Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5698 and Pf1N1B4_5699 are separated by 139 nucleotides Pf1N1B4_5699 and Pf1N1B4_5700 are separated by 166 nucleotides
Pf1N1B4_5698 - Putative monooxygenase, at 136,882 to 138,258
_5698
Pf1N1B4_5699 - ABC-type uncharacterized transport system, ATPase component, at 138,398 to 139,000
_5699
Pf1N1B4_5700 - Putative tRNA-m1A22 methylase, at 139,167 to 139,841
_5700
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 137.431 kb on + strand, within Pf1N1B4_5698 at 137.432 kb on - strand at 137.432 kb on - strand, within Pf1N1B4_5698 at 137.489 kb on - strand, within Pf1N1B4_5698 at 137.697 kb on - strand, within Pf1N1B4_5698 at 137.804 kb on + strand, within Pf1N1B4_5698 at 137.952 kb on - strand, within Pf1N1B4_5698 at 137.952 kb on - strand, within Pf1N1B4_5698 at 137.967 kb on + strand, within Pf1N1B4_5698 at 137.968 kb on - strand, within Pf1N1B4_5698 at 137.970 kb on - strand, within Pf1N1B4_5698 at 137.970 kb on - strand at 137.972 kb on - strand, within Pf1N1B4_5698 at 137.978 kb on - strand, within Pf1N1B4_5698 at 138.085 kb on + strand, within Pf1N1B4_5698 at 138.235 kb on - strand at 138.351 kb on - strand at 138.385 kb on + strand at 138.467 kb on + strand, within Pf1N1B4_5699 at 138.488 kb on - strand, within Pf1N1B4_5699 at 138.488 kb on - strand, within Pf1N1B4_5699 at 138.488 kb on - strand at 138.717 kb on + strand, within Pf1N1B4_5699 at 138.718 kb on - strand, within Pf1N1B4_5699 at 139.058 kb on + strand at 139.065 kb on - strand at 139.096 kb on - strand at 139.096 kb on - strand at 139.144 kb on + strand at 139.346 kb on - strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.403 kb on - strand, within Pf1N1B4_5700 at 139.406 kb on - strand, within Pf1N1B4_5700 at 139.679 kb on + strand, within Pf1N1B4_5700 at 139.679 kb on + strand, within Pf1N1B4_5700 at 139.812 kb on + strand at 139.812 kb on + strand at 139.812 kb on + strand at 139.813 kb on - strand at 139.813 kb on - strand at 139.876 kb on + strand at 139.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 137,431 + Pf1N1B4_5698 0.40 +0.0 137,432 - -0.4 137,432 - Pf1N1B4_5698 0.40 -0.1 137,489 - Pf1N1B4_5698 0.44 -0.7 137,697 - Pf1N1B4_5698 0.59 +1.0 137,804 + Pf1N1B4_5698 0.67 -0.6 137,952 - Pf1N1B4_5698 0.78 -0.1 137,952 - Pf1N1B4_5698 0.78 -0.7 137,967 + Pf1N1B4_5698 0.79 -1.0 137,968 - Pf1N1B4_5698 0.79 -0.4 137,970 - Pf1N1B4_5698 0.79 +0.6 137,970 - +2.2 137,972 - Pf1N1B4_5698 0.79 -0.4 137,978 - Pf1N1B4_5698 0.80 +0.9 138,085 + Pf1N1B4_5698 0.87 -1.4 138,235 - +0.6 138,351 - +1.2 138,385 + -0.9 138,467 + Pf1N1B4_5699 0.11 -0.7 138,488 - Pf1N1B4_5699 0.15 -2.0 138,488 - Pf1N1B4_5699 0.15 -1.4 138,488 - -1.0 138,717 + Pf1N1B4_5699 0.53 +1.6 138,718 - Pf1N1B4_5699 0.53 -1.0 139,058 + -0.4 139,065 - -1.2 139,096 - -1.0 139,096 - +1.6 139,144 + +1.4 139,346 - Pf1N1B4_5700 0.27 -0.5 139,363 + Pf1N1B4_5700 0.29 +1.0 139,363 + Pf1N1B4_5700 0.29 +1.6 139,363 + Pf1N1B4_5700 0.29 +0.8 139,364 - Pf1N1B4_5700 0.29 +0.3 139,364 - Pf1N1B4_5700 0.29 -0.4 139,364 - Pf1N1B4_5700 0.29 -0.6 139,403 - Pf1N1B4_5700 0.35 +0.6 139,406 - Pf1N1B4_5700 0.35 -0.1 139,679 + Pf1N1B4_5700 0.76 +0.6 139,679 + Pf1N1B4_5700 0.76 -0.2 139,812 + -0.7 139,812 + +0.6 139,812 + -0.7 139,813 - -0.4 139,813 - -1.0 139,876 + -0.2 139,877 - +0.6
Or see this region's nucleotide sequence