Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28540 and RR42_RS28545 are separated by 60 nucleotides RR42_RS28545 and RR42_RS28550 are separated by 10 nucleotides RR42_RS28550 and RR42_RS28555 overlap by 4 nucleotides
RR42_RS28540: RR42_RS28540 - glutamate--pyruvate aminotransferase, at 1,757,844 to 1,759,100
_RS28540
RR42_RS28545: RR42_RS28545 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) <small>(from data)</small>, at 1,759,161 to 1,760,315
_RS28545
RR42_RS28550: RR42_RS28550 - enoyl-CoA hydratase, at 1,760,326 to 1,761,096
_RS28550
RR42_RS28555: RR42_RS28555 - 3-hydroxyisobutyrate dehydrogenase, at 1,761,093 to 1,761,992
_RS28555
Position (kb) 1759
1760
1761 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1758.238 kb on - strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.252 kb on + strand, within RR42_RS28540 at 1758.253 kb on - strand, within RR42_RS28540 at 1758.348 kb on + strand, within RR42_RS28540 at 1758.349 kb on - strand, within RR42_RS28540 at 1758.349 kb on - strand, within RR42_RS28540 at 1758.456 kb on - strand, within RR42_RS28540 at 1758.456 kb on - strand, within RR42_RS28540 at 1758.657 kb on + strand, within RR42_RS28540 at 1758.666 kb on + strand, within RR42_RS28540 at 1758.667 kb on - strand, within RR42_RS28540 at 1758.750 kb on + strand, within RR42_RS28540 at 1758.750 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.829 kb on - strand, within RR42_RS28540 at 1758.829 kb on - strand, within RR42_RS28540 at 1758.831 kb on - strand, within RR42_RS28540 at 1758.942 kb on + strand, within RR42_RS28540 at 1758.943 kb on - strand, within RR42_RS28540 at 1758.954 kb on + strand, within RR42_RS28540 at 1759.112 kb on + strand at 1759.112 kb on + strand at 1759.113 kb on - strand at 1759.460 kb on + strand, within RR42_RS28545 at 1759.780 kb on + strand, within RR42_RS28545 at 1759.780 kb on + strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.970 kb on - strand, within RR42_RS28545 at 1760.050 kb on + strand, within RR42_RS28545 at 1760.051 kb on - strand, within RR42_RS28545 at 1760.327 kb on + strand at 1760.328 kb on - strand at 1760.487 kb on - strand, within RR42_RS28550 at 1760.793 kb on - strand, within RR42_RS28550 at 1760.992 kb on + strand, within RR42_RS28550 at 1761.121 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 1,758,238 - RR42_RS28540 0.31 -0.6 1,758,248 + RR42_RS28540 0.32 +1.0 1,758,248 + RR42_RS28540 0.32 +0.1 1,758,248 + RR42_RS28540 0.32 +0.1 1,758,248 + RR42_RS28540 0.32 -0.3 1,758,248 + RR42_RS28540 0.32 -0.9 1,758,249 - RR42_RS28540 0.32 -1.7 1,758,249 - RR42_RS28540 0.32 +1.2 1,758,249 - RR42_RS28540 0.32 +0.1 1,758,252 + RR42_RS28540 0.32 -0.6 1,758,253 - RR42_RS28540 0.33 -0.2 1,758,348 + RR42_RS28540 0.40 -0.4 1,758,349 - RR42_RS28540 0.40 -0.1 1,758,349 - RR42_RS28540 0.40 +0.4 1,758,456 - RR42_RS28540 0.49 -1.1 1,758,456 - RR42_RS28540 0.49 -0.4 1,758,657 + RR42_RS28540 0.65 +0.8 1,758,666 + RR42_RS28540 0.65 -0.1 1,758,667 - RR42_RS28540 0.65 +0.6 1,758,750 + RR42_RS28540 0.72 +0.2 1,758,750 + RR42_RS28540 0.72 -0.8 1,758,828 + RR42_RS28540 0.78 -0.5 1,758,828 + RR42_RS28540 0.78 +0.6 1,758,828 + RR42_RS28540 0.78 +1.0 1,758,828 + RR42_RS28540 0.78 +0.2 1,758,829 - RR42_RS28540 0.78 -2.5 1,758,829 - RR42_RS28540 0.78 -0.7 1,758,831 - RR42_RS28540 0.79 -1.4 1,758,942 + RR42_RS28540 0.87 +0.2 1,758,943 - RR42_RS28540 0.87 -0.2 1,758,954 + RR42_RS28540 0.88 +0.1 1,759,112 + -0.0 1,759,112 + -1.2 1,759,113 - -0.0 1,759,460 + RR42_RS28545 0.26 -0.6 1,759,780 + RR42_RS28545 0.54 +0.6 1,759,780 + RR42_RS28545 0.54 -1.3 1,759,781 - RR42_RS28545 0.54 -0.9 1,759,781 - RR42_RS28545 0.54 -1.7 1,759,781 - RR42_RS28545 0.54 -3.0 1,759,970 - RR42_RS28545 0.70 -2.7 1,760,050 + RR42_RS28545 0.77 -1.9 1,760,051 - RR42_RS28545 0.77 -2.3 1,760,327 + -1.2 1,760,328 - -0.8 1,760,487 - RR42_RS28550 0.21 -0.8 1,760,793 - RR42_RS28550 0.61 +0.2 1,760,992 + RR42_RS28550 0.86 -1.8 1,761,121 + -0.7
Or see this region's nucleotide sequence