Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4458 and Pf1N1B4_4459 overlap by 11 nucleotides Pf1N1B4_4459 and Pf1N1B4_4460 are separated by 187 nucleotides Pf1N1B4_4460 and Pf1N1B4_4461 are separated by 142 nucleotides
Pf1N1B4_4458 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,918,049 to 4,919,530
_4458
Pf1N1B4_4459 - Thermostable hemolysin delta-VPH, at 4,919,520 to 4,920,197
_4459
Pf1N1B4_4460 - Cytochrome b561, at 4,920,385 to 4,920,957
_4460
Pf1N1B4_4461 - Glutathione-regulated potassium-efflux system protein KefB, at 4,921,100 to 4,922,908
_4461
Position (kb)
4920
4921 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4919.430 kb on + strand at 4919.430 kb on + strand at 4919.431 kb on - strand at 4919.648 kb on - strand, within Pf1N1B4_4459 at 4919.797 kb on + strand, within Pf1N1B4_4459 at 4919.797 kb on + strand at 4919.797 kb on + strand at 4919.798 kb on - strand, within Pf1N1B4_4459 at 4920.235 kb on - strand at 4920.285 kb on - strand at 4920.384 kb on + strand at 4920.384 kb on + strand at 4920.385 kb on - strand at 4920.404 kb on + strand at 4920.404 kb on + strand at 4920.469 kb on + strand, within Pf1N1B4_4460 at 4920.673 kb on + strand, within Pf1N1B4_4460 at 4920.743 kb on - strand, within Pf1N1B4_4460 at 4921.049 kb on + strand at 4921.050 kb on - strand at 4921.050 kb on - strand at 4921.050 kb on - strand at 4921.050 kb on - strand at 4921.204 kb on - strand at 4921.204 kb on - strand at 4921.333 kb on - strand, within Pf1N1B4_4461 at 4921.546 kb on - strand, within Pf1N1B4_4461 at 4921.828 kb on - strand, within Pf1N1B4_4461
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 4,919,430 + -0.1 4,919,430 + -0.5 4,919,431 - -0.5 4,919,648 - Pf1N1B4_4459 0.19 -0.0 4,919,797 + Pf1N1B4_4459 0.41 -0.5 4,919,797 + +1.7 4,919,797 + +0.1 4,919,798 - Pf1N1B4_4459 0.41 +0.8 4,920,235 - -2.9 4,920,285 - +0.2 4,920,384 + +1.5 4,920,384 + +1.9 4,920,385 - -1.3 4,920,404 + +0.2 4,920,404 + -0.3 4,920,469 + Pf1N1B4_4460 0.15 -0.8 4,920,673 + Pf1N1B4_4460 0.50 +1.5 4,920,743 - Pf1N1B4_4460 0.62 -2.0 4,921,049 + +0.1 4,921,050 - +3.3 4,921,050 - -0.2 4,921,050 - +0.0 4,921,050 - +0.2 4,921,204 - +0.7 4,921,204 - +0.1 4,921,333 - Pf1N1B4_4461 0.13 -1.5 4,921,546 - Pf1N1B4_4461 0.25 -0.8 4,921,828 - Pf1N1B4_4461 0.40 +0.2
Or see this region's nucleotide sequence