Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02085 and RR42_RS02090 are separated by 138 nucleotides RR42_RS02090 and RR42_RS02095 are separated by 24 nucleotides RR42_RS02095 and RR42_RS02100 are separated by 54 nucleotides
RR42_RS02085: RR42_RS02085 - RNA polymerase subunit sigma-54, at 463,217 to 464,692
_RS02085
RR42_RS02090: RR42_RS02090 - sugar ABC transporter ATP-binding protein, at 464,831 to 465,616
_RS02090
RR42_RS02095: RR42_RS02095 - organic solvent tolerance protein OstA, at 465,641 to 466,285
_RS02095
RR42_RS02100: RR42_RS02100 - hypothetical protein, at 466,340 to 466,966
_RS02100
Position (kb) 464
465
466 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 463.857 kb on + strand, within RR42_RS02085 at 464.188 kb on - strand, within RR42_RS02085 at 464.221 kb on + strand, within RR42_RS02085 at 464.403 kb on + strand, within RR42_RS02085 at 464.404 kb on - strand, within RR42_RS02085 at 464.647 kb on + strand at 464.679 kb on - strand at 464.679 kb on - strand at 464.699 kb on + strand at 464.704 kb on + strand at 464.704 kb on + strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.741 kb on - strand at 464.773 kb on - strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.833 kb on - strand at 464.833 kb on - strand at 466.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 463,857 + RR42_RS02085 0.43 -4.6 464,188 - RR42_RS02085 0.66 -1.3 464,221 + RR42_RS02085 0.68 -2.7 464,403 + RR42_RS02085 0.80 -2.0 464,404 - RR42_RS02085 0.80 -4.1 464,647 + -2.1 464,679 - -2.4 464,679 - -3.7 464,699 + -2.4 464,704 + +1.6 464,704 + +0.6 464,705 - +0.1 464,705 - +0.6 464,705 - +1.0 464,705 - -0.7 464,741 - -2.1 464,773 - -0.3 464,832 + -1.5 464,832 + -0.1 464,832 + +0.8 464,832 + -0.9 464,832 + +2.0 464,832 + +0.0 464,833 - +0.2 464,833 - -0.5 466,389 - -0.9
Or see this region's nucleotide sequence