Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3223 and Pf1N1B4_3224 are separated by 167 nucleotides Pf1N1B4_3224 and Pf1N1B4_3225 are separated by 230 nucleotides Pf1N1B4_3225 and Pf1N1B4_3226 are separated by 104 nucleotides Pf1N1B4_3226 and Pf1N1B4_3227 are separated by 144 nucleotides
Pf1N1B4_3223 - Major facilitator family transporter, at 3,608,035 to 3,609,384
_3223
Pf1N1B4_3224 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 3,609,552 to 3,612,398
_3224
Pf1N1B4_3225 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 3,612,629 to 3,612,970
_3225
Pf1N1B4_3226 - FIG00959308: hypothetical protein, at 3,613,075 to 3,613,236
_3226
Pf1N1B4_3227 - hypothetical protein, at 3,613,381 to 3,613,497
_3227
Position (kb)
3609
3610
3611
3612
3613 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.732 kb on - strand, within Pf1N1B4_3223 at 3608.732 kb on - strand, within Pf1N1B4_3223 at 3608.732 kb on - strand at 3608.941 kb on - strand, within Pf1N1B4_3223 at 3609.019 kb on + strand, within Pf1N1B4_3223 at 3609.019 kb on + strand, within Pf1N1B4_3223 at 3609.019 kb on + strand, within Pf1N1B4_3223 at 3609.020 kb on - strand, within Pf1N1B4_3223 at 3609.020 kb on - strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.030 kb on + strand, within Pf1N1B4_3223 at 3609.030 kb on + strand, within Pf1N1B4_3223 at 3609.030 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.113 kb on - strand, within Pf1N1B4_3223 at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.248 kb on - strand, within Pf1N1B4_3223 at 3609.251 kb on + strand at 3609.251 kb on + strand at 3609.251 kb on + strand at 3609.252 kb on - strand at 3609.252 kb on - strand at 3609.252 kb on - strand at 3609.274 kb on - strand at 3609.311 kb on + strand at 3609.311 kb on + strand at 3609.311 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.421 kb on - strand at 3609.421 kb on - strand at 3609.433 kb on + strand at 3609.434 kb on - strand at 3609.532 kb on - strand at 3609.532 kb on - strand at 3609.949 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.995 kb on - strand, within Pf1N1B4_3224 at 3609.995 kb on - strand, within Pf1N1B4_3224 at 3610.165 kb on + strand, within Pf1N1B4_3224 at 3610.166 kb on - strand, within Pf1N1B4_3224 at 3610.166 kb on - strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.301 kb on - strand, within Pf1N1B4_3224 at 3610.301 kb on - strand, within Pf1N1B4_3224 at 3610.301 kb on - strand, within Pf1N1B4_3224 at 3610.586 kb on - strand, within Pf1N1B4_3224 at 3610.586 kb on - strand, within Pf1N1B4_3224 at 3610.804 kb on + strand, within Pf1N1B4_3224 at 3610.804 kb on + strand, within Pf1N1B4_3224 at 3611.086 kb on + strand, within Pf1N1B4_3224 at 3611.087 kb on - strand, within Pf1N1B4_3224 at 3611.359 kb on + strand, within Pf1N1B4_3224 at 3611.519 kb on - strand, within Pf1N1B4_3224 at 3611.791 kb on - strand, within Pf1N1B4_3224 at 3611.791 kb on - strand, within Pf1N1B4_3224 at 3611.791 kb on - strand, within Pf1N1B4_3224 at 3611.829 kb on + strand, within Pf1N1B4_3224 at 3612.146 kb on - strand at 3612.146 kb on - strand at 3612.292 kb on + strand at 3612.431 kb on - strand at 3612.465 kb on + strand at 3612.465 kb on + strand at 3612.465 kb on + strand at 3612.465 kb on + strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.540 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.622 kb on - strand at 3612.622 kb on - strand at 3612.622 kb on - strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.660 kb on + strand at 3613.011 kb on - strand at 3613.011 kb on - strand at 3613.138 kb on - strand at 3613.161 kb on - strand at 3613.161 kb on - strand, within Pf1N1B4_3226 at 3613.268 kb on - strand at 3613.282 kb on + strand at 3613.339 kb on - strand at 3613.343 kb on + strand at 3613.343 kb on + strand at 3613.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 3,608,731 + Pf1N1B4_3223 0.52 -1.7 3,608,731 + Pf1N1B4_3223 0.52 -1.1 3,608,731 + Pf1N1B4_3223 0.52 -0.3 3,608,731 + Pf1N1B4_3223 0.52 +1.9 3,608,731 + Pf1N1B4_3223 0.52 -0.2 3,608,731 + Pf1N1B4_3223 0.52 +0.1 3,608,732 - Pf1N1B4_3223 0.52 +1.1 3,608,732 - Pf1N1B4_3223 0.52 -0.1 3,608,732 - +2.1 3,608,941 - Pf1N1B4_3223 0.67 +1.4 3,609,019 + Pf1N1B4_3223 0.73 -0.3 3,609,019 + Pf1N1B4_3223 0.73 +1.1 3,609,019 + Pf1N1B4_3223 0.73 +0.2 3,609,020 - Pf1N1B4_3223 0.73 +0.1 3,609,020 - Pf1N1B4_3223 0.73 +0.3 3,609,028 + Pf1N1B4_3223 0.74 +0.1 3,609,028 + Pf1N1B4_3223 0.74 +1.0 3,609,028 + Pf1N1B4_3223 0.74 -0.8 3,609,028 + Pf1N1B4_3223 0.74 -0.9 3,609,028 + Pf1N1B4_3223 0.74 -0.3 3,609,028 + Pf1N1B4_3223 0.74 +1.3 3,609,028 + Pf1N1B4_3223 0.74 -1.5 3,609,028 + Pf1N1B4_3223 0.74 +0.8 3,609,029 - Pf1N1B4_3223 0.74 +1.1 3,609,029 - Pf1N1B4_3223 0.74 -1.5 3,609,029 - Pf1N1B4_3223 0.74 +0.3 3,609,029 - Pf1N1B4_3223 0.74 -2.1 3,609,030 + Pf1N1B4_3223 0.74 -2.6 3,609,030 + Pf1N1B4_3223 0.74 -0.3 3,609,030 + Pf1N1B4_3223 0.74 +1.1 3,609,040 + Pf1N1B4_3223 0.74 -1.7 3,609,040 + Pf1N1B4_3223 0.74 -0.5 3,609,040 + Pf1N1B4_3223 0.74 +1.7 3,609,040 + Pf1N1B4_3223 0.74 +0.7 3,609,112 + Pf1N1B4_3223 0.80 +0.7 3,609,112 + Pf1N1B4_3223 0.80 -0.3 3,609,112 + Pf1N1B4_3223 0.80 -0.3 3,609,112 + Pf1N1B4_3223 0.80 -0.7 3,609,113 - Pf1N1B4_3223 0.80 +2.5 3,609,247 + Pf1N1B4_3223 0.90 +0.3 3,609,247 + Pf1N1B4_3223 0.90 +0.4 3,609,247 + -1.3 3,609,247 + Pf1N1B4_3223 0.90 -0.9 3,609,247 + Pf1N1B4_3223 0.90 +0.3 3,609,247 + +1.1 3,609,247 + Pf1N1B4_3223 0.90 +0.3 3,609,248 - Pf1N1B4_3223 0.90 -0.4 3,609,251 + +0.1 3,609,251 + -0.3 3,609,251 + +2.7 3,609,252 - -0.9 3,609,252 - +0.8 3,609,252 - -2.5 3,609,274 - -2.3 3,609,311 + +0.7 3,609,311 + -0.3 3,609,311 + -1.5 3,609,420 + -0.3 3,609,420 + +0.1 3,609,420 + +0.7 3,609,420 + -1.6 3,609,420 + +1.1 3,609,420 + -1.9 3,609,421 - -0.3 3,609,421 - -0.7 3,609,433 + +0.1 3,609,434 - -1.3 3,609,532 - -0.7 3,609,532 - -1.5 3,609,949 + Pf1N1B4_3224 0.14 -0.7 3,609,994 + Pf1N1B4_3224 0.16 -0.6 3,609,994 + Pf1N1B4_3224 0.16 +0.9 3,609,994 + Pf1N1B4_3224 0.16 +0.5 3,609,994 + Pf1N1B4_3224 0.16 -1.7 3,609,994 + Pf1N1B4_3224 0.16 -0.5 3,609,995 - Pf1N1B4_3224 0.16 +1.1 3,609,995 - Pf1N1B4_3224 0.16 -0.9 3,610,165 + Pf1N1B4_3224 0.22 +1.1 3,610,166 - Pf1N1B4_3224 0.22 +0.3 3,610,166 - Pf1N1B4_3224 0.22 -0.3 3,610,300 + Pf1N1B4_3224 0.26 -0.9 3,610,300 + Pf1N1B4_3224 0.26 +0.1 3,610,300 + Pf1N1B4_3224 0.26 +1.5 3,610,300 + Pf1N1B4_3224 0.26 -0.5 3,610,300 + Pf1N1B4_3224 0.26 +0.5 3,610,300 + Pf1N1B4_3224 0.26 -1.1 3,610,301 - Pf1N1B4_3224 0.26 -0.5 3,610,301 - Pf1N1B4_3224 0.26 -0.7 3,610,301 - Pf1N1B4_3224 0.26 -2.1 3,610,586 - Pf1N1B4_3224 0.36 -1.1 3,610,586 - Pf1N1B4_3224 0.36 -0.3 3,610,804 + Pf1N1B4_3224 0.44 -0.7 3,610,804 + Pf1N1B4_3224 0.44 -0.4 3,611,086 + Pf1N1B4_3224 0.54 -0.9 3,611,087 - Pf1N1B4_3224 0.54 -0.7 3,611,359 + Pf1N1B4_3224 0.63 -1.7 3,611,519 - Pf1N1B4_3224 0.69 +0.5 3,611,791 - Pf1N1B4_3224 0.79 -2.4 3,611,791 - Pf1N1B4_3224 0.79 -0.7 3,611,791 - Pf1N1B4_3224 0.79 -0.3 3,611,829 + Pf1N1B4_3224 0.80 -1.9 3,612,146 - -0.0 3,612,146 - -1.5 3,612,292 + -0.7 3,612,431 - -0.2 3,612,465 + -0.2 3,612,465 + +0.7 3,612,465 + +0.8 3,612,465 + +1.1 3,612,466 - +0.9 3,612,466 - -0.6 3,612,466 - +0.4 3,612,466 - +1.5 3,612,466 - -0.5 3,612,466 - -1.1 3,612,466 - -0.3 3,612,466 - -1.1 3,612,540 + -0.5 3,612,621 + +0.3 3,612,621 + +0.5 3,612,621 + +0.1 3,612,621 + +0.1 3,612,621 + -0.6 3,612,622 - +0.8 3,612,622 - -0.8 3,612,622 - -0.5 3,612,625 + +2.7 3,612,625 + +0.6 3,612,625 + +0.1 3,612,625 + +0.3 3,612,625 + -0.4 3,612,625 + -0.2 3,612,625 + +0.9 3,612,625 + -0.4 3,612,625 + -0.4 3,612,626 - -0.4 3,612,626 - -1.1 3,612,626 - +0.7 3,612,626 - -0.7 3,612,626 - -1.7 3,612,626 - +1.5 3,612,626 - +0.1 3,612,626 - +0.1 3,612,626 - -0.4 3,612,626 - +0.5 3,612,626 - +0.5 3,612,626 - +0.3 3,612,626 - +0.6 3,612,626 - +1.1 3,612,626 - -0.7 3,612,626 - +0.6 3,612,626 - +0.1 3,612,626 - +0.8 3,612,626 - -0.5 3,612,628 + +1.0 3,612,628 + +1.6 3,612,628 + -0.7 3,612,628 + +0.4 3,612,628 + -0.5 3,612,628 + -1.0 3,612,628 + +0.6 3,612,628 + +0.1 3,612,628 + +0.1 3,612,628 + -1.7 3,612,628 + +0.8 3,612,628 + -1.5 3,612,629 - -0.1 3,612,629 - -0.1 3,612,629 - -1.0 3,612,629 - +0.4 3,612,629 - +0.8 3,612,629 - +1.1 3,612,629 - +0.4 3,612,629 - +1.6 3,612,629 - +0.3 3,612,629 - +0.7 3,612,629 - +3.1 3,612,629 - -0.9 3,612,629 - +0.6 3,612,629 - -0.1 3,612,629 - -0.4 3,612,629 - +1.7 3,612,629 - -1.5 3,612,629 - +0.6 3,612,660 + -2.1 3,613,011 - +0.3 3,613,011 - +0.3 3,613,138 - +2.1 3,613,161 - +1.1 3,613,161 - Pf1N1B4_3226 0.53 +1.1 3,613,268 - -0.7 3,613,282 + +1.0 3,613,339 - +0.1 3,613,343 + -0.8 3,613,343 + +0.1 3,613,344 - +0.7
Or see this region's nucleotide sequence