 kb on + strand, within Pf1N1B4_1385 at 1585.865 kb on + strand, within Pf1N1B4_1385 at 1585.866 kb on - strand at 1585.866 kb on - strand, within Pf1N1B4_1385 at 1585.866 kb on - strand, within Pf1N1B4_1385 at 1585.976 kb on - strand, within Pf1N1B4_1385 at 1586.055 kb on - strand, within Pf1N1B4_1385 at 1586.072 kb on + strand, within Pf1N1B4_1385 at 1586.072 kb on + strand, within Pf1N1B4_1385 at 1586.072 kb on + strand, within Pf1N1B4_1385 at 1586.073 kb on - strand, within Pf1N1B4_1385 at 1586.099 kb on + strand, within Pf1N1B4_1385 at 1586.099 kb on + strand, within Pf1N1B4_1385 at 1586.099 kb on + strand, within Pf1N1B4_1385 at 1586.099 kb on + strand, within Pf1N1B4_1385 at 1586.099 kb on + strand, within Pf1N1B4_1385 at 1586.099 kb on + strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.100 kb on - strand, within Pf1N1B4_1385 at 1586.228 kb on + strand, within Pf1N1B4_1385 at 1586.228 kb on + strand, within Pf1N1B4_1385 at 1586.228 kb on + strand, within Pf1N1B4_1385 at 1586.229 kb on - strand, within Pf1N1B4_1385 at 1586.231 kb on + strand, within Pf1N1B4_1385 at 1586.231 kb on + strand, within Pf1N1B4_1385 at 1586.232 kb on - strand, within Pf1N1B4_1385 at 1586.262 kb on + strand at 1586.262 kb on + strand at 1586.262 kb on + strand at 1586.263 kb on - strand at 1586.263 kb on - strand at 1586.263 kb on - strand at 1586.319 kb on + strand at 1586.320 kb on - strand at 1586.331 kb on - strand at 1586.331 kb on - strand at 1586.346 kb on - strand at 1586.409 kb on + strand at 1586.410 kb on - strand at 1586.456 kb on - strand at 1586.495 kb on + strand at 1586.620 kb on + strand at 1586.620 kb on + strand at 1586.620 kb on + strand at 1586.621 kb on - strand at 1586.621 kb on - strand at 1586.621 kb on - strand at 1586.717 kb on - strand, within Pf1N1B4_1386 at 1586.717 kb on - strand, within Pf1N1B4_1386 at 1586.717 kb on - strand, within Pf1N1B4_1386 at 1586.717 kb on - strand, within Pf1N1B4_1386 at 1586.857 kb on + strand, within Pf1N1B4_1386 at 1586.858 kb on - strand, within Pf1N1B4_1386 at 1586.858 kb on - strand, within Pf1N1B4_1386 at 1586.858 kb on - strand, within Pf1N1B4_1386 at 1586.860 kb on - strand, within Pf1N1B4_1386 at 1586.860 kb on - strand, within Pf1N1B4_1386 at 1587.120 kb on - strand, within Pf1N1B4_1386 at 1587.643 kb on - strand, within Pf1N1B4_1386 at 1587.708 kb on + strand at 1587.708 kb on + strand at 1587.708 kb on + strand at 1587.708 kb on + strand at 1587.708 kb on + strand at 1587.708 kb on + strand at 1587.752 kb on + strand at 1587.752 kb on + strand at 1587.752 kb on + strand at 1587.752 kb on + strand at 1587.752 kb on + strand at 1587.753 kb on - strand at 1587.771 kb on + strand at 1587.771 kb on + strand at 1587.771 kb on + strand at 1587.771 kb on + strand at 1588.755 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,585,631 + Pf1N1B4_1385 0.23 +0.4 1,585,631 + Pf1N1B4_1385 0.23 -0.5 1,585,632 - Pf1N1B4_1385 0.23 +0.4 1,585,632 - Pf1N1B4_1385 0.23 +0.8 1,585,632 - Pf1N1B4_1385 0.23 -0.6 1,585,632 - Pf1N1B4_1385 0.23 +1.7 1,585,632 - Pf1N1B4_1385 0.23 +0.1 1,585,682 + Pf1N1B4_1385 0.29 -0.4 1,585,682 + Pf1N1B4_1385 0.29 -1.2 1,585,683 - Pf1N1B4_1385 0.29 +0.1 1,585,683 - Pf1N1B4_1385 0.29 -0.3 1,585,687 - Pf1N1B4_1385 0.29 -0.1 1,585,687 - Pf1N1B4_1385 0.29 +1.4 1,585,687 - Pf1N1B4_1385 0.29 -1.2 1,585,865 + Pf1N1B4_1385 0.49 +0.8 1,585,865 + Pf1N1B4_1385 0.49 +0.7 1,585,866 - +1.1 1,585,866 - Pf1N1B4_1385 0.49 -0.5 1,585,866 - Pf1N1B4_1385 0.49 +0.5 1,585,976 - Pf1N1B4_1385 0.61 -0.9 1,586,055 - Pf1N1B4_1385 0.70 +0.9 1,586,072 + Pf1N1B4_1385 0.71 -0.6 1,586,072 + Pf1N1B4_1385 0.71 -0.5 1,586,072 + Pf1N1B4_1385 0.71 +1.4 1,586,073 - Pf1N1B4_1385 0.71 -1.7 1,586,099 + Pf1N1B4_1385 0.74 +0.2 1,586,099 + Pf1N1B4_1385 0.74 +1.3 1,586,099 + Pf1N1B4_1385 0.74 -0.5 1,586,099 + Pf1N1B4_1385 0.74 -0.5 1,586,099 + Pf1N1B4_1385 0.74 -1.3 1,586,099 + Pf1N1B4_1385 0.74 +1.1 1,586,100 - Pf1N1B4_1385 0.74 -0.7 1,586,100 - Pf1N1B4_1385 0.74 -0.5 1,586,100 - Pf1N1B4_1385 0.74 +0.4 1,586,100 - Pf1N1B4_1385 0.74 +0.4 1,586,100 - Pf1N1B4_1385 0.74 -1.7 1,586,100 - Pf1N1B4_1385 0.74 -1.7 1,586,100 - Pf1N1B4_1385 0.74 -0.9 1,586,100 - Pf1N1B4_1385 0.74 +0.5 1,586,100 - Pf1N1B4_1385 0.74 -0.2 1,586,100 - Pf1N1B4_1385 0.74 +0.5 1,586,100 - Pf1N1B4_1385 0.74 -1.1 1,586,100 - Pf1N1B4_1385 0.74 -2.9 1,586,100 - Pf1N1B4_1385 0.74 +0.9 1,586,100 - Pf1N1B4_1385 0.74 +0.3 1,586,228 + Pf1N1B4_1385 0.88 -1.3 1,586,228 + Pf1N1B4_1385 0.88 +1.1 1,586,228 + Pf1N1B4_1385 0.88 +0.5 1,586,229 - Pf1N1B4_1385 0.89 -2.6 1,586,231 + Pf1N1B4_1385 0.89 +0.1 1,586,231 + Pf1N1B4_1385 0.89 -0.0 1,586,232 - Pf1N1B4_1385 0.89 +0.6 1,586,262 + +3.1 1,586,262 + -0.9 1,586,262 + -0.1 1,586,263 - +1.3 1,586,263 - -0.5 1,586,263 - -0.9 1,586,319 + -0.2 1,586,320 - -1.2 1,586,331 - +0.6 1,586,331 - +0.8 1,586,346 - +0.4 1,586,409 + -0.0 1,586,410 - +0.2 1,586,456 - -1.9 1,586,495 + +0.9 1,586,620 + -0.5 1,586,620 + -0.5 1,586,620 + +0.6 1,586,621 - -2.6 1,586,621 - -0.5 1,586,621 - -0.5 1,586,717 - Pf1N1B4_1386 0.13 +0.1 1,586,717 - Pf1N1B4_1386 0.13 +0.1 1,586,717 - Pf1N1B4_1386 0.13 -0.9 1,586,717 - Pf1N1B4_1386 0.13 -0.3 1,586,857 + Pf1N1B4_1386 0.24 -0.3 1,586,858 - Pf1N1B4_1386 0.24 -1.5 1,586,858 - Pf1N1B4_1386 0.24 -2.6 1,586,858 - Pf1N1B4_1386 0.24 -0.6 1,586,860 - Pf1N1B4_1386 0.24 -1.5 1,586,860 - Pf1N1B4_1386 0.24 -0.2 1,587,120 - Pf1N1B4_1386 0.45 -0.5 1,587,643 - Pf1N1B4_1386 0.88 -0.5 1,587,708 + -0.8 1,587,708 + -1.0 1,587,708 + -0.9 1,587,708 + -0.5 1,587,708 + +1.1 1,587,708 + -0.2 1,587,752 + +1.7 1,587,752 + +1.3 1,587,752 + +1.4 1,587,752 + -0.5 1,587,752 + +0.1 1,587,753 - -0.9 1,587,771 + +0.2 1,587,771 + -0.5 1,587,771 + -0.5 1,587,771 + +0.4 1,588,755 - -0.8
Or see this region's nucleotide sequence