Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_555 and Pf1N1B4_556 are separated by 166 nucleotides Pf1N1B4_556 and Pf1N1B4_557 are separated by 83 nucleotides Pf1N1B4_557 and Pf1N1B4_558 are separated by 215 nucleotides
Pf1N1B4_555 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 651,653 to 652,870
_555
Pf1N1B4_556 - Gluconate permease, at 653,037 to 654,389
_556
Pf1N1B4_557 - Gluconokinase (EC 2.7.1.12), at 654,473 to 655,006
_557
Pf1N1B4_558 - Gluconate utilization system Gnt-I transcriptional repressor, at 655,222 to 656,253
_558
Position (kb) 653
654
655 Strain fitness (log2 ratio) -2
-1
0
1
2 at 652.061 kb on + strand, within Pf1N1B4_555 at 652.061 kb on + strand at 652.061 kb on + strand, within Pf1N1B4_555 at 652.062 kb on - strand, within Pf1N1B4_555 at 652.062 kb on - strand, within Pf1N1B4_555 at 652.062 kb on - strand, within Pf1N1B4_555 at 652.259 kb on + strand at 652.388 kb on + strand, within Pf1N1B4_555 at 652.388 kb on + strand, within Pf1N1B4_555 at 652.389 kb on - strand, within Pf1N1B4_555 at 652.466 kb on + strand at 652.466 kb on + strand, within Pf1N1B4_555 at 652.466 kb on + strand, within Pf1N1B4_555 at 652.466 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.664 kb on + strand, within Pf1N1B4_555 at 652.751 kb on + strand at 652.751 kb on + strand at 653.143 kb on + strand at 653.522 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.533 kb on + strand, within Pf1N1B4_556 at 653.534 kb on - strand at 653.534 kb on - strand, within Pf1N1B4_556 at 653.534 kb on - strand, within Pf1N1B4_556 at 653.534 kb on - strand, within Pf1N1B4_556 at 653.534 kb on - strand, within Pf1N1B4_556 at 653.783 kb on + strand, within Pf1N1B4_556 at 653.784 kb on - strand, within Pf1N1B4_556 at 653.784 kb on - strand at 653.917 kb on - strand, within Pf1N1B4_556 at 654.326 kb on + strand at 654.327 kb on - strand at 654.354 kb on + strand at 654.923 kb on - strand, within Pf1N1B4_557 at 655.009 kb on - strand at 655.047 kb on - strand at 655.047 kb on - strand at 655.189 kb on + strand at 655.189 kb on + strand at 655.227 kb on + strand at 655.248 kb on + strand at 655.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 652,061 + Pf1N1B4_555 0.33 -0.0 652,061 + +2.6 652,061 + Pf1N1B4_555 0.33 +1.3 652,062 - Pf1N1B4_555 0.34 +1.0 652,062 - Pf1N1B4_555 0.34 +0.2 652,062 - Pf1N1B4_555 0.34 -0.6 652,259 + -0.0 652,388 + Pf1N1B4_555 0.60 -2.4 652,388 + Pf1N1B4_555 0.60 +0.2 652,389 - Pf1N1B4_555 0.60 -1.6 652,466 + +1.0 652,466 + Pf1N1B4_555 0.67 +0.1 652,466 + Pf1N1B4_555 0.67 -2.4 652,466 + Pf1N1B4_555 0.67 +0.6 652,637 + +1.0 652,637 + Pf1N1B4_555 0.81 +0.4 652,637 + Pf1N1B4_555 0.81 +1.1 652,637 + Pf1N1B4_555 0.81 -0.6 652,637 + Pf1N1B4_555 0.81 +0.2 652,637 + Pf1N1B4_555 0.81 +0.1 652,637 + Pf1N1B4_555 0.81 -0.3 652,637 + Pf1N1B4_555 0.81 -0.6 652,637 + Pf1N1B4_555 0.81 -0.4 652,637 + Pf1N1B4_555 0.81 +0.6 652,637 + Pf1N1B4_555 0.81 -1.0 652,637 + Pf1N1B4_555 0.81 +0.4 652,664 + Pf1N1B4_555 0.83 +0.8 652,751 + +0.6 652,751 + -0.0 653,143 + -1.4 653,522 - Pf1N1B4_556 0.36 -1.0 653,532 - Pf1N1B4_556 0.37 -0.3 653,532 - Pf1N1B4_556 0.37 -2.4 653,532 - Pf1N1B4_556 0.37 -0.8 653,532 - Pf1N1B4_556 0.37 +0.1 653,532 - Pf1N1B4_556 0.37 -1.0 653,532 - Pf1N1B4_556 0.37 -1.5 653,533 + Pf1N1B4_556 0.37 +1.0 653,534 - +2.3 653,534 - Pf1N1B4_556 0.37 -1.2 653,534 - Pf1N1B4_556 0.37 -1.4 653,534 - Pf1N1B4_556 0.37 +1.0 653,534 - Pf1N1B4_556 0.37 +1.0 653,783 + Pf1N1B4_556 0.55 -1.0 653,784 - Pf1N1B4_556 0.55 +1.0 653,784 - -1.6 653,917 - Pf1N1B4_556 0.65 +0.1 654,326 + +0.6 654,327 - -1.0 654,354 + +1.0 654,923 - Pf1N1B4_557 0.84 +1.0 655,009 - +0.2 655,047 - +0.6 655,047 - -2.5 655,189 + -0.4 655,189 + -1.4 655,227 + -2.6 655,248 + +1.2 655,312 - +0.6
Or see this region's nucleotide sequence