Experiment: KB-half, Pseudo5 supernatant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23250 and AO356_23255 are separated by 201 nucleotides AO356_23255 and AO356_23260 overlap by 4 nucleotides AO356_23260 and AO356_23265 overlap by 4 nucleotides AO356_23265 and AO356_23270 overlap by 4 nucleotides
AO356_23250: AO356_23250 - hypothetical protein, at 5,203,411 to 5,203,848
_23250
AO356_23255: AO356_23255 - cation:proton antiporter, at 5,204,050 to 5,204,412
_23255
AO356_23260: AO356_23260 - cation:proton antiporter, at 5,204,409 to 5,204,687
_23260
AO356_23265: AO356_23265 - cation:proton antiporter, at 5,204,684 to 5,205,172
_23265
AO356_23270: AO356_23270 - cation:proton antiporter, at 5,205,169 to 5,206,854
_23270
Position (kb) 5204
5205
5206 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5203.792 kb on + strand, within AO356_23250 at 5203.875 kb on + strand at 5203.875 kb on + strand at 5203.876 kb on - strand at 5203.876 kb on - strand at 5203.876 kb on - strand at 5203.876 kb on - strand at 5204.045 kb on - strand at 5204.500 kb on + strand, within AO356_23260 at 5204.501 kb on - strand, within AO356_23260 at 5204.501 kb on - strand, within AO356_23260 at 5204.501 kb on - strand, within AO356_23260 at 5204.516 kb on - strand, within AO356_23260 at 5204.557 kb on + strand, within AO356_23260 at 5204.557 kb on + strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.564 kb on - strand, within AO356_23260 at 5204.564 kb on - strand, within AO356_23260 at 5204.715 kb on + strand at 5204.715 kb on + strand at 5204.716 kb on - strand at 5204.716 kb on - strand at 5204.716 kb on - strand at 5204.927 kb on + strand, within AO356_23265 at 5204.928 kb on - strand, within AO356_23265 at 5204.928 kb on - strand, within AO356_23265 at 5204.928 kb on - strand, within AO356_23265 at 5205.105 kb on - strand, within AO356_23265 at 5205.105 kb on - strand, within AO356_23265 at 5205.233 kb on - strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.246 kb on - strand at 5205.246 kb on - strand at 5205.246 kb on - strand at 5205.285 kb on + strand at 5205.612 kb on + strand, within AO356_23270 at 5205.612 kb on + strand, within AO356_23270 at 5205.612 kb on + strand, within AO356_23270 at 5205.612 kb on + strand, within AO356_23270 at 5205.733 kb on - strand, within AO356_23270 at 5205.836 kb on + strand, within AO356_23270 at 5205.836 kb on + strand, within AO356_23270 at 5205.837 kb on - strand, within AO356_23270 at 5205.837 kb on - strand, within AO356_23270 at 5205.837 kb on - strand, within AO356_23270 at 5205.992 kb on + strand, within AO356_23270 at 5205.993 kb on - strand, within AO356_23270 at 5206.073 kb on + strand, within AO356_23270 at 5206.073 kb on + strand, within AO356_23270 at 5206.073 kb on + strand, within AO356_23270 at 5206.073 kb on + strand, within AO356_23270 at 5206.133 kb on + strand, within AO356_23270 at 5206.134 kb on - strand, within AO356_23270 at 5206.134 kb on - strand, within AO356_23270 at 5206.134 kb on - strand, within AO356_23270
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo5 supernatant remove 5,203,792 + AO356_23250 0.87 +1.0 5,203,875 + -1.0 5,203,875 + -1.0 5,203,876 - -2.0 5,203,876 - -1.0 5,203,876 - +0.7 5,203,876 - +0.0 5,204,045 - +0.6 5,204,500 + AO356_23260 0.33 +0.3 5,204,501 - AO356_23260 0.33 +0.0 5,204,501 - AO356_23260 0.33 -0.6 5,204,501 - AO356_23260 0.33 +2.8 5,204,516 - AO356_23260 0.38 +0.0 5,204,557 + AO356_23260 0.53 +0.3 5,204,557 + AO356_23260 0.53 -0.4 5,204,558 - AO356_23260 0.53 +1.0 5,204,558 - AO356_23260 0.53 +1.0 5,204,558 - AO356_23260 0.53 +1.3 5,204,558 - AO356_23260 0.53 -0.5 5,204,558 - AO356_23260 0.53 +0.6 5,204,563 + AO356_23260 0.55 +0.6 5,204,563 + AO356_23260 0.55 -1.0 5,204,563 + AO356_23260 0.55 +1.2 5,204,563 + AO356_23260 0.55 -0.4 5,204,563 + AO356_23260 0.55 +0.5 5,204,564 - AO356_23260 0.56 +0.0 5,204,564 - AO356_23260 0.56 +0.6 5,204,715 + +1.0 5,204,715 + -1.0 5,204,716 - -1.2 5,204,716 - -1.0 5,204,716 - +2.2 5,204,927 + AO356_23265 0.50 -1.3 5,204,928 - AO356_23265 0.50 -0.7 5,204,928 - AO356_23265 0.50 +0.0 5,204,928 - AO356_23265 0.50 +0.0 5,205,105 - AO356_23265 0.86 -0.7 5,205,105 - AO356_23265 0.86 -2.0 5,205,233 - +1.4 5,205,245 + -0.1 5,205,245 + -0.7 5,205,245 + +0.6 5,205,245 + +0.6 5,205,245 + -0.4 5,205,245 + -1.0 5,205,246 - +0.8 5,205,246 - +1.1 5,205,246 - +0.8 5,205,285 + -1.0 5,205,612 + AO356_23270 0.26 -1.0 5,205,612 + AO356_23270 0.26 -2.0 5,205,612 + AO356_23270 0.26 -0.6 5,205,612 + AO356_23270 0.26 +0.0 5,205,733 - AO356_23270 0.33 +0.6 5,205,836 + AO356_23270 0.40 -0.1 5,205,836 + AO356_23270 0.40 +1.0 5,205,837 - AO356_23270 0.40 -1.0 5,205,837 - AO356_23270 0.40 +1.8 5,205,837 - AO356_23270 0.40 +0.0 5,205,992 + AO356_23270 0.49 +0.6 5,205,993 - AO356_23270 0.49 +1.6 5,206,073 + AO356_23270 0.54 -1.0 5,206,073 + AO356_23270 0.54 +0.3 5,206,073 + AO356_23270 0.54 +0.4 5,206,073 + AO356_23270 0.54 +1.2 5,206,133 + AO356_23270 0.57 +0.0 5,206,134 - AO356_23270 0.57 +2.5 5,206,134 - AO356_23270 0.57 +0.4 5,206,134 - AO356_23270 0.57 +0.0
Or see this region's nucleotide sequence