Experiment: PYE with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00118 and CCNA_00119 overlap by 13 nucleotides CCNA_00119 and CCNA_00120 overlap by 4 nucleotides CCNA_00120 and CCNA_00121 overlap by 4 nucleotides CCNA_00121 and CCNA_00122 are separated by 8 nucleotides
CCNA_00118: CCNA_00118 - porphyrin biosynthesis protein, at 129,746 to 131,989
_00118
CCNA_00119: CCNA_00119 - hypothetical protein, at 131,977 to 132,438
_00119
CCNA_00120: CCNA_00120 - putative acetyltransferase, at 132,435 to 132,953
_00120
CCNA_00121: CCNA_00121 - hypothetical protein, at 132,950 to 133,408
_00121
CCNA_00122: CCNA_00122 - hypothetical protein, at 133,417 to 133,827
_00122
Position (kb) 131
132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 131.101 kb on + strand, within CCNA_00118 at 131.111 kb on - strand, within CCNA_00118 at 131.114 kb on + strand, within CCNA_00118 at 131.122 kb on - strand, within CCNA_00118 at 131.124 kb on + strand, within CCNA_00118 at 131.246 kb on + strand, within CCNA_00118 at 131.246 kb on + strand, within CCNA_00118 at 131.254 kb on - strand, within CCNA_00118 at 131.264 kb on - strand, within CCNA_00118 at 131.294 kb on + strand, within CCNA_00118 at 131.306 kb on + strand, within CCNA_00118 at 131.313 kb on - strand, within CCNA_00118 at 131.319 kb on - strand, within CCNA_00118 at 131.322 kb on + strand, within CCNA_00118 at 131.325 kb on - strand, within CCNA_00118 at 131.330 kb on - strand, within CCNA_00118 at 131.357 kb on - strand, within CCNA_00118 at 131.442 kb on + strand, within CCNA_00118 at 131.450 kb on - strand, within CCNA_00118 at 131.482 kb on + strand, within CCNA_00118 at 131.487 kb on + strand, within CCNA_00118 at 131.490 kb on - strand, within CCNA_00118 at 131.490 kb on - strand, within CCNA_00118 at 131.490 kb on - strand, within CCNA_00118 at 131.495 kb on - strand, within CCNA_00118 at 131.496 kb on - strand, within CCNA_00118 at 131.525 kb on + strand, within CCNA_00118 at 131.533 kb on - strand, within CCNA_00118 at 131.609 kb on + strand, within CCNA_00118 at 131.630 kb on + strand, within CCNA_00118 at 131.640 kb on - strand, within CCNA_00118 at 131.642 kb on + strand, within CCNA_00118 at 131.669 kb on + strand, within CCNA_00118 at 131.684 kb on - strand, within CCNA_00118 at 131.709 kb on - strand, within CCNA_00118 at 131.709 kb on - strand, within CCNA_00118 at 131.755 kb on - strand, within CCNA_00118 at 131.777 kb on + strand at 131.790 kb on - strand at 131.790 kb on - strand at 131.800 kb on + strand at 131.808 kb on - strand at 131.837 kb on + strand at 131.847 kb on + strand at 131.867 kb on + strand at 131.923 kb on + strand at 131.923 kb on + strand at 131.931 kb on - strand at 131.931 kb on - strand at 131.931 kb on - strand at 131.939 kb on + strand at 131.947 kb on - strand at 131.947 kb on - strand at 131.950 kb on + strand at 131.962 kb on + strand at 131.967 kb on + strand at 131.970 kb on - strand at 132.006 kb on + strand at 132.006 kb on + strand at 132.006 kb on + strand at 132.007 kb on + strand at 132.014 kb on - strand at 132.014 kb on - strand at 132.025 kb on - strand, within CCNA_00119 at 132.054 kb on - strand, within CCNA_00119 at 132.082 kb on + strand, within CCNA_00119 at 132.092 kb on - strand, within CCNA_00119 at 132.097 kb on + strand, within CCNA_00119 at 132.097 kb on + strand, within CCNA_00119 at 132.097 kb on + strand, within CCNA_00119 at 132.097 kb on + strand, within CCNA_00119 at 132.106 kb on + strand, within CCNA_00119 at 132.106 kb on + strand, within CCNA_00119 at 132.106 kb on + strand, within CCNA_00119 at 132.106 kb on + strand, within CCNA_00119 at 132.106 kb on + strand, within CCNA_00119 at 132.106 kb on + strand, within CCNA_00119 at 132.114 kb on - strand, within CCNA_00119 at 132.114 kb on - strand, within CCNA_00119 at 132.142 kb on + strand, within CCNA_00119 at 132.161 kb on - strand, within CCNA_00119 at 132.199 kb on + strand, within CCNA_00119 at 132.207 kb on - strand, within CCNA_00119 at 132.232 kb on - strand, within CCNA_00119 at 132.232 kb on - strand, within CCNA_00119 at 132.263 kb on + strand, within CCNA_00119 at 132.297 kb on - strand, within CCNA_00119 at 132.302 kb on + strand, within CCNA_00119 at 132.310 kb on - strand, within CCNA_00119 at 132.317 kb on + strand, within CCNA_00119 at 132.325 kb on - strand, within CCNA_00119 at 132.325 kb on - strand, within CCNA_00119 at 132.335 kb on + strand, within CCNA_00119 at 132.358 kb on - strand, within CCNA_00119 at 132.371 kb on + strand, within CCNA_00119 at 132.371 kb on + strand, within CCNA_00119 at 132.371 kb on + strand, within CCNA_00119 at 132.371 kb on + strand, within CCNA_00119 at 132.371 kb on + strand, within CCNA_00119 at 132.407 kb on + strand at 132.429 kb on + strand at 132.429 kb on + strand at 132.429 kb on + strand at 132.429 kb on + strand at 132.429 kb on + strand at 132.429 kb on + strand at 132.429 kb on + strand at 132.432 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.437 kb on - strand at 132.503 kb on + strand, within CCNA_00120 at 132.503 kb on + strand, within CCNA_00120 at 132.510 kb on - strand, within CCNA_00120 at 132.511 kb on - strand, within CCNA_00120 at 132.520 kb on + strand, within CCNA_00120 at 132.525 kb on + strand, within CCNA_00120 at 132.527 kb on + strand, within CCNA_00120 at 132.528 kb on - strand, within CCNA_00120 at 132.535 kb on - strand, within CCNA_00120 at 132.546 kb on + strand, within CCNA_00120 at 132.577 kb on + strand, within CCNA_00120 at 132.577 kb on + strand, within CCNA_00120 at 132.577 kb on + strand, within CCNA_00120 at 132.594 kb on - strand, within CCNA_00120 at 132.649 kb on - strand, within CCNA_00120 at 132.661 kb on + strand, within CCNA_00120 at 132.671 kb on + strand, within CCNA_00120 at 132.674 kb on - strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.691 kb on + strand, within CCNA_00120 at 132.697 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.699 kb on - strand, within CCNA_00120 at 132.752 kb on - strand, within CCNA_00120 at 132.759 kb on + strand, within CCNA_00120 at 132.762 kb on - strand, within CCNA_00120 at 132.762 kb on - strand, within CCNA_00120 at 132.762 kb on - strand, within CCNA_00120 at 132.762 kb on - strand, within CCNA_00120 at 132.791 kb on + strand, within CCNA_00120 at 132.799 kb on - strand, within CCNA_00120 at 132.799 kb on - strand, within CCNA_00120 at 132.799 kb on - strand, within CCNA_00120 at 132.799 kb on - strand, within CCNA_00120 at 132.852 kb on - strand, within CCNA_00120 at 132.861 kb on + strand, within CCNA_00120 at 132.869 kb on - strand, within CCNA_00120 at 132.869 kb on - strand, within CCNA_00120 at 132.871 kb on + strand, within CCNA_00120 at 132.879 kb on - strand, within CCNA_00120 at 132.879 kb on - strand, within CCNA_00120 at 132.925 kb on + strand at 132.930 kb on + strand at 132.930 kb on + strand at 132.930 kb on + strand at 132.933 kb on - strand at 132.938 kb on - strand at 132.938 kb on - strand at 132.964 kb on + strand at 132.974 kb on + strand at 132.994 kb on + strand at 133.026 kb on + strand, within CCNA_00121 at 133.026 kb on + strand, within CCNA_00121 at 133.026 kb on + strand, within CCNA_00121 at 133.029 kb on - strand, within CCNA_00121 at 133.030 kb on + strand, within CCNA_00121 at 133.034 kb on - strand, within CCNA_00121 at 133.034 kb on - strand, within CCNA_00121 at 133.034 kb on - strand, within CCNA_00121 at 133.044 kb on - strand, within CCNA_00121 at 133.045 kb on - strand, within CCNA_00121 at 133.053 kb on - strand, within CCNA_00121 at 133.073 kb on - strand, within CCNA_00121 at 133.113 kb on + strand, within CCNA_00121 at 133.151 kb on + strand, within CCNA_00121 at 133.164 kb on - strand, within CCNA_00121 at 133.169 kb on - strand, within CCNA_00121 at 133.186 kb on + strand, within CCNA_00121 at 133.215 kb on - strand, within CCNA_00121 at 133.254 kb on - strand, within CCNA_00121 at 133.259 kb on - strand, within CCNA_00121 at 133.259 kb on - strand, within CCNA_00121 at 133.261 kb on + strand, within CCNA_00121 at 133.261 kb on + strand, within CCNA_00121 at 133.261 kb on + strand, within CCNA_00121 at 133.261 kb on - strand, within CCNA_00121 at 133.261 kb on - strand, within CCNA_00121 at 133.313 kb on + strand, within CCNA_00121 at 133.313 kb on + strand, within CCNA_00121 at 133.321 kb on - strand, within CCNA_00121 at 133.321 kb on - strand, within CCNA_00121 at 133.321 kb on - strand, within CCNA_00121 at 133.321 kb on - strand, within CCNA_00121 at 133.321 kb on - strand, within CCNA_00121 at 133.335 kb on + strand, within CCNA_00121 at 133.335 kb on + strand, within CCNA_00121 at 133.343 kb on - strand, within CCNA_00121 at 133.343 kb on - strand, within CCNA_00121 at 133.343 kb on - strand, within CCNA_00121 at 133.343 kb on - strand, within CCNA_00121 at 133.362 kb on + strand, within CCNA_00121 at 133.362 kb on + strand, within CCNA_00121 at 133.370 kb on + strand at 133.370 kb on - strand at 133.378 kb on - strand at 133.383 kb on - strand at 133.406 kb on - strand at 133.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nickel (II) chloride 0.3 mM remove 131,101 + CCNA_00118 0.60 +1.2 131,111 - CCNA_00118 0.61 -1.8 131,114 + CCNA_00118 0.61 +0.1 131,122 - CCNA_00118 0.61 -1.7 131,124 + CCNA_00118 0.61 -0.5 131,246 + CCNA_00118 0.67 +0.0 131,246 + CCNA_00118 0.67 -2.0 131,254 - CCNA_00118 0.67 -0.8 131,264 - CCNA_00118 0.68 +0.9 131,294 + CCNA_00118 0.69 -0.1 131,306 + CCNA_00118 0.70 -1.2 131,313 - CCNA_00118 0.70 +2.2 131,319 - CCNA_00118 0.70 +1.2 131,322 + CCNA_00118 0.70 -0.6 131,325 - CCNA_00118 0.70 -0.5 131,330 - CCNA_00118 0.71 +0.6 131,357 - CCNA_00118 0.72 +0.1 131,442 + CCNA_00118 0.76 +0.1 131,450 - CCNA_00118 0.76 -0.4 131,482 + CCNA_00118 0.77 -0.1 131,487 + CCNA_00118 0.78 -1.4 131,490 - CCNA_00118 0.78 +0.8 131,490 - CCNA_00118 0.78 -1.5 131,490 - CCNA_00118 0.78 -0.2 131,495 - CCNA_00118 0.78 +1.9 131,496 - CCNA_00118 0.78 +0.4 131,525 + CCNA_00118 0.79 -1.9 131,533 - CCNA_00118 0.80 +0.2 131,609 + CCNA_00118 0.83 +2.6 131,630 + CCNA_00118 0.84 -1.7 131,640 - CCNA_00118 0.84 +2.2 131,642 + CCNA_00118 0.84 -0.0 131,669 + CCNA_00118 0.86 -1.0 131,684 - CCNA_00118 0.86 +0.5 131,709 - CCNA_00118 0.87 -1.7 131,709 - CCNA_00118 0.87 -0.6 131,755 - CCNA_00118 0.90 +1.2 131,777 + -0.1 131,790 - -0.3 131,790 - +0.5 131,800 + +1.6 131,808 - -1.0 131,837 + +5.6 131,847 + -1.0 131,867 + -0.4 131,923 + -1.1 131,923 + +2.1 131,931 - -0.6 131,931 - +0.5 131,931 - +1.4 131,939 + +0.8 131,947 - -1.7 131,947 - +2.0 131,950 + -0.4 131,962 + +1.3 131,967 + -1.0 131,970 - +0.5 132,006 + -0.4 132,006 + -1.3 132,006 + +0.0 132,007 + +3.2 132,014 - +1.4 132,014 - +1.2 132,025 - CCNA_00119 0.10 -0.1 132,054 - CCNA_00119 0.17 +0.6 132,082 + CCNA_00119 0.23 +0.8 132,092 - CCNA_00119 0.25 -2.1 132,097 + CCNA_00119 0.26 -0.2 132,097 + CCNA_00119 0.26 -1.1 132,097 + CCNA_00119 0.26 -0.7 132,097 + CCNA_00119 0.26 -0.4 132,106 + CCNA_00119 0.28 -1.6 132,106 + CCNA_00119 0.28 +0.7 132,106 + CCNA_00119 0.28 -1.8 132,106 + CCNA_00119 0.28 -0.4 132,106 + CCNA_00119 0.28 -1.1 132,106 + CCNA_00119 0.28 -1.9 132,114 - CCNA_00119 0.30 +0.9 132,114 - CCNA_00119 0.30 -0.4 132,142 + CCNA_00119 0.36 -2.1 132,161 - CCNA_00119 0.40 +0.7 132,199 + CCNA_00119 0.48 -0.5 132,207 - CCNA_00119 0.50 +0.9 132,232 - CCNA_00119 0.55 -0.2 132,232 - CCNA_00119 0.55 +2.2 132,263 + CCNA_00119 0.62 -0.0 132,297 - CCNA_00119 0.69 +0.4 132,302 + CCNA_00119 0.70 -1.6 132,310 - CCNA_00119 0.72 -1.4 132,317 + CCNA_00119 0.74 -2.7 132,325 - CCNA_00119 0.75 -1.0 132,325 - CCNA_00119 0.75 +1.2 132,335 + CCNA_00119 0.77 +0.3 132,358 - CCNA_00119 0.82 -0.7 132,371 + CCNA_00119 0.85 +0.2 132,371 + CCNA_00119 0.85 -1.8 132,371 + CCNA_00119 0.85 -1.5 132,371 + CCNA_00119 0.85 -1.0 132,371 + CCNA_00119 0.85 -1.4 132,407 + -0.6 132,429 + -0.5 132,429 + +0.7 132,429 + -1.4 132,429 + +0.9 132,429 + -2.9 132,429 + -1.6 132,429 + +0.4 132,432 - +0.9 132,437 - +1.4 132,437 - +0.3 132,437 - -0.1 132,437 - -0.6 132,437 - +0.4 132,437 - -1.2 132,437 - +0.3 132,437 - +1.2 132,437 - +0.5 132,437 - +0.2 132,503 + CCNA_00120 0.13 +1.2 132,503 + CCNA_00120 0.13 -0.8 132,510 - CCNA_00120 0.14 -0.9 132,511 - CCNA_00120 0.15 -1.4 132,520 + CCNA_00120 0.16 -0.6 132,525 + CCNA_00120 0.17 -1.6 132,527 + CCNA_00120 0.18 -1.7 132,528 - CCNA_00120 0.18 +1.2 132,535 - CCNA_00120 0.19 -1.3 132,546 + CCNA_00120 0.21 +1.2 132,577 + CCNA_00120 0.27 +0.3 132,577 + CCNA_00120 0.27 -0.5 132,577 + CCNA_00120 0.27 -1.1 132,594 - CCNA_00120 0.31 +1.4 132,649 - CCNA_00120 0.41 +1.9 132,661 + CCNA_00120 0.44 -0.3 132,671 + CCNA_00120 0.45 -1.1 132,674 - CCNA_00120 0.46 +0.5 132,691 + CCNA_00120 0.49 -1.3 132,691 + CCNA_00120 0.49 +1.1 132,691 + CCNA_00120 0.49 -0.8 132,691 + CCNA_00120 0.49 +0.0 132,691 + CCNA_00120 0.49 -0.7 132,691 + CCNA_00120 0.49 +0.1 132,691 + CCNA_00120 0.49 -1.0 132,691 + CCNA_00120 0.49 -3.1 132,691 + CCNA_00120 0.49 -0.8 132,697 - CCNA_00120 0.50 -0.9 132,699 - CCNA_00120 0.51 +1.5 132,699 - CCNA_00120 0.51 -0.4 132,699 - CCNA_00120 0.51 -0.1 132,699 - CCNA_00120 0.51 +1.0 132,699 - CCNA_00120 0.51 +0.2 132,699 - CCNA_00120 0.51 -0.8 132,699 - CCNA_00120 0.51 -1.7 132,699 - CCNA_00120 0.51 -1.9 132,699 - CCNA_00120 0.51 +1.3 132,699 - CCNA_00120 0.51 -0.2 132,699 - CCNA_00120 0.51 -1.8 132,752 - CCNA_00120 0.61 +0.1 132,759 + CCNA_00120 0.62 -1.1 132,762 - CCNA_00120 0.63 +0.7 132,762 - CCNA_00120 0.63 +0.4 132,762 - CCNA_00120 0.63 -1.5 132,762 - CCNA_00120 0.63 -0.1 132,791 + CCNA_00120 0.69 -1.3 132,799 - CCNA_00120 0.70 +0.8 132,799 - CCNA_00120 0.70 +0.6 132,799 - CCNA_00120 0.70 +0.0 132,799 - CCNA_00120 0.70 +0.1 132,852 - CCNA_00120 0.80 +1.0 132,861 + CCNA_00120 0.82 -0.5 132,869 - CCNA_00120 0.84 -1.4 132,869 - CCNA_00120 0.84 -0.1 132,871 + CCNA_00120 0.84 -0.9 132,879 - CCNA_00120 0.86 -1.2 132,879 - CCNA_00120 0.86 -1.2 132,925 + -0.2 132,930 + +0.5 132,930 + +0.6 132,930 + -0.2 132,933 - +0.2 132,938 - +0.2 132,938 - +0.6 132,964 + +0.2 132,974 + +0.9 132,994 + +0.4 133,026 + CCNA_00121 0.17 -1.3 133,026 + CCNA_00121 0.17 +1.6 133,026 + CCNA_00121 0.17 -1.0 133,029 - CCNA_00121 0.17 -0.1 133,030 + CCNA_00121 0.17 -0.1 133,034 - CCNA_00121 0.18 +0.9 133,034 - CCNA_00121 0.18 -1.0 133,034 - CCNA_00121 0.18 +0.1 133,044 - CCNA_00121 0.20 -1.0 133,045 - CCNA_00121 0.21 -0.1 133,053 - CCNA_00121 0.22 -0.3 133,073 - CCNA_00121 0.27 -0.8 133,113 + CCNA_00121 0.36 -1.2 133,151 + CCNA_00121 0.44 -1.3 133,164 - CCNA_00121 0.47 -0.0 133,169 - CCNA_00121 0.48 -0.7 133,186 + CCNA_00121 0.51 +0.5 133,215 - CCNA_00121 0.58 -0.0 133,254 - CCNA_00121 0.66 -1.8 133,259 - CCNA_00121 0.67 +0.2 133,259 - CCNA_00121 0.67 -0.3 133,261 + CCNA_00121 0.68 -0.7 133,261 + CCNA_00121 0.68 -0.4 133,261 + CCNA_00121 0.68 +0.1 133,261 - CCNA_00121 0.68 +0.3 133,261 - CCNA_00121 0.68 +0.1 133,313 + CCNA_00121 0.79 -0.3 133,313 + CCNA_00121 0.79 +0.5 133,321 - CCNA_00121 0.81 -0.9 133,321 - CCNA_00121 0.81 +0.5 133,321 - CCNA_00121 0.81 +1.4 133,321 - CCNA_00121 0.81 +1.4 133,321 - CCNA_00121 0.81 -1.4 133,335 + CCNA_00121 0.84 +0.3 133,335 + CCNA_00121 0.84 -0.5 133,343 - CCNA_00121 0.86 -0.9 133,343 - CCNA_00121 0.86 +0.0 133,343 - CCNA_00121 0.86 +1.9 133,343 - CCNA_00121 0.86 +0.3 133,362 + CCNA_00121 0.90 -0.2 133,362 + CCNA_00121 0.90 -0.9 133,370 + -0.5 133,370 - +1.9 133,378 - -2.3 133,383 - +0.6 133,406 - -0.3 133,426 + +0.3
Or see this region's nucleotide sequence