Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4812 and Pf1N1B4_4813 are separated by 9 nucleotides Pf1N1B4_4813 and Pf1N1B4_4814 overlap by 7 nucleotides Pf1N1B4_4814 and Pf1N1B4_4815 overlap by 4 nucleotides
Pf1N1B4_4812 - Site-specific recombinase, phage integrase family, at 5,314,721 to 5,315,692
_4812
Pf1N1B4_4813 - Iron(III) dicitrate transport ATP-binding protein FecE (TC 3.A.1.14.1), at 5,315,702 to 5,316,457
_4813
Pf1N1B4_4814 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 5,316,451 to 5,317,512
_4814
Pf1N1B4_4815 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 5,317,509 to 5,318,693
_4815
Position (kb) 5316
5317
5318 Strain fitness (log2 ratio) -1
0
1
2 at 5315.483 kb on + strand, within Pf1N1B4_4812 at 5315.486 kb on + strand, within Pf1N1B4_4812 at 5315.486 kb on + strand, within Pf1N1B4_4812 at 5315.486 kb on + strand at 5315.487 kb on - strand at 5315.636 kb on + strand at 5315.636 kb on + strand at 5315.637 kb on - strand at 5315.637 kb on - strand at 5315.637 kb on - strand at 5315.773 kb on - strand at 5315.784 kb on - strand, within Pf1N1B4_4813 at 5316.237 kb on + strand, within Pf1N1B4_4813 at 5316.536 kb on - strand at 5317.103 kb on + strand, within Pf1N1B4_4814 at 5317.103 kb on + strand, within Pf1N1B4_4814 at 5317.104 kb on - strand, within Pf1N1B4_4814 at 5317.104 kb on - strand at 5317.470 kb on - strand at 5317.492 kb on + strand at 5317.492 kb on + strand at 5317.493 kb on - strand at 5317.540 kb on + strand at 5317.666 kb on + strand, within Pf1N1B4_4815 at 5317.678 kb on + strand, within Pf1N1B4_4815 at 5318.201 kb on - strand, within Pf1N1B4_4815 at 5318.239 kb on + strand, within Pf1N1B4_4815 at 5318.482 kb on + strand, within Pf1N1B4_4815
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 5,315,483 + Pf1N1B4_4812 0.78 +0.0 5,315,486 + Pf1N1B4_4812 0.79 -0.1 5,315,486 + Pf1N1B4_4812 0.79 -0.6 5,315,486 + +1.4 5,315,487 - +0.4 5,315,636 + -0.3 5,315,636 + -0.7 5,315,637 - +1.3 5,315,637 - -0.8 5,315,637 - +0.3 5,315,773 - +2.0 5,315,784 - Pf1N1B4_4813 0.11 -0.4 5,316,237 + Pf1N1B4_4813 0.71 -0.6 5,316,536 - +0.9 5,317,103 + Pf1N1B4_4814 0.61 -0.1 5,317,103 + Pf1N1B4_4814 0.61 -1.0 5,317,104 - Pf1N1B4_4814 0.61 -0.8 5,317,104 - -0.9 5,317,470 - +0.0 5,317,492 + -0.6 5,317,492 + +1.0 5,317,493 - +0.1 5,317,540 + -0.5 5,317,666 + Pf1N1B4_4815 0.13 +1.0 5,317,678 + Pf1N1B4_4815 0.14 +0.4 5,318,201 - Pf1N1B4_4815 0.58 +0.7 5,318,239 + Pf1N1B4_4815 0.62 +0.2 5,318,482 + Pf1N1B4_4815 0.82 +0.4
Or see this region's nucleotide sequence