Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2993 and Rru_A2994 overlap by 4 nucleotides Rru_A2994 and Rru_A2995 overlap by 4 nucleotides Rru_A2995 and Rru_A2996 are separated by 126 nucleotides
Rru_A2993: Rru_A2993 - hypothetical protein (NCBI), at 3,445,396 to 3,446,841
_A2993
Rru_A2994: Rru_A2994 - hypothetical protein (NCBI), at 3,446,838 to 3,448,145
_A2994
Rru_A2995: Rru_A2995 - hypothetical protein (NCBI), at 3,448,142 to 3,448,555
_A2995
Rru_A2996: Rru_A2996 - Cobalamin (vitamin B12) biosynthesis CbiD protein (NCBI), at 3,448,682 to 3,449,755
_A2996
Position (kb)
3446
3447
3448
3449 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3445.889 kb on + strand, within Rru_A2993 at 3445.889 kb on + strand, within Rru_A2993 at 3445.889 kb on + strand, within Rru_A2993 at 3445.890 kb on - strand, within Rru_A2993 at 3446.029 kb on + strand, within Rru_A2993 at 3446.037 kb on + strand, within Rru_A2993 at 3446.050 kb on - strand, within Rru_A2993 at 3446.080 kb on - strand, within Rru_A2993 at 3446.324 kb on - strand, within Rru_A2993 at 3446.445 kb on + strand, within Rru_A2993 at 3446.445 kb on + strand, within Rru_A2993 at 3446.445 kb on + strand, within Rru_A2993 at 3446.450 kb on - strand, within Rru_A2993 at 3446.450 kb on - strand, within Rru_A2993 at 3446.501 kb on + strand, within Rru_A2993 at 3446.502 kb on - strand, within Rru_A2993 at 3446.502 kb on - strand, within Rru_A2993 at 3446.502 kb on - strand, within Rru_A2993 at 3446.604 kb on + strand, within Rru_A2993 at 3446.604 kb on + strand, within Rru_A2993 at 3446.647 kb on + strand, within Rru_A2993 at 3446.647 kb on + strand, within Rru_A2993 at 3446.647 kb on + strand, within Rru_A2993 at 3446.647 kb on + strand, within Rru_A2993 at 3446.647 kb on + strand, within Rru_A2993 at 3446.727 kb on + strand at 3446.728 kb on - strand at 3446.728 kb on - strand at 3446.728 kb on - strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.747 kb on + strand at 3446.748 kb on - strand at 3446.758 kb on + strand at 3446.767 kb on + strand at 3446.777 kb on + strand at 3447.406 kb on - strand, within Rru_A2994 at 3447.445 kb on - strand, within Rru_A2994 at 3447.503 kb on - strand, within Rru_A2994 at 3447.503 kb on - strand, within Rru_A2994 at 3447.550 kb on - strand, within Rru_A2994 at 3448.011 kb on - strand, within Rru_A2994 at 3448.089 kb on - strand at 3448.217 kb on - strand, within Rru_A2995 at 3448.218 kb on + strand, within Rru_A2995 at 3448.218 kb on + strand, within Rru_A2995 at 3448.218 kb on + strand, within Rru_A2995 at 3448.219 kb on - strand, within Rru_A2995 at 3448.248 kb on + strand, within Rru_A2995 at 3448.248 kb on + strand, within Rru_A2995 at 3448.248 kb on + strand, within Rru_A2995 at 3448.249 kb on - strand, within Rru_A2995 at 3448.259 kb on + strand, within Rru_A2995 at 3448.260 kb on - strand, within Rru_A2995 at 3448.260 kb on - strand, within Rru_A2995 at 3448.261 kb on + strand, within Rru_A2995 at 3448.262 kb on - strand, within Rru_A2995 at 3448.302 kb on + strand, within Rru_A2995 at 3448.302 kb on + strand, within Rru_A2995 at 3448.302 kb on + strand, within Rru_A2995 at 3448.303 kb on - strand, within Rru_A2995 at 3448.303 kb on - strand, within Rru_A2995 at 3448.303 kb on - strand, within Rru_A2995 at 3448.303 kb on - strand, within Rru_A2995 at 3448.303 kb on - strand, within Rru_A2995 at 3448.303 kb on - strand, within Rru_A2995 at 3448.333 kb on - strand, within Rru_A2995 at 3448.341 kb on + strand, within Rru_A2995 at 3448.363 kb on + strand, within Rru_A2995 at 3448.363 kb on + strand, within Rru_A2995 at 3448.363 kb on + strand, within Rru_A2995 at 3448.364 kb on - strand, within Rru_A2995 at 3448.364 kb on - strand, within Rru_A2995 at 3448.364 kb on - strand, within Rru_A2995 at 3448.591 kb on + strand at 3448.596 kb on + strand at 3448.662 kb on + strand at 3448.663 kb on - strand at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.005 kb on + strand, within Rru_A2996 at 3449.006 kb on - strand, within Rru_A2996 at 3449.006 kb on - strand, within Rru_A2996 at 3449.006 kb on - strand, within Rru_A2996 at 3449.035 kb on - strand, within Rru_A2996
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 3,445,889 + Rru_A2993 0.34 -1.0 3,445,889 + Rru_A2993 0.34 -0.0 3,445,889 + Rru_A2993 0.34 -1.3 3,445,890 - Rru_A2993 0.34 +0.5 3,446,029 + Rru_A2993 0.44 +0.4 3,446,037 + Rru_A2993 0.44 -0.7 3,446,050 - Rru_A2993 0.45 -0.4 3,446,080 - Rru_A2993 0.47 +0.1 3,446,324 - Rru_A2993 0.64 -3.9 3,446,445 + Rru_A2993 0.73 +0.9 3,446,445 + Rru_A2993 0.73 -0.0 3,446,445 + Rru_A2993 0.73 -1.9 3,446,450 - Rru_A2993 0.73 -0.1 3,446,450 - Rru_A2993 0.73 -2.1 3,446,501 + Rru_A2993 0.76 +0.8 3,446,502 - Rru_A2993 0.76 +0.1 3,446,502 - Rru_A2993 0.76 +0.0 3,446,502 - Rru_A2993 0.76 +0.4 3,446,604 + Rru_A2993 0.84 +0.3 3,446,604 + Rru_A2993 0.84 +0.1 3,446,647 + Rru_A2993 0.87 -1.2 3,446,647 + Rru_A2993 0.87 -0.9 3,446,647 + Rru_A2993 0.87 -0.3 3,446,647 + Rru_A2993 0.87 -0.2 3,446,647 + Rru_A2993 0.87 +0.8 3,446,727 + +0.3 3,446,728 - +0.5 3,446,728 - +1.8 3,446,728 - +1.2 3,446,747 + -0.4 3,446,747 + +0.2 3,446,747 + +0.7 3,446,747 + -0.0 3,446,747 + +0.9 3,446,747 + +1.0 3,446,747 + +1.1 3,446,747 + -0.7 3,446,748 - +0.8 3,446,758 + -0.7 3,446,767 + -1.6 3,446,777 + -1.9 3,447,406 - Rru_A2994 0.43 -0.4 3,447,445 - Rru_A2994 0.46 -1.3 3,447,503 - Rru_A2994 0.51 +0.3 3,447,503 - Rru_A2994 0.51 -1.3 3,447,550 - Rru_A2994 0.54 -3.3 3,448,011 - Rru_A2994 0.90 -0.0 3,448,089 - +0.4 3,448,217 - Rru_A2995 0.18 -0.0 3,448,218 + Rru_A2995 0.18 -0.1 3,448,218 + Rru_A2995 0.18 -0.2 3,448,218 + Rru_A2995 0.18 -2.2 3,448,219 - Rru_A2995 0.19 -0.1 3,448,248 + Rru_A2995 0.26 +0.6 3,448,248 + Rru_A2995 0.26 -0.9 3,448,248 + Rru_A2995 0.26 -0.1 3,448,249 - Rru_A2995 0.26 -0.1 3,448,259 + Rru_A2995 0.28 -0.0 3,448,260 - Rru_A2995 0.29 -1.3 3,448,260 - Rru_A2995 0.29 -1.0 3,448,261 + Rru_A2995 0.29 +0.3 3,448,262 - Rru_A2995 0.29 -0.3 3,448,302 + Rru_A2995 0.39 +0.9 3,448,302 + Rru_A2995 0.39 -0.1 3,448,302 + Rru_A2995 0.39 +0.1 3,448,303 - Rru_A2995 0.39 -0.4 3,448,303 - Rru_A2995 0.39 -0.0 3,448,303 - Rru_A2995 0.39 -1.0 3,448,303 - Rru_A2995 0.39 +0.2 3,448,303 - Rru_A2995 0.39 +0.5 3,448,303 - Rru_A2995 0.39 +0.4 3,448,333 - Rru_A2995 0.46 -1.7 3,448,341 + Rru_A2995 0.48 +0.2 3,448,363 + Rru_A2995 0.53 +0.1 3,448,363 + Rru_A2995 0.53 -0.1 3,448,363 + Rru_A2995 0.53 -3.4 3,448,364 - Rru_A2995 0.54 +0.1 3,448,364 - Rru_A2995 0.54 -2.8 3,448,364 - Rru_A2995 0.54 -0.9 3,448,591 + +0.3 3,448,596 + +0.1 3,448,662 + -0.0 3,448,663 - +1.7 3,449,005 + Rru_A2996 0.30 -0.5 3,449,005 + Rru_A2996 0.30 +0.1 3,449,005 + Rru_A2996 0.30 -0.4 3,449,005 + Rru_A2996 0.30 -0.2 3,449,005 + Rru_A2996 0.30 -0.1 3,449,005 + Rru_A2996 0.30 -0.1 3,449,005 + Rru_A2996 0.30 -1.3 3,449,005 + Rru_A2996 0.30 -0.5 3,449,005 + Rru_A2996 0.30 +0.5 3,449,005 + Rru_A2996 0.30 +0.4 3,449,005 + Rru_A2996 0.30 +0.0 3,449,005 + Rru_A2996 0.30 +0.3 3,449,006 - Rru_A2996 0.30 +0.0 3,449,006 - Rru_A2996 0.30 +1.1 3,449,006 - Rru_A2996 0.30 -0.1 3,449,035 - Rru_A2996 0.33 +0.3
Or see this region's nucleotide sequence