Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0320 and Rru_A0321 are separated by 19 nucleotides Rru_A0321 and Rru_A0322 are separated by 9 nucleotides Rru_A0322 and Rru_A0323 are separated by 37 nucleotides Rru_A0323 and Rru_A0324 are separated by 39 nucleotides
Rru_A0320: Rru_A0320 - NADH dehydrogenase (quinone) (NCBI), at 389,490 to 391,085
_A0320
Rru_A0321: Rru_A0321 - NADH dehydrogenase (ubiquinone), 20 kDa subunit (NCBI), at 391,105 to 391,626
_A0321
Rru_A0322: Rru_A0322 - Peptidase M52, hydrogen uptake protein (NCBI), at 391,636 to 392,100
_A0322
Rru_A0323: Rru_A0323 - 4Fe-4S ferredoxin, iron-sulfur binding (NCBI), at 392,138 to 392,668
_A0323
Rru_A0324: Rru_A0324 - Formate dehydrogenase, alpha subunit (NCBI), at 392,708 to 394,849
_A0324
Position (kb) 391
392
393 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 390.696 kb on + strand, within Rru_A0320 at 390.696 kb on + strand, within Rru_A0320 at 390.696 kb on + strand, within Rru_A0320 at 390.696 kb on + strand, within Rru_A0320 at 390.790 kb on - strand, within Rru_A0320 at 391.084 kb on - strand at 391.114 kb on + strand at 391.115 kb on - strand at 391.222 kb on + strand, within Rru_A0321 at 391.223 kb on - strand, within Rru_A0321 at 391.223 kb on - strand, within Rru_A0321 at 391.225 kb on + strand, within Rru_A0321 at 391.225 kb on + strand, within Rru_A0321 at 391.226 kb on - strand, within Rru_A0321 at 391.226 kb on - strand, within Rru_A0321 at 391.336 kb on + strand, within Rru_A0321 at 391.337 kb on - strand, within Rru_A0321 at 391.414 kb on + strand, within Rru_A0321 at 391.414 kb on + strand, within Rru_A0321 at 391.415 kb on - strand, within Rru_A0321 at 391.415 kb on - strand, within Rru_A0321 at 391.415 kb on - strand, within Rru_A0321 at 391.459 kb on + strand, within Rru_A0321 at 391.459 kb on + strand, within Rru_A0321 at 391.459 kb on + strand, within Rru_A0321 at 391.460 kb on - strand, within Rru_A0321 at 391.460 kb on - strand, within Rru_A0321 at 391.460 kb on - strand, within Rru_A0321 at 391.460 kb on - strand, within Rru_A0321 at 391.460 kb on - strand, within Rru_A0321 at 391.547 kb on - strand, within Rru_A0321 at 391.547 kb on - strand, within Rru_A0321 at 391.547 kb on - strand, within Rru_A0321 at 391.791 kb on - strand, within Rru_A0322 at 391.791 kb on - strand, within Rru_A0322 at 391.801 kb on + strand, within Rru_A0322 at 391.802 kb on - strand, within Rru_A0322 at 391.802 kb on - strand, within Rru_A0322 at 391.802 kb on - strand, within Rru_A0322 at 391.960 kb on + strand, within Rru_A0322 at 391.961 kb on - strand, within Rru_A0322 at 391.961 kb on - strand, within Rru_A0322 at 392.078 kb on + strand at 392.078 kb on + strand at 392.079 kb on - strand at 392.079 kb on - strand at 392.079 kb on - strand at 392.370 kb on - strand, within Rru_A0323 at 392.512 kb on + strand, within Rru_A0323 at 392.513 kb on - strand, within Rru_A0323 at 392.555 kb on - strand, within Rru_A0323 at 392.700 kb on + strand at 392.700 kb on + strand at 392.735 kb on + strand at 392.837 kb on - strand at 392.844 kb on - strand at 392.995 kb on + strand, within Rru_A0324 at 392.995 kb on + strand, within Rru_A0324 at 392.995 kb on + strand, within Rru_A0324 at 392.995 kb on + strand, within Rru_A0324 at 392.996 kb on - strand, within Rru_A0324 at 392.996 kb on - strand, within Rru_A0324 at 392.999 kb on + strand, within Rru_A0324 at 392.999 kb on + strand, within Rru_A0324 at 393.000 kb on - strand, within Rru_A0324 at 393.000 kb on - strand, within Rru_A0324 at 393.059 kb on + strand, within Rru_A0324 at 393.059 kb on + strand, within Rru_A0324 at 393.060 kb on - strand, within Rru_A0324
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 390,696 + Rru_A0320 0.76 -0.2 390,696 + Rru_A0320 0.76 +0.9 390,696 + Rru_A0320 0.76 -1.4 390,696 + Rru_A0320 0.76 -0.1 390,790 - Rru_A0320 0.81 +0.5 391,084 - -0.3 391,114 + -0.7 391,115 - +0.2 391,222 + Rru_A0321 0.22 -0.7 391,223 - Rru_A0321 0.23 +0.4 391,223 - Rru_A0321 0.23 +0.5 391,225 + Rru_A0321 0.23 +0.5 391,225 + Rru_A0321 0.23 -0.9 391,226 - Rru_A0321 0.23 -0.1 391,226 - Rru_A0321 0.23 +0.2 391,336 + Rru_A0321 0.44 -2.9 391,337 - Rru_A0321 0.44 +0.9 391,414 + Rru_A0321 0.59 -0.3 391,414 + Rru_A0321 0.59 -0.3 391,415 - Rru_A0321 0.59 +0.4 391,415 - Rru_A0321 0.59 +0.3 391,415 - Rru_A0321 0.59 +3.0 391,459 + Rru_A0321 0.68 -0.3 391,459 + Rru_A0321 0.68 -0.4 391,459 + Rru_A0321 0.68 +1.1 391,460 - Rru_A0321 0.68 +1.9 391,460 - Rru_A0321 0.68 +0.6 391,460 - Rru_A0321 0.68 -1.3 391,460 - Rru_A0321 0.68 -2.3 391,460 - Rru_A0321 0.68 +0.4 391,547 - Rru_A0321 0.85 -1.3 391,547 - Rru_A0321 0.85 -0.1 391,547 - Rru_A0321 0.85 -3.0 391,791 - Rru_A0322 0.33 -0.3 391,791 - Rru_A0322 0.33 +0.0 391,801 + Rru_A0322 0.35 -1.4 391,802 - Rru_A0322 0.36 -0.6 391,802 - Rru_A0322 0.36 -0.2 391,802 - Rru_A0322 0.36 -0.7 391,960 + Rru_A0322 0.70 -1.7 391,961 - Rru_A0322 0.70 +1.3 391,961 - Rru_A0322 0.70 -0.4 392,078 + -0.6 392,078 + -3.0 392,079 - -0.6 392,079 - -0.5 392,079 - +0.3 392,370 - Rru_A0323 0.44 -0.2 392,512 + Rru_A0323 0.70 -0.6 392,513 - Rru_A0323 0.71 -0.7 392,555 - Rru_A0323 0.79 -0.1 392,700 + -0.1 392,700 + +2.7 392,735 + +0.4 392,837 - +0.3 392,844 - +0.6 392,995 + Rru_A0324 0.13 -0.0 392,995 + Rru_A0324 0.13 +1.0 392,995 + Rru_A0324 0.13 +0.7 392,995 + Rru_A0324 0.13 -0.0 392,996 - Rru_A0324 0.13 +0.6 392,996 - Rru_A0324 0.13 +0.2 392,999 + Rru_A0324 0.14 +0.2 392,999 + Rru_A0324 0.14 +0.3 393,000 - Rru_A0324 0.14 +0.7 393,000 - Rru_A0324 0.14 -2.3 393,059 + Rru_A0324 0.16 +0.5 393,059 + Rru_A0324 0.16 +0.2 393,060 - Rru_A0324 0.16 -0.0
Or see this region's nucleotide sequence