Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5529 and Pf1N1B4_5530 are separated by 161 nucleotides Pf1N1B4_5530 and Pf1N1B4_5531 are separated by 79 nucleotides
Pf1N1B4_5529 - hypothetical protein, at 288,321 to 289,550
_5529
Pf1N1B4_5530 - hypothetical protein, at 289,712 to 290,338
_5530
Pf1N1B4_5531 - hypothetical protein, at 290,418 to 290,804
_5531
Position (kb) 289
290
291 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 288.764 kb on + strand, within Pf1N1B4_5529 at 288.765 kb on - strand, within Pf1N1B4_5529 at 288.765 kb on - strand, within Pf1N1B4_5529 at 288.839 kb on + strand, within Pf1N1B4_5529 at 288.839 kb on + strand, within Pf1N1B4_5529 at 288.840 kb on - strand, within Pf1N1B4_5529 at 288.840 kb on - strand, within Pf1N1B4_5529 at 288.840 kb on - strand, within Pf1N1B4_5529 at 288.871 kb on + strand, within Pf1N1B4_5529 at 289.052 kb on - strand, within Pf1N1B4_5529 at 289.180 kb on + strand, within Pf1N1B4_5529 at 289.180 kb on + strand, within Pf1N1B4_5529 at 289.181 kb on - strand, within Pf1N1B4_5529 at 289.181 kb on - strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.366 kb on + strand, within Pf1N1B4_5529 at 289.366 kb on + strand, within Pf1N1B4_5529 at 289.366 kb on + strand, within Pf1N1B4_5529 at 289.367 kb on - strand, within Pf1N1B4_5529 at 289.495 kb on + strand at 289.495 kb on + strand at 289.495 kb on + strand at 289.495 kb on + strand at 289.495 kb on + strand at 289.496 kb on - strand at 289.496 kb on - strand at 289.496 kb on - strand at 289.496 kb on - strand at 289.511 kb on + strand at 289.544 kb on + strand at 289.545 kb on - strand at 289.594 kb on + strand at 289.594 kb on + strand at 289.731 kb on + strand at 289.732 kb on - strand at 289.810 kb on - strand, within Pf1N1B4_5530 at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.992 kb on + strand at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.993 kb on - strand, within Pf1N1B4_5530 at 289.993 kb on - strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.042 kb on - strand at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.059 kb on + strand, within Pf1N1B4_5530 at 290.068 kb on - strand, within Pf1N1B4_5530 at 290.095 kb on + strand, within Pf1N1B4_5530 at 290.105 kb on + strand at 290.106 kb on - strand, within Pf1N1B4_5530 at 290.152 kb on + strand, within Pf1N1B4_5530 at 290.153 kb on - strand, within Pf1N1B4_5530 at 290.153 kb on - strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.233 kb on - strand, within Pf1N1B4_5530 at 290.255 kb on + strand, within Pf1N1B4_5530 at 290.275 kb on + strand, within Pf1N1B4_5530 at 290.275 kb on + strand, within Pf1N1B4_5530 at 290.275 kb on + strand, within Pf1N1B4_5530 at 290.276 kb on - strand at 290.276 kb on - strand at 290.276 kb on - strand at 290.276 kb on - strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.307 kb on - strand at 290.307 kb on - strand at 290.307 kb on - strand at 290.307 kb on - strand at 290.408 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.493 kb on + strand at 290.493 kb on + strand, within Pf1N1B4_5531 at 290.493 kb on + strand, within Pf1N1B4_5531 at 290.493 kb on + strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.497 kb on + strand, within Pf1N1B4_5531 at 290.498 kb on - strand, within Pf1N1B4_5531 at 290.498 kb on - strand, within Pf1N1B4_5531 at 290.506 kb on + strand, within Pf1N1B4_5531 at 290.772 kb on + strand at 290.878 kb on + strand at 290.878 kb on + strand at 290.878 kb on + strand at 290.879 kb on - strand at 290.879 kb on - strand at 290.925 kb on + strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.037 kb on + strand at 291.037 kb on + strand at 291.037 kb on + strand at 291.055 kb on + strand at 291.056 kb on - strand at 291.092 kb on + strand at 291.092 kb on + strand at 291.092 kb on + strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.097 kb on - strand at 291.097 kb on - strand at 291.181 kb on + strand at 291.182 kb on - strand at 291.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 288,764 + Pf1N1B4_5529 0.36 +0.9 288,765 - Pf1N1B4_5529 0.36 +1.0 288,765 - Pf1N1B4_5529 0.36 +0.3 288,839 + Pf1N1B4_5529 0.42 +0.2 288,839 + Pf1N1B4_5529 0.42 -0.5 288,840 - Pf1N1B4_5529 0.42 +0.5 288,840 - Pf1N1B4_5529 0.42 -0.5 288,840 - Pf1N1B4_5529 0.42 -0.5 288,871 + Pf1N1B4_5529 0.45 +0.2 289,052 - Pf1N1B4_5529 0.59 -0.5 289,180 + Pf1N1B4_5529 0.70 -0.0 289,180 + Pf1N1B4_5529 0.70 -0.0 289,181 - Pf1N1B4_5529 0.70 -1.0 289,181 - Pf1N1B4_5529 0.70 -1.2 289,201 + Pf1N1B4_5529 0.72 -2.0 289,201 + Pf1N1B4_5529 0.72 -0.2 289,201 + Pf1N1B4_5529 0.72 -0.9 289,201 + Pf1N1B4_5529 0.72 -1.8 289,202 - Pf1N1B4_5529 0.72 -0.5 289,202 - Pf1N1B4_5529 0.72 -0.3 289,202 - Pf1N1B4_5529 0.72 +0.2 289,202 - Pf1N1B4_5529 0.72 +1.4 289,366 + Pf1N1B4_5529 0.85 -0.2 289,366 + Pf1N1B4_5529 0.85 -0.3 289,366 + Pf1N1B4_5529 0.85 +1.0 289,367 - Pf1N1B4_5529 0.85 -0.3 289,495 + -0.2 289,495 + +0.5 289,495 + +3.3 289,495 + -1.8 289,495 + -0.5 289,496 - +0.2 289,496 - +0.4 289,496 - -2.3 289,496 - -0.6 289,511 + -0.5 289,544 + -0.5 289,545 - +0.4 289,594 + -0.0 289,594 + -0.4 289,731 + -1.4 289,732 - +0.0 289,810 - Pf1N1B4_5530 0.16 -0.5 289,992 + Pf1N1B4_5530 0.45 +0.1 289,992 + Pf1N1B4_5530 0.45 +0.1 289,992 + +0.5 289,992 + Pf1N1B4_5530 0.45 +0.5 289,992 + Pf1N1B4_5530 0.45 +0.1 289,993 - Pf1N1B4_5530 0.45 -0.5 289,993 - Pf1N1B4_5530 0.45 +0.1 290,041 + Pf1N1B4_5530 0.52 +0.1 290,041 + Pf1N1B4_5530 0.52 -1.0 290,041 + Pf1N1B4_5530 0.52 +0.1 290,041 + Pf1N1B4_5530 0.52 +0.5 290,041 + Pf1N1B4_5530 0.52 +0.1 290,041 + Pf1N1B4_5530 0.52 -1.0 290,041 + -0.5 290,041 + Pf1N1B4_5530 0.52 -0.8 290,042 - Pf1N1B4_5530 0.53 -0.5 290,042 - Pf1N1B4_5530 0.53 +1.0 290,042 - +1.5 290,042 - Pf1N1B4_5530 0.53 -1.5 290,042 - Pf1N1B4_5530 0.53 +0.5 290,059 + Pf1N1B4_5530 0.55 -0.5 290,068 - Pf1N1B4_5530 0.57 -0.3 290,095 + Pf1N1B4_5530 0.61 +0.2 290,105 + -1.5 290,106 - Pf1N1B4_5530 0.63 -2.4 290,152 + Pf1N1B4_5530 0.70 +0.3 290,153 - Pf1N1B4_5530 0.70 -0.7 290,153 - Pf1N1B4_5530 0.70 +0.1 290,232 + Pf1N1B4_5530 0.83 -1.5 290,232 + Pf1N1B4_5530 0.83 -0.2 290,232 + Pf1N1B4_5530 0.83 +0.5 290,232 + Pf1N1B4_5530 0.83 +0.5 290,232 + Pf1N1B4_5530 0.83 -0.6 290,233 - Pf1N1B4_5530 0.83 -0.3 290,255 + Pf1N1B4_5530 0.87 -2.2 290,275 + Pf1N1B4_5530 0.90 -0.1 290,275 + Pf1N1B4_5530 0.90 +1.0 290,275 + Pf1N1B4_5530 0.90 +1.4 290,276 - -0.8 290,276 - -2.3 290,276 - -0.3 290,276 - +0.1 290,306 + +0.5 290,306 + -2.2 290,306 + +3.5 290,306 + +0.3 290,306 + -0.8 290,307 - +0.4 290,307 - +0.3 290,307 - +1.0 290,307 - -0.3 290,408 + +0.4 290,419 + -0.4 290,419 + +0.5 290,419 + +0.5 290,419 + -0.3 290,419 + +1.7 290,419 + +0.8 290,419 + -0.7 290,420 - +2.3 290,420 - -0.5 290,420 - +0.5 290,420 - -0.9 290,420 - -0.3 290,420 - +0.5 290,493 + -1.0 290,493 + Pf1N1B4_5531 0.19 -0.5 290,493 + Pf1N1B4_5531 0.19 -0.3 290,493 + Pf1N1B4_5531 0.19 +0.8 290,494 - Pf1N1B4_5531 0.20 +0.5 290,494 - Pf1N1B4_5531 0.20 -0.6 290,494 - Pf1N1B4_5531 0.20 +1.3 290,494 - Pf1N1B4_5531 0.20 +1.2 290,497 + Pf1N1B4_5531 0.20 +0.5 290,498 - Pf1N1B4_5531 0.21 +0.4 290,498 - Pf1N1B4_5531 0.21 +0.3 290,506 + Pf1N1B4_5531 0.23 +1.0 290,772 + -0.0 290,878 + +0.4 290,878 + -0.8 290,878 + +1.1 290,879 - -1.8 290,879 - +0.7 290,925 + +0.1 291,031 - +1.0 291,031 - -0.2 291,031 - -2.0 291,031 - +0.5 291,031 - -0.9 291,037 + -0.7 291,037 + -1.0 291,037 + -0.3 291,055 + -2.3 291,056 - +0.5 291,092 + -2.5 291,092 + +1.2 291,092 + +1.9 291,093 - -0.2 291,093 - +0.4 291,093 - +0.5 291,093 - -0.8 291,093 - +0.0 291,093 - +2.1 291,093 - +1.9 291,097 - -1.0 291,097 - -1.8 291,181 + -0.7 291,182 - -0.5 291,182 - -0.2
Or see this region's nucleotide sequence