Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1309 and Pf1N1B4_1310 are separated by 326 nucleotides Pf1N1B4_1310 and Pf1N1B4_1311 are separated by 30 nucleotides
Pf1N1B4_1309 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 1,492,027 to 1,493,385
_1309
Pf1N1B4_1310 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 1,493,712 to 1,494,590
_1310
Pf1N1B4_1311 - Probable transmembrane protein, at 1,494,621 to 1,495,841
_1311
Position (kb)
1493
1494
1495 Strain fitness (log2 ratio)
-2
-1
0
1 at 1493.651 kb on - strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.690 kb on - strand at 1493.691 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.764 kb on - strand at 1494.088 kb on + strand, within Pf1N1B4_1310 at 1494.089 kb on - strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.348 kb on + strand, within Pf1N1B4_1310 at 1494.349 kb on - strand, within Pf1N1B4_1310 at 1494.385 kb on + strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.516 kb on + strand at 1494.573 kb on - strand at 1494.606 kb on - strand at 1494.681 kb on - strand at 1494.734 kb on - strand at 1494.754 kb on + strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1495.303 kb on + strand, within Pf1N1B4_1311 at 1495.304 kb on - strand, within Pf1N1B4_1311 at 1495.426 kb on - strand, within Pf1N1B4_1311 at 1495.435 kb on - strand, within Pf1N1B4_1311
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,493,651 - +0.1 1,493,689 + +0.1 1,493,689 + -0.6 1,493,689 + -1.5 1,493,689 + -0.2 1,493,690 - -0.2 1,493,691 + -0.0 1,493,763 + -2.6 1,493,763 + +0.1 1,493,763 + -0.5 1,493,764 - -0.3 1,494,088 + Pf1N1B4_1310 0.43 -1.5 1,494,089 - Pf1N1B4_1310 0.43 -0.0 1,494,323 + Pf1N1B4_1310 0.70 -1.2 1,494,323 + Pf1N1B4_1310 0.70 -2.1 1,494,323 + Pf1N1B4_1310 0.70 -0.5 1,494,323 + Pf1N1B4_1310 0.70 -1.1 1,494,324 - Pf1N1B4_1310 0.70 -0.9 1,494,324 - Pf1N1B4_1310 0.70 -1.1 1,494,324 - Pf1N1B4_1310 0.70 -1.6 1,494,324 - Pf1N1B4_1310 0.70 +0.3 1,494,348 + Pf1N1B4_1310 0.72 -0.3 1,494,349 - Pf1N1B4_1310 0.72 +0.9 1,494,385 + Pf1N1B4_1310 0.77 +0.2 1,494,386 - Pf1N1B4_1310 0.77 -1.2 1,494,386 - Pf1N1B4_1310 0.77 -1.2 1,494,386 - Pf1N1B4_1310 0.77 -0.7 1,494,516 + -0.9 1,494,573 - +0.5 1,494,606 - +1.1 1,494,681 - -0.7 1,494,734 - -0.3 1,494,754 + Pf1N1B4_1311 0.11 +0.7 1,494,755 - Pf1N1B4_1311 0.11 +0.3 1,494,755 - Pf1N1B4_1311 0.11 -0.1 1,495,303 + Pf1N1B4_1311 0.56 -2.2 1,495,304 - Pf1N1B4_1311 0.56 +0.3 1,495,426 - Pf1N1B4_1311 0.66 -0.2 1,495,435 - Pf1N1B4_1311 0.67 -0.2
Or see this region's nucleotide sequence