Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5004 and Pf1N1B4_5005 are separated by 285 nucleotides Pf1N1B4_5005 and Pf1N1B4_5006 are separated by 103 nucleotides
Pf1N1B4_5004 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,554,702 to 5,555,649
_5004
Pf1N1B4_5005 - LysR family transcriptional regulator STM2281, at 5,555,935 to 5,556,807
_5005
Pf1N1B4_5006 - Rtn protein, at 5,556,911 to 5,558,446
_5006
Position (kb) 5555
5556
5557 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5555.112 kb on - strand, within Pf1N1B4_5004 at 5555.348 kb on + strand at 5555.348 kb on + strand, within Pf1N1B4_5004 at 5555.348 kb on + strand at 5555.349 kb on - strand, within Pf1N1B4_5004 at 5555.349 kb on - strand, within Pf1N1B4_5004 at 5555.349 kb on - strand at 5555.360 kb on + strand, within Pf1N1B4_5004 at 5555.378 kb on + strand, within Pf1N1B4_5004 at 5555.379 kb on - strand, within Pf1N1B4_5004 at 5555.379 kb on - strand, within Pf1N1B4_5004 at 5555.639 kb on + strand at 5555.640 kb on - strand at 5555.643 kb on + strand at 5555.643 kb on + strand at 5555.643 kb on + strand at 5555.644 kb on - strand at 5556.068 kb on + strand at 5556.068 kb on + strand, within Pf1N1B4_5005 at 5556.129 kb on - strand, within Pf1N1B4_5005 at 5556.358 kb on - strand, within Pf1N1B4_5005 at 5556.488 kb on + strand at 5556.507 kb on - strand at 5556.549 kb on + strand, within Pf1N1B4_5005 at 5556.549 kb on + strand, within Pf1N1B4_5005 at 5556.815 kb on - strand at 5556.832 kb on + strand at 5556.833 kb on - strand at 5556.833 kb on - strand at 5557.010 kb on + strand at 5557.010 kb on + strand at 5557.119 kb on + strand, within Pf1N1B4_5006 at 5557.120 kb on - strand, within Pf1N1B4_5006 at 5557.212 kb on + strand, within Pf1N1B4_5006 at 5557.213 kb on - strand, within Pf1N1B4_5006 at 5557.230 kb on - strand, within Pf1N1B4_5006 at 5557.254 kb on - strand, within Pf1N1B4_5006 at 5557.296 kb on + strand, within Pf1N1B4_5006 at 5557.296 kb on + strand, within Pf1N1B4_5006 at 5557.297 kb on - strand, within Pf1N1B4_5006 at 5557.452 kb on + strand at 5557.537 kb on + strand, within Pf1N1B4_5006 at 5557.592 kb on + strand, within Pf1N1B4_5006 at 5557.592 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 5,555,112 - Pf1N1B4_5004 0.43 -0.2 5,555,348 + +0.5 5,555,348 + Pf1N1B4_5004 0.68 +0.9 5,555,348 + -0.5 5,555,349 - Pf1N1B4_5004 0.68 -0.5 5,555,349 - Pf1N1B4_5004 0.68 -1.1 5,555,349 - -1.9 5,555,360 + Pf1N1B4_5004 0.69 +2.0 5,555,378 + Pf1N1B4_5004 0.71 -0.1 5,555,379 - Pf1N1B4_5004 0.71 +0.9 5,555,379 - Pf1N1B4_5004 0.71 +0.2 5,555,639 + +0.3 5,555,640 - -0.2 5,555,643 + -0.5 5,555,643 + -1.5 5,555,643 + -1.4 5,555,644 - +0.1 5,556,068 + +0.9 5,556,068 + Pf1N1B4_5005 0.15 -0.6 5,556,129 - Pf1N1B4_5005 0.22 +0.5 5,556,358 - Pf1N1B4_5005 0.48 -0.1 5,556,488 + +0.5 5,556,507 - +0.2 5,556,549 + Pf1N1B4_5005 0.70 -3.1 5,556,549 + Pf1N1B4_5005 0.70 -0.4 5,556,815 - -1.1 5,556,832 + -2.4 5,556,833 - -0.5 5,556,833 - -1.5 5,557,010 + -0.3 5,557,010 + -1.5 5,557,119 + Pf1N1B4_5006 0.14 +2.3 5,557,120 - Pf1N1B4_5006 0.14 -0.0 5,557,212 + Pf1N1B4_5006 0.20 -0.5 5,557,213 - Pf1N1B4_5006 0.20 +3.3 5,557,230 - Pf1N1B4_5006 0.21 -1.1 5,557,254 - Pf1N1B4_5006 0.22 -1.0 5,557,296 + Pf1N1B4_5006 0.25 -0.5 5,557,296 + Pf1N1B4_5006 0.25 +2.3 5,557,297 - Pf1N1B4_5006 0.25 -0.1 5,557,452 + -1.5 5,557,537 + Pf1N1B4_5006 0.41 +0.9 5,557,592 + Pf1N1B4_5006 0.44 -1.1 5,557,592 + -0.1
Or see this region's nucleotide sequence