Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1292 and Pf1N1B4_1293 are separated by 19 nucleotides Pf1N1B4_1293 and Pf1N1B4_1294 overlap by 4 nucleotides Pf1N1B4_1294 and Pf1N1B4_1295 are separated by 24 nucleotides Pf1N1B4_1295 and Pf1N1B4_1296 are separated by 257 nucleotides
Pf1N1B4_1292 - DNA-damage-inducible protein F, at 1,472,913 to 1,474,253
_1292
Pf1N1B4_1293 - hypothetical protein, at 1,474,273 to 1,475,070
_1293
Pf1N1B4_1294 - VgrG protein, at 1,475,067 to 1,476,122
_1294
Pf1N1B4_1295 - hypothetical protein, at 1,476,147 to 1,476,368
_1295
Pf1N1B4_1296 - Biosynthetic arginine decarboxylase (EC 4.1.1.19) (from data) , at 1,476,626 to 1,478,539
_1296
Position (kb)
1475
1476
1477 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1474.088 kb on + strand at 1474.089 kb on - strand, within Pf1N1B4_1292 at 1474.218 kb on + strand at 1474.218 kb on + strand at 1474.219 kb on - strand at 1474.219 kb on - strand at 1474.442 kb on + strand, within Pf1N1B4_1293 at 1474.442 kb on + strand, within Pf1N1B4_1293 at 1474.443 kb on - strand, within Pf1N1B4_1293 at 1474.443 kb on - strand, within Pf1N1B4_1293 at 1474.460 kb on + strand at 1474.461 kb on - strand, within Pf1N1B4_1293 at 1474.712 kb on + strand, within Pf1N1B4_1293 at 1474.712 kb on + strand at 1474.733 kb on + strand, within Pf1N1B4_1293 at 1474.733 kb on + strand, within Pf1N1B4_1293 at 1474.733 kb on + strand, within Pf1N1B4_1293 at 1474.734 kb on - strand, within Pf1N1B4_1293 at 1474.736 kb on + strand, within Pf1N1B4_1293 at 1475.601 kb on - strand, within Pf1N1B4_1294 at 1475.602 kb on + strand, within Pf1N1B4_1294 at 1475.603 kb on - strand, within Pf1N1B4_1294 at 1475.890 kb on + strand, within Pf1N1B4_1294 at 1476.030 kb on + strand at 1476.031 kb on - strand at 1476.031 kb on - strand at 1476.129 kb on + strand at 1476.130 kb on - strand at 1476.226 kb on + strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.351 kb on + strand at 1476.351 kb on + strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.449 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.469 kb on + strand at 1476.470 kb on - strand at 1476.470 kb on - strand at 1476.481 kb on + strand at 1476.481 kb on + strand at 1476.482 kb on - strand at 1476.583 kb on + strand at 1476.584 kb on - strand at 1476.584 kb on - strand at 1476.622 kb on + strand at 1476.622 kb on + strand at 1476.623 kb on - strand at 1476.623 kb on - strand at 1476.639 kb on + strand at 1476.639 kb on + strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.765 kb on + strand at 1476.813 kb on + strand at 1476.814 kb on - strand at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.927 kb on + strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.954 kb on - strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,474,088 + +0.8 1,474,089 - Pf1N1B4_1292 0.88 +0.1 1,474,218 + +0.1 1,474,218 + +0.1 1,474,219 - +0.8 1,474,219 - -2.4 1,474,442 + Pf1N1B4_1293 0.21 -0.1 1,474,442 + Pf1N1B4_1293 0.21 -0.7 1,474,443 - Pf1N1B4_1293 0.21 +0.8 1,474,443 - Pf1N1B4_1293 0.21 +1.1 1,474,460 + +2.1 1,474,461 - Pf1N1B4_1293 0.24 +1.4 1,474,712 + Pf1N1B4_1293 0.55 +1.1 1,474,712 + +1.1 1,474,733 + Pf1N1B4_1293 0.58 -0.8 1,474,733 + Pf1N1B4_1293 0.58 +1.1 1,474,733 + Pf1N1B4_1293 0.58 -1.1 1,474,734 - Pf1N1B4_1293 0.58 -1.1 1,474,736 + Pf1N1B4_1293 0.58 -1.5 1,475,601 - Pf1N1B4_1294 0.51 -0.8 1,475,602 + Pf1N1B4_1294 0.51 +1.4 1,475,603 - Pf1N1B4_1294 0.51 -0.9 1,475,890 + Pf1N1B4_1294 0.78 -0.5 1,476,030 + -0.2 1,476,031 - -1.2 1,476,031 - -0.5 1,476,129 + -2.2 1,476,130 - +0.8 1,476,226 + Pf1N1B4_1295 0.36 -0.2 1,476,227 - Pf1N1B4_1295 0.36 -0.0 1,476,227 - Pf1N1B4_1295 0.36 +0.3 1,476,351 + -1.9 1,476,351 + +0.7 1,476,352 - +0.6 1,476,352 - +0.1 1,476,352 - +1.1 1,476,352 - -0.2 1,476,449 + -0.4 1,476,462 + -1.6 1,476,462 + +0.2 1,476,462 + +0.8 1,476,462 + -1.2 1,476,462 + -0.5 1,476,469 + -1.7 1,476,470 - +1.2 1,476,470 - +0.8 1,476,481 + +0.5 1,476,481 + -1.2 1,476,482 - +0.6 1,476,583 + +0.5 1,476,584 - -0.9 1,476,584 - +0.3 1,476,622 + +0.9 1,476,622 + -0.4 1,476,623 - -1.7 1,476,623 - -0.7 1,476,639 + +0.3 1,476,639 + -0.7 1,476,640 - +0.2 1,476,640 - -0.3 1,476,640 - -1.9 1,476,640 - -0.4 1,476,640 - -1.4 1,476,765 + -0.3 1,476,813 + +0.7 1,476,814 - -0.9 1,476,837 + Pf1N1B4_1296 0.11 -0.3 1,476,837 + Pf1N1B4_1296 0.11 -0.9 1,476,837 + Pf1N1B4_1296 0.11 +0.3 1,476,838 - Pf1N1B4_1296 0.11 -0.2 1,476,838 - Pf1N1B4_1296 0.11 +0.8 1,476,927 + Pf1N1B4_1296 0.16 -0.1 1,476,928 - Pf1N1B4_1296 0.16 -1.2 1,476,928 - Pf1N1B4_1296 0.16 -0.9 1,476,954 - Pf1N1B4_1296 0.17 -1.4 1,476,990 + Pf1N1B4_1296 0.19 -1.7 1,476,990 + Pf1N1B4_1296 0.19 -0.8 1,476,991 - Pf1N1B4_1296 0.19 +1.1 1,476,991 - Pf1N1B4_1296 0.19 -1.1 1,476,991 - Pf1N1B4_1296 0.19 +0.3
Or see this region's nucleotide sequence