Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5460 and Pf1N1B4_5461 are separated by 307 nucleotides Pf1N1B4_5461 and Pf1N1B4_5462 are separated by 559 nucleotides
Pf1N1B4_5460 - TolA protein, at 207,111 to 207,977
_5460
Pf1N1B4_5461 - hypothetical protein, at 208,285 to 208,695
_5461
Pf1N1B4_5462 - hypothetical protein, at 209,255 to 210,247
_5462
Position (kb)
208
209 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 207.515 kb on + strand, within Pf1N1B4_5460 at 207.516 kb on - strand, within Pf1N1B4_5460 at 207.935 kb on + strand at 208.050 kb on + strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.070 kb on + strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.273 kb on - strand at 208.276 kb on + strand at 208.276 kb on + strand at 208.324 kb on + strand at 208.324 kb on + strand at 208.325 kb on - strand at 208.325 kb on - strand at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.411 kb on + strand, within Pf1N1B4_5461 at 208.412 kb on - strand, within Pf1N1B4_5461 at 208.526 kb on - strand, within Pf1N1B4_5461 at 208.553 kb on - strand, within Pf1N1B4_5461 at 208.553 kb on - strand, within Pf1N1B4_5461 at 208.557 kb on - strand, within Pf1N1B4_5461 at 208.557 kb on - strand, within Pf1N1B4_5461 at 208.618 kb on - strand at 208.667 kb on + strand at 208.668 kb on - strand at 208.726 kb on + strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.918 kb on + strand at 208.918 kb on + strand at 208.933 kb on - strand at 208.933 kb on - strand at 208.972 kb on - strand at 208.972 kb on - strand at 208.972 kb on - strand at 208.975 kb on + strand at 208.976 kb on - strand at 208.976 kb on - strand at 208.976 kb on - strand at 209.015 kb on - strand at 209.015 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.141 kb on - strand at 209.228 kb on - strand at 209.231 kb on - strand at 209.231 kb on - strand at 209.237 kb on - strand at 209.287 kb on - strand at 209.318 kb on - strand at 209.318 kb on - strand at 209.351 kb on - strand at 209.351 kb on - strand at 209.351 kb on - strand at 209.369 kb on - strand, within Pf1N1B4_5462 at 209.386 kb on + strand at 209.387 kb on - strand, within Pf1N1B4_5462 at 209.387 kb on - strand at 209.387 kb on - strand at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.554 kb on + strand, within Pf1N1B4_5462 at 209.554 kb on + strand, within Pf1N1B4_5462 at 209.587 kb on - strand, within Pf1N1B4_5462 at 209.587 kb on - strand, within Pf1N1B4_5462 at 209.691 kb on + strand, within Pf1N1B4_5462
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 207,515 + Pf1N1B4_5460 0.47 +0.1 207,516 - Pf1N1B4_5460 0.47 +0.2 207,935 + -1.1 208,050 + -0.5 208,051 - -0.5 208,051 - -0.9 208,051 - -0.2 208,051 - -0.0 208,070 + -1.5 208,071 - -1.3 208,071 - +0.2 208,071 - -0.0 208,071 - +1.7 208,071 - -0.0 208,273 - -0.3 208,276 + +0.5 208,276 + +0.0 208,324 + -2.0 208,324 + -0.2 208,325 - -0.2 208,325 - -0.6 208,377 - Pf1N1B4_5461 0.22 -2.5 208,377 - Pf1N1B4_5461 0.22 +0.3 208,377 - Pf1N1B4_5461 0.22 -2.0 208,377 - Pf1N1B4_5461 0.22 -1.5 208,411 + Pf1N1B4_5461 0.31 +0.1 208,412 - Pf1N1B4_5461 0.31 +1.1 208,526 - Pf1N1B4_5461 0.59 -0.9 208,553 - Pf1N1B4_5461 0.65 -0.5 208,553 - Pf1N1B4_5461 0.65 -0.3 208,557 - Pf1N1B4_5461 0.66 +0.2 208,557 - Pf1N1B4_5461 0.66 -2.2 208,618 - +1.0 208,667 + -0.0 208,668 - -0.9 208,726 + -2.8 208,727 - +0.8 208,727 - -2.5 208,727 - -0.5 208,727 - +0.9 208,918 + +1.9 208,918 + -0.5 208,933 - -0.3 208,933 - -1.9 208,972 - +0.4 208,972 - +0.2 208,972 - -0.5 208,975 + +1.5 208,976 - -0.0 208,976 - -1.0 208,976 - -1.5 209,015 - -0.8 209,015 - +0.7 209,026 - -0.3 209,026 - +0.2 209,026 - -2.5 209,026 - -0.9 209,026 - +1.1 209,141 - +0.1 209,228 - -1.8 209,231 - +3.5 209,231 - -1.3 209,237 - -1.5 209,287 - +2.5 209,318 - -0.9 209,318 - -0.6 209,351 - -1.0 209,351 - +0.1 209,351 - -0.6 209,369 - Pf1N1B4_5462 0.11 +1.3 209,386 + -0.5 209,387 - Pf1N1B4_5462 0.13 -1.8 209,387 - -0.5 209,387 - -0.5 209,511 - Pf1N1B4_5462 0.26 +0.8 209,511 - Pf1N1B4_5462 0.26 -0.3 209,511 - Pf1N1B4_5462 0.26 -0.6 209,511 - Pf1N1B4_5462 0.26 +0.3 209,554 + Pf1N1B4_5462 0.30 -1.3 209,554 + Pf1N1B4_5462 0.30 -0.2 209,587 - Pf1N1B4_5462 0.33 +0.2 209,587 - Pf1N1B4_5462 0.33 -0.4 209,691 + Pf1N1B4_5462 0.44 -0.5
Or see this region's nucleotide sequence