Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3774 and Pf1N1B4_3775 are separated by 75 nucleotides Pf1N1B4_3775 and Pf1N1B4_3776 are separated by 131 nucleotides Pf1N1B4_3776 and Pf1N1B4_3777 are separated by 238 nucleotides
Pf1N1B4_3774 - Smr domain protein, at 4,184,144 to 4,184,701
_3774
Pf1N1B4_3775 - FIG00956876: hypothetical protein, at 4,184,777 to 4,185,097
_3775
Pf1N1B4_3776 - Isochorismatase (EC 3.3.2.1), at 4,185,229 to 4,185,819
_3776
Pf1N1B4_3777 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 4,186,058 to 4,186,966
_3777
Position (kb)
4185
4186 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4185.893 kb on + strand at 4185.893 kb on + strand at 4185.894 kb on - strand at 4185.947 kb on - strand at 4185.947 kb on - strand at 4185.963 kb on - strand at 4185.973 kb on + strand at 4185.973 kb on + strand at 4185.974 kb on - strand at 4185.974 kb on - strand at 4185.974 kb on - strand at 4186.023 kb on + strand at 4186.094 kb on - strand at 4186.219 kb on + strand, within Pf1N1B4_3777 at 4186.219 kb on + strand, within Pf1N1B4_3777 at 4186.219 kb on + strand, within Pf1N1B4_3777 at 4186.219 kb on + strand, within Pf1N1B4_3777 at 4186.219 kb on + strand, within Pf1N1B4_3777 at 4186.220 kb on - strand, within Pf1N1B4_3777 at 4186.220 kb on - strand, within Pf1N1B4_3777 at 4186.223 kb on + strand, within Pf1N1B4_3777 at 4186.223 kb on + strand at 4186.223 kb on + strand, within Pf1N1B4_3777 at 4186.223 kb on + strand at 4186.223 kb on + strand, within Pf1N1B4_3777 at 4186.223 kb on + strand at 4186.224 kb on - strand, within Pf1N1B4_3777 at 4186.310 kb on + strand, within Pf1N1B4_3777 at 4186.339 kb on + strand, within Pf1N1B4_3777 at 4186.446 kb on + strand, within Pf1N1B4_3777 at 4186.446 kb on + strand, within Pf1N1B4_3777 at 4186.447 kb on - strand, within Pf1N1B4_3777 at 4186.447 kb on - strand, within Pf1N1B4_3777
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 4,184,331 - Pf1N1B4_3774 0.34 -1.0 4,184,331 - Pf1N1B4_3774 0.34 +1.3 4,184,872 - Pf1N1B4_3775 0.30 -1.9 4,184,872 - Pf1N1B4_3775 0.30 -0.8 4,184,872 - Pf1N1B4_3775 0.30 -0.6 4,185,893 + +2.7 4,185,893 + -1.4 4,185,894 - -1.1 4,185,947 - +0.0 4,185,947 - -0.5 4,185,963 - +1.5 4,185,973 + -1.1 4,185,973 + +0.3 4,185,974 - -0.1 4,185,974 - +0.0 4,185,974 - -1.1 4,186,023 + -1.1 4,186,094 - +0.9 4,186,219 + Pf1N1B4_3777 0.18 -1.1 4,186,219 + Pf1N1B4_3777 0.18 -0.4 4,186,219 + Pf1N1B4_3777 0.18 +1.2 4,186,219 + Pf1N1B4_3777 0.18 +0.9 4,186,219 + Pf1N1B4_3777 0.18 -0.1 4,186,220 - Pf1N1B4_3777 0.18 -0.7 4,186,220 - Pf1N1B4_3777 0.18 +0.3 4,186,223 + Pf1N1B4_3777 0.18 -1.4 4,186,223 + +0.9 4,186,223 + Pf1N1B4_3777 0.18 -1.9 4,186,223 + +2.5 4,186,223 + Pf1N1B4_3777 0.18 +0.2 4,186,223 + -0.7 4,186,224 - Pf1N1B4_3777 0.18 -1.4 4,186,310 + Pf1N1B4_3777 0.28 +0.9 4,186,339 + Pf1N1B4_3777 0.31 -1.1 4,186,446 + Pf1N1B4_3777 0.43 -0.4 4,186,446 + Pf1N1B4_3777 0.43 -0.4 4,186,447 - Pf1N1B4_3777 0.43 -0.3 4,186,447 - Pf1N1B4_3777 0.43 -0.5
Or see this region's nucleotide sequence