Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_74 and Pf1N1B4_75 are separated by 16 nucleotides Pf1N1B4_75 and Pf1N1B4_76 are separated by 501 nucleotides Pf1N1B4_76 and Pf1N1B4_77 are separated by 307 nucleotides Pf1N1B4_77 and Pf1N1B4_78 are separated by 143 nucleotides
Pf1N1B4_74 - hypothetical protein, at 78,166 to 78,429
_74
Pf1N1B4_75 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 78,446 to 78,823
_75
Pf1N1B4_76 - ATP binding protein, at 79,325 to 80,692
_76
Pf1N1B4_77 - FIG00956064: hypothetical protein, at 81,000 to 81,317
_77
Pf1N1B4_78 - FIG00956937: hypothetical protein, at 81,461 to 81,994
_78
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.685 kb on + strand, within Pf1N1B4_75 at 78.685 kb on + strand, within Pf1N1B4_75 at 78.686 kb on - strand, within Pf1N1B4_75 at 78.813 kb on - strand at 78.844 kb on + strand at 78.888 kb on + strand at 79.002 kb on + strand at 79.003 kb on - strand at 79.181 kb on + strand at 79.181 kb on + strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.200 kb on + strand at 79.201 kb on - strand at 79.236 kb on - strand at 79.276 kb on + strand at 79.276 kb on + strand at 79.277 kb on - strand at 79.277 kb on - strand at 79.324 kb on - strand at 79.587 kb on + strand at 79.785 kb on - strand, within Pf1N1B4_76 at 80.281 kb on - strand, within Pf1N1B4_76 at 80.281 kb on - strand, within Pf1N1B4_76 at 80.344 kb on - strand, within Pf1N1B4_76 at 80.374 kb on - strand, within Pf1N1B4_76 at 80.449 kb on - strand, within Pf1N1B4_76 at 80.503 kb on - strand, within Pf1N1B4_76 at 80.659 kb on - strand at 80.705 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.824 kb on + strand at 80.824 kb on + strand at 80.824 kb on + strand at 80.825 kb on - strand at 80.825 kb on - strand at 80.877 kb on - strand at 80.877 kb on - strand at 80.911 kb on + strand at 80.927 kb on - strand at 81.030 kb on + strand at 81.030 kb on + strand at 81.031 kb on - strand at 81.071 kb on + strand, within Pf1N1B4_77 at 81.071 kb on + strand, within Pf1N1B4_77 at 81.071 kb on + strand, within Pf1N1B4_77 at 81.137 kb on + strand, within Pf1N1B4_77 at 81.162 kb on + strand, within Pf1N1B4_77 at 81.220 kb on + strand, within Pf1N1B4_77 at 81.289 kb on + strand at 81.290 kb on - strand at 81.290 kb on - strand at 81.315 kb on + strand at 81.316 kb on - strand at 81.334 kb on + strand at 81.335 kb on - strand at 81.335 kb on - strand at 81.403 kb on - strand at 81.403 kb on - strand at 81.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 78,419 + -0.8 78,419 + +2.1 78,419 + +0.8 78,419 + -0.7 78,420 - +0.7 78,420 - +0.4 78,420 - -0.2 78,420 - +0.8 78,420 - -0.7 78,685 + Pf1N1B4_75 0.63 -0.5 78,685 + Pf1N1B4_75 0.63 +0.4 78,686 - Pf1N1B4_75 0.63 +0.1 78,813 - +0.8 78,844 + -0.2 78,888 + -0.7 79,002 + -1.7 79,003 - -0.7 79,181 + +1.2 79,181 + +0.3 79,182 - +1.1 79,182 - -0.4 79,182 - -0.2 79,182 - +1.4 79,200 + +0.6 79,201 - +1.8 79,236 - +0.3 79,276 + -0.2 79,276 + -0.5 79,277 - -0.2 79,277 - -0.5 79,324 - +0.4 79,587 + -0.5 79,785 - Pf1N1B4_76 0.34 -1.2 80,281 - Pf1N1B4_76 0.70 +1.3 80,281 - Pf1N1B4_76 0.70 -1.3 80,344 - Pf1N1B4_76 0.74 -1.5 80,374 - Pf1N1B4_76 0.77 +0.8 80,449 - Pf1N1B4_76 0.82 +0.0 80,503 - Pf1N1B4_76 0.86 -2.1 80,659 - -0.0 80,705 + -2.0 80,752 + -0.6 80,752 + +1.4 80,752 + -0.5 80,752 + +0.0 80,753 - +0.6 80,753 - -2.0 80,753 - -0.3 80,753 - -0.2 80,824 + -0.6 80,824 + -1.2 80,824 + +0.4 80,825 - +0.8 80,825 - -0.7 80,877 - +0.7 80,877 - -0.2 80,911 + +0.1 80,927 - -1.0 81,030 + +0.9 81,030 + +0.1 81,031 - +1.8 81,071 + Pf1N1B4_77 0.22 +0.0 81,071 + Pf1N1B4_77 0.22 +1.6 81,071 + Pf1N1B4_77 0.22 +0.3 81,137 + Pf1N1B4_77 0.43 +1.2 81,162 + Pf1N1B4_77 0.51 +1.7 81,220 + Pf1N1B4_77 0.69 +0.2 81,289 + -0.5 81,290 - -0.2 81,290 - +1.6 81,315 + -0.2 81,316 - -1.2 81,334 + +2.1 81,335 - +0.3 81,335 - -0.6 81,403 - +2.7 81,403 - +1.5 81,426 + +0.6
Or see this region's nucleotide sequence