Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1127 and Pf1N1B4_1128 are separated by 169 nucleotides Pf1N1B4_1128 and Pf1N1B4_1129 are separated by 351 nucleotides Pf1N1B4_1129 and Pf1N1B4_1130 overlap by 19 nucleotides
Pf1N1B4_1127 - Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2), at 1,265,723 to 1,266,703
_1127
Pf1N1B4_1128 - hypothetical protein, at 1,266,873 to 1,267,088
_1128
Pf1N1B4_1129 - Peptide chain release factor 3, at 1,267,440 to 1,267,691
_1129
Pf1N1B4_1130 - Peptide chain release factor 3, at 1,267,673 to 1,269,022
_1130
Position (kb)
1266
1267
1268 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1266.319 kb on - strand, within Pf1N1B4_1127 at 1266.319 kb on - strand, within Pf1N1B4_1127 at 1266.319 kb on - strand at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.342 kb on - strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.405 kb on - strand, within Pf1N1B4_1127 at 1266.405 kb on - strand, within Pf1N1B4_1127 at 1266.446 kb on + strand at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.671 kb on + strand at 1266.671 kb on + strand at 1266.701 kb on + strand at 1266.874 kb on + strand at 1266.874 kb on + strand at 1266.875 kb on - strand at 1266.875 kb on - strand at 1266.875 kb on - strand at 1267.055 kb on + strand, within Pf1N1B4_1128 at 1267.055 kb on + strand, within Pf1N1B4_1128 at 1267.055 kb on + strand, within Pf1N1B4_1128 at 1267.056 kb on - strand, within Pf1N1B4_1128 at 1267.056 kb on - strand, within Pf1N1B4_1128 at 1267.078 kb on + strand at 1267.078 kb on + strand at 1267.078 kb on + strand at 1267.078 kb on + strand at 1267.079 kb on - strand at 1267.079 kb on - strand at 1267.079 kb on - strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.083 kb on - strand at 1267.083 kb on - strand at 1267.083 kb on - strand at 1267.083 kb on - strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.089 kb on - strand at 1267.134 kb on + strand at 1267.148 kb on + strand at 1267.148 kb on + strand at 1267.149 kb on - strand at 1267.235 kb on + strand at 1267.235 kb on + strand at 1267.236 kb on - strand at 1267.237 kb on + strand at 1267.407 kb on - strand at 1267.407 kb on - strand at 1267.407 kb on - strand at 1267.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,266,319 - Pf1N1B4_1127 0.61 -0.2 1,266,319 - Pf1N1B4_1127 0.61 +0.5 1,266,319 - +2.6 1,266,341 + Pf1N1B4_1127 0.63 -0.2 1,266,341 + Pf1N1B4_1127 0.63 -0.1 1,266,341 + Pf1N1B4_1127 0.63 -0.6 1,266,341 + Pf1N1B4_1127 0.63 +0.4 1,266,342 - Pf1N1B4_1127 0.63 +1.7 1,266,404 + Pf1N1B4_1127 0.69 +0.6 1,266,404 + Pf1N1B4_1127 0.69 +1.3 1,266,404 + Pf1N1B4_1127 0.69 -0.1 1,266,404 + Pf1N1B4_1127 0.69 +0.1 1,266,404 + Pf1N1B4_1127 0.69 +0.0 1,266,404 + Pf1N1B4_1127 0.69 -1.0 1,266,405 - Pf1N1B4_1127 0.70 +0.7 1,266,405 - Pf1N1B4_1127 0.70 +0.1 1,266,446 + +1.0 1,266,446 + Pf1N1B4_1127 0.74 +1.0 1,266,446 + Pf1N1B4_1127 0.74 -2.6 1,266,446 + Pf1N1B4_1127 0.74 +1.6 1,266,446 + Pf1N1B4_1127 0.74 +0.4 1,266,446 + Pf1N1B4_1127 0.74 -1.0 1,266,446 + Pf1N1B4_1127 0.74 -0.6 1,266,446 + +2.0 1,266,446 + Pf1N1B4_1127 0.74 +0.3 1,266,447 - Pf1N1B4_1127 0.74 +0.7 1,266,447 - Pf1N1B4_1127 0.74 -2.2 1,266,447 - Pf1N1B4_1127 0.74 -1.6 1,266,447 - Pf1N1B4_1127 0.74 +1.4 1,266,447 - Pf1N1B4_1127 0.74 +0.4 1,266,671 + -1.1 1,266,671 + +0.7 1,266,701 + +1.5 1,266,874 + -1.8 1,266,874 + +2.0 1,266,875 - -0.2 1,266,875 - +1.0 1,266,875 - -1.3 1,267,055 + Pf1N1B4_1128 0.84 -0.4 1,267,055 + Pf1N1B4_1128 0.84 -0.6 1,267,055 + Pf1N1B4_1128 0.84 -2.3 1,267,056 - Pf1N1B4_1128 0.85 -0.4 1,267,056 - Pf1N1B4_1128 0.85 -0.2 1,267,078 + -0.7 1,267,078 + +0.0 1,267,078 + -1.4 1,267,078 + +1.6 1,267,079 - +1.8 1,267,079 - -0.6 1,267,079 - +0.4 1,267,082 + -0.4 1,267,082 + +4.3 1,267,082 + -1.0 1,267,082 + -1.0 1,267,082 + +0.9 1,267,082 + -1.0 1,267,082 + -0.2 1,267,083 - -0.6 1,267,083 - +0.2 1,267,083 - +0.4 1,267,083 - -0.2 1,267,088 + -1.3 1,267,088 + +0.0 1,267,088 + -0.8 1,267,088 + -0.6 1,267,088 + +1.0 1,267,088 + +0.4 1,267,089 - +0.5 1,267,134 + +0.1 1,267,148 + +0.2 1,267,148 + -1.6 1,267,149 - -1.0 1,267,235 + -0.7 1,267,235 + -1.3 1,267,236 - +0.1 1,267,237 + +0.8 1,267,407 - +2.0 1,267,407 - +0.4 1,267,407 - -0.8 1,267,428 - +0.0
Or see this region's nucleotide sequence