Experiment: KB-half, Pseudo5 supernatant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25825 and AO356_25830 are separated by 15 nucleotides AO356_25830 and AO356_25835 are separated by 22 nucleotides AO356_25835 and AO356_25840 overlap by 4 nucleotides
AO356_25825: AO356_25825 - hypothetical protein, at 5,839,134 to 5,840,825
_25825
AO356_25830: AO356_25830 - DNA repair protein, at 5,840,841 to 5,841,488
_25830
AO356_25835: AO356_25835 - hypothetical protein, at 5,841,511 to 5,841,990
_25835
AO356_25840: AO356_25840 - peptidylprolyl isomerase, at 5,841,987 to 5,842,931
_25840
Position (kb)
5841
5842 Strain fitness (log2 ratio)
-2
-1
0
1 at 5840.620 kb on - strand, within AO356_25825 at 5840.620 kb on - strand, within AO356_25825 at 5840.620 kb on - strand, within AO356_25825 at 5840.620 kb on - strand, within AO356_25825 at 5840.712 kb on + strand at 5840.712 kb on + strand at 5840.713 kb on - strand at 5840.859 kb on + strand at 5840.860 kb on - strand at 5841.310 kb on - strand, within AO356_25830 at 5841.310 kb on - strand, within AO356_25830 at 5841.901 kb on + strand, within AO356_25835 at 5841.902 kb on - strand, within AO356_25835 at 5841.913 kb on + strand, within AO356_25835 at 5841.913 kb on + strand, within AO356_25835 at 5842.363 kb on - strand, within AO356_25840 at 5842.363 kb on - strand, within AO356_25840 at 5842.425 kb on + strand, within AO356_25840 at 5842.425 kb on + strand, within AO356_25840 at 5842.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo5 supernatant remove 5,840,620 - AO356_25825 0.88 -2.2 5,840,620 - AO356_25825 0.88 -0.2 5,840,620 - AO356_25825 0.88 -1.2 5,840,620 - AO356_25825 0.88 +1.4 5,840,712 + -1.6 5,840,712 + -1.2 5,840,713 - -0.2 5,840,859 + +0.4 5,840,860 - -0.2 5,841,310 - AO356_25830 0.72 -2.2 5,841,310 - AO356_25830 0.72 -1.2 5,841,901 + AO356_25835 0.81 -1.2 5,841,902 - AO356_25835 0.81 +0.4 5,841,913 + AO356_25835 0.84 -0.2 5,841,913 + AO356_25835 0.84 +0.8 5,842,363 - AO356_25840 0.40 +0.8 5,842,363 - AO356_25840 0.40 -1.6 5,842,425 + AO356_25840 0.46 -1.2 5,842,425 + AO356_25840 0.46 -1.2 5,842,852 - +0.4
Or see this region's nucleotide sequence