Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5586 and Pf1N1B4_5587 overlap by 4 nucleotides Pf1N1B4_5587 and Pf1N1B4_5588 are separated by 10 nucleotides Pf1N1B4_5588 and Pf1N1B4_5589 are separated by 83 nucleotides
Pf1N1B4_5586 - Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-), at 7,744 to 8,790
_5586
Pf1N1B4_5587 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 8,787 to 9,737
_5587
Pf1N1B4_5588 - Transcriptional regulator for ferulate or vanillate catabolism, at 9,748 to 10,461
_5588
Pf1N1B4_5589 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 10,545 to 11,786
_5589
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 9.265 kb on + strand, within Pf1N1B4_5587 at 9.265 kb on + strand, within Pf1N1B4_5587 at 9.265 kb on + strand, within Pf1N1B4_5587 at 9.266 kb on - strand at 9.266 kb on - strand at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.347 kb on - strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.349 kb on - strand at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.547 kb on + strand, within Pf1N1B4_5587 at 9.548 kb on - strand, within Pf1N1B4_5587 at 9.739 kb on - strand at 9.750 kb on - strand at 9.914 kb on + strand, within Pf1N1B4_5588 at 9.915 kb on - strand, within Pf1N1B4_5588 at 10.093 kb on + strand, within Pf1N1B4_5588 at 10.093 kb on + strand, within Pf1N1B4_5588 at 10.094 kb on - strand, within Pf1N1B4_5588 at 10.456 kb on - strand at 10.492 kb on + strand at 10.492 kb on + strand at 10.492 kb on + strand at 10.493 kb on - strand at 10.506 kb on - strand at 10.506 kb on - strand at 11.111 kb on + strand, within Pf1N1B4_5589 at 11.112 kb on - strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.314 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 9,265 + Pf1N1B4_5587 0.50 -2.4 9,265 + Pf1N1B4_5587 0.50 -1.7 9,265 + Pf1N1B4_5587 0.50 +2.1 9,266 - -0.0 9,266 - +1.6 9,346 + Pf1N1B4_5587 0.59 +0.2 9,346 + Pf1N1B4_5587 0.59 +0.3 9,346 + Pf1N1B4_5587 0.59 +0.4 9,346 + Pf1N1B4_5587 0.59 +0.1 9,346 + Pf1N1B4_5587 0.59 -0.4 9,347 - Pf1N1B4_5587 0.59 +0.6 9,348 + Pf1N1B4_5587 0.59 +0.6 9,348 + Pf1N1B4_5587 0.59 -0.4 9,348 + Pf1N1B4_5587 0.59 +0.2 9,348 + Pf1N1B4_5587 0.59 +0.5 9,348 + Pf1N1B4_5587 0.59 +2.2 9,349 - +2.6 9,349 - Pf1N1B4_5587 0.59 -0.3 9,349 - Pf1N1B4_5587 0.59 +0.4 9,349 - Pf1N1B4_5587 0.59 +0.6 9,349 - Pf1N1B4_5587 0.59 +0.1 9,547 + Pf1N1B4_5587 0.80 +1.0 9,548 - Pf1N1B4_5587 0.80 +0.9 9,739 - -0.4 9,750 - +0.6 9,914 + Pf1N1B4_5588 0.23 -0.3 9,915 - Pf1N1B4_5588 0.23 -1.5 10,093 + Pf1N1B4_5588 0.48 -1.8 10,093 + Pf1N1B4_5588 0.48 -1.7 10,094 - Pf1N1B4_5588 0.48 +0.4 10,456 - -1.1 10,492 + +0.1 10,492 + -0.0 10,492 + +1.2 10,493 - -0.2 10,506 - +0.6 10,506 - +0.6 11,111 + Pf1N1B4_5589 0.46 +0.6 11,112 - Pf1N1B4_5589 0.46 -0.8 11,295 + Pf1N1B4_5589 0.60 +1.3 11,295 + Pf1N1B4_5589 0.60 +0.2 11,314 + Pf1N1B4_5589 0.62 +0.6 11,383 + Pf1N1B4_5589 0.67 +0.1 11,383 + Pf1N1B4_5589 0.67 -0.8
Or see this region's nucleotide sequence