Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0069 and Rru_A0070 are separated by 363 nucleotides Rru_A0070 and Rru_A0071 are separated by 327 nucleotides Rru_A0071 and Rru_A0072 are separated by 101 nucleotides
Rru_A0069: Rru_A0069 - ABC transporter component (NCBI), at 79,942 to 80,952
_A0069
Rru_A0070: Rru_A0070 - Protein of unknown function DUF6, transmembrane (NCBI), at 81,316 to 82,311
_A0070
Rru_A0071: Rru_A0071 - hypothetical protein (NCBI), at 82,639 to 82,902
_A0071
Rru_A0072: Rru_A0072 - chaperone protein Hsp90 (NCBI), at 83,004 to 84,884
_A0072
Position (kb) 81
82
83 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 80.351 kb on + strand, within Rru_A0069 at 80.352 kb on - strand, within Rru_A0069 at 80.352 kb on - strand, within Rru_A0069 at 80.352 kb on - strand, within Rru_A0069 at 80.352 kb on - strand, within Rru_A0069 at 80.486 kb on + strand, within Rru_A0069 at 80.487 kb on - strand, within Rru_A0069 at 80.561 kb on + strand, within Rru_A0069 at 80.561 kb on + strand, within Rru_A0069 at 80.561 kb on + strand, within Rru_A0069 at 80.561 kb on + strand, within Rru_A0069 at 80.561 kb on + strand, within Rru_A0069 at 80.562 kb on - strand, within Rru_A0069 at 80.562 kb on - strand, within Rru_A0069 at 80.562 kb on - strand, within Rru_A0069 at 80.562 kb on - strand, within Rru_A0069 at 80.562 kb on - strand, within Rru_A0069 at 80.562 kb on - strand, within Rru_A0069 at 80.837 kb on + strand, within Rru_A0069 at 81.004 kb on + strand at 81.005 kb on - strand at 81.032 kb on - strand at 81.066 kb on + strand at 81.214 kb on + strand at 81.214 kb on + strand at 81.218 kb on + strand at 81.218 kb on + strand at 81.218 kb on + strand at 81.219 kb on - strand at 81.219 kb on - strand at 81.219 kb on - strand at 81.260 kb on + strand at 81.261 kb on - strand at 81.275 kb on + strand at 81.275 kb on + strand at 81.276 kb on - strand at 81.276 kb on - strand at 81.276 kb on - strand at 81.317 kb on + strand at 81.380 kb on + strand at 81.380 kb on + strand at 81.381 kb on - strand at 81.381 kb on - strand at 81.381 kb on - strand at 81.381 kb on - strand at 81.381 kb on - strand at 81.479 kb on + strand, within Rru_A0070 at 81.479 kb on + strand, within Rru_A0070 at 81.479 kb on + strand, within Rru_A0070 at 81.480 kb on - strand, within Rru_A0070 at 81.480 kb on - strand, within Rru_A0070 at 81.480 kb on - strand, within Rru_A0070 at 81.480 kb on - strand, within Rru_A0070 at 81.486 kb on - strand, within Rru_A0070 at 81.486 kb on - strand, within Rru_A0070 at 81.486 kb on - strand, within Rru_A0070 at 81.729 kb on - strand, within Rru_A0070 at 81.729 kb on - strand, within Rru_A0070 at 81.729 kb on - strand, within Rru_A0070 at 81.761 kb on + strand, within Rru_A0070 at 81.762 kb on - strand, within Rru_A0070 at 81.877 kb on + strand, within Rru_A0070 at 81.877 kb on + strand, within Rru_A0070 at 81.877 kb on + strand, within Rru_A0070 at 81.877 kb on + strand, within Rru_A0070 at 82.135 kb on - strand, within Rru_A0070 at 82.165 kb on + strand, within Rru_A0070 at 82.165 kb on + strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.166 kb on - strand, within Rru_A0070 at 82.326 kb on - strand at 82.329 kb on - strand at 82.329 kb on - strand at 82.386 kb on + strand at 82.386 kb on + strand at 82.387 kb on - strand at 82.387 kb on - strand at 82.396 kb on - strand at 82.396 kb on - strand at 82.621 kb on - strand at 82.621 kb on - strand at 82.659 kb on + strand at 82.659 kb on + strand at 82.660 kb on - strand at 82.660 kb on - strand at 82.734 kb on + strand, within Rru_A0071 at 82.734 kb on + strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.735 kb on - strand, within Rru_A0071 at 82.753 kb on - strand, within Rru_A0071 at 82.765 kb on + strand, within Rru_A0071 at 82.765 kb on + strand, within Rru_A0071 at 82.765 kb on + strand, within Rru_A0071 at 83.005 kb on + strand at 83.005 kb on + strand at 83.006 kb on - strand at 83.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 80,351 + Rru_A0069 0.40 +1.6 80,352 - Rru_A0069 0.41 +0.7 80,352 - Rru_A0069 0.41 +0.3 80,352 - Rru_A0069 0.41 -1.7 80,352 - Rru_A0069 0.41 -0.2 80,486 + Rru_A0069 0.54 +0.2 80,487 - Rru_A0069 0.54 -3.1 80,561 + Rru_A0069 0.61 -2.4 80,561 + Rru_A0069 0.61 -0.0 80,561 + Rru_A0069 0.61 -0.9 80,561 + Rru_A0069 0.61 -1.3 80,561 + Rru_A0069 0.61 -0.0 80,562 - Rru_A0069 0.61 -3.9 80,562 - Rru_A0069 0.61 +0.5 80,562 - Rru_A0069 0.61 +1.0 80,562 - Rru_A0069 0.61 +0.4 80,562 - Rru_A0069 0.61 +0.5 80,562 - Rru_A0069 0.61 +0.1 80,837 + Rru_A0069 0.89 +0.3 81,004 + +0.4 81,005 - -0.1 81,032 - -0.6 81,066 + +0.4 81,214 + +0.1 81,214 + +1.6 81,218 + -1.6 81,218 + -0.4 81,218 + +1.2 81,219 - -0.9 81,219 - -0.2 81,219 - -0.4 81,260 + -2.5 81,261 - -4.1 81,275 + +0.6 81,275 + -1.3 81,276 - -1.4 81,276 - +1.1 81,276 - -0.4 81,317 + +1.0 81,380 + -0.7 81,380 + +1.0 81,381 - -1.4 81,381 - +0.3 81,381 - -0.5 81,381 - +0.4 81,381 - -3.2 81,479 + Rru_A0070 0.16 +0.4 81,479 + Rru_A0070 0.16 +0.9 81,479 + Rru_A0070 0.16 +0.7 81,480 - Rru_A0070 0.16 +0.7 81,480 - Rru_A0070 0.16 -0.4 81,480 - Rru_A0070 0.16 +0.4 81,480 - Rru_A0070 0.16 +0.2 81,486 - Rru_A0070 0.17 -0.1 81,486 - Rru_A0070 0.17 +0.4 81,486 - Rru_A0070 0.17 -0.8 81,729 - Rru_A0070 0.41 +0.5 81,729 - Rru_A0070 0.41 +0.6 81,729 - Rru_A0070 0.41 -0.0 81,761 + Rru_A0070 0.45 +1.3 81,762 - Rru_A0070 0.45 +0.7 81,877 + Rru_A0070 0.56 -0.1 81,877 + Rru_A0070 0.56 +1.0 81,877 + Rru_A0070 0.56 -0.0 81,877 + Rru_A0070 0.56 -0.1 82,135 - Rru_A0070 0.82 -0.2 82,165 + Rru_A0070 0.85 -0.0 82,165 + Rru_A0070 0.85 -0.2 82,166 - Rru_A0070 0.85 -0.3 82,166 - Rru_A0070 0.85 -2.6 82,166 - Rru_A0070 0.85 +0.4 82,166 - Rru_A0070 0.85 -0.3 82,326 - +2.1 82,329 - +0.4 82,329 - -0.3 82,386 + +0.1 82,386 + -0.1 82,387 - +0.5 82,387 - +1.2 82,396 - +0.2 82,396 - -0.0 82,621 - -0.1 82,621 - +0.4 82,659 + +0.4 82,659 + -0.5 82,660 - +0.5 82,660 - +0.4 82,734 + Rru_A0071 0.36 +0.3 82,734 + Rru_A0071 0.36 +0.5 82,735 - Rru_A0071 0.36 +0.6 82,735 - Rru_A0071 0.36 +0.3 82,735 - Rru_A0071 0.36 -0.8 82,735 - Rru_A0071 0.36 +0.2 82,753 - Rru_A0071 0.43 +0.7 82,765 + Rru_A0071 0.48 +0.5 82,765 + Rru_A0071 0.48 -0.5 82,765 + Rru_A0071 0.48 -0.0 83,005 + +0.6 83,005 + -1.4 83,006 - -0.7 83,006 - -2.5
Or see this region's nucleotide sequence