Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5132 and Pf1N1B4_5133 are separated by 131 nucleotides Pf1N1B4_5133 and Pf1N1B4_5134 are separated by 13 nucleotides Pf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotides
Pf1N1B4_5132 - Transcriptional regulator, AraC family, at 5,710,848 to 5,711,864
_5132
Pf1N1B4_5133 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 5,711,996 to 5,713,180
_5133
Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045
_5134
Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685
_5135
Position (kb) 5711
5712
5713
5714 Strain fitness (log2 ratio) -2
-1
0
1 at 5711.686 kb on - strand at 5711.742 kb on - strand, within Pf1N1B4_5132 at 5711.827 kb on + strand at 5711.932 kb on - strand at 5711.954 kb on + strand at 5711.980 kb on + strand at 5711.980 kb on + strand at 5711.980 kb on + strand at 5711.981 kb on - strand at 5712.194 kb on + strand, within Pf1N1B4_5133 at 5712.194 kb on + strand, within Pf1N1B4_5133 at 5712.194 kb on + strand, within Pf1N1B4_5133 at 5712.195 kb on - strand, within Pf1N1B4_5133 at 5712.371 kb on + strand, within Pf1N1B4_5133 at 5712.428 kb on + strand, within Pf1N1B4_5133 at 5712.476 kb on - strand at 5712.635 kb on - strand, within Pf1N1B4_5133 at 5713.718 kb on + strand, within Pf1N1B4_5134 at 5713.936 kb on - strand, within Pf1N1B4_5134 at 5714.038 kb on - strand at 5714.168 kb on - strand, within Pf1N1B4_5135
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 5,711,686 - -0.5 5,711,742 - Pf1N1B4_5132 0.88 -1.2 5,711,827 + +0.9 5,711,932 - +0.1 5,711,954 + -2.5 5,711,980 + +0.2 5,711,980 + +0.3 5,711,980 + -1.5 5,711,981 - -0.0 5,712,194 + Pf1N1B4_5133 0.17 -1.1 5,712,194 + Pf1N1B4_5133 0.17 -0.8 5,712,194 + Pf1N1B4_5133 0.17 -0.2 5,712,195 - Pf1N1B4_5133 0.17 +0.0 5,712,371 + Pf1N1B4_5133 0.32 -0.1 5,712,428 + Pf1N1B4_5133 0.36 -2.4 5,712,476 - +0.5 5,712,635 - Pf1N1B4_5133 0.54 -1.1 5,713,718 + Pf1N1B4_5134 0.62 +0.5 5,713,936 - Pf1N1B4_5134 0.87 +1.7 5,714,038 - -0.5 5,714,168 - Pf1N1B4_5135 0.20 -0.5
Or see this region's nucleotide sequence