Experiment: KB-half, Pseudo5 supernatant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25020 and AO356_25025 are separated by 251 nucleotides AO356_25025 and AO356_25030 overlap by 4 nucleotides AO356_25030 and AO356_25035 are separated by 40 nucleotides
AO356_25020: AO356_25020 - hypothetical protein, at 5,668,743 to 5,669,927
_25020
AO356_25025: AO356_25025 - cytochrome C oxidase Cbb3, at 5,670,179 to 5,671,675
_25025
AO356_25030: AO356_25030 - uroporphyrin-III methyltransferase, at 5,671,672 to 5,672,514
_25030
AO356_25035: AO356_25035 - heme d1 biosynthesis radical SAM protein NirJ, at 5,672,555 to 5,673,733
_25035
Position (kb) 5670
5671
5672 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5669.400 kb on + strand, within AO356_25020 at 5669.436 kb on + strand, within AO356_25020 at 5669.466 kb on + strand, within AO356_25020 at 5669.467 kb on - strand, within AO356_25020 at 5669.467 kb on - strand, within AO356_25020 at 5669.467 kb on - strand, within AO356_25020 at 5669.467 kb on - strand, within AO356_25020 at 5669.487 kb on + strand, within AO356_25020 at 5669.487 kb on + strand, within AO356_25020 at 5669.488 kb on - strand, within AO356_25020 at 5669.691 kb on + strand, within AO356_25020 at 5669.691 kb on + strand, within AO356_25020 at 5669.692 kb on - strand, within AO356_25020 at 5669.692 kb on - strand, within AO356_25020 at 5669.692 kb on - strand, within AO356_25020 at 5669.692 kb on - strand, within AO356_25020 at 5669.724 kb on + strand, within AO356_25020 at 5669.775 kb on + strand, within AO356_25020 at 5669.839 kb on - strand at 5669.906 kb on - strand at 5669.926 kb on - strand at 5669.951 kb on + strand at 5669.966 kb on + strand at 5669.966 kb on + strand at 5669.966 kb on + strand at 5669.967 kb on - strand at 5669.967 kb on - strand at 5669.967 kb on - strand at 5670.063 kb on + strand at 5670.064 kb on - strand at 5670.064 kb on - strand at 5670.064 kb on - strand at 5670.225 kb on + strand at 5670.225 kb on + strand at 5670.226 kb on - strand at 5670.316 kb on + strand at 5670.317 kb on - strand at 5670.331 kb on - strand, within AO356_25025 at 5670.621 kb on + strand, within AO356_25025 at 5670.621 kb on + strand, within AO356_25025 at 5670.622 kb on - strand, within AO356_25025 at 5670.771 kb on + strand, within AO356_25025 at 5670.771 kb on + strand, within AO356_25025 at 5670.772 kb on - strand, within AO356_25025 at 5670.889 kb on - strand, within AO356_25025 at 5671.096 kb on - strand, within AO356_25025 at 5671.096 kb on - strand, within AO356_25025 at 5671.129 kb on - strand, within AO356_25025 at 5671.169 kb on + strand, within AO356_25025 at 5671.389 kb on + strand, within AO356_25025 at 5671.581 kb on + strand at 5671.581 kb on + strand at 5671.581 kb on + strand at 5671.581 kb on + strand at 5671.582 kb on - strand at 5671.961 kb on + strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5671.962 kb on - strand, within AO356_25030 at 5672.093 kb on + strand, within AO356_25030 at 5672.162 kb on + strand, within AO356_25030 at 5672.162 kb on + strand, within AO356_25030 at 5672.163 kb on - strand, within AO356_25030 at 5672.309 kb on + strand, within AO356_25030
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo5 supernatant remove 5,669,400 + AO356_25020 0.55 -0.6 5,669,436 + AO356_25020 0.58 +0.5 5,669,466 + AO356_25020 0.61 +0.4 5,669,467 - AO356_25020 0.61 -1.2 5,669,467 - AO356_25020 0.61 +0.7 5,669,467 - AO356_25020 0.61 -0.8 5,669,467 - AO356_25020 0.61 +0.8 5,669,487 + AO356_25020 0.63 +0.4 5,669,487 + AO356_25020 0.63 +1.8 5,669,488 - AO356_25020 0.63 -0.6 5,669,691 + AO356_25020 0.80 +1.8 5,669,691 + AO356_25020 0.80 +0.4 5,669,692 - AO356_25020 0.80 +0.9 5,669,692 - AO356_25020 0.80 +0.4 5,669,692 - AO356_25020 0.80 +1.0 5,669,692 - AO356_25020 0.80 +0.7 5,669,724 + AO356_25020 0.83 -1.2 5,669,775 + AO356_25020 0.87 +1.5 5,669,839 - -0.3 5,669,906 - -1.2 5,669,926 - -1.4 5,669,951 + +0.4 5,669,966 + +1.3 5,669,966 + -0.8 5,669,966 + +0.1 5,669,967 - -0.2 5,669,967 - +0.5 5,669,967 - -0.6 5,670,063 + -0.7 5,670,064 - +1.2 5,670,064 - +0.4 5,670,064 - +2.0 5,670,225 + +0.2 5,670,225 + +3.1 5,670,226 - +0.5 5,670,316 + -0.4 5,670,317 - +0.6 5,670,331 - AO356_25025 0.10 +0.6 5,670,621 + AO356_25025 0.30 -0.2 5,670,621 + AO356_25025 0.30 -0.4 5,670,622 - AO356_25025 0.30 -1.2 5,670,771 + AO356_25025 0.40 -0.2 5,670,771 + AO356_25025 0.40 +0.7 5,670,772 - AO356_25025 0.40 +0.4 5,670,889 - AO356_25025 0.47 +0.8 5,671,096 - AO356_25025 0.61 -2.8 5,671,096 - AO356_25025 0.61 -1.2 5,671,129 - AO356_25025 0.63 +0.8 5,671,169 + AO356_25025 0.66 -0.2 5,671,389 + AO356_25025 0.81 -0.2 5,671,581 + +0.7 5,671,581 + -1.2 5,671,581 + +2.4 5,671,581 + +0.4 5,671,582 - -0.8 5,671,961 + AO356_25030 0.34 +1.3 5,671,962 - AO356_25030 0.34 +0.4 5,671,962 - AO356_25030 0.34 +0.7 5,671,962 - AO356_25030 0.34 -1.8 5,671,962 - AO356_25030 0.34 -0.2 5,672,093 + AO356_25030 0.50 +0.4 5,672,162 + AO356_25030 0.58 -0.9 5,672,162 + AO356_25030 0.58 -0.8 5,672,163 - AO356_25030 0.58 +1.0 5,672,309 + AO356_25030 0.76 -1.2
Or see this region's nucleotide sequence