Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5777 and Pf1N1B4_5778 are separated by 31 nucleotides Pf1N1B4_5778 and Pf1N1B4_5779 overlap by 4 nucleotides Pf1N1B4_5779 and Pf1N1B4_5780 overlap by 4 nucleotides
Pf1N1B4_5777 - Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL, at 214,238 to 214,765
_5777
Pf1N1B4_5778 - Nitrous oxide reductase maturation transmembrane protein NosY, at 214,797 to 215,627
_5778
Pf1N1B4_5779 - Nitrous oxide reductase maturation protein NosF (ATPase), at 215,624 to 216,547
_5779
Pf1N1B4_5780 - Nitrous oxide reductase maturation protein NosD, at 216,544 to 217,836
_5780
Position (kb) 215
216
217 Strain fitness (log2 ratio) -2
-1
0
1 at 214.718 kb on + strand at 214.719 kb on - strand at 214.760 kb on - strand at 214.760 kb on - strand at 214.986 kb on + strand, within Pf1N1B4_5778 at 215.143 kb on + strand, within Pf1N1B4_5778 at 215.668 kb on - strand at 215.668 kb on - strand at 215.676 kb on + strand at 215.735 kb on + strand, within Pf1N1B4_5779 at 215.736 kb on - strand, within Pf1N1B4_5779 at 215.987 kb on + strand, within Pf1N1B4_5779 at 215.987 kb on + strand at 215.988 kb on - strand, within Pf1N1B4_5779 at 215.988 kb on - strand at 216.291 kb on + strand, within Pf1N1B4_5779 at 216.505 kb on - strand at 216.510 kb on + strand at 216.738 kb on - strand at 216.842 kb on + strand at 216.843 kb on - strand, within Pf1N1B4_5780 at 216.975 kb on - strand, within Pf1N1B4_5780 at 217.070 kb on + strand, within Pf1N1B4_5780 at 217.079 kb on + strand, within Pf1N1B4_5780 at 217.080 kb on - strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.221 kb on - strand at 217.289 kb on + strand, within Pf1N1B4_5780 at 217.289 kb on + strand, within Pf1N1B4_5780 at 217.349 kb on + strand, within Pf1N1B4_5780
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 214,718 + +0.6 214,719 - +1.6 214,760 - -0.7 214,760 - -2.2 214,986 + Pf1N1B4_5778 0.23 -0.4 215,143 + Pf1N1B4_5778 0.42 +1.1 215,668 - -2.6 215,668 - -0.3 215,676 + +0.7 215,735 + Pf1N1B4_5779 0.12 -1.2 215,736 - Pf1N1B4_5779 0.12 -0.4 215,987 + Pf1N1B4_5779 0.39 -1.6 215,987 + +1.6 215,988 - Pf1N1B4_5779 0.39 -0.6 215,988 - -1.4 216,291 + Pf1N1B4_5779 0.72 -0.0 216,505 - -1.7 216,510 + -0.4 216,738 - -1.0 216,842 + -0.8 216,843 - Pf1N1B4_5780 0.23 -0.4 216,975 - Pf1N1B4_5780 0.33 +1.2 217,070 + Pf1N1B4_5780 0.41 +1.1 217,079 + Pf1N1B4_5780 0.41 -0.9 217,080 - Pf1N1B4_5780 0.41 +0.6 217,220 + Pf1N1B4_5780 0.52 -0.7 217,220 + Pf1N1B4_5780 0.52 -0.8 217,220 + Pf1N1B4_5780 0.52 +0.2 217,220 + Pf1N1B4_5780 0.52 -2.0 217,220 + Pf1N1B4_5780 0.52 +0.6 217,220 + Pf1N1B4_5780 0.52 +1.2 217,221 - +1.6 217,289 + Pf1N1B4_5780 0.58 +0.6 217,289 + Pf1N1B4_5780 0.58 -0.7 217,349 + Pf1N1B4_5780 0.62 +0.6
Or see this region's nucleotide sequence