Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4315 and Pf1N1B4_4316 are separated by 212 nucleotides Pf1N1B4_4316 and Pf1N1B4_4317 are separated by 421 nucleotides Pf1N1B4_4317 and Pf1N1B4_4318 are separated by 147 nucleotides
Pf1N1B4_4315 - hypothetical protein, at 4,777,170 to 4,777,451
_4315
Pf1N1B4_4316 - Ribosomal protein S3AE, at 4,777,664 to 4,777,999
_4316
Pf1N1B4_4317 - DNA/RNA non-specific endonuclease, at 4,778,421 to 4,779,251
_4317
Pf1N1B4_4318 - hypothetical protein, at 4,779,399 to 4,780,760
_4318
Position (kb)
4778
4779
4780 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4777.447 kb on - strand at 4777.580 kb on - strand at 4777.641 kb on + strand at 4777.872 kb on + strand, within Pf1N1B4_4316 at 4777.873 kb on - strand, within Pf1N1B4_4316 at 4778.079 kb on - strand at 4778.087 kb on - strand at 4778.134 kb on + strand at 4778.184 kb on - strand at 4778.222 kb on + strand at 4778.223 kb on - strand at 4778.296 kb on + strand at 4778.374 kb on - strand at 4778.701 kb on + strand, within Pf1N1B4_4317 at 4779.129 kb on + strand, within Pf1N1B4_4317 at 4779.129 kb on + strand, within Pf1N1B4_4317 at 4779.133 kb on + strand at 4779.133 kb on + strand at 4779.175 kb on - strand at 4779.175 kb on - strand at 4779.317 kb on - strand at 4779.743 kb on - strand at 4779.918 kb on - strand, within Pf1N1B4_4318 at 4779.924 kb on - strand, within Pf1N1B4_4318 at 4780.096 kb on + strand, within Pf1N1B4_4318 at 4780.096 kb on + strand, within Pf1N1B4_4318 at 4780.097 kb on - strand, within Pf1N1B4_4318 at 4780.226 kb on - strand, within Pf1N1B4_4318
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 4,777,447 - +2.1 4,777,580 - +0.7 4,777,641 + -0.0 4,777,872 + Pf1N1B4_4316 0.62 +0.3 4,777,873 - Pf1N1B4_4316 0.62 -2.0 4,778,079 - +1.0 4,778,087 - -0.4 4,778,134 + -1.0 4,778,184 - -0.2 4,778,222 + -1.1 4,778,223 - -0.9 4,778,296 + -2.6 4,778,374 - -1.9 4,778,701 + Pf1N1B4_4317 0.34 -1.8 4,779,129 + Pf1N1B4_4317 0.85 -0.4 4,779,129 + Pf1N1B4_4317 0.85 +0.2 4,779,133 + +2.1 4,779,133 + -1.0 4,779,175 - +0.6 4,779,175 - +0.5 4,779,317 - -0.8 4,779,743 - +1.9 4,779,918 - Pf1N1B4_4318 0.38 +0.6 4,779,924 - Pf1N1B4_4318 0.39 +0.1 4,780,096 + Pf1N1B4_4318 0.51 +0.8 4,780,096 + Pf1N1B4_4318 0.51 -1.4 4,780,097 - Pf1N1B4_4318 0.51 -0.2 4,780,226 - Pf1N1B4_4318 0.61 -0.4
Or see this region's nucleotide sequence