Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1243 and Pf1N1B4_1244 are separated by 11 nucleotides Pf1N1B4_1244 and Pf1N1B4_1245 overlap by 1 nucleotides
Pf1N1B4_1243 - ABC transporter ATP-binding protein, at 1,421,360 to 1,422,925
_1243
Pf1N1B4_1244 - Nucleoside ABC transporter, permease protein 1, at 1,422,937 to 1,424,043
_1244
Pf1N1B4_1245 - Nucleoside ABC transporter, permease protein 2, at 1,424,043 to 1,424,969
_1245
Position (kb)
1422
1423
1424
1425 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1421.963 kb on - strand, within Pf1N1B4_1243 at 1423.108 kb on + strand, within Pf1N1B4_1244 at 1423.108 kb on + strand, within Pf1N1B4_1244 at 1423.108 kb on + strand, within Pf1N1B4_1244 at 1423.108 kb on + strand, within Pf1N1B4_1244 at 1423.109 kb on - strand, within Pf1N1B4_1244 at 1423.109 kb on - strand at 1423.399 kb on + strand, within Pf1N1B4_1244 at 1423.399 kb on + strand, within Pf1N1B4_1244 at 1423.399 kb on + strand, within Pf1N1B4_1244 at 1423.399 kb on + strand, within Pf1N1B4_1244 at 1423.400 kb on - strand, within Pf1N1B4_1244 at 1423.400 kb on - strand, within Pf1N1B4_1244 at 1423.400 kb on - strand, within Pf1N1B4_1244 at 1423.400 kb on - strand, within Pf1N1B4_1244 at 1423.400 kb on - strand, within Pf1N1B4_1244 at 1423.400 kb on - strand, within Pf1N1B4_1244 at 1423.543 kb on + strand, within Pf1N1B4_1244 at 1423.544 kb on - strand, within Pf1N1B4_1244 at 1423.544 kb on - strand, within Pf1N1B4_1244 at 1423.861 kb on + strand at 1423.861 kb on + strand, within Pf1N1B4_1244 at 1423.861 kb on + strand, within Pf1N1B4_1244 at 1423.862 kb on - strand, within Pf1N1B4_1244 at 1423.862 kb on - strand, within Pf1N1B4_1244 at 1423.862 kb on - strand, within Pf1N1B4_1244 at 1423.961 kb on + strand at 1423.962 kb on - strand at 1423.962 kb on - strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.067 kb on - strand at 1424.073 kb on + strand at 1424.199 kb on - strand, within Pf1N1B4_1245 at 1424.199 kb on - strand, within Pf1N1B4_1245 at 1424.478 kb on + strand, within Pf1N1B4_1245 at 1424.478 kb on + strand, within Pf1N1B4_1245 at 1424.478 kb on + strand, within Pf1N1B4_1245 at 1424.479 kb on - strand, within Pf1N1B4_1245 at 1424.479 kb on - strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.659 kb on - strand, within Pf1N1B4_1245 at 1424.673 kb on + strand, within Pf1N1B4_1245 at 1424.673 kb on + strand, within Pf1N1B4_1245 at 1424.674 kb on - strand, within Pf1N1B4_1245 at 1424.674 kb on - strand, within Pf1N1B4_1245 at 1424.674 kb on - strand, within Pf1N1B4_1245 at 1424.778 kb on + strand, within Pf1N1B4_1245 at 1424.779 kb on - strand, within Pf1N1B4_1245 at 1424.865 kb on + strand, within Pf1N1B4_1245 at 1424.865 kb on + strand, within Pf1N1B4_1245 at 1424.865 kb on + strand at 1424.865 kb on + strand, within Pf1N1B4_1245 at 1424.866 kb on - strand, within Pf1N1B4_1245 at 1424.925 kb on + strand at 1424.925 kb on + strand at 1424.925 kb on + strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.968 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,421,963 - Pf1N1B4_1243 0.39 -2.7 1,423,108 + Pf1N1B4_1244 0.15 +1.4 1,423,108 + Pf1N1B4_1244 0.15 +0.4 1,423,108 + Pf1N1B4_1244 0.15 -0.2 1,423,108 + Pf1N1B4_1244 0.15 +0.1 1,423,109 - Pf1N1B4_1244 0.16 -0.3 1,423,109 - +0.5 1,423,399 + Pf1N1B4_1244 0.42 +1.1 1,423,399 + Pf1N1B4_1244 0.42 -0.1 1,423,399 + Pf1N1B4_1244 0.42 +2.3 1,423,399 + Pf1N1B4_1244 0.42 -0.9 1,423,400 - Pf1N1B4_1244 0.42 +1.1 1,423,400 - Pf1N1B4_1244 0.42 +0.1 1,423,400 - Pf1N1B4_1244 0.42 -1.7 1,423,400 - Pf1N1B4_1244 0.42 -0.2 1,423,400 - Pf1N1B4_1244 0.42 -1.7 1,423,400 - Pf1N1B4_1244 0.42 +1.9 1,423,543 + Pf1N1B4_1244 0.55 +0.8 1,423,544 - Pf1N1B4_1244 0.55 -0.2 1,423,544 - Pf1N1B4_1244 0.55 -0.3 1,423,861 + -0.9 1,423,861 + Pf1N1B4_1244 0.83 +2.1 1,423,861 + Pf1N1B4_1244 0.83 +0.5 1,423,862 - Pf1N1B4_1244 0.84 -0.9 1,423,862 - Pf1N1B4_1244 0.84 +1.1 1,423,862 - Pf1N1B4_1244 0.84 -0.3 1,423,961 + +2.2 1,423,962 - +0.3 1,423,962 - +1.4 1,424,066 + +1.1 1,424,066 + +0.1 1,424,066 + +0.3 1,424,066 + +1.5 1,424,066 + -0.7 1,424,066 + +0.7 1,424,067 - +1.3 1,424,073 + +0.3 1,424,199 - Pf1N1B4_1245 0.17 -0.1 1,424,199 - Pf1N1B4_1245 0.17 +2.1 1,424,478 + Pf1N1B4_1245 0.47 -0.1 1,424,478 + Pf1N1B4_1245 0.47 +1.1 1,424,478 + Pf1N1B4_1245 0.47 -1.3 1,424,479 - Pf1N1B4_1245 0.47 -1.1 1,424,479 - Pf1N1B4_1245 0.47 +0.1 1,424,658 + Pf1N1B4_1245 0.66 +0.5 1,424,658 + Pf1N1B4_1245 0.66 +1.1 1,424,658 + Pf1N1B4_1245 0.66 -0.1 1,424,658 + Pf1N1B4_1245 0.66 -0.9 1,424,659 - Pf1N1B4_1245 0.66 -0.4 1,424,673 + Pf1N1B4_1245 0.68 +0.1 1,424,673 + Pf1N1B4_1245 0.68 +1.1 1,424,674 - Pf1N1B4_1245 0.68 -0.5 1,424,674 - Pf1N1B4_1245 0.68 +0.4 1,424,674 - Pf1N1B4_1245 0.68 -0.1 1,424,778 + Pf1N1B4_1245 0.79 +1.2 1,424,779 - Pf1N1B4_1245 0.79 -0.3 1,424,865 + Pf1N1B4_1245 0.89 +0.6 1,424,865 + Pf1N1B4_1245 0.89 +1.1 1,424,865 + +1.6 1,424,865 + Pf1N1B4_1245 0.89 -0.2 1,424,866 - Pf1N1B4_1245 0.89 -0.5 1,424,925 + +0.3 1,424,925 + -0.8 1,424,925 + +0.1 1,424,926 - -0.7 1,424,926 - +0.8 1,424,926 - -2.1 1,424,926 - +1.1 1,424,926 - +0.3 1,424,968 - +0.6
Or see this region's nucleotide sequence