Experiment: PYE with Nickel (II) chloride 0.15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00559 and CCNA_00560 are separated by 77 nucleotides CCNA_00560 and CCNA_00561 overlap by 4 nucleotides CCNA_00561 and CCNA_00562 overlap by 4 nucleotides CCNA_00562 and CCNA_00563 overlap by 4 nucleotides CCNA_00563 and CCNA_00564 are separated by 9 nucleotides
CCNA_00559: CCNA_00559 - homoserine O-acetyltransferase, at 574,589 to 575,737
_00559
CCNA_00560: CCNA_00560 - phosphohistidine phosphatase SixA, at 575,815 to 576,327
_00560
CCNA_00561: CCNA_00561 - conserved hypothetical cytosolic protein, at 576,324 to 576,668
_00561
CCNA_00562: CCNA_00562 - dihydroorotate dehydrogenase, at 576,665 to 577,723
_00562
CCNA_00563: CCNA_00563 - SNARE-associated family membrane protein, at 577,720 to 578,457
_00563
CCNA_00564: CCNA_00564 - sensory transduction protein kinase CenK, at 578,467 to 579,972
_00564
Position (kb) 576
577
578 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 575.674 kb on - strand at 575.739 kb on - strand at 575.741 kb on - strand at 575.749 kb on - strand at 575.797 kb on - strand at 575.799 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.807 kb on - strand at 575.807 kb on - strand at 575.809 kb on - strand at 575.818 kb on + strand at 575.835 kb on - strand at 575.841 kb on + strand at 575.847 kb on + strand at 575.847 kb on + strand at 575.860 kb on + strand at 575.860 kb on + strand at 575.868 kb on - strand, within CCNA_00560 at 575.868 kb on - strand, within CCNA_00560 at 575.898 kb on + strand, within CCNA_00560 at 575.900 kb on - strand, within CCNA_00560 at 576.001 kb on + strand, within CCNA_00560 at 576.027 kb on - strand, within CCNA_00560 at 576.031 kb on - strand, within CCNA_00560 at 576.048 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.065 kb on - strand, within CCNA_00560 at 576.175 kb on + strand, within CCNA_00560 at 576.175 kb on + strand, within CCNA_00560 at 576.188 kb on - strand, within CCNA_00560 at 576.191 kb on + strand, within CCNA_00560 at 576.244 kb on + strand, within CCNA_00560 at 576.263 kb on - strand, within CCNA_00560 at 576.270 kb on - strand, within CCNA_00560 at 576.288 kb on + strand at 576.288 kb on + strand at 576.340 kb on - strand at 576.342 kb on + strand at 576.342 kb on + strand at 576.342 kb on + strand at 576.342 kb on + strand at 576.350 kb on - strand at 576.350 kb on - strand at 576.357 kb on + strand at 576.357 kb on + strand at 576.375 kb on + strand, within CCNA_00561 at 576.375 kb on + strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.431 kb on - strand, within CCNA_00561 at 576.455 kb on - strand, within CCNA_00561 at 576.455 kb on - strand, within CCNA_00561 at 576.470 kb on + strand, within CCNA_00561 at 576.478 kb on - strand, within CCNA_00561 at 576.528 kb on + strand, within CCNA_00561 at 576.536 kb on - strand, within CCNA_00561 at 576.536 kb on - strand at 576.536 kb on - strand, within CCNA_00561 at 576.580 kb on - strand, within CCNA_00561 at 576.600 kb on - strand, within CCNA_00561 at 576.615 kb on + strand, within CCNA_00561 at 576.669 kb on + strand at 576.695 kb on + strand at 576.695 kb on + strand at 576.703 kb on - strand at 576.703 kb on - strand at 576.703 kb on - strand at 576.727 kb on - strand at 576.742 kb on + strand at 576.747 kb on + strand at 576.750 kb on - strand at 576.761 kb on - strand at 576.761 kb on - strand at 576.761 kb on - strand at 576.783 kb on + strand, within CCNA_00562 at 576.810 kb on + strand, within CCNA_00562 at 576.818 kb on - strand, within CCNA_00562 at 576.841 kb on - strand, within CCNA_00562 at 576.890 kb on + strand, within CCNA_00562 at 576.905 kb on - strand, within CCNA_00562 at 576.915 kb on + strand, within CCNA_00562 at 576.983 kb on + strand, within CCNA_00562 at 576.998 kb on + strand, within CCNA_00562 at 577.006 kb on - strand, within CCNA_00562 at 577.006 kb on - strand, within CCNA_00562 at 577.054 kb on - strand, within CCNA_00562 at 577.072 kb on - strand, within CCNA_00562 at 577.087 kb on - strand, within CCNA_00562 at 577.097 kb on - strand, within CCNA_00562 at 577.148 kb on + strand, within CCNA_00562 at 577.188 kb on + strand, within CCNA_00562 at 577.188 kb on + strand, within CCNA_00562 at 577.196 kb on - strand, within CCNA_00562 at 577.196 kb on - strand, within CCNA_00562 at 577.205 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.218 kb on - strand, within CCNA_00562 at 577.218 kb on - strand, within CCNA_00562 at 577.262 kb on + strand, within CCNA_00562 at 577.270 kb on - strand, within CCNA_00562 at 577.272 kb on - strand, within CCNA_00562 at 577.311 kb on + strand, within CCNA_00562 at 577.319 kb on - strand, within CCNA_00562 at 577.333 kb on + strand, within CCNA_00562 at 577.333 kb on + strand, within CCNA_00562 at 577.342 kb on - strand, within CCNA_00562 at 577.342 kb on - strand, within CCNA_00562 at 577.347 kb on - strand, within CCNA_00562 at 577.357 kb on + strand, within CCNA_00562 at 577.369 kb on + strand, within CCNA_00562 at 577.370 kb on - strand, within CCNA_00562 at 577.385 kb on - strand, within CCNA_00562 at 577.405 kb on - strand, within CCNA_00562 at 577.439 kb on - strand, within CCNA_00562 at 577.474 kb on + strand, within CCNA_00562 at 577.526 kb on + strand, within CCNA_00562 at 577.571 kb on + strand, within CCNA_00562 at 577.598 kb on + strand, within CCNA_00562 at 577.603 kb on + strand, within CCNA_00562 at 577.603 kb on + strand, within CCNA_00562 at 577.606 kb on - strand, within CCNA_00562 at 577.608 kb on + strand, within CCNA_00562 at 577.613 kb on + strand, within CCNA_00562 at 577.616 kb on - strand, within CCNA_00562 at 577.618 kb on + strand at 577.626 kb on - strand at 577.629 kb on + strand at 577.634 kb on + strand at 577.702 kb on - strand at 577.726 kb on - strand at 577.732 kb on - strand at 577.735 kb on + strand at 577.735 kb on + strand at 577.735 kb on + strand at 577.743 kb on - strand at 577.754 kb on + strand at 577.773 kb on + strand at 577.793 kb on - strand at 577.887 kb on + strand, within CCNA_00563 at 577.887 kb on + strand, within CCNA_00563 at 577.887 kb on + strand, within CCNA_00563 at 577.895 kb on - strand, within CCNA_00563 at 577.899 kb on + strand, within CCNA_00563 at 577.957 kb on - strand, within CCNA_00563 at 578.000 kb on + strand, within CCNA_00563 at 578.035 kb on + strand, within CCNA_00563 at 578.043 kb on - strand, within CCNA_00563 at 578.082 kb on - strand, within CCNA_00563 at 578.082 kb on - strand, within CCNA_00563 at 578.082 kb on - strand, within CCNA_00563 at 578.122 kb on + strand, within CCNA_00563 at 578.134 kb on + strand, within CCNA_00563 at 578.135 kb on + strand, within CCNA_00563 at 578.142 kb on - strand, within CCNA_00563 at 578.153 kb on + strand, within CCNA_00563 at 578.161 kb on - strand, within CCNA_00563 at 578.164 kb on + strand, within CCNA_00563 at 578.172 kb on - strand, within CCNA_00563 at 578.213 kb on + strand, within CCNA_00563 at 578.214 kb on + strand, within CCNA_00563 at 578.221 kb on + strand, within CCNA_00563 at 578.221 kb on + strand, within CCNA_00563 at 578.236 kb on - strand, within CCNA_00563 at 578.236 kb on - strand, within CCNA_00563 at 578.240 kb on + strand, within CCNA_00563 at 578.240 kb on + strand, within CCNA_00563 at 578.248 kb on - strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.296 kb on - strand, within CCNA_00563 at 578.296 kb on - strand, within CCNA_00563 at 578.301 kb on - strand, within CCNA_00563 at 578.301 kb on - strand, within CCNA_00563 at 578.301 kb on - strand, within CCNA_00563 at 578.307 kb on - strand, within CCNA_00563 at 578.307 kb on - strand at 578.307 kb on - strand, within CCNA_00563 at 578.357 kb on + strand, within CCNA_00563
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nickel (II) chloride 0.15 mM remove 575,674 - +1.2 575,739 - -0.8 575,741 - +0.5 575,749 - -0.7 575,797 - -1.4 575,799 + -0.5 575,801 + +0.7 575,801 + -0.7 575,801 + +0.5 575,801 + +0.3 575,801 + +0.3 575,801 + -0.5 575,807 - -0.7 575,807 - +0.6 575,809 - -0.1 575,818 + +0.8 575,835 - +0.9 575,841 + +0.0 575,847 + +0.6 575,847 + +0.6 575,860 + -0.4 575,860 + -1.1 575,868 - CCNA_00560 0.10 -3.0 575,868 - CCNA_00560 0.10 +1.3 575,898 + CCNA_00560 0.16 +0.5 575,900 - CCNA_00560 0.17 -0.7 576,001 + CCNA_00560 0.36 +2.2 576,027 - CCNA_00560 0.41 -0.8 576,031 - CCNA_00560 0.42 -0.5 576,048 + CCNA_00560 0.45 +0.5 576,057 + CCNA_00560 0.47 -2.5 576,057 + CCNA_00560 0.47 +0.4 576,057 + CCNA_00560 0.47 -0.4 576,057 + CCNA_00560 0.47 +0.5 576,065 - CCNA_00560 0.49 -1.4 576,175 + CCNA_00560 0.70 -0.3 576,175 + CCNA_00560 0.70 +0.2 576,188 - CCNA_00560 0.73 -0.2 576,191 + CCNA_00560 0.73 -0.6 576,244 + CCNA_00560 0.84 +0.1 576,263 - CCNA_00560 0.87 -0.9 576,270 - CCNA_00560 0.89 -3.0 576,288 + -1.7 576,288 + +0.7 576,340 - -0.7 576,342 + -1.7 576,342 + -0.4 576,342 + +0.2 576,342 + +0.7 576,350 - -0.6 576,350 - -2.0 576,357 + -0.0 576,357 + -0.0 576,375 + CCNA_00561 0.15 -0.9 576,375 + CCNA_00561 0.15 -0.6 576,383 - CCNA_00561 0.17 -1.4 576,383 - CCNA_00561 0.17 +0.5 576,383 - CCNA_00561 0.17 -0.5 576,383 - CCNA_00561 0.17 +0.1 576,431 - CCNA_00561 0.31 -0.0 576,455 - CCNA_00561 0.38 +0.6 576,455 - CCNA_00561 0.38 -0.2 576,470 + CCNA_00561 0.42 +1.2 576,478 - CCNA_00561 0.45 +0.5 576,528 + CCNA_00561 0.59 +0.1 576,536 - CCNA_00561 0.61 +1.0 576,536 - -0.0 576,536 - CCNA_00561 0.61 -0.1 576,580 - CCNA_00561 0.74 -1.9 576,600 - CCNA_00561 0.80 -0.3 576,615 + CCNA_00561 0.84 -0.3 576,669 + +0.3 576,695 + -0.4 576,695 + -2.3 576,703 - +0.0 576,703 - -1.3 576,703 - -0.9 576,727 - +0.2 576,742 + -1.0 576,747 + +0.4 576,750 - +0.4 576,761 - -1.0 576,761 - -0.9 576,761 - -1.5 576,783 + CCNA_00562 0.11 -1.9 576,810 + CCNA_00562 0.14 -0.8 576,818 - CCNA_00562 0.14 +0.4 576,841 - CCNA_00562 0.17 +0.5 576,890 + CCNA_00562 0.21 -0.6 576,905 - CCNA_00562 0.23 +1.0 576,915 + CCNA_00562 0.24 -1.2 576,983 + CCNA_00562 0.30 -0.9 576,998 + CCNA_00562 0.31 -1.0 577,006 - CCNA_00562 0.32 -1.3 577,006 - CCNA_00562 0.32 -1.6 577,054 - CCNA_00562 0.37 -0.9 577,072 - CCNA_00562 0.38 -0.2 577,087 - CCNA_00562 0.40 +0.1 577,097 - CCNA_00562 0.41 -2.4 577,148 + CCNA_00562 0.46 -1.5 577,188 + CCNA_00562 0.49 -0.5 577,188 + CCNA_00562 0.49 +0.2 577,196 - CCNA_00562 0.50 -2.5 577,196 - CCNA_00562 0.50 -1.0 577,205 + CCNA_00562 0.51 -2.1 577,210 + CCNA_00562 0.51 -0.5 577,210 + CCNA_00562 0.51 -1.4 577,210 + CCNA_00562 0.51 -1.0 577,210 + CCNA_00562 0.51 -0.5 577,210 + CCNA_00562 0.51 -0.6 577,210 + CCNA_00562 0.51 -0.8 577,218 - CCNA_00562 0.52 -1.4 577,218 - CCNA_00562 0.52 -1.5 577,262 + CCNA_00562 0.56 -0.6 577,270 - CCNA_00562 0.57 -1.6 577,272 - CCNA_00562 0.57 -1.9 577,311 + CCNA_00562 0.61 -1.6 577,319 - CCNA_00562 0.62 -1.1 577,333 + CCNA_00562 0.63 +0.3 577,333 + CCNA_00562 0.63 -0.4 577,342 - CCNA_00562 0.64 -2.1 577,342 - CCNA_00562 0.64 +0.3 577,347 - CCNA_00562 0.64 -0.8 577,357 + CCNA_00562 0.65 -1.2 577,369 + CCNA_00562 0.66 +0.8 577,370 - CCNA_00562 0.67 -1.7 577,385 - CCNA_00562 0.68 +1.3 577,405 - CCNA_00562 0.70 -1.4 577,439 - CCNA_00562 0.73 -1.6 577,474 + CCNA_00562 0.76 -0.8 577,526 + CCNA_00562 0.81 -0.9 577,571 + CCNA_00562 0.86 -1.7 577,598 + CCNA_00562 0.88 -1.1 577,603 + CCNA_00562 0.89 -1.2 577,603 + CCNA_00562 0.89 -0.3 577,606 - CCNA_00562 0.89 -1.0 577,608 + CCNA_00562 0.89 -0.2 577,613 + CCNA_00562 0.90 +0.0 577,616 - CCNA_00562 0.90 -0.6 577,618 + -1.6 577,626 - -0.1 577,629 + -0.3 577,634 + -0.6 577,702 - -1.3 577,726 - -1.4 577,732 - -0.9 577,735 + -0.3 577,735 + -0.7 577,735 + +0.3 577,743 - -2.2 577,754 + -0.7 577,773 + +0.5 577,793 - +0.5 577,887 + CCNA_00563 0.23 +1.8 577,887 + CCNA_00563 0.23 +0.0 577,887 + CCNA_00563 0.23 +1.0 577,895 - CCNA_00563 0.24 -1.3 577,899 + CCNA_00563 0.24 +0.6 577,957 - CCNA_00563 0.32 +1.3 578,000 + CCNA_00563 0.38 +0.2 578,035 + CCNA_00563 0.43 +1.3 578,043 - CCNA_00563 0.44 -0.8 578,082 - CCNA_00563 0.49 +0.4 578,082 - CCNA_00563 0.49 +0.8 578,082 - CCNA_00563 0.49 +0.3 578,122 + CCNA_00563 0.54 +1.4 578,134 + CCNA_00563 0.56 -0.1 578,135 + CCNA_00563 0.56 -0.3 578,142 - CCNA_00563 0.57 +0.1 578,153 + CCNA_00563 0.59 -1.2 578,161 - CCNA_00563 0.60 -0.5 578,164 + CCNA_00563 0.60 -0.2 578,172 - CCNA_00563 0.61 +1.1 578,213 + CCNA_00563 0.67 -1.1 578,214 + CCNA_00563 0.67 +0.0 578,221 + CCNA_00563 0.68 -0.5 578,221 + CCNA_00563 0.68 -1.4 578,236 - CCNA_00563 0.70 -1.3 578,236 - CCNA_00563 0.70 +0.1 578,240 + CCNA_00563 0.70 +0.6 578,240 + CCNA_00563 0.70 -0.3 578,248 - CCNA_00563 0.72 +0.4 578,293 + CCNA_00563 0.78 -0.1 578,293 + CCNA_00563 0.78 +0.6 578,293 + CCNA_00563 0.78 +0.1 578,293 + CCNA_00563 0.78 +0.4 578,293 + CCNA_00563 0.78 +0.3 578,296 - CCNA_00563 0.78 -0.2 578,296 - CCNA_00563 0.78 -1.4 578,301 - CCNA_00563 0.79 -0.6 578,301 - CCNA_00563 0.79 -0.1 578,301 - CCNA_00563 0.79 -0.0 578,307 - CCNA_00563 0.80 -0.6 578,307 - +0.8 578,307 - CCNA_00563 0.80 +1.0 578,357 + CCNA_00563 0.86 -0.1
Or see this region's nucleotide sequence