Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0149 and Rru_A0150 are separated by 77 nucleotides Rru_A0150 and Rru_A0151 are separated by 61 nucleotides Rru_A0151 and Rru_A0152 overlap by 4 nucleotides
Rru_A0149: Rru_A0149 - PfkB (NCBI), at 173,693 to 174,916
_A0149
Rru_A0150: Rru_A0150 - hypothetical protein (NCBI), at 174,994 to 175,677
_A0150
Rru_A0151: Rru_A0151 - hypothetical protein (NCBI), at 175,739 to 176,482
_A0151
Rru_A0152: Rru_A0152 - hypothetical protein (NCBI), at 176,479 to 176,895
_A0152
Position (kb) 174
175
176 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 174.211 kb on - strand, within Rru_A0149 at 174.222 kb on + strand, within Rru_A0149 at 174.222 kb on + strand, within Rru_A0149 at 174.222 kb on + strand, within Rru_A0149 at 174.223 kb on - strand, within Rru_A0149 at 174.223 kb on - strand, within Rru_A0149 at 174.223 kb on - strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.294 kb on + strand, within Rru_A0149 at 174.295 kb on - strand, within Rru_A0149 at 174.295 kb on - strand, within Rru_A0149 at 174.493 kb on + strand, within Rru_A0149 at 174.493 kb on + strand, within Rru_A0149 at 174.494 kb on - strand, within Rru_A0149 at 174.544 kb on - strand, within Rru_A0149 at 174.695 kb on + strand, within Rru_A0149 at 174.695 kb on + strand, within Rru_A0149 at 174.695 kb on + strand, within Rru_A0149 at 174.696 kb on - strand, within Rru_A0149 at 174.696 kb on - strand, within Rru_A0149 at 174.696 kb on - strand, within Rru_A0149 at 174.696 kb on - strand, within Rru_A0149 at 174.787 kb on + strand, within Rru_A0149 at 174.850 kb on + strand at 174.850 kb on + strand at 174.850 kb on + strand at 174.852 kb on + strand at 174.852 kb on + strand at 174.852 kb on + strand at 174.852 kb on + strand at 174.853 kb on - strand at 174.853 kb on - strand at 174.853 kb on - strand at 175.087 kb on + strand, within Rru_A0150 at 175.371 kb on + strand, within Rru_A0150 at 175.371 kb on + strand, within Rru_A0150 at 175.372 kb on - strand, within Rru_A0150 at 175.374 kb on + strand, within Rru_A0150 at 175.375 kb on - strand, within Rru_A0150 at 175.375 kb on - strand, within Rru_A0150 at 175.393 kb on + strand, within Rru_A0150 at 175.394 kb on - strand, within Rru_A0150 at 175.430 kb on - strand, within Rru_A0150 at 175.444 kb on + strand, within Rru_A0150 at 175.445 kb on - strand, within Rru_A0150 at 175.676 kb on - strand at 175.690 kb on + strand at 175.690 kb on + strand at 175.691 kb on - strand at 176.026 kb on + strand, within Rru_A0151 at 176.027 kb on - strand, within Rru_A0151 at 176.027 kb on - strand, within Rru_A0151
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 174,211 - Rru_A0149 0.42 +0.5 174,222 + Rru_A0149 0.43 +0.4 174,222 + Rru_A0149 0.43 -0.3 174,222 + Rru_A0149 0.43 +0.2 174,223 - Rru_A0149 0.43 -3.5 174,223 - Rru_A0149 0.43 +1.6 174,223 - Rru_A0149 0.43 -0.3 174,294 + Rru_A0149 0.49 -0.2 174,294 + Rru_A0149 0.49 +0.3 174,294 + Rru_A0149 0.49 -0.6 174,294 + Rru_A0149 0.49 +0.7 174,294 + Rru_A0149 0.49 +0.5 174,294 + Rru_A0149 0.49 -0.1 174,294 + Rru_A0149 0.49 +0.1 174,295 - Rru_A0149 0.49 +1.4 174,295 - Rru_A0149 0.49 -0.0 174,493 + Rru_A0149 0.65 +1.1 174,493 + Rru_A0149 0.65 -0.3 174,494 - Rru_A0149 0.65 -0.5 174,544 - Rru_A0149 0.70 -0.7 174,695 + Rru_A0149 0.82 +0.6 174,695 + Rru_A0149 0.82 +0.4 174,695 + Rru_A0149 0.82 -0.3 174,696 - Rru_A0149 0.82 +0.0 174,696 - Rru_A0149 0.82 -0.2 174,696 - Rru_A0149 0.82 -0.4 174,696 - Rru_A0149 0.82 +0.4 174,787 + Rru_A0149 0.89 -0.7 174,850 + -0.1 174,850 + -0.2 174,850 + +0.0 174,852 + +1.0 174,852 + +0.1 174,852 + +1.4 174,852 + +0.4 174,853 - -0.3 174,853 - +1.7 174,853 - -1.0 175,087 + Rru_A0150 0.14 -1.4 175,371 + Rru_A0150 0.55 -1.6 175,371 + Rru_A0150 0.55 -0.1 175,372 - Rru_A0150 0.55 -1.9 175,374 + Rru_A0150 0.56 -1.8 175,375 - Rru_A0150 0.56 -0.1 175,375 - Rru_A0150 0.56 -0.5 175,393 + Rru_A0150 0.58 -0.2 175,394 - Rru_A0150 0.58 -1.7 175,430 - Rru_A0150 0.64 -0.8 175,444 + Rru_A0150 0.66 -0.3 175,445 - Rru_A0150 0.66 -1.9 175,676 - +0.0 175,690 + +0.3 175,690 + +0.3 175,691 - +0.6 176,026 + Rru_A0151 0.39 +0.5 176,027 - Rru_A0151 0.39 -0.9 176,027 - Rru_A0151 0.39 -2.4
Or see this region's nucleotide sequence