Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1316 and Pf1N1B4_1317 are separated by 99 nucleotides Pf1N1B4_1317 and Pf1N1B4_1318 are separated by 49 nucleotides Pf1N1B4_1318 and Pf1N1B4_1319 are separated by 86 nucleotides
Pf1N1B4_1316 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 1,499,196 to 1,500,491
_1316
Pf1N1B4_1317 - Sensor histidine kinase/response regulator, at 1,500,591 to 1,503,365
_1317
Pf1N1B4_1318 - hypothetical protein, at 1,503,415 to 1,504,077
_1318
Pf1N1B4_1319 - hypothetical protein, at 1,504,164 to 1,504,949
_1319
Position (kb)
1500
1501
1502
1503
1504 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1499.592 kb on + strand, within Pf1N1B4_1316 at 1499.593 kb on - strand, within Pf1N1B4_1316 at 1499.616 kb on - strand, within Pf1N1B4_1316 at 1499.809 kb on + strand, within Pf1N1B4_1316 at 1500.489 kb on + strand at 1500.489 kb on + strand at 1500.490 kb on - strand at 1500.510 kb on + strand at 1500.536 kb on - strand at 1500.552 kb on + strand at 1500.552 kb on + strand at 1500.552 kb on + strand at 1500.552 kb on + strand at 1500.553 kb on - strand at 1500.553 kb on - strand at 1500.553 kb on - strand at 1500.556 kb on + strand at 1500.556 kb on + strand at 1500.556 kb on + strand at 1500.557 kb on - strand at 1500.557 kb on - strand at 1500.558 kb on + strand at 1500.558 kb on + strand at 1500.558 kb on + strand at 1500.559 kb on - strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.575 kb on - strand at 1500.575 kb on - strand at 1500.575 kb on - strand at 1500.888 kb on + strand, within Pf1N1B4_1317 at 1501.053 kb on + strand, within Pf1N1B4_1317 at 1501.466 kb on + strand, within Pf1N1B4_1317 at 1501.471 kb on - strand, within Pf1N1B4_1317 at 1501.894 kb on - strand, within Pf1N1B4_1317 at 1502.894 kb on + strand, within Pf1N1B4_1317 at 1503.007 kb on + strand, within Pf1N1B4_1317 at 1503.413 kb on + strand at 1503.414 kb on - strand at 1503.414 kb on - strand at 1503.414 kb on - strand at 1503.414 kb on - strand at 1503.562 kb on + strand, within Pf1N1B4_1318 at 1503.563 kb on - strand at 1503.563 kb on - strand, within Pf1N1B4_1318 at 1503.633 kb on - strand, within Pf1N1B4_1318 at 1503.761 kb on + strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.783 kb on - strand, within Pf1N1B4_1318 at 1503.823 kb on - strand, within Pf1N1B4_1318 at 1503.823 kb on - strand, within Pf1N1B4_1318 at 1503.938 kb on + strand, within Pf1N1B4_1318 at 1503.938 kb on + strand, within Pf1N1B4_1318 at 1503.939 kb on - strand, within Pf1N1B4_1318 at 1503.939 kb on - strand, within Pf1N1B4_1318 at 1504.015 kb on + strand at 1504.016 kb on - strand at 1504.016 kb on - strand at 1504.093 kb on + strand at 1504.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,499,592 + Pf1N1B4_1316 0.31 -1.5 1,499,593 - Pf1N1B4_1316 0.31 -1.2 1,499,616 - Pf1N1B4_1316 0.32 -1.2 1,499,809 + Pf1N1B4_1316 0.47 -2.8 1,500,489 + +1.9 1,500,489 + -1.9 1,500,490 - -0.2 1,500,510 + -1.5 1,500,536 - +0.1 1,500,552 + +1.1 1,500,552 + -0.1 1,500,552 + +0.5 1,500,552 + +1.0 1,500,553 - -0.1 1,500,553 - -0.9 1,500,553 - -2.2 1,500,556 + -0.9 1,500,556 + +0.5 1,500,556 + -0.7 1,500,557 - -1.2 1,500,557 - -0.5 1,500,558 + +0.1 1,500,558 + +0.5 1,500,558 + +2.1 1,500,559 - +0.1 1,500,574 + +0.1 1,500,574 + +0.5 1,500,574 + +1.1 1,500,574 + -0.3 1,500,574 + +0.1 1,500,574 + +0.7 1,500,575 - -0.8 1,500,575 - -1.2 1,500,575 - +1.7 1,500,888 + Pf1N1B4_1317 0.11 -0.9 1,501,053 + Pf1N1B4_1317 0.17 +0.4 1,501,466 + Pf1N1B4_1317 0.32 -0.9 1,501,471 - Pf1N1B4_1317 0.32 +0.1 1,501,894 - Pf1N1B4_1317 0.47 +0.1 1,502,894 + Pf1N1B4_1317 0.83 -0.3 1,503,007 + Pf1N1B4_1317 0.87 -1.2 1,503,413 + -2.2 1,503,414 - -0.3 1,503,414 - -0.9 1,503,414 - +0.6 1,503,414 - -2.1 1,503,562 + Pf1N1B4_1318 0.22 -0.5 1,503,563 - +0.5 1,503,563 - Pf1N1B4_1318 0.22 -1.2 1,503,633 - Pf1N1B4_1318 0.33 +2.2 1,503,761 + Pf1N1B4_1318 0.52 -0.2 1,503,762 - Pf1N1B4_1318 0.52 +0.3 1,503,762 - Pf1N1B4_1318 0.52 +0.3 1,503,762 - Pf1N1B4_1318 0.52 -2.2 1,503,762 - Pf1N1B4_1318 0.52 +0.5 1,503,762 - Pf1N1B4_1318 0.52 -0.6 1,503,783 - Pf1N1B4_1318 0.56 -0.5 1,503,823 - Pf1N1B4_1318 0.62 -1.5 1,503,823 - Pf1N1B4_1318 0.62 +0.4 1,503,938 + Pf1N1B4_1318 0.79 -0.7 1,503,938 + Pf1N1B4_1318 0.79 +0.3 1,503,939 - Pf1N1B4_1318 0.79 -0.5 1,503,939 - Pf1N1B4_1318 0.79 -1.2 1,504,015 + +1.7 1,504,016 - +0.1 1,504,016 - -0.8 1,504,093 + +0.1 1,504,093 + +0.8
Or see this region's nucleotide sequence