Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1733 and Pf1N1B4_1734 are separated by 180 nucleotides Pf1N1B4_1734 and Pf1N1B4_5186 are separated by 347 nucleotides Pf1N1B4_5186 and Pf1N1B4_1735 are separated by 468 nucleotides
Pf1N1B4_1733 - 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,981,191 to 1,982,468
_1733
Pf1N1B4_1734 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 1,982,649 to 1,984,091
_1734
Pf1N1B4_5186 - tRNA-Arg-CCG, at 1,984,439 to 1,984,512
_5186
Pf1N1B4_1735 - hypothetical protein, at 1,984,981 to 1,985,133
_1735
Position (kb)
1982
1983
1984
1985 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.761 kb on + strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1982.015 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.218 kb on + strand, within Pf1N1B4_1733 at 1982.219 kb on - strand, within Pf1N1B4_1733 at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.531 kb on + strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.668 kb on + strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.683 kb on + strand at 1982.683 kb on + strand at 1982.698 kb on + strand at 1982.699 kb on - strand at 1982.699 kb on - strand at 1982.795 kb on - strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1983.187 kb on + strand, within Pf1N1B4_1734 at 1983.205 kb on + strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1984.083 kb on - strand at 1984.083 kb on - strand at 1984.083 kb on - strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.133 kb on - strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.141 kb on - strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.156 kb on - strand at 1984.156 kb on - strand at 1984.156 kb on - strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.201 kb on - strand at 1984.267 kb on + strand at 1984.267 kb on + strand at 1984.267 kb on + strand at 1984.267 kb on + strand at 1984.268 kb on - strand at 1984.282 kb on + strand at 1984.283 kb on - strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.517 kb on - strand at 1984.517 kb on - strand at 1984.517 kb on - strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.521 kb on - strand at 1984.569 kb on - strand at 1984.592 kb on + strand at 1984.593 kb on - strand at 1984.593 kb on - strand at 1984.593 kb on - strand at 1984.695 kb on - strand at 1984.707 kb on - strand at 1984.743 kb on - strand at 1984.743 kb on - strand at 1984.762 kb on - strand at 1984.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 1,981,743 + Pf1N1B4_1733 0.43 +1.4 1,981,743 + Pf1N1B4_1733 0.43 -0.3 1,981,744 - Pf1N1B4_1733 0.43 +0.1 1,981,744 - Pf1N1B4_1733 0.43 -0.4 1,981,744 - Pf1N1B4_1733 0.43 +1.4 1,981,744 - Pf1N1B4_1733 0.43 +0.7 1,981,761 + Pf1N1B4_1733 0.45 -0.3 1,981,762 - Pf1N1B4_1733 0.45 -2.0 1,981,762 - Pf1N1B4_1733 0.45 +0.0 1,981,762 - Pf1N1B4_1733 0.45 +0.9 1,981,762 - Pf1N1B4_1733 0.45 +0.2 1,981,762 - Pf1N1B4_1733 0.45 -0.1 1,981,762 - Pf1N1B4_1733 0.45 -0.4 1,981,762 - Pf1N1B4_1733 0.45 -0.4 1,981,823 - Pf1N1B4_1733 0.49 -0.9 1,981,823 - Pf1N1B4_1733 0.49 -1.1 1,982,015 + Pf1N1B4_1733 0.64 +1.4 1,982,053 + Pf1N1B4_1733 0.67 -1.3 1,982,053 + Pf1N1B4_1733 0.67 +0.4 1,982,210 - Pf1N1B4_1733 0.80 +0.8 1,982,210 - Pf1N1B4_1733 0.80 +0.1 1,982,210 - Pf1N1B4_1733 0.80 -1.9 1,982,210 - Pf1N1B4_1733 0.80 -0.7 1,982,218 + Pf1N1B4_1733 0.80 +0.4 1,982,219 - Pf1N1B4_1733 0.80 +1.1 1,982,468 + +1.7 1,982,468 + +0.4 1,982,468 + -0.6 1,982,468 + +1.4 1,982,468 + -0.7 1,982,468 + -0.2 1,982,469 - +0.6 1,982,469 - +1.4 1,982,469 - -0.4 1,982,469 - +0.1 1,982,469 - -1.7 1,982,469 - +1.9 1,982,531 + +0.3 1,982,532 - +0.6 1,982,532 - +2.4 1,982,532 - +1.6 1,982,650 + +3.4 1,982,650 + +0.4 1,982,650 + +0.0 1,982,650 + +1.4 1,982,650 + -0.6 1,982,650 + +0.3 1,982,650 + +2.8 1,982,651 - +0.1 1,982,651 - -1.1 1,982,651 - -2.6 1,982,668 + -0.1 1,982,669 - -1.7 1,982,669 - -0.3 1,982,669 - -1.4 1,982,669 - +1.0 1,982,683 + +1.4 1,982,683 + +0.4 1,982,698 + -1.1 1,982,699 - +0.0 1,982,699 - +0.6 1,982,795 - Pf1N1B4_1734 0.10 +0.3 1,982,842 + Pf1N1B4_1734 0.13 -1.4 1,982,842 + Pf1N1B4_1734 0.13 -0.1 1,982,842 + Pf1N1B4_1734 0.13 +0.9 1,982,842 + Pf1N1B4_1734 0.13 +0.9 1,982,842 + Pf1N1B4_1734 0.13 +0.6 1,982,843 - Pf1N1B4_1734 0.13 +2.2 1,982,843 - Pf1N1B4_1734 0.13 -0.9 1,982,843 - Pf1N1B4_1734 0.13 -1.1 1,982,843 - Pf1N1B4_1734 0.13 +1.4 1,982,843 - Pf1N1B4_1734 0.13 -2.7 1,982,843 - Pf1N1B4_1734 0.13 -0.9 1,982,843 - Pf1N1B4_1734 0.13 +1.4 1,982,843 - Pf1N1B4_1734 0.13 -2.4 1,982,843 - Pf1N1B4_1734 0.13 -1.4 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,843 - Pf1N1B4_1734 0.13 +0.4 1,982,848 + Pf1N1B4_1734 0.14 -0.9 1,982,848 + Pf1N1B4_1734 0.14 +1.4 1,982,848 + Pf1N1B4_1734 0.14 -2.0 1,982,848 + Pf1N1B4_1734 0.14 -2.1 1,982,848 + Pf1N1B4_1734 0.14 -1.1 1,982,848 + Pf1N1B4_1734 0.14 +0.1 1,982,849 - Pf1N1B4_1734 0.14 +0.8 1,982,849 - Pf1N1B4_1734 0.14 +1.9 1,982,849 - Pf1N1B4_1734 0.14 -0.4 1,983,187 + Pf1N1B4_1734 0.37 +1.0 1,983,205 + Pf1N1B4_1734 0.39 -0.1 1,983,206 - Pf1N1B4_1734 0.39 -1.2 1,983,206 - Pf1N1B4_1734 0.39 -0.6 1,983,206 - Pf1N1B4_1734 0.39 -2.3 1,984,083 - -0.6 1,984,083 - -0.7 1,984,083 - -0.3 1,984,132 + -0.1 1,984,132 + -0.3 1,984,132 + -0.4 1,984,132 + +0.3 1,984,132 + +0.0 1,984,133 - +0.7 1,984,140 + -1.1 1,984,140 + +0.4 1,984,140 + +0.5 1,984,140 + +0.1 1,984,140 + -0.1 1,984,141 - -0.1 1,984,155 + -1.4 1,984,155 + +0.1 1,984,155 + +0.7 1,984,155 + +0.1 1,984,155 + +0.3 1,984,155 + -0.7 1,984,155 + -0.1 1,984,156 - -0.1 1,984,156 - +0.7 1,984,156 - +1.2 1,984,196 + -0.5 1,984,196 + -1.2 1,984,196 + -0.7 1,984,196 + -0.6 1,984,196 + -1.6 1,984,196 + -1.6 1,984,196 + +0.2 1,984,196 + +1.1 1,984,201 - -0.1 1,984,267 + -1.4 1,984,267 + -0.4 1,984,267 + +0.0 1,984,267 + -2.0 1,984,268 - +2.0 1,984,282 + -0.9 1,984,283 - -1.7 1,984,516 + -0.9 1,984,516 + -0.4 1,984,516 + -1.6 1,984,516 + +2.2 1,984,516 + +1.2 1,984,516 + -0.3 1,984,516 + +1.4 1,984,516 + -1.3 1,984,516 + +0.9 1,984,516 + +0.2 1,984,516 + -1.9 1,984,516 + -0.6 1,984,516 + +0.7 1,984,516 + +0.4 1,984,517 - -0.9 1,984,517 - +0.4 1,984,517 - +0.7 1,984,520 + -1.1 1,984,520 + -0.0 1,984,520 + -0.5 1,984,520 + +0.6 1,984,520 + -0.4 1,984,520 + +0.7 1,984,520 + -0.1 1,984,520 + +0.9 1,984,520 + +0.3 1,984,520 + +1.1 1,984,521 - -0.1 1,984,569 - -1.1 1,984,592 + +0.6 1,984,593 - -2.9 1,984,593 - -0.3 1,984,593 - -0.6 1,984,695 - +0.2 1,984,707 - +1.0 1,984,743 - +0.4 1,984,743 - -1.6 1,984,762 - +0.6 1,984,790 - +1.9
Or see this region's nucleotide sequence