Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5446 and Pf1N1B4_5447 are separated by 46 nucleotides
Pf1N1B4_5446 - hypothetical protein, at 192,136 to 192,315
_5446
Pf1N1B4_5447 - hypothetical protein, at 192,362 to 192,556
_5447
Position (kb)
192
193 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 191.430 kb on + strand at 191.432 kb on + strand at 191.433 kb on - strand at 191.478 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.541 kb on + strand at 191.542 kb on - strand at 191.576 kb on - strand at 191.576 kb on - strand at 191.603 kb on - strand at 191.615 kb on + strand at 191.616 kb on - strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.632 kb on + strand at 191.697 kb on + strand at 191.697 kb on + strand at 191.698 kb on - strand at 191.716 kb on + strand at 191.717 kb on - strand at 191.717 kb on - strand at 191.717 kb on - strand at 191.719 kb on + strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.737 kb on + strand at 191.738 kb on - strand at 191.781 kb on - strand at 191.802 kb on - strand at 191.802 kb on - strand at 191.802 kb on - strand at 191.815 kb on - strand at 191.815 kb on - strand at 191.815 kb on - strand at 191.836 kb on + strand at 191.837 kb on - strand at 191.837 kb on - strand at 191.851 kb on + strand at 191.852 kb on - strand at 191.881 kb on - strand at 191.892 kb on + strand at 191.893 kb on - strand at 191.893 kb on - strand at 191.893 kb on - strand at 191.906 kb on + strand at 191.906 kb on + strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 192.086 kb on - strand at 192.086 kb on - strand at 192.086 kb on - strand at 192.129 kb on - strand at 192.137 kb on + strand at 192.137 kb on + strand at 192.172 kb on - strand, within Pf1N1B4_5446 at 192.229 kb on + strand, within Pf1N1B4_5446 at 192.229 kb on + strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.249 kb on - strand, within Pf1N1B4_5446 at 192.387 kb on + strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.412 kb on + strand, within Pf1N1B4_5447 at 192.441 kb on - strand at 192.441 kb on - strand, within Pf1N1B4_5447 at 192.441 kb on - strand at 192.540 kb on + strand at 192.540 kb on + strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.551 kb on + strand at 192.552 kb on - strand at 192.552 kb on - strand at 192.571 kb on - strand at 192.573 kb on + strand at 192.574 kb on - strand at 192.574 kb on - strand at 192.635 kb on - strand at 192.671 kb on - strand at 192.671 kb on - strand at 192.671 kb on - strand at 192.677 kb on + strand at 192.677 kb on + strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.740 kb on + strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.853 kb on - strand at 192.853 kb on - strand at 192.853 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.934 kb on - strand at 192.934 kb on - strand at 192.945 kb on - strand at 192.945 kb on - strand at 192.945 kb on - strand at 193.010 kb on - strand at 193.066 kb on - strand at 193.066 kb on - strand at 193.123 kb on + strand at 193.146 kb on + strand at 193.147 kb on - strand at 193.147 kb on - strand at 193.170 kb on - strand at 193.170 kb on - strand at 193.208 kb on + strand at 193.209 kb on - strand at 193.209 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.329 kb on - strand at 193.329 kb on - strand at 193.329 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.340 kb on + strand at 193.341 kb on - strand at 193.341 kb on - strand at 193.341 kb on - strand at 193.369 kb on - strand at 193.383 kb on + strand at 193.399 kb on + strand at 193.449 kb on + strand at 193.450 kb on - strand at 193.552 kb on - strand at 193.552 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 191,430 + +2.3 191,432 + -0.1 191,433 - +0.7 191,478 + +0.7 191,537 + +2.9 191,537 + -1.3 191,537 + +0.7 191,537 + +0.7 191,537 + +0.9 191,537 + +0.5 191,537 + +1.1 191,538 - +1.5 191,538 - +0.5 191,538 - +0.3 191,538 - -0.1 191,538 - -0.5 191,538 - -3.0 191,541 + +1.5 191,542 - +0.1 191,576 - +0.9 191,576 - +0.1 191,603 - -1.8 191,615 + -0.3 191,616 - +0.4 191,628 + +1.5 191,628 + +1.3 191,628 + +1.0 191,628 + +0.6 191,628 + +0.5 191,628 + +0.1 191,628 + +0.9 191,629 - +0.1 191,629 - -0.2 191,629 - -0.2 191,629 - +2.1 191,632 + +0.5 191,697 + -0.4 191,697 + +0.4 191,698 - -1.9 191,716 + -1.1 191,717 - -1.0 191,717 - -0.8 191,717 - -2.6 191,719 + +0.2 191,720 - +0.1 191,720 - -0.3 191,720 - +1.0 191,720 - -0.5 191,720 - +0.5 191,737 + +1.3 191,738 - -2.6 191,781 - +0.9 191,802 - +0.7 191,802 - +0.3 191,802 - +0.5 191,815 - +1.1 191,815 - +0.0 191,815 - -1.3 191,836 + +0.2 191,837 - -0.7 191,837 - -1.6 191,851 + +0.1 191,852 - -1.6 191,881 - -1.8 191,892 + -0.5 191,893 - -0.5 191,893 - +0.5 191,893 - -1.5 191,906 + +1.7 191,906 + -0.5 191,907 - -0.1 191,907 - +0.1 191,907 - -1.0 191,907 - +0.0 191,927 + +0.3 191,927 + -0.9 191,927 + -0.3 191,927 + -0.2 191,927 + +0.7 191,927 + -2.0 191,928 - -0.8 191,928 - -0.5 191,928 - -1.0 191,928 - +1.3 191,928 - -0.8 191,928 - +1.3 191,928 - +1.5 191,928 - -0.9 191,928 - +0.3 191,928 - +0.4 191,928 - -0.6 191,928 - -0.0 192,086 - -1.0 192,086 - -1.6 192,086 - +0.7 192,129 - -0.6 192,137 + -0.2 192,137 + -1.5 192,172 - Pf1N1B4_5446 0.20 -1.3 192,229 + Pf1N1B4_5446 0.52 -1.9 192,229 + Pf1N1B4_5446 0.52 -0.8 192,230 - Pf1N1B4_5446 0.52 -0.1 192,230 - Pf1N1B4_5446 0.52 -1.5 192,230 - Pf1N1B4_5446 0.52 -2.5 192,249 - Pf1N1B4_5446 0.63 -1.4 192,387 + Pf1N1B4_5447 0.13 -1.3 192,388 - Pf1N1B4_5447 0.13 -2.6 192,388 - Pf1N1B4_5447 0.13 -1.5 192,388 - Pf1N1B4_5447 0.13 +0.1 192,388 - Pf1N1B4_5447 0.13 -0.5 192,412 + Pf1N1B4_5447 0.26 +0.3 192,441 - -0.5 192,441 - Pf1N1B4_5447 0.41 -0.7 192,441 - -0.5 192,540 + -0.3 192,540 + -1.0 192,541 - +0.3 192,541 - +0.1 192,541 - -0.3 192,541 - -1.0 192,541 - +0.5 192,551 + +0.5 192,552 - +0.0 192,552 - -0.0 192,571 - -0.3 192,573 + -0.1 192,574 - -0.0 192,574 - -1.0 192,635 - +1.3 192,671 - +0.1 192,671 - -0.8 192,671 - -0.8 192,677 + +0.7 192,677 + +0.2 192,678 - -1.0 192,678 - +0.1 192,678 - +0.9 192,678 - +0.3 192,678 - +0.9 192,678 - -0.2 192,678 - +0.1 192,678 - +0.5 192,678 - -0.6 192,678 - -0.7 192,678 - -1.3 192,678 - +0.1 192,678 - -0.4 192,678 - -0.3 192,678 - -0.5 192,678 - +1.0 192,678 - +0.7 192,678 - +0.2 192,678 - +0.7 192,678 - -1.4 192,733 - -0.1 192,733 - -1.8 192,733 - +1.2 192,733 - -1.7 192,740 + +0.9 192,741 - +1.4 192,741 - -0.5 192,741 - -0.7 192,741 - +0.3 192,741 - -0.4 192,761 + +0.4 192,761 + +1.9 192,761 + +1.3 192,761 + -1.0 192,762 - +1.5 192,762 - +0.4 192,762 - -0.8 192,762 - +0.7 192,762 - +0.1 192,762 - -0.1 192,762 - -0.2 192,762 - -0.5 192,762 - +1.5 192,762 - -0.9 192,762 - +0.2 192,762 - -0.7 192,762 - +0.8 192,762 - +0.5 192,762 - +0.1 192,762 - +0.6 192,762 - -1.5 192,762 - -0.5 192,762 - -0.3 192,762 - -0.9 192,762 - +2.1 192,853 - +1.3 192,853 - -0.2 192,853 - +0.3 192,863 - -0.4 192,863 - -0.5 192,863 - +0.3 192,863 - +0.3 192,863 - +0.9 192,863 - +1.5 192,863 - +0.2 192,863 - +0.0 192,863 - +0.1 192,863 - -0.1 192,863 - -1.0 192,934 - -0.8 192,934 - -1.0 192,945 - -0.0 192,945 - +0.3 192,945 - +0.2 193,010 - -1.0 193,066 - +0.5 193,066 - -1.0 193,123 + +0.7 193,146 + -0.3 193,147 - -1.6 193,147 - +0.5 193,170 - +0.4 193,170 - -0.7 193,208 + +0.6 193,209 - +0.9 193,209 - -2.0 193,267 - +0.2 193,267 - +0.1 193,267 - -0.1 193,267 - -0.8 193,329 - -0.2 193,329 - +0.5 193,329 - +1.0 193,335 - +1.0 193,335 - +0.3 193,335 - +0.5 193,335 - -1.0 193,335 - -0.2 193,335 - -2.9 193,335 - +1.0 193,335 - -0.5 193,335 - +0.5 193,340 + +0.0 193,341 - -0.5 193,341 - -0.7 193,341 - +1.5 193,369 - +1.5 193,383 + -0.9 193,399 + +1.0 193,449 + +0.3 193,450 - -0.7 193,552 - +1.0 193,552 - +0.3
Or see this region's nucleotide sequence