Experiment: PYE with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02491 and CCNA_02492 overlap by 8 nucleotides CCNA_02492 and CCNA_02493 are separated by 19 nucleotides CCNA_02493 and CCNA_02494 overlap by 1 nucleotides
CCNA_02491: CCNA_02491 - protocatechuate 3,4-dioxygenase beta chain, at 2,638,974 to 2,639,681
_02491
CCNA_02492: CCNA_02492 - protocatechuate 3,4-dioxygenase alpha chain, at 2,639,674 to 2,640,324
_02492
CCNA_02493: CCNA_02493 - 3-carboxy-cis,cis-muconate cycloisomerase, at 2,640,344 to 2,641,492
_02493
CCNA_02494: CCNA_02494 - 3-oxoadipate enol-lactonase/4-carboxymuconolactone decarboxylase, at 2,641,492 to 2,642,673
_02494
Position (kb)
2640
2641
2642 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2639.362 kb on - strand, within CCNA_02491 at 2639.393 kb on + strand, within CCNA_02491 at 2639.465 kb on - strand, within CCNA_02491 at 2639.484 kb on + strand, within CCNA_02491 at 2639.492 kb on - strand, within CCNA_02491 at 2639.492 kb on - strand, within CCNA_02491 at 2639.562 kb on + strand, within CCNA_02491 at 2639.562 kb on + strand, within CCNA_02491 at 2639.597 kb on + strand, within CCNA_02491 at 2639.600 kb on + strand, within CCNA_02491 at 2639.605 kb on - strand, within CCNA_02491 at 2639.605 kb on - strand, within CCNA_02491 at 2639.608 kb on - strand, within CCNA_02491 at 2639.612 kb on + strand at 2639.612 kb on + strand at 2639.634 kb on - strand at 2639.639 kb on - strand at 2639.641 kb on + strand at 2639.645 kb on + strand at 2639.653 kb on - strand at 2639.668 kb on + strand at 2639.698 kb on - strand at 2639.725 kb on - strand at 2639.761 kb on + strand, within CCNA_02492 at 2639.776 kb on - strand, within CCNA_02492 at 2639.786 kb on - strand, within CCNA_02492 at 2639.786 kb on - strand, within CCNA_02492 at 2639.795 kb on - strand, within CCNA_02492 at 2639.800 kb on - strand, within CCNA_02492 at 2639.800 kb on - strand, within CCNA_02492 at 2639.800 kb on - strand, within CCNA_02492 at 2639.836 kb on - strand, within CCNA_02492 at 2639.838 kb on + strand, within CCNA_02492 at 2639.838 kb on + strand, within CCNA_02492 at 2639.846 kb on - strand, within CCNA_02492 at 2639.846 kb on - strand, within CCNA_02492 at 2639.853 kb on - strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.857 kb on + strand, within CCNA_02492 at 2639.865 kb on - strand, within CCNA_02492 at 2639.865 kb on - strand, within CCNA_02492 at 2639.888 kb on - strand, within CCNA_02492 at 2639.888 kb on - strand, within CCNA_02492 at 2639.920 kb on + strand, within CCNA_02492 at 2639.928 kb on - strand, within CCNA_02492 at 2639.930 kb on - strand, within CCNA_02492 at 2639.940 kb on + strand, within CCNA_02492 at 2640.002 kb on + strand, within CCNA_02492 at 2640.010 kb on - strand, within CCNA_02492 at 2640.010 kb on - strand, within CCNA_02492 at 2640.010 kb on - strand, within CCNA_02492 at 2640.010 kb on - strand, within CCNA_02492 at 2640.010 kb on - strand, within CCNA_02492 at 2640.025 kb on + strand, within CCNA_02492 at 2640.025 kb on + strand, within CCNA_02492 at 2640.165 kb on + strand, within CCNA_02492 at 2640.173 kb on - strand, within CCNA_02492 at 2640.224 kb on - strand, within CCNA_02492 at 2640.233 kb on + strand, within CCNA_02492 at 2640.241 kb on - strand, within CCNA_02492 at 2640.241 kb on - strand, within CCNA_02492 at 2640.267 kb on - strand at 2640.273 kb on - strand at 2640.318 kb on + strand at 2640.349 kb on - strand at 2640.349 kb on - strand at 2640.349 kb on - strand at 2640.349 kb on - strand at 2640.349 kb on - strand at 2640.349 kb on - strand at 2640.349 kb on - strand at 2640.404 kb on + strand at 2640.410 kb on + strand at 2640.410 kb on + strand at 2640.425 kb on + strand at 2640.425 kb on + strand at 2640.425 kb on + strand at 2640.425 kb on + strand at 2640.433 kb on - strand at 2640.433 kb on - strand at 2640.433 kb on - strand at 2640.451 kb on - strand at 2640.491 kb on + strand, within CCNA_02493 at 2640.521 kb on - strand, within CCNA_02493 at 2640.530 kb on + strand, within CCNA_02493 at 2640.538 kb on - strand, within CCNA_02493 at 2640.559 kb on + strand, within CCNA_02493 at 2640.562 kb on - strand, within CCNA_02493 at 2640.632 kb on + strand, within CCNA_02493 at 2640.632 kb on + strand, within CCNA_02493 at 2640.651 kb on + strand, within CCNA_02493 at 2640.651 kb on + strand, within CCNA_02493 at 2640.651 kb on + strand, within CCNA_02493 at 2640.659 kb on - strand, within CCNA_02493 at 2640.680 kb on - strand, within CCNA_02493 at 2640.686 kb on + strand, within CCNA_02493 at 2640.693 kb on - strand, within CCNA_02493 at 2640.704 kb on - strand, within CCNA_02493 at 2640.720 kb on - strand, within CCNA_02493 at 2640.771 kb on + strand, within CCNA_02493 at 2640.775 kb on - strand, within CCNA_02493 at 2640.779 kb on - strand, within CCNA_02493 at 2640.779 kb on - strand, within CCNA_02493 at 2640.781 kb on + strand, within CCNA_02493 at 2640.781 kb on - strand, within CCNA_02493 at 2640.803 kb on + strand, within CCNA_02493 at 2640.811 kb on - strand, within CCNA_02493 at 2640.816 kb on + strand, within CCNA_02493 at 2640.816 kb on - strand, within CCNA_02493 at 2640.860 kb on + strand, within CCNA_02493 at 2640.873 kb on - strand, within CCNA_02493 at 2640.891 kb on - strand, within CCNA_02493 at 2640.945 kb on - strand, within CCNA_02493 at 2640.986 kb on - strand, within CCNA_02493 at 2641.025 kb on + strand, within CCNA_02493 at 2641.025 kb on + strand, within CCNA_02493 at 2641.026 kb on + strand, within CCNA_02493 at 2641.033 kb on - strand, within CCNA_02493 at 2641.033 kb on - strand, within CCNA_02493 at 2641.041 kb on + strand, within CCNA_02493 at 2641.041 kb on + strand, within CCNA_02493 at 2641.057 kb on + strand, within CCNA_02493 at 2641.117 kb on + strand, within CCNA_02493 at 2641.125 kb on - strand, within CCNA_02493 at 2641.139 kb on + strand, within CCNA_02493 at 2641.147 kb on - strand, within CCNA_02493 at 2641.257 kb on + strand, within CCNA_02493 at 2641.265 kb on - strand, within CCNA_02493 at 2641.297 kb on + strand, within CCNA_02493 at 2641.302 kb on + strand, within CCNA_02493 at 2641.302 kb on + strand, within CCNA_02493 at 2641.302 kb on + strand, within CCNA_02493 at 2641.315 kb on - strand, within CCNA_02493 at 2641.328 kb on - strand, within CCNA_02493 at 2641.329 kb on - strand, within CCNA_02493 at 2641.330 kb on + strand, within CCNA_02493 at 2641.372 kb on - strand, within CCNA_02493 at 2641.379 kb on - strand at 2641.386 kb on - strand at 2641.397 kb on + strand at 2641.405 kb on - strand at 2641.420 kb on + strand at 2641.420 kb on + strand at 2641.437 kb on + strand at 2641.443 kb on - strand at 2641.458 kb on + strand at 2641.474 kb on - strand at 2641.476 kb on + strand at 2641.484 kb on - strand at 2641.497 kb on - strand at 2641.554 kb on + strand at 2641.607 kb on - strand at 2641.607 kb on - strand at 2641.625 kb on + strand, within CCNA_02494 at 2641.705 kb on - strand, within CCNA_02494 at 2641.720 kb on + strand, within CCNA_02494 at 2641.798 kb on + strand, within CCNA_02494 at 2641.806 kb on - strand, within CCNA_02494 at 2641.842 kb on + strand, within CCNA_02494 at 2641.844 kb on - strand, within CCNA_02494 at 2641.850 kb on - strand, within CCNA_02494 at 2641.850 kb on - strand, within CCNA_02494 at 2641.850 kb on - strand, within CCNA_02494 at 2641.858 kb on + strand, within CCNA_02494 at 2641.863 kb on + strand, within CCNA_02494 at 2641.891 kb on + strand, within CCNA_02494 at 2641.892 kb on - strand, within CCNA_02494 at 2641.899 kb on - strand, within CCNA_02494 at 2641.899 kb on - strand, within CCNA_02494 at 2641.918 kb on + strand, within CCNA_02494 at 2641.941 kb on - strand, within CCNA_02494 at 2641.962 kb on - strand, within CCNA_02494 at 2641.967 kb on - strand, within CCNA_02494 at 2641.982 kb on - strand, within CCNA_02494 at 2641.982 kb on - strand, within CCNA_02494 at 2641.991 kb on - strand, within CCNA_02494 at 2642.000 kb on + strand, within CCNA_02494 at 2642.008 kb on - strand, within CCNA_02494 at 2642.063 kb on - strand, within CCNA_02494 at 2642.075 kb on - strand, within CCNA_02494 at 2642.099 kb on + strand, within CCNA_02494 at 2642.122 kb on - strand, within CCNA_02494 at 2642.169 kb on - strand, within CCNA_02494 at 2642.172 kb on + strand, within CCNA_02494 at 2642.206 kb on + strand, within CCNA_02494 at 2642.214 kb on - strand, within CCNA_02494 at 2642.216 kb on + strand, within CCNA_02494 at 2642.216 kb on + strand, within CCNA_02494 at 2642.216 kb on + strand, within CCNA_02494 at 2642.216 kb on + strand, within CCNA_02494 at 2642.224 kb on - strand, within CCNA_02494 at 2642.224 kb on - strand, within CCNA_02494 at 2642.238 kb on + strand, within CCNA_02494 at 2642.238 kb on + strand, within CCNA_02494 at 2642.241 kb on - strand, within CCNA_02494 at 2642.269 kb on + strand, within CCNA_02494 at 2642.277 kb on - strand, within CCNA_02494 at 2642.279 kb on + strand, within CCNA_02494 at 2642.280 kb on - strand, within CCNA_02494 at 2642.295 kb on + strand, within CCNA_02494 at 2642.295 kb on + strand, within CCNA_02494 at 2642.303 kb on - strand, within CCNA_02494 at 2642.318 kb on - strand, within CCNA_02494 at 2642.318 kb on - strand at 2642.318 kb on - strand, within CCNA_02494 at 2642.318 kb on - strand, within CCNA_02494 at 2642.323 kb on + strand, within CCNA_02494 at 2642.339 kb on + strand, within CCNA_02494 at 2642.341 kb on + strand, within CCNA_02494 at 2642.341 kb on + strand, within CCNA_02494 at 2642.341 kb on + strand, within CCNA_02494 at 2642.341 kb on + strand, within CCNA_02494 at 2642.349 kb on - strand, within CCNA_02494 at 2642.349 kb on - strand, within CCNA_02494 at 2642.349 kb on - strand, within CCNA_02494 at 2642.349 kb on - strand, within CCNA_02494 at 2642.386 kb on + strand, within CCNA_02494 at 2642.441 kb on + strand, within CCNA_02494 at 2642.445 kb on - strand, within CCNA_02494 at 2642.452 kb on + strand, within CCNA_02494 at 2642.452 kb on + strand, within CCNA_02494 at 2642.452 kb on + strand, within CCNA_02494
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nickel (II) chloride 0.3 mM remove 2,639,362 - CCNA_02491 0.55 +0.3 2,639,393 + CCNA_02491 0.59 -1.2 2,639,465 - CCNA_02491 0.69 -0.2 2,639,484 + CCNA_02491 0.72 -0.5 2,639,492 - CCNA_02491 0.73 -1.0 2,639,492 - CCNA_02491 0.73 -0.6 2,639,562 + CCNA_02491 0.83 +0.0 2,639,562 + CCNA_02491 0.83 +1.4 2,639,597 + CCNA_02491 0.88 -0.3 2,639,600 + CCNA_02491 0.88 -1.2 2,639,605 - CCNA_02491 0.89 -0.1 2,639,605 - CCNA_02491 0.89 -1.5 2,639,608 - CCNA_02491 0.90 -0.3 2,639,612 + -1.8 2,639,612 + +1.2 2,639,634 - +0.0 2,639,639 - +0.4 2,639,641 + -0.2 2,639,645 + -0.6 2,639,653 - -0.0 2,639,668 + +1.6 2,639,698 - +0.9 2,639,725 - +0.5 2,639,761 + CCNA_02492 0.13 +0.1 2,639,776 - CCNA_02492 0.16 +0.5 2,639,786 - CCNA_02492 0.17 +2.1 2,639,786 - CCNA_02492 0.17 +0.3 2,639,795 - CCNA_02492 0.19 +0.1 2,639,800 - CCNA_02492 0.19 +0.3 2,639,800 - CCNA_02492 0.19 +1.4 2,639,800 - CCNA_02492 0.19 -0.4 2,639,836 - CCNA_02492 0.25 +1.1 2,639,838 + CCNA_02492 0.25 -0.2 2,639,838 + CCNA_02492 0.25 +1.1 2,639,846 - CCNA_02492 0.26 -0.0 2,639,846 - CCNA_02492 0.26 +0.6 2,639,853 - CCNA_02492 0.27 -0.0 2,639,857 + CCNA_02492 0.28 -0.6 2,639,857 + CCNA_02492 0.28 +1.5 2,639,857 + CCNA_02492 0.28 +0.3 2,639,857 + CCNA_02492 0.28 +0.1 2,639,857 + CCNA_02492 0.28 -0.6 2,639,857 + CCNA_02492 0.28 -1.4 2,639,857 + CCNA_02492 0.28 -0.5 2,639,865 - CCNA_02492 0.29 -0.9 2,639,865 - CCNA_02492 0.29 -0.7 2,639,888 - CCNA_02492 0.33 +0.9 2,639,888 - CCNA_02492 0.33 +1.2 2,639,920 + CCNA_02492 0.38 -1.4 2,639,928 - CCNA_02492 0.39 -0.9 2,639,930 - CCNA_02492 0.39 +0.3 2,639,940 + CCNA_02492 0.41 +0.8 2,640,002 + CCNA_02492 0.50 +0.3 2,640,010 - CCNA_02492 0.52 +1.1 2,640,010 - CCNA_02492 0.52 -0.2 2,640,010 - CCNA_02492 0.52 -0.8 2,640,010 - CCNA_02492 0.52 -2.5 2,640,010 - CCNA_02492 0.52 -0.7 2,640,025 + CCNA_02492 0.54 -0.4 2,640,025 + CCNA_02492 0.54 +0.3 2,640,165 + CCNA_02492 0.75 +0.6 2,640,173 - CCNA_02492 0.77 -0.7 2,640,224 - CCNA_02492 0.84 +1.1 2,640,233 + CCNA_02492 0.86 -0.0 2,640,241 - CCNA_02492 0.87 +1.1 2,640,241 - CCNA_02492 0.87 -0.7 2,640,267 - -0.0 2,640,273 - -0.2 2,640,318 + +0.8 2,640,349 - -0.4 2,640,349 - +1.1 2,640,349 - +1.6 2,640,349 - +0.6 2,640,349 - +0.4 2,640,349 - -0.5 2,640,349 - +0.1 2,640,404 + -0.9 2,640,410 + +0.6 2,640,410 + -1.1 2,640,425 + +1.9 2,640,425 + +1.8 2,640,425 + -1.2 2,640,425 + +1.5 2,640,433 - -1.3 2,640,433 - -1.7 2,640,433 - +0.5 2,640,451 - +1.4 2,640,491 + CCNA_02493 0.13 -0.6 2,640,521 - CCNA_02493 0.15 -0.2 2,640,530 + CCNA_02493 0.16 +1.4 2,640,538 - CCNA_02493 0.17 +0.5 2,640,559 + CCNA_02493 0.19 +1.2 2,640,562 - CCNA_02493 0.19 -0.6 2,640,632 + CCNA_02493 0.25 +1.1 2,640,632 + CCNA_02493 0.25 +0.3 2,640,651 + CCNA_02493 0.27 -1.8 2,640,651 + CCNA_02493 0.27 +0.1 2,640,651 + CCNA_02493 0.27 -1.4 2,640,659 - CCNA_02493 0.27 -1.2 2,640,680 - CCNA_02493 0.29 -2.6 2,640,686 + CCNA_02493 0.30 -0.8 2,640,693 - CCNA_02493 0.30 +0.1 2,640,704 - CCNA_02493 0.31 +0.4 2,640,720 - CCNA_02493 0.33 -1.4 2,640,771 + CCNA_02493 0.37 -0.0 2,640,775 - CCNA_02493 0.38 -0.6 2,640,779 - CCNA_02493 0.38 +1.4 2,640,779 - CCNA_02493 0.38 -0.5 2,640,781 + CCNA_02493 0.38 +1.2 2,640,781 - CCNA_02493 0.38 -0.1 2,640,803 + CCNA_02493 0.40 -0.2 2,640,811 - CCNA_02493 0.41 -0.2 2,640,816 + CCNA_02493 0.41 -1.0 2,640,816 - CCNA_02493 0.41 +0.6 2,640,860 + CCNA_02493 0.45 -0.3 2,640,873 - CCNA_02493 0.46 -0.1 2,640,891 - CCNA_02493 0.48 -1.9 2,640,945 - CCNA_02493 0.52 -0.8 2,640,986 - CCNA_02493 0.56 +0.1 2,641,025 + CCNA_02493 0.59 +1.2 2,641,025 + CCNA_02493 0.59 +0.1 2,641,026 + CCNA_02493 0.59 -1.0 2,641,033 - CCNA_02493 0.60 -0.5 2,641,033 - CCNA_02493 0.60 -0.5 2,641,041 + CCNA_02493 0.61 -1.1 2,641,041 + CCNA_02493 0.61 +0.5 2,641,057 + CCNA_02493 0.62 -0.4 2,641,117 + CCNA_02493 0.67 -0.2 2,641,125 - CCNA_02493 0.68 -1.2 2,641,139 + CCNA_02493 0.69 +0.1 2,641,147 - CCNA_02493 0.70 -1.2 2,641,257 + CCNA_02493 0.79 +1.9 2,641,265 - CCNA_02493 0.80 +0.2 2,641,297 + CCNA_02493 0.83 -1.5 2,641,302 + CCNA_02493 0.83 +1.2 2,641,302 + CCNA_02493 0.83 -0.9 2,641,302 + CCNA_02493 0.83 -0.7 2,641,315 - CCNA_02493 0.85 +1.4 2,641,328 - CCNA_02493 0.86 -0.3 2,641,329 - CCNA_02493 0.86 -0.1 2,641,330 + CCNA_02493 0.86 +0.1 2,641,372 - CCNA_02493 0.89 +0.1 2,641,379 - +0.5 2,641,386 - +0.4 2,641,397 + +0.7 2,641,405 - +0.8 2,641,420 + -0.7 2,641,420 + -0.5 2,641,437 + -0.4 2,641,443 - +0.1 2,641,458 + +0.6 2,641,474 - +0.8 2,641,476 + +0.4 2,641,484 - +0.1 2,641,497 - -0.3 2,641,554 + -0.0 2,641,607 - -0.3 2,641,607 - -1.5 2,641,625 + CCNA_02494 0.11 +0.5 2,641,705 - CCNA_02494 0.18 -0.6 2,641,720 + CCNA_02494 0.19 +0.5 2,641,798 + CCNA_02494 0.26 +1.2 2,641,806 - CCNA_02494 0.27 +1.4 2,641,842 + CCNA_02494 0.30 -0.2 2,641,844 - CCNA_02494 0.30 -0.4 2,641,850 - CCNA_02494 0.30 +0.8 2,641,850 - CCNA_02494 0.30 +1.9 2,641,850 - CCNA_02494 0.30 +0.1 2,641,858 + CCNA_02494 0.31 +0.4 2,641,863 + CCNA_02494 0.31 +0.5 2,641,891 + CCNA_02494 0.34 -0.3 2,641,892 - CCNA_02494 0.34 +0.4 2,641,899 - CCNA_02494 0.34 +0.8 2,641,899 - CCNA_02494 0.34 +0.9 2,641,918 + CCNA_02494 0.36 -0.4 2,641,941 - CCNA_02494 0.38 +1.6 2,641,962 - CCNA_02494 0.40 -0.7 2,641,967 - CCNA_02494 0.40 -1.1 2,641,982 - CCNA_02494 0.41 +1.1 2,641,982 - CCNA_02494 0.41 +1.1 2,641,991 - CCNA_02494 0.42 -0.1 2,642,000 + CCNA_02494 0.43 +1.6 2,642,008 - CCNA_02494 0.44 +0.8 2,642,063 - CCNA_02494 0.48 -0.5 2,642,075 - CCNA_02494 0.49 +0.8 2,642,099 + CCNA_02494 0.51 -0.0 2,642,122 - CCNA_02494 0.53 -0.3 2,642,169 - CCNA_02494 0.57 +0.6 2,642,172 + CCNA_02494 0.58 +0.7 2,642,206 + CCNA_02494 0.60 -0.2 2,642,214 - CCNA_02494 0.61 +0.5 2,642,216 + CCNA_02494 0.61 +0.1 2,642,216 + CCNA_02494 0.61 +0.9 2,642,216 + CCNA_02494 0.61 +0.2 2,642,216 + CCNA_02494 0.61 -0.9 2,642,224 - CCNA_02494 0.62 -1.2 2,642,224 - CCNA_02494 0.62 +0.3 2,642,238 + CCNA_02494 0.63 -0.4 2,642,238 + CCNA_02494 0.63 +0.9 2,642,241 - CCNA_02494 0.63 -0.9 2,642,269 + CCNA_02494 0.66 -1.1 2,642,277 - CCNA_02494 0.66 +2.2 2,642,279 + CCNA_02494 0.67 +0.4 2,642,280 - CCNA_02494 0.67 +0.6 2,642,295 + CCNA_02494 0.68 -0.8 2,642,295 + CCNA_02494 0.68 +1.9 2,642,303 - CCNA_02494 0.69 +0.9 2,642,318 - CCNA_02494 0.70 -0.2 2,642,318 - +2.2 2,642,318 - CCNA_02494 0.70 +1.1 2,642,318 - CCNA_02494 0.70 +0.8 2,642,323 + CCNA_02494 0.70 +1.2 2,642,339 + CCNA_02494 0.72 +0.3 2,642,341 + CCNA_02494 0.72 +1.2 2,642,341 + CCNA_02494 0.72 +2.2 2,642,341 + CCNA_02494 0.72 +1.1 2,642,341 + CCNA_02494 0.72 +0.9 2,642,349 - CCNA_02494 0.73 -0.8 2,642,349 - CCNA_02494 0.73 -1.6 2,642,349 - CCNA_02494 0.73 +1.1 2,642,349 - CCNA_02494 0.73 +1.9 2,642,386 + CCNA_02494 0.76 -0.1 2,642,441 + CCNA_02494 0.80 -0.6 2,642,445 - CCNA_02494 0.81 +2.9 2,642,452 + CCNA_02494 0.81 -0.2 2,642,452 + CCNA_02494 0.81 +0.1 2,642,452 + CCNA_02494 0.81 -1.0
Or see this region's nucleotide sequence