Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3295 and Pf1N1B4_5219 are separated by 333 nucleotides Pf1N1B4_5219 and Pf1N1B4_3296 are separated by 181 nucleotides Pf1N1B4_3296 and Pf1N1B4_3297 are separated by 314 nucleotides
Pf1N1B4_3295 - Filamentation induced by cAMP protein Fic, at 3,681,228 to 3,682,793
_3295
Pf1N1B4_5219 - tRNA-Gly-CCC, at 3,683,127 to 3,683,197
_5219
Pf1N1B4_3296 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 3,683,379 to 3,684,263
_3296
Pf1N1B4_3297 - Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70), at 3,684,578 to 3,685,270
_3297
Position (kb)
3683
3684
3685 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3682.405 kb on + strand, within Pf1N1B4_3295 at 3682.405 kb on + strand, within Pf1N1B4_3295 at 3682.406 kb on - strand, within Pf1N1B4_3295 at 3682.406 kb on - strand, within Pf1N1B4_3295 at 3682.422 kb on + strand, within Pf1N1B4_3295 at 3682.432 kb on + strand, within Pf1N1B4_3295 at 3682.433 kb on - strand, within Pf1N1B4_3295 at 3682.510 kb on + strand, within Pf1N1B4_3295 at 3682.561 kb on - strand, within Pf1N1B4_3295 at 3682.561 kb on - strand, within Pf1N1B4_3295 at 3682.606 kb on + strand, within Pf1N1B4_3295 at 3682.607 kb on - strand, within Pf1N1B4_3295 at 3682.707 kb on + strand at 3682.707 kb on + strand at 3682.707 kb on + strand at 3682.707 kb on + strand at 3682.867 kb on - strand at 3682.879 kb on - strand at 3682.972 kb on - strand at 3683.078 kb on - strand at 3683.176 kb on - strand, within Pf1N1B4_5219 at 3683.185 kb on - strand, within Pf1N1B4_5219 at 3683.186 kb on + strand, within Pf1N1B4_5219 at 3683.186 kb on + strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand at 3683.187 kb on - strand at 3683.265 kb on + strand at 3683.265 kb on + strand at 3683.326 kb on + strand at 3683.411 kb on - strand at 3683.411 kb on - strand at 3683.411 kb on - strand at 3683.590 kb on + strand at 3683.590 kb on + strand, within Pf1N1B4_3296 at 3683.590 kb on + strand, within Pf1N1B4_3296 at 3683.591 kb on - strand, within Pf1N1B4_3296 at 3683.591 kb on - strand, within Pf1N1B4_3296 at 3683.653 kb on + strand, within Pf1N1B4_3296 at 3683.740 kb on + strand, within Pf1N1B4_3296 at 3683.740 kb on + strand, within Pf1N1B4_3296 at 3683.831 kb on - strand at 3683.851 kb on + strand, within Pf1N1B4_3296 at 3684.088 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.214 kb on + strand at 3684.234 kb on + strand at 3684.234 kb on + strand at 3684.234 kb on + strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.236 kb on + strand at 3684.236 kb on + strand at 3684.268 kb on + strand at 3684.269 kb on - strand at 3684.326 kb on + strand at 3684.326 kb on + strand at 3684.327 kb on - strand at 3684.332 kb on + strand at 3684.333 kb on - strand at 3684.333 kb on - strand at 3684.333 kb on - strand at 3684.391 kb on + strand at 3684.391 kb on + strand at 3684.391 kb on + strand at 3684.392 kb on - strand at 3684.392 kb on - strand at 3684.392 kb on - strand at 3684.393 kb on + strand at 3684.393 kb on + strand at 3684.393 kb on + strand at 3684.394 kb on - strand at 3684.394 kb on - strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.461 kb on - strand at 3684.533 kb on - strand at 3684.579 kb on + strand at 3684.580 kb on - strand at 3684.677 kb on + strand, within Pf1N1B4_3297 at 3684.713 kb on - strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.876 kb on + strand, within Pf1N1B4_3297 at 3684.876 kb on + strand, within Pf1N1B4_3297 at 3684.877 kb on - strand at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.226 kb on - strand at 3685.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 3,682,405 + Pf1N1B4_3295 0.75 +0.6 3,682,405 + Pf1N1B4_3295 0.75 +0.3 3,682,406 - Pf1N1B4_3295 0.75 +0.3 3,682,406 - Pf1N1B4_3295 0.75 -0.0 3,682,422 + Pf1N1B4_3295 0.76 -0.6 3,682,432 + Pf1N1B4_3295 0.77 +1.2 3,682,433 - Pf1N1B4_3295 0.77 -2.0 3,682,510 + Pf1N1B4_3295 0.82 -1.3 3,682,561 - Pf1N1B4_3295 0.85 +0.5 3,682,561 - Pf1N1B4_3295 0.85 -1.3 3,682,606 + Pf1N1B4_3295 0.88 -0.9 3,682,607 - Pf1N1B4_3295 0.88 +1.7 3,682,707 + -1.0 3,682,707 + +0.0 3,682,707 + +0.0 3,682,707 + +0.0 3,682,867 - +1.4 3,682,879 - +0.2 3,682,972 - -1.3 3,683,078 - -0.6 3,683,176 - Pf1N1B4_5219 0.69 -0.8 3,683,185 - Pf1N1B4_5219 0.82 -1.6 3,683,186 + Pf1N1B4_5219 0.83 -1.1 3,683,186 + Pf1N1B4_5219 0.83 +0.4 3,683,187 - Pf1N1B4_5219 0.85 +1.0 3,683,187 - Pf1N1B4_5219 0.85 +1.8 3,683,187 - Pf1N1B4_5219 0.85 -0.8 3,683,187 - Pf1N1B4_5219 0.85 -1.0 3,683,187 - +0.0 3,683,187 - +2.0 3,683,265 + +0.2 3,683,265 + +0.6 3,683,326 + -1.8 3,683,411 - +0.9 3,683,411 - +0.8 3,683,411 - -0.6 3,683,590 + -1.0 3,683,590 + Pf1N1B4_3296 0.24 -0.5 3,683,590 + Pf1N1B4_3296 0.24 -1.6 3,683,591 - Pf1N1B4_3296 0.24 -1.6 3,683,591 - Pf1N1B4_3296 0.24 +1.0 3,683,653 + Pf1N1B4_3296 0.31 -0.6 3,683,740 + Pf1N1B4_3296 0.41 +0.4 3,683,740 + Pf1N1B4_3296 0.41 -0.6 3,683,831 - +0.6 3,683,851 + Pf1N1B4_3296 0.53 -0.5 3,684,088 + Pf1N1B4_3296 0.80 -1.3 3,684,173 + Pf1N1B4_3296 0.90 -0.8 3,684,173 + Pf1N1B4_3296 0.90 -0.2 3,684,173 + Pf1N1B4_3296 0.90 +0.2 3,684,214 + +2.4 3,684,234 + -1.0 3,684,234 + +0.9 3,684,234 + +0.6 3,684,235 - +0.6 3,684,235 - +0.4 3,684,235 - -0.5 3,684,235 - +0.4 3,684,235 - +0.3 3,684,235 - +0.9 3,684,236 + +0.3 3,684,236 + +0.7 3,684,268 + -2.0 3,684,269 - +0.4 3,684,326 + +0.1 3,684,326 + +0.2 3,684,327 - +0.7 3,684,332 + -0.8 3,684,333 - -0.6 3,684,333 - -0.3 3,684,333 - -2.3 3,684,391 + +0.7 3,684,391 + -1.0 3,684,391 + +2.0 3,684,392 - +0.6 3,684,392 - -1.1 3,684,392 - +0.5 3,684,393 + -1.6 3,684,393 + -1.3 3,684,393 + +0.0 3,684,394 - -1.0 3,684,394 - +0.0 3,684,455 + -1.2 3,684,455 + +1.4 3,684,455 + -0.2 3,684,455 + +0.0 3,684,461 - -0.2 3,684,533 - +0.0 3,684,579 + +2.0 3,684,580 - +0.2 3,684,677 + Pf1N1B4_3297 0.14 +1.2 3,684,713 - Pf1N1B4_3297 0.19 +0.3 3,684,873 + Pf1N1B4_3297 0.43 -1.4 3,684,873 + Pf1N1B4_3297 0.43 +1.9 3,684,873 + Pf1N1B4_3297 0.43 -0.8 3,684,873 + Pf1N1B4_3297 0.43 +0.3 3,684,873 + Pf1N1B4_3297 0.43 +0.0 3,684,874 - Pf1N1B4_3297 0.43 +1.3 3,684,874 - Pf1N1B4_3297 0.43 -0.1 3,684,874 - Pf1N1B4_3297 0.43 +2.0 3,684,876 + Pf1N1B4_3297 0.43 -2.1 3,684,876 + Pf1N1B4_3297 0.43 -2.0 3,684,877 - -0.3 3,684,877 - Pf1N1B4_3297 0.43 +0.4 3,684,877 - Pf1N1B4_3297 0.43 +0.8 3,684,877 - Pf1N1B4_3297 0.43 +0.0 3,685,217 + +0.5 3,685,217 + -0.1 3,685,217 + +0.5 3,685,217 + +1.0 3,685,226 - -1.3 3,685,226 - -0.6
Or see this region's nucleotide sequence