Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt masA and hisIE are separated by 29 nucleotides hisIE and Synpcc7942_1996 are separated by 55 nucleotides Synpcc7942_1996 and hliA are separated by 184 nucleotides hliA and Synpcc7942_1998 are separated by 185 nucleotides
Synpcc7942_1994: masA - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, at 2,064,180 to 2,064,896
masA
Synpcc7942_1995: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein, at 2,064,926 to 2,065,561
hisIE
Synpcc7942_1996: Synpcc7942_1996 - hypothetical protein, at 2,065,617 to 2,065,967
_1996
Synpcc7942_1997: hliA - high light-inducible protein, at 2,066,152 to 2,066,370
hliA
Synpcc7942_1998: Synpcc7942_1998 - GAF, at 2,066,556 to 2,067,143
_1998
Position (kb)
2065
2066 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2064.621 kb on - strand, within masA at 2064.655 kb on + strand at 2064.655 kb on + strand, within masA at 2064.658 kb on - strand, within masA at 2064.663 kb on - strand, within masA at 2064.670 kb on - strand, within masA at 2064.691 kb on + strand, within masA at 2064.742 kb on - strand at 2064.770 kb on + strand, within masA at 2064.791 kb on - strand, within masA at 2064.810 kb on + strand, within masA at 2064.826 kb on + strand at 2064.826 kb on + strand at 2064.826 kb on + strand at 2064.845 kb on - strand at 2064.871 kb on - strand at 2064.878 kb on - strand at 2064.918 kb on + strand at 2065.596 kb on - strand at 2065.597 kb on - strand at 2065.625 kb on + strand at 2065.625 kb on + strand at 2065.635 kb on - strand at 2065.679 kb on - strand, within Synpcc7942_1996 at 2065.686 kb on - strand, within Synpcc7942_1996 at 2065.704 kb on - strand, within Synpcc7942_1996 at 2065.717 kb on + strand, within Synpcc7942_1996 at 2065.754 kb on + strand, within Synpcc7942_1996 at 2065.756 kb on + strand, within Synpcc7942_1996 at 2065.763 kb on - strand, within Synpcc7942_1996 at 2065.789 kb on + strand, within Synpcc7942_1996 at 2065.831 kb on - strand, within Synpcc7942_1996 at 2065.831 kb on - strand, within Synpcc7942_1996 at 2065.844 kb on - strand, within Synpcc7942_1996 at 2065.893 kb on + strand, within Synpcc7942_1996 at 2065.902 kb on - strand, within Synpcc7942_1996 at 2065.941 kb on - strand at 2065.971 kb on + strand at 2065.981 kb on + strand at 2066.096 kb on + strand at 2066.113 kb on + strand at 2066.133 kb on + strand at 2066.133 kb on + strand at 2066.141 kb on - strand at 2066.149 kb on + strand at 2066.212 kb on + strand, within hliA at 2066.245 kb on + strand, within hliA at 2066.463 kb on + strand at 2066.532 kb on - strand at 2066.539 kb on - strand at 2066.539 kb on - strand at 2066.552 kb on + strand at 2066.552 kb on + strand at 2066.594 kb on + strand at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.630 kb on + strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.635 kb on + strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.643 kb on - strand, within Synpcc7942_1998 at 2066.657 kb on + strand, within Synpcc7942_1998 at 2066.681 kb on + strand, within Synpcc7942_1998 at 2066.689 kb on + strand at 2066.712 kb on - strand, within Synpcc7942_1998 at 2066.723 kb on - strand, within Synpcc7942_1998 at 2066.739 kb on - strand, within Synpcc7942_1998 at 2066.744 kb on - strand, within Synpcc7942_1998 at 2066.749 kb on - strand, within Synpcc7942_1998 at 2066.762 kb on - strand, within Synpcc7942_1998 at 2066.789 kb on + strand, within Synpcc7942_1998 at 2066.797 kb on - strand, within Synpcc7942_1998 at 2066.810 kb on + strand, within Synpcc7942_1998 at 2066.813 kb on - strand, within Synpcc7942_1998 at 2066.830 kb on + strand, within Synpcc7942_1998 at 2066.836 kb on - strand, within Synpcc7942_1998 at 2066.850 kb on + strand, within Synpcc7942_1998 at 2066.875 kb on + strand, within Synpcc7942_1998 at 2066.876 kb on + strand, within Synpcc7942_1998 at 2066.881 kb on + strand, within Synpcc7942_1998 at 2066.889 kb on - strand, within Synpcc7942_1998 at 2066.892 kb on + strand, within Synpcc7942_1998 at 2066.901 kb on + strand, within Synpcc7942_1998 at 2066.929 kb on - strand, within Synpcc7942_1998
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 2,064,621 - masA Synpcc7942_1994 0.62 +1.8 2,064,655 + +1.2 2,064,655 + masA Synpcc7942_1994 0.66 +1.4 2,064,658 - masA Synpcc7942_1994 0.67 -1.1 2,064,663 - masA Synpcc7942_1994 0.67 +0.9 2,064,670 - masA Synpcc7942_1994 0.68 -0.1 2,064,691 + masA Synpcc7942_1994 0.71 +0.6 2,064,742 - +1.5 2,064,770 + masA Synpcc7942_1994 0.82 +0.5 2,064,791 - masA Synpcc7942_1994 0.85 +2.1 2,064,810 + masA Synpcc7942_1994 0.88 +1.3 2,064,826 + -0.7 2,064,826 + -1.1 2,064,826 + +0.9 2,064,845 - +2.4 2,064,871 - +0.6 2,064,878 - +0.5 2,064,918 + -1.1 2,065,596 - +2.2 2,065,597 - +0.9 2,065,625 + +4.8 2,065,625 + -2.1 2,065,635 - -1.1 2,065,679 - Synpcc7942_1996 0.18 -0.4 2,065,686 - Synpcc7942_1996 0.20 -0.5 2,065,704 - Synpcc7942_1996 0.25 -1.5 2,065,717 + Synpcc7942_1996 0.28 -0.5 2,065,754 + Synpcc7942_1996 0.39 -0.4 2,065,756 + Synpcc7942_1996 0.40 -1.1 2,065,763 - Synpcc7942_1996 0.42 -0.5 2,065,789 + Synpcc7942_1996 0.49 -3.1 2,065,831 - Synpcc7942_1996 0.61 -1.1 2,065,831 - Synpcc7942_1996 0.61 -1.1 2,065,844 - Synpcc7942_1996 0.65 -2.1 2,065,893 + Synpcc7942_1996 0.79 -2.7 2,065,902 - Synpcc7942_1996 0.81 -0.1 2,065,941 - -1.3 2,065,971 + +0.2 2,065,981 + -0.1 2,066,096 + -1.1 2,066,113 + +1.4 2,066,133 + +1.7 2,066,133 + -2.1 2,066,141 - +0.2 2,066,149 + -0.2 2,066,212 + hliA Synpcc7942_1997 0.27 +2.1 2,066,245 + hliA Synpcc7942_1997 0.42 +0.5 2,066,463 + +1.8 2,066,532 - -1.1 2,066,539 - +2.6 2,066,539 - +0.8 2,066,552 + +2.7 2,066,552 + +0.3 2,066,594 + -0.5 2,066,625 + Synpcc7942_1998 0.12 -2.1 2,066,625 + Synpcc7942_1998 0.12 -3.4 2,066,625 + Synpcc7942_1998 0.12 +1.6 2,066,630 + Synpcc7942_1998 0.13 +1.9 2,066,633 - Synpcc7942_1998 0.13 -1.1 2,066,633 - Synpcc7942_1998 0.13 -0.1 2,066,635 + Synpcc7942_1998 0.13 +3.8 2,066,641 - Synpcc7942_1998 0.14 +0.9 2,066,641 - Synpcc7942_1998 0.14 -2.1 2,066,643 - Synpcc7942_1998 0.15 -1.7 2,066,657 + Synpcc7942_1998 0.17 -1.1 2,066,681 + Synpcc7942_1998 0.21 +0.9 2,066,689 + -1.1 2,066,712 - Synpcc7942_1998 0.27 -2.1 2,066,723 - Synpcc7942_1998 0.28 +1.4 2,066,739 - Synpcc7942_1998 0.31 +1.0 2,066,744 - Synpcc7942_1998 0.32 +0.9 2,066,749 - Synpcc7942_1998 0.33 +0.5 2,066,762 - Synpcc7942_1998 0.35 +1.9 2,066,789 + Synpcc7942_1998 0.40 -0.4 2,066,797 - Synpcc7942_1998 0.41 -1.1 2,066,810 + Synpcc7942_1998 0.43 -0.1 2,066,813 - Synpcc7942_1998 0.44 +0.9 2,066,830 + Synpcc7942_1998 0.47 +1.8 2,066,836 - Synpcc7942_1998 0.48 +1.9 2,066,850 + Synpcc7942_1998 0.50 +1.7 2,066,875 + Synpcc7942_1998 0.54 -2.7 2,066,876 + Synpcc7942_1998 0.54 +2.2 2,066,881 + Synpcc7942_1998 0.55 +1.5 2,066,889 - Synpcc7942_1998 0.57 -2.7 2,066,892 + Synpcc7942_1998 0.57 +3.0 2,066,901 + Synpcc7942_1998 0.59 +1.9 2,066,929 - Synpcc7942_1998 0.63 -1.5
Or see this region's nucleotide sequence